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齊一生物現(xiàn)貨供應(yīng)人ELISA試劑盒，大鼠ELISA試劑盒，小鼠ELISA試劑盒等現(xiàn)貨充足，購買任意ELISA試劑盒都享受免費代測服務(wù)，更多進口原裝品牌試劑盒供應(yīng)齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人白細胞介素-10（IL-10）ELISA試劑盒現(xiàn)貨】酶法檢測試驗所需自備物品:

1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【人白細胞介素-10（IL-10）ELISA試劑盒現(xiàn)貨】操作注意事項：

1.試劑盒應(yīng)按標簽說明書儲存，使用前恢復到室溫30分鐘后方可使用。稀稀過后的標準品應(yīng)丟棄，不可保存。

2.實驗中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號的試劑不要混用.請在試劑盒保質(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標板時應(yīng)充分拍干，不要將吸水紙直接放入酶標反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長時間打開蓋子。底物B對光敏感，避免長時間暴露于光下。避免用手接觸，有毒。實驗完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時間一樣。

9.按照說明書中標明的時間、加液的量及順序進行溫育操作。

【人白細胞介素-10（IL-10）ELISA試劑盒現(xiàn)貨】齊一生物客戶寄標本時需注明以下情況：

1、標本編號；2、所測項目；3、是否做復孔；3、聯(lián)系方式；4、實驗后標本是否寄回。

客戶必知：

客戶應(yīng)對所提供的材料及信息負責，如因客戶提供的材料及信息不準確而引起的實驗延誤或經(jīng)濟損失由客戶承擔。

齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【人白細胞介素-10（IL-10）ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)，以上資料供參考如有疑問需要詳細資料請聯(lián)系我們客服人員，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

59445       EpiTect HRM PCR Kit (100)                CNY                             

381215     Investigator HDplex Kit (100)           CNY                             

4863004  pg NDV Univ. RT-PCR ASSAY LE                  CNY                             

4873004  pg AIV-H5 RT-PCR ASSAY           CNY                             

130923     NeXtal DWBlock Classics II Suite               CNY                             

130920     NeXtal DWBlock JCSG+ Suite            CNY                             

4621063  artus MTHFR LC PCR Kit (24) CE                CNY                             

4841014  pg HPV PCR ASSAY            CNY                             

4555265  artus M.tuberculosis RG PCR Kit (96) CE          CNY                             

4551065  artus Borrelia LC PCR Kit (96) CE               CNY                             

4513303  artus HI Virus-1 QS-RGQ Kit (24) RUO              CNY                             

4510033  artus Enterovirus LC RT-PCR Kit (24) PRE                   CNY                              【人白細胞介素-10（IL-10）ELISA試劑盒現(xiàn)貨】

130718     NeXtal Tubes PACT Suite           CNY                             

122001     SeqTarget Primer Select (400)                   CNY                             

4506165  artus HBV TM PCR Kit (96) CE          CNY                             

120016     QIAquick 96 Plates (24)    Individual QIAquick 96 plates ａnd handbook. No buffers or reagents.    CNY                             

75162       RNeasy Maxi Kit (12)        12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents ａnd Buffers  CNY                             

74704       RNeasy Fibrous Tissue Mini Kit (50)         50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), Proteinase K, RNase-free DNase I, RNase-free Reagents ａnd Buffers  CNY                             

27406       QuickLyse Miniprep Kit (250)            CNY                             

73504       RNeasy FFPE Kit (50)                  CNY                             

73442       RNeasy Plus Universal Tissue Midi Kit10          CNY                             

72041       Oligotex Direct mRNA Midi/Maxi Kit       For 6 mRNA midi or 2 maxipreps: 1 ml Oligotex Suspension, Large Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents ａnd Buffers  CNY                             

26173       R.E.A.L. Prep 96 Plasmid Kit (24)     For 24 x 96 rapid extraction alkaline lysis minipreps: 24 QIAfilter 96 Plates, Square-Well Blocks, Tape Pads, Reagents, Buffers      CNY                             

20051       QIAEX II Gel Extraction Kit (500)     For 500 extractions: 5 x 1.0 ml QIAEX II Suspension, Buffers  CNY                             

19743       QIAfilter Midi Cartridges (25)  25 QIAfilter Midi Cartridges     CNY                             

69982       TissueLyser Adapter Set (2 x 24)      2 sets of Adapter Plates ａnd 2 racks for use with 2.0 ml microcentrifuge tubes on the TissueLyser. Also contains 6 silicone sealing strips. (3 per adapter) to ensure 2-ml tubes are secure.    CNY                             

19413       QIAvac 24 Plus          CNY                             

19088       Reagent DX               CNY                              【人白細胞介素-10（IL-10）ELISA試劑盒現(xiàn)貨】

19086       Buffer EB (250ml)    250 ml Elution Buffer       CNY                             

19073       Buffer AVL        "Buffer AVL (5x 31 ml, 250 preps)

(""QIAamp Viral Lysis Buffer, includes 5x 310 µg Carrier RNA (26.6 ug/ml), 270 Mini preps"")"    CNY                             

