1.購買我公司任意ELISA試劑盒指標(biāo)享受免費(fèi)代測(cè)，并提供技術(shù)指導(dǎo)服務(wù)！

二、實(shí)驗(yàn)材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時(shí)備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人輪狀病毒抗原(RVAg)ELISA試劑盒現(xiàn)貨】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請(qǐng)勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個(gè)復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實(shí)驗(yàn)者，注意低溫保存

5.準(zhǔn)備好所有實(shí)驗(yàn)額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測(cè)標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲(chǔ)。 【人輪狀病毒抗原(RVAg)ELISA試劑盒現(xiàn)貨】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測(cè)稀釋液，可能在設(shè)計(jì)時(shí)就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時(shí)間和溫度。

10.切勿使用不同生產(chǎn)批號(hào)的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時(shí)，避免泡沫產(chǎn)生。

12.在實(shí)驗(yàn)開始之前，安排好實(shí)驗(yàn)流程。在實(shí)驗(yàn)開始之前，清潔工作臺(tái)。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【人輪狀病毒抗原(RVAg)ELISA試劑盒現(xiàn)貨】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測(cè)的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測(cè)，對(duì)收集后當(dāng)天就進(jìn)行檢測(cè)的標(biāo)本，及時(shí)儲(chǔ)存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本，請(qǐng)?jiān)炷Ｈ〔暮螅瑢?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測(cè)標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項(xiàng)請(qǐng)與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請(qǐng)?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測(cè)指標(biāo)及其它特殊要求。

【人輪狀病毒抗原(RVAg)ELISA試劑盒現(xiàn)貨】五、代測(cè)數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實(shí)驗(yàn)具體詳細(xì)步驟；

3、完整的實(shí)驗(yàn)報(bào)告（試劑耗材，實(shí)驗(yàn)方法，實(shí)驗(yàn)結(jié)果及結(jié)果說明）；

4、實(shí)驗(yàn)進(jìn)行階段，我公司客服人員會(huì)及時(shí)向您匯報(bào)實(shí)驗(yàn)進(jìn)程。

762431     PAXgene Blood RNA MDx Kit (4)               CNY                             

203745     QIAGEN Fast Cycling PCR Kit (1000)        For 1000 x 20 µl reactions: 10 x 1 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution       CNY                             

203609     HotStarTaq Plus DNA Polymerase (25000)       100 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 100 x 2.0ml Q-Solution, 25 mM MgCl2         CNY                             

201205     Taq DNA Polymerase (1000)    "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180850     GeneRead qPCR FAST SYBR Green/ROX MM (2)               CNY                             

159300     QIAcard FTA Purification Reagent (500ml)                CNY                             

158928     Lytic Enzyme Solution (650 µl)          CNY                             

9240549  Imbalance PCB, Eppendorf, AP196/98               CNY                             

9240547  Motor, 230V, Eppendorf, AP196/98           CNY                             

9240528  Cable, Motor, Axis #5, TR, AP98                CNY                             

9240047  Thermostate unit, 120V, 1.04          CNY                             

9020029  Cable RC-3034, RGQ                  CNY                             

9020017  Oil for PM, EZ1                   CNY                             

9020016  Cable harness, VacuumStation PCB, CX1          CNY                             

9019938  Opto switch, P-axis, 1 ch. , Cx2                  CNY                             

9019486  HEPA filter, replacement cartridge, Cxx           CNY                              【人輪狀病毒抗原(RVAg)ELISA試劑盒現(xiàn)貨】

9238861  BR 8000, Premium Agreement        The Premium Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                             

9238855  BR MDx, Basic Agreement      The Basic Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238850  EZ1, Basic Agreement     The Basic Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.      CNY                             

9241226  Barcode Reader, EZ1a              CNY                             

9238812  Rectangular carrier, undrilled          CNY                             

9019467  PLATE GLASS SHORT SET GS              CNY                             

9019465  Pads, D=10mm, white (85), Cxx                CNY                             

9019459  Transformer (115V), HS            CNY                             

9233429  Valve, magnet, dispenser, BR8000           CNY                             

9233334  Solenoid Valve, 5-fold                CNY                             

9237332  Install, BR plus 2 integr. w. scheduling     Installation ａnd training, BioRobot System including two approved integration devices with scheduling   CNY                             

