檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【人白細(xì)胞介素-1β（IL-1β）ELISA試劑盒廠家】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【人白細(xì)胞介素-1β（IL-1β）ELISA試劑盒廠家】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【人白細(xì)胞介素-1β（IL-1β）ELISA試劑盒廠家】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn).

【人白細(xì)胞介素-1β（IL-1β）ELISA試劑盒廠家】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見(jiàn)應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

331451     miScript PreAMP PCR Kit (12)           CNY                             

1070498  Buffer TCL, 2x (8.5 ml),G          CNY                             

1070211  Cramp (1)                   CNY                             

981313     Rotor-Disc 100 (300)                  CNY                             

981008     PCR Tubes, 0.2 ml  (10000)               CNY                             

9012945  Liquid Handling Kit Plato 3000 (500ul)              CNY                             

9012821  Liquid Handling Kit Plato 3301 (1000ul)            CNY                             

133257     NeXtalStock HEPES sod. salt pH 7.6 (200)                 CNY                             

330612     RT² SYBR Green Fluor FAST Mastermix (12)            CNY                              【人白細(xì)胞介素-1β（IL-1β）ELISA試劑盒廠家】

330521     RT² SYBR Green ROX qPCR Mastermix (8)                CNY                             

59665       EpiTect Control DNA, unmethylated (100)                CNY                             

9010046  Modul, Incubator, 6 MTP, P3xxx                CNY                             

9010024  Incubator/stacker 4/4 plates Plato 1/3               CNY                             

206544     Type-it HRM PCR Kit (400)                 CNY                             

206243     Type-it Microsatellite PCR Kit (200)          "The Type-it Microsatellite PCR Kit is developed for fast ａnd reliable genotypic analysis of humans, animals, plants, ａnd bacteria using

microsatellites, STR, or VNTR markers without the need for lengthy optimization procedures

Features

 Reliable microsatellite analysis by multiplex PCR

 Microsatellite assay development without optimization

 Successful ａnd specific coamplification of all fragments

 Optimized protocols for any detection platform"       CNY                             

9012737  Syringe pump outlet tubing (4.7m)           CNY                             

9012720  Set of Pipet Tubing Plato 3001                   CNY                             

9012717  Troughs, 50ml (100 troughs + lids)            CNY                             

133237     NeXtalStock Sodium citrate pH 5.4 (200)                  CNY                             

133236     NeXtalStock Sodium citrate pH 5.2 (200)                  CNY                             

9002193  QIAcube (230V), PrioPLUS, no PM   "QIAcube (230V) Instrument

Installation of QIAcube  instrument & Introductory training

3 years coverage on parts, labor ａnd travel

On site service, 48 hr response

No Preventive Maintenance

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service"        CNY                             

9002033  Rotor-Gene Q MDx 5plex HRM System            CNY                             

59205       EpiTect Whole Bisulfitome Kit (100)                  CNY                             

59124       EpiTect Plus DNA Bisulfite Kit (48)            CNY                             

929551     QX DNA Size Marker FX174/HaeIII (50 µl)                 CNY                              【人白細(xì)胞介素-1β（IL-1β）ELISA試劑盒廠家】

929528     QX Alignment Marker 50 bp/3 kb (1.5 ml)                 CNY                             

972807     PyroMark Gold Q96 Reagents (50 x 96)  For performing pyrosequncing reaction on the Q96 MD          CNY                             

972442     PyroMark Q96 SNP HFE (96)    For detection of variants in HFE gene      CNY                             

133221     NeXtalStock Sodium ac. H2O pH 5.6 (200)                CNY                             

133220     NeXtalStock Sodium ac. H2O pH 5.0(200)                 CNY                             

301704     HiPerFect Transfection Reagent (0,5 ml)                   CNY                             

301427     Effectene Transfection Reagent (4x1ml) 4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates      CNY                             

51326       QIAamp DNA Mini QIAcube Kit (240)                CNY                             

QT-00185 小腸結(jié)腸炎耶爾森氏菌ATCC23715      凍干粉                                         

QT-00186 亞利桑那沙門(mén)氏菌CMCC(B)47001        凍干粉                                         

QT-00187 熱帶念珠菌ATCC13803 凍干粉                                         

QT-00188 克柔假絲酵母菌ATCC14243 凍干粉                                         

QT-00189 光滑念珠菌ATCC15126 凍干粉                                         

QT-00190 創(chuàng)傷弧菌ATCC27562      凍干粉                                         

QT-00191 嬰兒雙歧桿菌ATCC15697      凍干粉                                         

QT-00192 德式乳桿菌保加利亞亞種CICC6032     凍干粉                                         

QT-00193 生孢梭菌CMCC（B）64941  凍干粉                                         

QT-00194 嗜熱鏈球菌CICC6038     凍干粉                                         

QT-00195 SphingobacteriumcompostiDSM18850   凍干粉                                         

QT-00196 豬霍亂沙門(mén)氏菌豬霍亂亞種DSM4224         凍干粉                                         

QT-00197 沼澤紅假單胞菌DSM5859    凍干粉                                         

QT-00198 嗜鹽細(xì)菌屬DSMZ16465         凍干粉                                          【人白細(xì)胞介素-1β（IL-1β）ELISA試劑盒廠家】

QT-00199 土壤伯克氏菌DSMZ18235    凍干粉                                         

QT-00200 煙酸芽孢桿菌DSMZ2923      凍干粉                                         

QT-00201 菲律賓鏈霉菌DSMZ40112=ATCC23905         凍干粉                                         

QT-00202 抗輻射鏈霉菌DSMZ41901    凍干粉                                         

QT-00203 短乳桿菌DSMZ6235      凍干粉                                         

QT-00204 乳酸片球菌JCM8791=DSM20284=NCIMB12174  凍干粉                                         

QT-00205 桃色歐文氏菌NBRC102418  凍干粉                                         

QT-00206 白色噬瓊膠菌NBRC102603  凍干粉                                         

QT-00207 浮游球衣菌NBRC13543         凍干粉                                         

QT-00208 丁香假單胞菌NBRC14078     凍干粉                                         

QT-00209 睪丸酮叢毛單胞菌NBRC14951     凍干粉                                         

QT-00210 耐放射異常球菌NBRC15346         凍干粉                                         

QT-00211 煙酸芽孢桿菌NBRC15566     凍干粉                                         

QT-00212 新鞘氨醇單胞菌NBRC16725         凍干粉                                         

QT-00213 假交替單胞菌NCCB100134   凍干粉                                         

QT-00214 腐敗希瓦氏菌NCIMB12202  凍干粉                                          【人白細(xì)胞介素-1β（IL-1β）ELISA試劑盒廠家】

QT-00215 食油假單胞菌NCIMB6576    凍干粉