19051       Buffer P1 500 ml Resuspension Buffer (RNase A not included)        CNY                             

16181       QIAGEN Plasmid Plus 96 Miniprep  Kit (4)               CNY                             

12462       QIAGEN Large-Construct Kit (10)    10 QIAGEN-tip 500, Reagents, Buffers, ATP-Dependent Exonuclease    CNY                            

QT-00076 糞腸球菌(高耐)ATCC51299    凍干粉                                         

QT-00078 阪崎腸桿菌ATCC51329 凍干粉                                         

QT-00080 克柔念珠菌ATCC6258    凍干粉                                         

QT-00082 金黃色葡萄球菌ATCC6538    凍干粉                                         

QT-00084 枯草芽孢桿菌ATCC6633        凍干粉                                         

QT-00086 肺炎克雷伯菌ATCC700603    凍干粉                                         

QT-00088 熱帶念珠菌ATCC750      凍干粉                                         

QT-00090 嗜熱脂肪地芽孢桿菌ATCC7953    凍干粉                                         

QT-00092 植物乳桿菌ATCC8014    凍干粉                                         

QT-00094 大腸埃希氏菌ATCC8739        凍干粉                                         

QT-00096 白色假絲酵母/白色念球菌ATCC90029         凍干粉                                          【人白細胞介素-10（IL-10）ELISA試劑盒現(xiàn)貨】

QT-00100 銅綠假單胞桿菌（綠膿桿菌）ATCC9027     凍干粉                                         

QT-00102 枯草芽孢桿菌黑色變種ATCC9372        凍干粉                                         

QT-00103 艱難梭菌ClostridiumdifficileATCC9689  凍干粉                                         

QT-00104 啤酒酵母ATCC9763        凍干粉                                         

QT-00105 黑曲霉ATCC10582 凍干粉                                         

QT-00106 磚紅鏈霉菌ATCC19776 凍干粉                                         

QT-00107 白色葡萄球菌ATCC8799        凍干粉                                         

QT-00108 皺褶青霉ATCC10128      凍干粉                                         

QT-00109 黑曲霉ATCC10864 凍干粉                                         

QT-00110 釀酒酵母ATCC26603      凍干粉                                         

QT-00111 釀酒酵母ATCC26785      凍干粉                                         

QT-00112 運動發(fā)酵單孢菌ATCC29191 凍干粉                                         

QT-00113 假單胞桿菌ATCC31554 凍干粉                                         

QT-00114 糞產(chǎn)堿桿菌ATCC31555 凍干粉                                         

QT-00115 菅囊酵母ATCC32691      凍干粉                                         

QT-00116 弗氏檸檬酸桿菌ATCC10787 凍干粉                                          【人白細胞介素-10（IL-10）ELISA試劑盒現(xiàn)貨】

QT-00117 莢膜紅細菌ATCC11166 凍干粉                                         

QT-00118 發(fā)根土壤桿菌（發(fā)根植物單胞菌）ATCC11325  凍干粉                                         

QT-00119 生孢梭菌（產(chǎn)芽孢梭狀芽孢桿菌）ATCC11437  凍干粉                                         

QT-00120 痤瘡丙酸桿菌ATCC11827      凍干粉                                         

　　測定小分子量物質(zhì)常用競爭法，其標準曲線中吸光度與受檢物質(zhì)的濃度呈負相關(guān)。標準曲線的形狀因試劑盒所用模式的差別而略有不同。ELISA測定的標準曲線注意圖中橫坐標為對數(shù)關(guān)系，這更有利于測定系統(tǒng)的表達。

        講述完了以上ELISA實驗的操作要點，可以發(fā)現(xiàn)其實很多步驟都并不復雜，重要的是操作的時候一定要注重細節(jié)，熟能生巧，才可以掌握ELISA實驗的精髓所在。

133269     NeXtalStock Phosphate-citr. pH 4.2 (200)                 CNY                             

990522     Filter-Tips, 200 µl (960)            CNY                             

990396     QIAcube Modified Protocol               CNY                             

4501263  artus EBV RG PCR Kit (24) CE            CNY                             

4501205  artus EBV RG PCR Kit (96) RUO                 CNY                             

4500265  artus HSV-1/2 RG PCR Kit (96) CE               CNY                             

949008     Autopure Precipitation Soln (3800 ml)              CNY                             

335001     EpiTect Methyl qPCR Assay (200)              CNY                             

990332     Filter-Tips, 200 µl (1024)          CNY                             

939011     Buffer ATL ( 4 x 50 ml)               CNY                             

9010929  Motor, Y, with gearbox encoder                CNY                             

9010855  ZLV BGR DT-Abstreifer 4-fach Plato 7...D                  CNY                             

133246     NeXtalStock Sodium phosphate pH9.2 (200)            CNY                              【人細胞角蛋白18-M65（CK18-M65）ELISA試劑盒廠家】