9237167  Bottom Air Filter               CNY                             

9232994  Board, LED, BR8000                  CNY                             

9237158  RTD Adapter Board XYP           CNY                             

9237152  Power Inlet                CNY                             

9237108  Protocol Dev./Opt., on-site (4-days)         The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 4 days by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.          CNY                              【人輪狀病毒抗原(RVAg)ELISA試劑盒現(xiàn)貨】

9237098  Upgrade QIAsoft, on-site                   CNY                             

OPCM       少突膠質(zhì)前體細(xì)胞培養(yǎng)基  Oligodendrocyte Precursor Cell Medium          500 ml                                 

OsM 球狀少突細(xì)胞培養(yǎng)基  Oligosphere Medium        500 ml                                 

KM   角質(zhì)細(xì)胞培養(yǎng)基  Keratinocyte Medium      500 ml                                 

KM-d         角質(zhì)細(xì)胞培養(yǎng)基（確定）          Keratinocyte Medium-defined         500 ml                                 

FM-sf        成纖維細(xì)胞培養(yǎng)基         Fibroblast Medium-serum free        500 ml                                 

TEpiCM    扁桃體上皮細(xì)胞培養(yǎng)基         Tonsil Epithelial Cell Medium   500 ml                                 

OKM         口腔角質(zhì)細(xì)胞培養(yǎng)基  Oral Keratinocyte Medium      500 ml                                  【人輪狀病毒抗原(RVAg)ELISA試劑盒現(xiàn)貨】

CoEpiCM  結(jié)腸上皮細(xì)胞培養(yǎng)基     Colonic Epithelial Cell Medium         500 ml                                 

BEpiCM    支氣管上皮細(xì)胞培養(yǎng)基       Bronchial Epithelial Cell Medium     500 ml                                 

SAEpiCM  氣管上皮細(xì)胞培養(yǎng)基  Small Airway Epithelial Cell Medium        500 ml                                 

EpiCM-2   上皮細(xì)胞培養(yǎng)基-2        Epithelial Cell Medium-2 500 ml                                 

UCM         尿道上皮細(xì)胞培養(yǎng)基  Urothelial Cell Medium    500 ml                                 

PEpiCM    前列腺上皮細(xì)胞培養(yǎng)基       Prostate Epithelial Cell Medium       500 ml                                 

STEMium 人胚胎干細(xì)胞無血清培養(yǎng)基         Human Pluripotent Stem Cell Growth Medium       500 ml                                 

STEMium-XF     無異種人胚胎干細(xì)胞生長培養(yǎng)基         Xeno-free Human Pluripotent Stem Cell Growth Medium       500 ml                                 

MEF-cm   小鼠胚胎成纖維細(xì)胞培養(yǎng)基         Mouse Embryonic Fibroblasts-Conditioned Medium        100 ml                                

bFGF-std MEF-cm     堿性成纖維細(xì)胞生長因子刺激的小鼠胚胎成纖維細(xì)胞條件培養(yǎng)基         bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium     100ml                                  

CEpiCM    角膜上皮細(xì)胞培養(yǎng)基  Corneal Epithelial Cell Medium        500 ml                                 

OEpiCM    卵巢上皮細(xì)胞培養(yǎng)基  Ovarian Epithelial Cell Medium        500 ml                                 

MSCM-sf  間充質(zhì)干細(xì)胞培養(yǎng)基  Mesenchymal Stem Cell Medium-serum free 500 ml                                 

MEpiCM   乳腺上皮細(xì)胞培養(yǎng)基  Mammary Epithelial Cell Medium   500 ml                                 

KM-acf     角質(zhì)細(xì)胞培養(yǎng)基  Keratinocyte Medium-animal component free        500 ml                                 

FM-acf      成纖維細(xì)胞培養(yǎng)基       Fibroblast Medium-animal component free    500 ml                                  【人輪狀病毒抗原(RVAg)ELISA試劑盒現(xiàn)貨】

