1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人糖基化依賴的細胞黏附分子(GlyCAM-1)ELISA試劑盒廠家】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強,重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人糖基化依賴的細胞黏附分子(GlyCAM-1)ELISA試劑盒廠家】齊一生物科技歡迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人糖基化依賴的細胞黏附分子(GlyCAM-1)ELISA試劑盒廠家】齊一生物供應(yīng)的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內(nèi)進行檢測.先將生物素標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標記物如酶標結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標準品,對ad標準品的靈敏度為0.05ng/ml對ay標準品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標準品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標準,O.D.值大于或等于Cut-Off值的樣品被認為是初試陽性. 【人糖基化依賴的細胞黏附分子(GlyCAM-1)ELISA試劑盒廠家】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學(xué)生物試劑盒、金標法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養(yǎng)基、細胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9238708  Arrow, direction of rotation              CNY                             

9232100  Front blind for size 200              CNY                             

9238904  Magnet motor, BS96                 CNY                             

9238894  PSP-CSU1 cable, BS15                CNY                             

9238694  Temperature switch, fan control              CNY                             

9231892  Gasket, 1-8 connector, BR8000                 CNY                             

9238685  Mains switch ETA, 10 A             CNY                              【人糖基化依賴的細胞黏附分子(GlyCAM-1)ELISA試劑盒廠家】

9231656  Holder, CCD Camera BL-185             CNY                             

9231538  Needle, W-Drive, DISU, BR8             CNY                             

9014876  Microplate Station Adapter              CNY                             

9014556  Shaker Adapter 8000  24-tubes  13ml                   CNY                             

9014499  Disposable Tip Station, BR8, RD v1.0                CNY                             

9022574  Strut assembly, Hood, SP, right, Qsym               CNY                             

9238348  Upgrade, Precision Pippeting  Precision pipetting upgrade includes onsite installation ａnd calibration visit including all travel ａnd labor to install the precision pipetting system, as well as the following components: 4x100ul syringes, 4x 3 Port Valves, 24x96 10ul Disposable Tips ａnd a precision Pipetting Calibration Kit.       CNY                             

9022379  Notebook, RGQ                 CNY                             

9021984  Transport lock, QXA          CNY                             

9021892  Bracket, optical sensor, angled, QIAC               CNY                             

9021613  Kit, upgrade QSSP Cabinet, Qsym             CNY                             

9238605  Centrifuge Motor, complete             CNY                             

9238552  RS232 box, online fill ready, RapidPlate           CNY                             

9238253  Syringe Seal               CNY                             

9238222  De-Bubbler Top                  CNY                             

9240674  Clamp Kit, Sensor, Reagent Jar, AP96/196                 CNY                              【人糖基化依賴的細胞黏附分子(GlyCAM-1)ELISA試劑盒廠家】

9240665  Nut, Flangeless,1/4-28, White, AP             CNY                             

9240656  Rebuild Kit, Gast Pump, AP96          CNY                             

9240646  External BCR, 2D, HHP, AP                CNY                             

9240619  Fuse, TE5, 2.0A, PCB, AP96/196                 CNY                             

9240610  Hall Effects Sensor, Latch Lock, AP98                CNY                             

9240609  Fiber Optic Sensor, Sub-unit, AP98           CNY                             

9240587  Vacuum Switch, Pump, AP96            CNY                             

9240579  Vacuum Switch Assy, Shaker, AP96           CNY                             

9240577  Cable Assy, Capper, X52 CW, AP96/196              CNY                             

9240525  Cable, Motor Axis #2, Elevator, AP98                CNY                             

QY-02690 FoxP3叉頭蛋白3-T細胞轉(zhuǎn)錄因子（多肽抗原）                  0.5mg/6501mg/11005mg/4800                                  

QY-03250 GAD-65 (glutamate decarboxylase)谷氨酸脫羧酶-65(抗原)                    0.5mg/8501mg/1300                                  

QY-01540 GAP-43 ( Growth associated protein-43 )生長相關(guān)蛋白-43（抗原）             0.5mg/6501mg/1100                                  

QY-00100 Gastrin胃泌素（抗原）                   0.5mg/6501mg/1100                                  

QY-01990 GFAP（Glial Fibrillary Acidic Protein）膠質(zhì)纖維酸性蛋白（抗原）              0.5mg/6501mg/1100                                  

