ELISA酶聯(lián)法試劑盒操作因固相載體的形成不同而有所差異，國內(nèi)醫(yī)學(xué)檢驗(yàn)一般均用板式點(diǎn)。本文將敘述板式ELISA各個操作步驟的注意要點(diǎn)，珠式、管式及磁性球ELSIA，國外試劑均與特殊儀器配合應(yīng)用，兩者均有詳細(xì)的使用說明，嚴(yán)格遵照規(guī)定操作，必能得出準(zhǔn)確的結(jié)果。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

首先齊一生物優(yōu)質(zhì)的試劑盒，良好的儀器和正確的操作是保證ELISA檢測結(jié)果準(zhǔn)確可靠必要條件。所以ELISA試劑盒的購買，試劑質(zhì)量的保證以及實(shí)驗(yàn)前實(shí)驗(yàn)儀器的檢察都是很有重要性的。保證實(shí)驗(yàn)材料的質(zhì)量之后要嚴(yán)格按照實(shí)驗(yàn)步驟進(jìn)行。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【重組人粒細(xì)胞集落刺激因子（rHG-CSF）試劑盒廠家】齊一生物ELISA試劑盒

1.精心挑選的進(jìn)口抗體、標(biāo)準(zhǔn)品、顯色劑和酶標(biāo)板全部為進(jìn)口產(chǎn)品實(shí)現(xiàn)*佳性能；

2.原材料批量進(jìn)口加上自配的特殊配方稀釋劑保證高品質(zhì)產(chǎn)品性能的同時兼顧成本；

3.采用技術(shù)，嚴(yán)格按照標(biāo)準(zhǔn)生產(chǎn)方案組裝生產(chǎn)；

4.預(yù)包被微孔板的批內(nèi)和批間變異系數(shù)均小于10 ％；

5.經(jīng)過嚴(yán)格交叉反應(yīng)和干擾測試及穩(wěn)定性試驗(yàn)；

6.明確的敏感度；

7.針對所有樣品類型達(dá)到*佳的性能；

8.廣泛的檢測范圍可以滿足大多數(shù)檢測要求；

9.完善的售后服務(wù)和免費(fèi)的技術(shù)支持免去您的后顧之憂；

10.強(qiáng)大的生產(chǎn)能力和充足的儲備、操作熟練的技術(shù)人員及高效的生產(chǎn)流程，隨時可以組裝出您定制的產(chǎn)品。

【重組人粒細(xì)胞集落刺激因子（rHG-CSF）試劑盒廠家】齊一生物僅供實(shí)驗(yàn)人、大鼠、小鼠、兔等動物實(shí)驗(yàn)標(biāo)本檢測使用！得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！齊一生物銷售：021－6034 8496；181214 53965；173021 04490

ELISA試劑盒問題描述

標(biāo)準(zhǔn)曲線梯度差：

吸液或加液不準(zhǔn)請檢查移液器及吸頭

標(biāo)準(zhǔn)品稀釋不正確溶解標(biāo)準(zhǔn)品稍微旋轉(zhuǎn)瓶身，輕輕混勻使粉末完全溶解

洗滌不完全保證洗滌時間和洗滌次數(shù)及每孔的加液量

顯色很弱或無色：

孵育時間太短保證充足的孵育時間

實(shí)驗(yàn)溫度不正確使用推薦的實(shí)驗(yàn)溫度

試劑體積不夠或漏加、稀釋不正確檢查吸液及加液過程，保證所有試劑按順序足量添加

讀數(shù)數(shù)值低：

酶標(biāo)儀設(shè)置不正確 在酶標(biāo)儀上檢查波長及濾光片設(shè)置

提前打開酶標(biāo)儀預(yù)熱：變異系數(shù)大加液不正確檢查加液情況

【重組人粒細(xì)胞集落刺激因子（rHG-CSF）試劑盒廠家】背景值高：

檢測抗體的工作濃度使用推薦的稀釋倍數(shù)

酶標(biāo)板洗滌不完全重新閱讀操作手冊，保證清洗完全；如果用自動洗板機(jī)，請檢查所有的出口是否有堵塞

洗液有污染配制新鮮的洗液

靈敏度低：

ELISA試劑盒保存按說明書要求保存相關(guān)試劑

讀數(shù)前未終止OD讀數(shù)前應(yīng)在每孔中加入終止液

齊一生物客戶寄標(biāo)本時需注明以下情況：

1、標(biāo)本編號；2、所測項(xiàng)目；3、是否做復(fù)孔；3、聯(lián)系方式；4、實(shí)驗(yàn)后標(biāo)本是否寄回。

客戶必知：

客戶應(yīng)對所提供的材料及信息負(fù)責(zé)，如因客戶提供的材料及信息不準(zhǔn)確而引起的實(shí)驗(yàn)延誤或經(jīng)濟(jì)損失由客戶承擔(dān)。