STEMium-ACF  人類多能干細(xì)胞培養(yǎng)基-無動(dòng)物組分   STEMium® Human Pluripotent Stem Cell Growth Medium-animal component free          500ml                                  

MSCM-acf         間充質(zhì)干細(xì)胞培養(yǎng)基     Mesenchymal Stem Cell Medium-animal component free        500 ml                            

MEM        MEM        MEM with Earle＇s Salts ａnd L-Glutamine        500 ml                                 

MEM       MEM       MEM with Hank＇s Salts ａnd L-Glutamine         500 ml                                 

MEM       MEM       MEM with Eagles＇s Salts ａnd Glutamine         500 ml                                 

M199E  M199E  Medium 199 with Earle＇s Salts ａnd L-Glutamine    500 ml                                 

M199H           M199H           Medium 199 with Hanks Salts ａnd L-Glutamine     500 ml                                 

DMEM    DMEM    DMEM with L-Glutamine ａnd Sodium Pyruvate     500 ml                                 

EpiCM       上皮細(xì)胞培養(yǎng)基  Epithelial Cell Medium     500 ml                                 

EpiCM-a   動(dòng)物上皮細(xì)胞培養(yǎng)基  Epithelial Cell Medium-animal          500 ml                                 

MCM        腎系膜細(xì)胞培養(yǎng)基       Mesangial Cell Medium   500 ml                                 

ObM         成骨細(xì)胞培養(yǎng)基  Osteoblast Medium 500 ml                                 

CM   軟骨細(xì)胞培養(yǎng)基     Chondrocyte Medium      500 ml                                 

SM   滑膜細(xì)胞培養(yǎng)基  Synoviocyte Medium        500 ml                                 

NPCM       髓核細(xì)胞培養(yǎng)基  Nucleus Pulposus Cell Medium         500 ml                                 

HM  肝細(xì)胞培養(yǎng)基       Hepatocyte Medium        500 ml                                 

SteCM      星形細(xì)胞培養(yǎng)基  Stellate Cell Medium        500 ml                                 

CMM        心肌細(xì)胞培養(yǎng)基  Cardiac Myocyte Medium        500 ml                                 

TMCM      小梁網(wǎng)細(xì)胞培養(yǎng)基         "Trabecular Meshwork Cell Medium

"       500 ml                                 

TM   滋養(yǎng)層細(xì)胞培養(yǎng)基       Trophoblast Medium        500 ml                                 

AdM          脂肪細(xì)胞培養(yǎng)基     Adipocyte Medium  500ml                                  

PAM 前脂肪細(xì)胞培養(yǎng)基       Preadipocyte Medium     500 ml                                 

PADM       脂肪細(xì)胞誘導(dǎo)分化培養(yǎng)基     Preadipocyte Differentiation Medium     500 ml                                  【人血管緊張素原(aGT)ELISA試劑盒廠家】

MSCM      間充質(zhì)干細(xì)胞培養(yǎng)基  Mesenchymal Stem Cell Medium    500 ml                                 

MODM-500      間充質(zhì)干細(xì)胞-成骨細(xì)胞分化培養(yǎng)基 Mesenchymal Stem Cell Oseogenic Differentiation Medium  500 ml                                 

MODM-250               Mesenchymal Stem Cell Oseogenic Differentiation Medium  250 ml                                 

MADM-500      間充質(zhì)干細(xì)胞-脂肪細(xì)胞分化培養(yǎng)基   Mesenchymal Stem Cell Adipogenic Differentiation Medium 500 ml                                 

MADM-250               Mesenchymal Stem Cell Adipogenic Differentiation Medium 250 ml                                 

MCDM     間充質(zhì)干細(xì)胞-軟骨分化培養(yǎng)基   Mesenchymal Stem Cell Chondrogenic Differentiation Medium     500ml                                  

SMCM-sf  平滑肌細(xì)胞培養(yǎng)基         Smooth Muscle Cell Medium- serum free        500 ml                                 

NPrCM     神經(jīng)前體細(xì)胞培養(yǎng)基     Neural Precursor Cell Medium         500 ml                                 

NM  神經(jīng)細(xì)胞培養(yǎng)基  Neuronal Medium   500 ml