檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【人淋巴毒素β(LTB)ELISA試劑盒廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人淋巴毒素β(LTB)ELISA試劑盒廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人淋巴毒素β(LTB)ELISA試劑盒廠家】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計(jì)算時(shí)請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【人淋巴毒素β(LTB)ELISA試劑盒廠家】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

9236152  Locator Plate, Tips, BR Rapid Plate                   CNY                             

9236147  Disposal Chute, side, BR Rapid Plate                 CNY                             

9230778  Spritzschutz Waschstation Plato 7            CNY                             

9230776  Scannerdeckel Rose 80             CNY                             

9230405  Pipetting Position Ground Plate                CNY                              【人淋巴毒素β(LTB)ELISA試劑盒廠家】

9230189  Safety shield for size 0               CNY                             

9022808  LED Barrel, QXA                CNY                             

9238656  Cable, 50-channel             CNY                             

9238647  Mechanical lock with optic, left                CNY                             

9238631  Keypad, foil                CNY                             

9242083  Insert, 2.0ml v2, samplecarr. (24), Qsym                   CNY                             

9242071  External BCR, Metrologic, MS3780, AP            CNY                             

9242062  Cover, evaporation trap, IQ/OQ, QS                 CNY                             

9241893  QIArent&go - Rent out fee QIAxtractor           CNY                             

9241826  Pyro Q24, Premium Agreement       "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9241817  Pyro Q96 MDa, PM Agreement                CNY                             

9241174  Power supply, main, 24V, QX            CNY                             

9241008  QIArent&go - Rent out fee QIAsymphony                 CNY                             

9240958  Gripper, handler, Qsym             CNY                             

9240818  Performance, certification, EZ1                CNY                             

9234855  Lid coupling piece for washer liquid                   CNY                              【人淋巴毒素β(LTB)ELISA試劑盒廠家】

9240784  Update, Weigh Cell, AP98                  CNY                             

9240773  Assy, Shaker, AP96            CNY                             

9241775  QIAxtractor, Installation The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the QIAxtractor systems hardware ａnd trains the user on the operation of the software. After the training the user will be able to run ａnd install application programs ａnd conduct a basic troubleshooting. All travel ａnd labor costs are included. The service is documented in accordance with GMP/GLP requirements. CNY                             

9241631  Capper Mounting Kit, AP                   CNY                             

9243209  APAC-HC2 Service Package 5yrs                CNY                             

9243043  Panel, transparent, Qsym                  CNY                             

9242767  Cogwheel, y-drive, portal, Qsym               CNY                             

9242762  QS RGQ, Basic Agreement       "Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 5 business days

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242716  Validation Support, 5d, QIAsymphony     The QIAGEN QIAsymphony Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Applications Specialist.  A QIAGEN specialist will provide an introduction to the QIAsymphony system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on user support ａnd QIAsymphony instrument operation. The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product. Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to include in the validation (Casework200, Casework500, Reference200, etc.).       CNY                             

9242548  Cheetah Upgrade Kit, AP98              CNY                             

9242532  QIAsymphony AS, Installation The Installation, QIAsymphony AS facilitates the quick implementation of the QIAsymphony into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAsymphony instrument ａnd provides the user with an introductory training. The installation includes or labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document. CNY                             

9242155  Loaner system (1 time rental)          CNY                             

QY-00270 MTR-1A (Melatonin receptor-1A)褪黑素受體（抗原）                  0.5mg/6501mg/1100                                  

QY-03340 MTLC (c-Myc target from laryngeal cancer cells)又稱：MYC致癌基因（抗原）                  0.5mg/6501mg/1100                                   【人淋巴毒素β(LTB)ELISA試劑盒廠家】

QY-02240 N-coR1 （Nuclear receptor co-repressor 1） 核受體輔助抑制因子（抗原）            0.5mg/6501mg/1100                                 