試劑盒樣本要求

在收集標(biāo)本前都必須有一個完整的計(jì)劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時儲存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請?jiān)炷Ｈ〔暮�，將�?biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。具體操作注意事項(xiàng)請與我司技術(shù)人員溝通。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【重組人粒細(xì)胞集落刺激因子（rHG-CSF）試劑盒廠家】液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

1. 血清：室溫血液自然凝固10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。

2. 血漿：應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑，混合10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成應(yīng)再次離心。

3. 尿液、胸腹水、腦脊液：用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。如有沉淀形成，應(yīng)再次離心。

4. 細(xì)胞培養(yǎng)上清：檢測分泌性的成份時，用無菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。

5. 培養(yǎng)細(xì)胞:檢測細(xì)胞內(nèi)的成份時，用PBS（PH7.2-7.4）稀釋細(xì)胞懸液，細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融或加入組織蛋白萃取試劑，以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。

6. 組織標(biāo)本：切割標(biāo)本后，稱取重量。加入一定量的PBS，緩沖液中可加入1μg/L蛋白酶抑制劑或50U/ml的Aprotinin（抑肽酶）。用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清置于-20度或-70度保存，如有必要，可以將樣品濃縮干燥。分裝后一份待檢測，其余冷凍備用。

7. 標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn)，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融

8.不能檢測含NaN3的樣品，因NaN3抑制辣根過氧化物酶的（HRP）活性。

9.如果樣品不能立即檢測，應(yīng)將其分裝，-70 ℃保存，避免反復(fù)冷凍。保存過程中如出現(xiàn)沉淀，應(yīng)再次離心。血液標(biāo)本盡可能的不要使用溶血或高血脂血。如果血清中含大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解

凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

37601     Qproteome Plasma Membrane Protein Kit           CNY                           

9015329  O-rings, Norpren tube fixing, M48 (2)         CNY                           

9015328  Seal, cap, M48 (6)         CNY                           

239095    GelPilot 1 kb Plus Ladder (100)    Ready-to-use DNA marker, 600µl (100lanes), 12 fragments: 100 – 10000 bp    CNY                           

133127    NeXtalStock Sodium citrate (200)        CNY                            【重組人粒細(xì)胞集落刺激因子（rHG-CSF）試劑盒廠家】

133126    NeXtalStock TRIS-HCl pH 8.5 (200)           CNY                           

133008    NeXtalStock 2-Propanol (200)             CNY                           

133007    NeXtalStock MES sod. salt untit. (200)              CNY                           

9011949  Board, X-main              CNY                           

9011945  Tube Holder, STS, 11ml (6)   Holder for accommodating 8 tubes (11 ml) in the sample tracking system; pack of 6       CNY                           

9243588  RCS, Premium Agreement     "- Service response time RCS: 24 Hrs

- Inspection/maintenance delivery: yes, included; 1 /agreement year

- Cost coverage for repair parts: yes, included

- Cost coverage for labor: yes, included

- Cost coverage for travel: yes, included

- Replacement system provision for DML: yes, included

- Transportation cost coverage for DML: yes, included

For defect units, after customer calling QIAGEN technical service hotline before 9 am:

RCS:

- QIAGEN shall dispatch a specialist to repair the defect instrument at customer site within 24 Hrs.

DML:

- A special package containing a replacement unit shall be sent to the customer within 48 Hrs / 2 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

Note: for DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement

The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP"  CNY                           

37013     Albumin Affinity Cartridges (3 x 1ml)          CNY                           

36910     Single-Tube Magnet       Magnet for separating magnetic particles in a 1.5 ml or 2 ml tube      CNY                           

36111      Ni-NTA Magnetic Agarose Beads (2 x 1 ml) 2 x 1 ml nickel-charged magnetic agarose beads (5% suspension)     CNY                           

35330     Penta-His Alexa Fluor 532 Conjugate    125 ul Penta-His Alexa Fluor 532 Conjugate, 200 ug/ml      CNY                           

218432    Mouse miScript Assay 384 Set VXX.X (20)        CNY                           

218412    Mouse miScript Primer Assay Set VXX.X           CNY                           

218380    #miScript PCR Control Set    miScript PCR Control Set allows customers to identify easily the right normalization control for their real-time PCR set up while working with miRNAs or other small RNAs. It includes 6 normalization control assays (for 50 real-time PCR rxns a 50µl) which are all designed against snoRNAs or snRNAs. Works for human mouse ａnd rat.       CNY                           