QY-04240 MMP-1(matrix metalloproteinases-1)基質(zhì)金屬蛋白酶-1（抗原）                0.5mg/6501mg/1100                                  

QY-04120 MMP-2(Collagenase IV /Gelatinase A/Metallo proteinase-2)基質(zhì)金屬蛋白酶-2(多肽抗原)                   0.5mg/6501mg/1100                                    

QY-04130 MMP-3(matrix metalloproteinase-3/Transin-1/SL-1/Stromelysin-1 precursor)基質(zhì)金屬蛋白酶-3(抗原)                  0.5mg/6501mg/1100                                  

QY-04230 MMP-7(Matrilysin/matrix metalloproteinases-7)基質(zhì)金屬蛋白酶-7(抗原)            0.5mg/6501mg/1100                                  

QY-04140 MMP-14(Matrix metalloproteinase-14)金屬基質(zhì)蛋白酶-14                    0.5mg/6501mg/1100                                  

QY-02010 GFRα-1（GDNF family receptor alpha-1）GDNF家族受體α-1（抗原）            0.5mg/6501mg/1100                                  

QY-03510 GLUT2(Glucose transporter type 2）葡萄糖轉(zhuǎn)運蛋白2（抗原）                  0.5mg/6501mg/1100                                  

QY-01680 GFRA4 ( Persephin receptor ) (GFR receptor alpha 4)GDNF家族受體α-4（抗原）              0.5mg/6501mg/1100                                  

QY-02050 GHRF (GHRH)生長激素釋放激素（因子）（抗原）               0.5mg/6501mg/1100                                  

QY-08000 ANGPTL1/ANG-1 (Angiopoietin-like Protein 1)血管位蛋白樣1/血管生成素樣蛋白-1抗原                    0.5mg/6501mg/1100                                    

QY-03840 GLUT4(Glucose transporter type 4）葡萄糖轉(zhuǎn)運蛋白4（抗原）                  0.5mg/6501mg/1100                                   【人糖基化依賴的細胞黏附分子(GlyCAM-1)ELISA試劑盒廠家】

QY-00980 GIP （Gastric Inhibitory polypeptide）胃泌素抑制肽（抗原）           0.5mg/6501mg/1100                                  

QY-02080 GLP-2（Glucagon-like peptide-2）胰高血糖素樣肽-2（多肽抗原）            0.5mg/6501mg/1200                                  

QY-01940 GRP94 （gp96）94kDa 糖調(diào)節(jié)蛋白（抗原）              0.5mg/6501mg/1100                                  

QY-03480 G Protein(Guanine nucleotide-binding regulatory protein)G蛋白（鳥苷酸結(jié)合蛋白）（抗原）                  0.5mg/6501mg/1100                                  

QY-02520 GR (Glucocorticoid receptor)糖皮質(zhì)激素受體 多肽               0.5mg/6501mg/1100                                  

QY-00230 GSK-3β(NT) (Glycogen Synthase Kinase-3β)糖原合酶激酶-3β（抗原）           0.5mg/6501mg/1100                                  

QY-03410 CKR-L2（ G protein-coupled receptor-2 ）G蛋白偶聯(lián)受體-2(抗原)            0.5mg/6501mg/1100                                  

QY-00280 GSK-3β, (Glycogen Synthase Kinase-3)葡萄糖合成激酶-3（抗原）             0.5mg/6501mg/1100                                  

QY-01360 GTP-CH-1三磷酸鳥苷環(huán)水解酶（抗原）               0.5mg/6501mg/1100                                  

QY-03440 H5N1-M2（Avian influenza Matrix Protein-2）H5N1流感病毒M2型（抗原）                   0.5mg/6501mg/1100                                  

QY-02210 HCV-Core丙型肝炎病毒-C區(qū)（多肽）                    1mg/7002mg/1300                            

QY-02180 HCV-NS1丙型肝炎病毒-NS1（抗原）            0.5mg/6501mg/1100                                  

QY-02190 HCV-NS3丙型肝炎病毒-NS3（抗原）            0.5mg/10001mg/1800                               

QY-02130 HCV-NS4a丙型肝炎病毒-NS4a（抗原）                 0.5mg/10001mg/1800                               

QY-02120 HEV戊型肝炎病毒（抗原）                    0.5mg/6501mg/1100