QY-00060 Nestin巢蛋白（神經(jīng)上皮干細(xì)胞蛋白）（抗原）                  0.5mg/6501mg/1100                                  

QY-00080 Nestin巢蛋白（神經(jīng)上皮干細(xì)胞蛋白）（抗原）                  0.5mg/6501mg/1100                                  

QY-02890 neurofasciu-155神經(jīng)束蛋白-155             0.5mg/6501mg/1100                                  

QY-00670 NGF-β神經(jīng)生長因子-β(多肽片斷抗原)                0.5mg/6501mg/1100                                  

QY-01610 NGF-R(p75NTR)神經(jīng)生長因子受體（抗原）                  0.5mg/6501mg/1100                                  

QY-00740 NIK，NF-kappaB-Inducing KinaseNFkB誘導(dǎo)的激酶（抗原）                 0.5mg/6501mg/1100                                  

QY-05170 ATF1 (Activating Transcription Factor1)活化復(fù)制因子1                   0.5mg/650                                  

QY-00700 NKB （Neurokinins B）神經(jīng)激肽B（抗原）                 0.5mg/6501mg/1100                                  

QY-01660 NKR ； Neurokin B receptor (Neuromedin K receptor; NK-4 receptor)神經(jīng)激肽-B受體（抗原）             0.5mg/6501mg/1100                                  

QY-00750 Nociceptin孤菲肽（痛敏肽）（抗原）                                                       

QY-01810 Nociceptin receptor孤菲肽受體（痛敏肽受體）（抗原）            0.5mg/6501mg/1100                                  

QY-01340 Nogo-A軸索過度生長抑制因子-A（抗原）                   0.5mg/6501mg/1100                                  

QY-01670 Nogo-66/NEP1-40Nogo-66肽(1-40)多肽                   0.5mg/11001mg/1800                               

QY-01290 Nogo R軸索過度生長抑制因子受體（多肽抗原）              0.5mg/6501mg/1100                                  

QY-08920 Bad (BCL-xL/BCL-2-associated death promoter)相關(guān)死亡促進(jìn)因子Bad抗原                0.5mg/6501mg/1100                                  【人淋巴毒素β(LTB)ELISA試劑盒廠家】

QY-01560 NOS-1(bNOS:neuronal NOS:nNOS:Nitric Oxide synthase-1）一氧化氮合成酶-1（抗原）            0.5mg/6501mg/1100                                  

QY-01620 NOS-2 (iNOS) Nitric Oxide Synthase,Inducible 一氧化氮合成酶-2（抗原）                   0.5mg/6501mg/1100                                 

QY-01630 NOS-3 (eNOS) endotheli cell NOS一氧化氮合成酶-3（抗原）               0.5mg/6501mg/1100                                  

QY-00710 NPY （ Neuropeptide Y）神經(jīng)肽 Y（抗原）               0.5mg/6501mg/1100                                  

QY-02220 NR2B （Glutamate receptor）谷氨酸受體（抗原）           0.5mg/6501mg/1100                                  

QY-01600 NT-3神經(jīng)生長因子-3（抗原）                0.5mg/6501mg/1100                                  

QY-01580 NT-4,NT-5神經(jīng)生長因子4/5（抗原）             0.5mg/6501mg/1100                                  

QY-00730 NTN （Neurturin） 神經(jīng)生長因子（抗原）                0.5mg/6501mg/1100                                  

QY-01780 Nurr1核受體相關(guān)因子-1（抗原）                  0.5mg/6501mg/1100                                  

QY-03720 CD73 (5´-nucleotidase cytosolicⅡ)胞漿-5´-核苷酸酶-Ⅱ 抗原              0.5mg/6501mg/1100                                  

QY-02400 OGP (Ostegenic Growth Peptide)成骨生長肽 (骨生長激素肽)(多肽)           0.5mg/6501mg/1100                                  

QY-00190 OPN骨橋蛋白（多肽抗原）                    0.5mg/6501mg/1100                                  

QY-00330 P53腫瘤抑制基因（抗原）            0.5mg/6501mg/1100