【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【人胸腎表達(dá)趨化因子(BRAK/CXCL14)ELISA試劑盒廠家】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人胸腎表達(dá)趨化因子(BRAK/CXCL14)ELISA試劑盒廠家】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【人胸腎表達(dá)趨化因子(BRAK/CXCL14)ELISA試劑盒廠家】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人胸腎表達(dá)趨化因子(BRAK/CXCL14)ELISA試劑盒廠家】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

870001    KRAS RGQ PCR Kit (24)             CNY                           

805965    REPLI-g Service,  HT (500 µg)          CNY                           

800443    cDNA Library, Picked Clones              CNY                           

290075    mericon S. aureus Kit (96)           CNY                           

9012187  Zahnstange spez. Nadelantrieb P1xxx          CNY                            【人胸腎表達(dá)趨化因子(BRAK/CXCL14)ELISA試劑盒廠家】

9012032  Base plate, Z motor, handler, BR8000           CNY                           

9001673  PyroMark Q96 MD Instrument     For performing pyrosequncing reaction on the Q96 MD     CNY                           

9001659  Rotor-Gene Q 5plex HRM (w/o PC) PrioPLUS    Rotor-Gene Q 5plex HRM (w/o PC) PrioPLUS includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 3 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 3 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.      CNY                           

9001657  Rotor-Gene Q 5plex HRM (w/o PC) System       Rotor-Gene Q 5plex HRM (w/o PC) System includes the Rotor-Gene system ａnd installation. The Installation ａnd Introductory Training is an on-site service, conducted by a QIAGEN Service Specialist. It covers the handling of the Rotorgene-Q systems hardware ａnd trains the user on operation of the Rotorgene software. All travel ａnd labor cost included for Installation ａnd service documented on a GMP/GLP-compliant Field Service document.    CNY                           

950233    Binding Reagent (VXB) (350 ml)          CNY                           

37571     Qproteome O-Glycan Glycoprotein Kit        CNY                           

9015330  Sensor, photo, EE-SX670A          CNY                           

9015322  Belt, M-motor, M48              CNY                           

133026    NeXtalStock Calcium chloride  (200)         CNY                           

133025    NeXtalStock Calcium acetate  (200)           CNY                           

9001630  Rotor-Gene Q 2plex HRM System        CNY                           

249900    QuantiTect Primer Assay (200)     For 200 x 50 µl reactions: lyophilized mix of forward ａnd reverse primers for real-time analysis with SYBR Green detection.    CNY                           

133006    NeXtalStock MES sod. salt pH 6.5 (200)            CNY                           

9011965  Tip Adapter 300 µl BR8000          CNY                           

204554    QuantiFast Probe RT-PCR+ROXvial Kit(400)             CNY                           

133064    NeXtalStock Magnesium sulfate 6H2O (200)              CNY                            【人胸腎表達(dá)趨化因子(BRAK/CXCL14)ELISA試劑盒廠家】

870101    EGFR RGQ PCR Kit (24)             CNY                           

290023    mericon L. monocytogenes Kit (24)            CNY                           

204443    QuantiTect Probe RT-PCR Kit (200)     For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect Probe RT-PCR Master Mix,* 100 ul QuantiTect RT Mix, 2 x 2.0 ml RNase-free water       CNY                           

133052    NeXtalStock CTAB (200)             CNY                           

9018397  Transport Box, QIAxcel        CNY                           

9001650  Rotor-Gene Q 5plex HRM System        CNY                           

37612     Qproteome Mitochondria Isolation Kit          CNY                           

9015333  O-rings, pressure sensor fixing, M48 (2)            CNY                           

133032    NeXtalStock Citric acid  (200)           CNY                           

133023    NeXtalStock Cadmium chloride  (200)              CNY                           

9001638  Rotor-Gene Q 2plex HRM (w/o PC) Priority       Rotor-Gene Q 2plex HRM (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.      CNY                           

9001611  QIAgility HEPA / UV (incl.Laptop)        CNY                           

 QY-021029     CD49(非常晚期抗原)抗體   Anti-CD49/VLA(very late Anti-gens)                               

