價格詳細資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)產(chǎn)品。

【人5羥色胺(5-HT)ELISA試劑盒現(xiàn)貨】注意事項

1. 當混合蛋白溶液時應盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內(nèi)，如標本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標準曲線，做復孔。

5. 如標本中待測物質(zhì)含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標準品、檢測溶液工作液時，請以相應的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【人5羥色胺(5-HT)ELISA試劑盒現(xiàn)貨】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【人5羥色胺(5-HT)ELISA試劑盒現(xiàn)貨】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【人5羥色胺(5-HT)ELISA試劑盒現(xiàn)貨】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9015186  Tip Guard, M96                 CNY                             

9015094  Vacuum Manifold Top, SBS                CNY                              【人5羥色胺(5-HT)ELISA試劑盒現(xiàn)貨】

9015021  Tubing, retractable tubing, DISU, BR8 RD                CNY                             

1031586  Buffer TCL, 2x (8,5/15),G           CNY                             

1027986  Buffer MRL (90/125),KG            CNY                             

62724       EZ1 DSP Virus Kit (48)      CE-IVD Certified Kit for Extraction of DNA ａnd RNA Viruses from Plasma ａnd Serum. To be used in combination with EZ1 DSP Instrument (SAP 9001360) ａnd EZ1 DSP Virus Card (SAP 9017707). Available Input Volumes 100/200/400 µl, selectable elution volumes 60/90/120/150 µl. Processing time for 6 samples 45 Minutes.     CNY                             

9243521  QIAlink, Maintenance / Support Agreement   Support for QIAlink interface with LIMS system. Includes yearly licensing fees, maintenance cost ａnd software updates. Labor ａnd travel included.     CNY                             

9014405  Connecting plate, BR3000-RapidPlate              CNY                             

9014313  BGR Bottle Holder Rotor                   CNY                             

9018152  Cable, eluate drawer, Qsym              CNY                             

9018151  Eluate drawer, Qsym                 CNY                             

9018141  Heated shaker, Qsym                CNY                             

9018138  PCB, control, et/hs, Qsym                  CNY                             

9018127  Motor, x, extractor, Qsym                 CNY                             

9018088  Cooling adapter, tubes, 2 ml, Qsym                   CNY                             

9018085  Cooling adapter, MTP, RB, Qsym               CNY                             

9018081  Software Package QIAplex MDD              CNY                             

9017853  CD, User Manual QIAcube                 CNY                              【人5羥色胺(5-HT)ELISA試劑盒現(xiàn)貨】

9017699  EX.UNIT, RCS Incubator Tower (65°)                 CNY                             

9015148  Z-Module 300 µl, BR8 RD                  CNY                             

9015072  EX.UNIT, Syringe Pump Plato 3000 (7135)                CNY                             

9015064  Shakerplate, autom., BR8 RD          CNY                             

9019731  Board, A/D, V2, QX           CNY                             

9001550  Rotor-Gene Q 2plex Platform           CNY                             

9013723  Motor-X, RCS1          CNY                             

9013676  Drive Unit MP Holder LH, Shaker BR8000                 CNY                             

9013675  Shakerplate, autom., BR8000          CNY                             

9013435  Maintenance Kit BR 9600 (B)           CNY                             

9013427  Maintenance kit, semi annual, BR9600 (A)               CNY                             

9019655  Quick lock plug, PyroVW           CNY                             

9019621  Tip ejector holder (incl. magnet), Cx2               CNY                             

9019605  Pipettor, spring guard ring (black), Cx2             CNY                             

9019575  Opto switch, X-axis, Cxx            CNY                             

QC088      GenPrep gDNA Plant Kit 植物樣本                                              

QC089      彩虹 預染蛋白質(zhì)分子量標準（窄譜，2-73kDa）    20次/100微升                                             

QC090      彩虹 預染蛋白質(zhì)分子量標準（窄譜，2-73kD）      50次/250微升                                             

QC091      彩虹 預染蛋白質(zhì)分子量標準（窄譜，2-73kDa）     100次/500微升                                           

QC092      彩虹 預染蛋白質(zhì)分子量標準（中譜，2-105kDa）   20次/100微升                                              【人5羥色胺(5-HT)ELISA試劑盒現(xiàn)貨】

QC093      彩虹 預染蛋白質(zhì)分子量標準（中譜，2-105kDa）   50次/250微升                                             

QC094      彩虹 預染蛋白質(zhì)分子量標準（中譜，2-105kDa） 100次/500微升                                           

QC095      彩虹 預染蛋白質(zhì)分子量標準（寬譜，2-220kDa）   20次/100微升                                             

QC096      彩虹 預染蛋白質(zhì)分子量標準（寬譜，2-220kDa）   50次/250微升                                             

QC097      彩虹 預染蛋白質(zhì)分子量標準（寬譜，2-220kDa）   100次/500微升                                           

QC098      窄譜 預染蛋白質(zhì)分子量標準（藍色，2-7lkDa）       20次/100微升                                             

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QC100      窄譜 預染蛋白質(zhì)分子量標準（藍色，2-7lkDa）       100次/500微升                                           

QC101      中譜 預染蛋白質(zhì)分子量標準（藍色，2-105kDa） 20次/100微升                                             

QC102      中譜 預染蛋白質(zhì)分子量標準（藍色，2-105kDa） 50次/250微升                                             

QC103      中譜 預染蛋白質(zhì)分子量標準（藍色，2-105kDa） 100次/500微升                                           

QC105      寬譜 預染蛋白質(zhì)分子量標準（藍色，2-220kDa） 20次/100微升                                             

QC106      寬譜 預染蛋白質(zhì)分子量標準（藍色，2-220kDa） 50次/250微升                                             

QC107      寬譜 預染蛋白質(zhì)分子量標準（藍色，2-220kDa） 100次/500微升                                           

QC108      普通 蛋白質(zhì)分子量標準（低范圍，15-66kDa）      20次/100微升                                             

QC109      普通 蛋白質(zhì)分子量標準（高范圍，15-200kDa）    20次/100微升                                              【人5羥色胺(5-HT)ELISA試劑盒現(xiàn)貨】

QC110      普通 蛋白質(zhì)分子量標準（寬范圍，6.5-200kDa）   20次/100微升                                             

QC111      5x蛋白樣品上樣緩沖液       5 ml                                              

QC112      30%單/雙丙烯酰胺預制膠溶液（19:1）    100ml                                           

QC113      30%單/雙丙烯酰胺預制膠溶液（19:1）    500ml                                          

QC114      30%單/雙丙烯酰胺預制膠溶液（29:1）    100ml                                          

QC115      30%單/雙丙烯酰胺預制膠溶液（29:1）     500ml                                          

QC116      40%單/雙丙烯酰胺預制膠溶液（19:1）    100ml                                          

QC117      40%單/雙丙烯酰胺預制膠溶液（19:1）    500ml                                           

QC118      40%單/雙丙烯酰胺預制膠溶液（29:1）     100ml                                           

QC119      40%單/雙丙烯酰胺預制膠溶液（29:1）    500ml