218193    miScript PCR Starter Kit (80)              CNY                           

9018294  EZ1 Advanced DNA Buffy Coat Card          CNY                           

800356    Handling Fee          CNY                            【重組人粒細(xì)胞集落刺激因子（rHG-CSF）試劑盒廠家】

9015298  Nozzles, M48 (6)          CNY                           

9243775  QIAsymphony AS Custom Protocol Advanced2          CNY                           

9019447  Sensor brd 4 CH temp sensor              CNY                           

9019444  Electrolytic capacitor 8000uF x 63V            CNY                           

9018158  Waste station, waste drawer, Qsym             CNY                           

9018154  Bottle, waste drawer, Qsym          CNY                           

9018135  Sensor, xz, et/hs, Qsym        CNY                           

9018132  Splash guard, extractor, Qsym             CNY                           

9017720  Appl. Pack Investigator for Univ.PF            CNY                           

9017832  Sensor, tip check, QIAC              CNY                           

9017830  Power entry port, QIAC        CNY                           

9017828  Power supply, 230 V, QIAC          CNY                           

QY-15892       Anti-MRP3(Multidrug Resistanec-Associated Protein 3)多藥耐藥相關(guān)蛋白3抗體                                    

QY-15902       Anti-MrpL28 (mitochondrial ribosomal protein L28)線粒體核糖體蛋白L28抗體                                       【重組人粒細(xì)胞集落刺激因子（rHG-CSF）試劑盒廠家】

QY-15912       Anti-MSH-2 (MutS homolog 2)錯配修復(fù)蛋白2抗體                                   

QY-15922       anti-MLH1(Mutl homolog l gene)錯配修復(fù)蛋白1抗體                                        

QY-15932       Anti-MSLN (mesothelin)間皮素抗體                                      

QY-15942       anti-MUC5AC/Mucin 5AC(Gastric Mucin M1)胃粘液素抗體                                      

QY-15952       Anti-MTR-1A (Melatonin receptor-1A)褪黑素受體/松果體素受體抗體                                      

QY-15962       Anti-mucin-1/Muc-1/CD227 antigen (Epithelial Membrane Antigen )粘蛋白-1/上皮膜抗原抗體                                       

QY-15972       Anti-MyD88 (myeloid differential protein-88)髓樣分化蛋白抗體                                        

QY-15982       Anti-Myelin P0 protein( peripheral myelin prothein Zero;MPZ;MPP)外周髓磷脂P0蛋白/P0蛋白抗體                                    

QY-15992       Anti-Myosin (Smooth Muscle)鼠抗人心肌肌凝蛋白(平滑肌) 單抗                                    

QY-16002       Anti-N-AChR α4 (Nicotinic-Acetylcholine receptor α4)煙堿型乙酰膽堿受體α4抗體                                        

QY-16012       Anti-N-AChR α7 (Nicotinic-Acetylcholine receptor α7)煙堿型乙酰膽堿受體α7抗體                                        

QY-16022       Anti-Nanog胚胎干細(xì)胞關(guān)鍵蛋白抗體                                   

QY-16032       anti-Natrexone抗納曲酮抗體IgG                                         

QY-16042       Anti-NAP1 (nucleosome assembly protein 1)核小體組裝蛋白1抗體                                         

QY-16052       Anti-N-cadherinN-鈣粘附分子抗體                                       

QY-16062       Anti-N-coR1 （Nuclear receptor co-repressor 1）核受體輔助抑制因子抗體                                    【重組人粒細(xì)胞集落刺激因子（rHG-CSF）試劑盒廠家】

QY-16072       Anti-Nephrin Protein腎病蛋白抗體                                       

QY-16082       Anti-Nestin巢蛋白/神經(jīng)上皮干細(xì)胞蛋白抗體                                     

QY-16092       Anti-Nestin巢蛋白/神經(jīng)上皮干細(xì)胞蛋白抗體                                     

QY-16102       Anti-Neurobeachin protein (AKAP550)蛋白激酶錨定蛋白/激酶固定蛋白抗體                                         

QY-16112       Anti-Neurocan神經(jīng)粘蛋白抗體                                     

QY-16122       Anti-Neurofascin-155神經(jīng)束蛋白-155                                   

QY-16132       Anti-NF-H（Neurofilament triplet H)高分子量神經(jīng)絲蛋白抗體                                   

QY-16142       Anti-NFKBp65(p65 NF-kappa B;p65NFKB)細(xì)胞核因子/k基因結(jié)合核因子抗體                                       

QY-16152       Anti-NF-L（Neurofilament triplet L)低分子量神經(jīng)絲蛋白抗體                                   

QY-16162       Anti-NF-M (Neurofilament triplet M）中分子量神經(jīng)絲蛋白抗體                                       

QY-16172       Anti-NF-κBp50（p50 NF-kappa B;p50NFKB）細(xì)胞核因子50/κ基因結(jié)合核因子50抗體                                    

QY-16182       Anti-NGF-R/p75NTR/CD271（p75 Neurotrophin R）神經(jīng)生長因子受體抗體                                         

QY-16192       Anti-NGF-β神經(jīng)生長因子-β抗體