330601     RT² SYBR Green FAST Mastermix (8)                 CNY                             

929604     QX Wash Buffer (40 ml)             CNY                             

9010810  Source rack (96 pos) for tubes 16mm x 1                   CNY                             

9010636  Microplate station, tall    Station for accommodating a 96-well plate (or other labware with the same base dimensions) on the worktable  CNY                             

69581       DNeasy 96 Blood & Tissue Kit (4)    For 4 x 96 DNA minipreps: 4 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers       CNY                             

63206       DyeEx 2.0 Spin Kit (250)  250 DyeEx Spin Columns, Collection Tubes (2 ml)    CNY                             

207043     QuantiTect Whole Transcriptome Kit (25)                 CNY                             

9010253  Gripper, right, landoplasted, MTP 120             CNY                             

979101     PyroMark Q96 HS Plate (100)  For sample analysis of DNA template on MD. CNY                             

979009     PyroMark Annealing Buffer (250 ml)        For annealing of sequencing primer to DNA template.   CNY                             

62124       EZ1 DSP DNA Blood Kit (48)     CE-IVD Certified Kit for Extraction of DNA from human whole Blood. To be used in combination with EZ1 DSP Instrument (SAP 9001360) ａnd EZ1 DSP Blood Card (SAP 9017713). Available Input Volumes 350 µl, selectable elution volumes 200 µl. Processing time for 6 samples 25 Minutes.       CNY                             

301709     HiPerFect Transfection Reagent (100 ml)                  CNY                             

9002199  RGQ 6plex, PC, PrioPLUS, no PM     "RGQ 6plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

Loaner available in 2 days*

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

9002196  RGQ 2plex HRM, PC, PrioPLUS, basic,no PM   "RGQ 2plex HRM, PC Instrument

Installation of RGQ 2plex HRM & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Preventive Maintenance (RRC,  1 PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"          CNY                             

206546     Type-it HRM PCR Kit (2000)              CNY                             

206541     Rotor-Gene Type-it HRM Discovery Kit            CNY                              【人細胞角蛋白18-M65（CK18-M65）ELISA試劑盒廠家】

9012731  Diluent Trough + lid PK 80 white               CNY                             

9012721  syringe pump inlet tubing Plato 3100                CNY                             

978803     PyroMark OneStep RT-PCR Kit (200)                 CNY                             

133231     NeXtalStock Sodium cacodyl. pH 6.8 (100)                CNY                             

QT-00020 生孢梭菌ATCC19404      凍干粉                                         

QT-00022 破傷風桿菌/梭菌ClostridiumtetaniATCC19406     凍干粉                                         

QT-00024 化膿性鏈球菌ATCC19615      凍干粉                                         

QT-00025 黃桿菌屬ATCC21047      凍干粉                                         

QT-00026 近平滑念珠菌ATCC22019      凍干粉                                         

QT-00027 變異鏈球菌ATCC25175 凍干粉                                         

QT-00028 脆弱擬桿菌ATCC25285 凍干粉                                         

QT-00029 洋蔥假單胞菌ATCC25416      凍干粉                                         

QT-00030 大腸埃希氏菌ATCC25922      凍干粉                                         

QT-00032 金黃色葡萄球菌ATCC25923 凍干粉                                          【人細胞角蛋白18-M65（CK18-M65）ELISA試劑盒廠家】

QT-00034 宋內(nèi)氏志賀氏菌(Sh.sonnei)ATCC25931 凍干粉                                         

QT-00035 金黃色葡萄球菌ATCC27217 凍干粉                                         

QT-00037 創(chuàng)傷弧菌ATCC27562      凍干粉                                         

QT-00038 銅綠假單胞桿菌（綠膿桿菌）ATCC27853  凍干粉                                         

QT-00040 糞腸球菌ATCC29212      凍干粉                                         

QT-00041 金黃色葡萄球菌ATCC29213 凍干粉                                         

QT-00047 兩歧雙歧桿菌ATCC29521      凍干粉                                         

QT-00049 多形擬桿菌ATCC29741 凍干粉                                         

QT-00051 糞腸球菌ATCC33186      凍干粉                                         

QT-00053 空腸彎曲菌ATCC33291 凍干粉                                         

QT-00055 金黃色葡萄球菌ATCC33591 凍干粉                                         

QT-00057 大腸埃希氏菌ATCC35218      凍干粉                                         

QT-00059 屎腸球菌ATCC35667      凍干粉                                         

QT-00061 變異鏈球菌ATCC35668 凍干粉                                         

QT-00063 遲緩真桿菌/遲緩埃格特菌ATCC43055         凍干粉                                         

QT-00065 金黃色葡萄球菌ATCC43300 凍干粉                                          【人細胞角蛋白18-M65（CK18-M65）ELISA試劑盒廠家】

QT-00067 弗氏檸檬酸桿菌ATCC43864 凍干粉                                         

QT-00069 淋病柰瑟氏菌ATCC49226      凍干粉                                         

QT-00071 流感嗜血桿菌ATCC49247      凍干粉                                         

QT-00072 肺炎鏈球菌ATCC49619 凍干粉                                         

QT-00074 流感嗜血桿菌ATCC49766      凍干粉