 QY-021030     CD5抗體       Anti-CD5                                   【人胸腎表達(dá)趨化因子(BRAK/CXCL14)ELISA試劑盒廠家】

 QY-021031     細(xì)胞間粘附分子-1抗體       Anti-CD54/ICAM-1(Intercellular dhesion molecule 1)                            

 QY-021032     細(xì)胞間粘附分子-2抗體       Anti-CD102/ICAM-2（intercellular adhesion molecules-2）                                 

 QY-021033     細(xì)胞間粘附分子-3抗體       Anti-CD50/ICAM-3（intercellular adhesion molecules-3）                            

 QY-021034     衰變加速因子CD55抗體    Anti-CD55/DAF                               

 QY-021035     反應(yīng)性溶血膜抑制蛋白抗體       Anti-CD59/potectin                                  

 QY-021036     神經(jīng)細(xì)胞粘附分子1抗體（Elisa IP IHC 用）  Anti-CD56/NCAM1(Neural Cell Adhesion Molecule 1)                                  

 QY-021037     神經(jīng)細(xì)胞粘附分子1抗體    Anti-CD56/NCAM1(Neural Cell Adhesion Molecule 1)                                  

 QY-021038     神經(jīng)細(xì)胞粘附分子配體1抗體  Anti-CD171/NCAM-L1（Neural cell adhesion molecule ligand 1）                               

 QY-021039     CD57抗體     Anti-CD57                                

 QY-021040     CD68抗體（用于大鼠、小鼠）  Anti-CD68（to Mouse, rat）                                  

 QY-021041     CD68抗體（人、牛）  Anti-CD68（to human,bocine）                              

 QY-021042     刺激分子B7-1蛋白抗體      Anti-CD80/B7-1                               

 QY-021043     轉(zhuǎn)鐵蛋白受體抗體       Anti-CD71/TFR(Transferrin Receptor)                                  

 QY-021044     胞漿-5´-核苷酸酶-Ⅱ抗體   Anti-CD73(5´-nucleotidase cytosolicⅡ)                                

 QY-021045     免疫球蛋白結(jié)合蛋白-1抗體       Anti-CD79A/IGBP-1(Immunoglobuin binding protein-1)                               

 QY-021046     腫瘤轉(zhuǎn)移抑制蛋白1抗體    Anti-CD82/KAI1                                【人胸腎表達(dá)趨化因子(BRAK/CXCL14)ELISA試劑盒廠家】

 QY-021047     鼠抗人CD8單克隆抗體      Anti-CD8（mouse monoclonal antibody）                             

 QY-021048     CD86抗體     Anti-CD86/B7-2                               

 QY-021049     周期素依賴激酶1抗體       Anti-Cdc2/P34CDC2/CDK1(Cell division control protein 2)                                  

 QY-021050     細(xì)胞毒性受體NK-p46抗體        Anti-CD335/NKp46/NCR1                               

 QY-021051     細(xì)胞毒性受體NK-p44抗體        Anti-CD336/NKp44/NCR2(Natural killer cell p44-related protein)                                 

 QY-021052     細(xì)胞毒性受體NK-p30抗體        Anti-CD337/NKp30/NCR3(Natural killer cell p30-related protein)                                 

 QY-021053     細(xì)胞分裂周期蛋白6抗體    Anti-Cdc6（Cell Division Cycle protein 6）                            

 QY-021054     抗Jagged1/CD339抗體        Anti-CD339/Jagged1                                

 QY-021055     細(xì)胞周期調(diào)控因子34   Anti-Cdc34（Cell Division Cycle protein 34）                               

 QY-021056     周期素依賴性激酶1抗體    Anti-CDK1 (Cyclin-Dependent Kinase 1)                                

 QY-021057     周期素依賴性激酶2抗體    Anti-CDK2 (Cyclin-Dependent Kinase 2)                                

 QY-021058     周期素依賴性激酶3抗體    Anti-CDK3 (cyclin-dependent kinase 3)                                  

 QY-021059     周期素依賴性激酶4抗體    Anti-CDK4 (cyclin-dependent kinase 4)