1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人Ⅰ型膠原吡啶交聯(lián)終肽（ICTP）ELISA試劑盒廠家】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人Ⅰ型膠原吡啶交聯(lián)終肽（ICTP）ELISA試劑盒廠家】齊一生物科技?xì)g迎您訂購(gòu)咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人Ⅰ型膠原吡啶交聯(lián)終肽（ICTP）ELISA試劑盒廠家】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性. 【人Ⅰ型膠原吡啶交聯(lián)終肽（ICTP）ELISA試劑盒廠家】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9002008  Rotor-Gene Q MDx 2plex, Priority  Rotor-Gene Q MDx 2plex, Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.        CNY                             

971460     therascreen® KRAS Pyro Kit (24) CE                   CNY                             

292043     mericon Turkey (24)                   CNY                             

133207     NeXtalStock K/Na tartrate (200)               CNY                             

292073     mericon Ruminant Kit (24)                CNY                              【人Ⅰ型膠原吡啶交聯(lián)終肽（ICTP）ELISA試劑盒廠家】

929104     QIAxcel RNA QC Kit v2.0 (1200)                CNY                             

206042     Type-it Fast SNP Probe PCR Kit (100)        "Features

 Outstanding separation for reliably high call rates

 For difficult SNP loci ａnd for low amounts of template

 Validated using TaqMan SNP Genotyping Assays

 Up to 40% time savings due to fast-cycling procedure

 Convenient ｍaster mix format ａnd optimized protocol"   CNY                             

205314     QuantiTect Reverse Transcription (400)           CNY                             

205313     QuantiTect Rev.Transcription Kit (200)    "For 200 x 20 µl reactions: gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, RNase-Free Water.

The contents of this kit are 4 x the contents of the 205311 (NO bulk!).

Suitable only for cDNA synthesis followed by real-time analyses." CNY                             

291055     mericon GMO Screen CTP2-CP4EPSPS (96)               CNY                             

9001918  PyroMark Q96 MD, PrioPLUS  "PyroMark Q96 MD Instrument

Installation of PyroMark Q96 MD instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

9001914  PyroMark Q24, PrioPLUS "PyroMark Q24 Instrument

Installation of PyroMark Q24 & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10

Loaner available in 2 days*

Annual Inspections (1 onsite or RRC  PM per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)

(In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)"         CNY                             

9001898  QIAxtractor, UV/HEPA (w/o PC) PrioPLUS        "QIAxtractor  Instrument

Installation of QIAxtractor instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

133112     NeXtalStock Sodium formate (200)          CNY                             

965152     QIAamp DNA Blood BioRobot MDx Kit (12)   For 12 x 96 DNA preps: 12 QIAamp 96 Plates,Buffers,† QIAGEN Protease, ElutionMicrotubes CL, Caps, S-Blocks, Tape Pad      CNY                             

962241     QIAprep 96 Plus BioRobot Kit (4)              CNY                             

51204       FlexiGene DNA Kit (50)    For purification of DNA from 50 ml whole blood: Buffers, QIAGEN Protease CNY                             

9015484  Reagent Holder, 6-bottle, 30 ml               CNY                             

9015474  Cable bare, P             CNY                             

9015472  Motor, Y            CNY                             

960141     QIAwell 96 Ultra BioRobot Kit (4)    For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, ａnd QIAprep 96 Plates; Flat-Bottom Blocks ａnd Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads         CNY                              【人Ⅰ型膠原吡啶交聯(lián)終肽（ICTP）ELISA試劑盒廠家】

955134     EZ1 Virus Mini Kit v2.0 (48)               CNY                             

953034     EZ1 DNA Tissue Kit (48)   48 Reagent Cartridges (Tissue), 50 Disposable Tip Holders, 50 Disposable Filter-Tips, 50 Sample Tubes (2.0 ml), 50 Elution Tubes (1.5 ml), Buffer G2, Proteinase K CNY                             

9018454  Pivot pin, N2, w E-clip, QX                 CNY                             

9015457  Motor, Z/P, M96                CNY                             

9015411  Unit, tip connector, M96          CNY                             

9012391  Bottle, system liquid, 10 l, BR8000           CNY                             

133177     NeXtalStock Citric acid pH 2.4 (200)                  CNY                             

291015     mericon Screen 35S Kit (96)              CNY                             

290133     mericon Vibrio triple Kit (24)            CNY                             

290113     mericon Y. enterocolitica Kit (24)              CNY                             

9015384  Unit, spotlight, left, M96                   CNY                             

133081     NeXtalStock PEG 200 (200)               CNY                             

QC203-1   pGEMX -T Easy 載體連接試劑盒  20次                                             

 QY204-1          pBLUE -T 載體連接試劑盒    20次                                              【人Ⅰ型膠原吡啶交聯(lián)終肽（ICTP）ELISA試劑盒廠家】

  QY221-1       通用T載體菌落PCR鑒定試劑盒        80次                                             

 QY222-1          平末端DNA加A試劑盒        25次                                             

 QY223-1          T4 DNA Ligase 50U                                      

 QY301-1          GenFectinTM基因轉(zhuǎn)染試劑  0.5ml                                            

 QY101-01       非預(yù)染低分子量蛋白標(biāo)準(zhǔn)     2050                                              

 QY102-01       非預(yù)染低分子量蛋白標(biāo)準(zhǔn)Ⅰ （66KD,45KD,29KD,15KD.)         2050                                              

QC001      可溶型TMB顯色底物試劑   100ml                                           

QC002      沉淀型TMB顯色底物試劑   500ml                                           

QC003      BCA快速蛋白定量試劑盒     500T                                              

QC004      Bradford快速蛋白定量試劑盒      500T                                              

QC005      蛋白質(zhì)銀染試劑盒         100ml                                           

QC006      SDS-page膠制備試劑盒         50T                                       

QC007      快速考馬斯亮藍(lán)染色液(R-250型）     100ml                                           

QC008      考馬斯亮藍(lán)染色液(常規(guī)法)  250ml                                           

QC009      麗春紅染色液         120ml                                           

QC010      ECL底物發(fā)光試劑盒（試劑A:50ml，試劑B:50ml） 50ml                                               【人Ⅰ型膠原吡啶交聯(lián)終肽（ICTP）ELISA試劑盒廠家】

QC011      HRP-DAB底物顯色試劑盒     60ml                                              

QC012      超敏可逆蛋白顯色試劑         100ml                                           

QC013      堿性磷酸酶底物顯色試劑盒(BCIP/NBT)       100 ml                                          

QC014      胞漿總蛋白提取試劑盒         50T                                       

QC015      植物細(xì)胞質(zhì)膜提取試劑盒     50T                                       

QC016      植物蛋白提取試劑盒     50T                                       

QC017      細(xì)菌總蛋白提取試劑盒         50T                                       

QC018      酵母總蛋白提取試劑盒         50T                                       

QC019      胞漿和核蛋白分步提取試劑盒     50T                                       

QC020      膜蛋白提取試劑盒         50T                                       

QC021      動(dòng)物組織蛋白裂解液     50ml                                              

QC022      真核細(xì)胞蛋白裂解液     50ml                                              

QC023      RIPA裂解液   50ml