1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！【人副流感病毒（HPIV）抗原ELISA試劑盒廠家】

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人副流感病毒（HPIV）抗原ELISA試劑盒廠家】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人副流感病毒（HPIV）抗原ELISA試劑盒廠家】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性.

以上資料僅供參考了解，不供于論文寫作

【人副流感病毒（HPIV）抗原ELISA試劑盒廠家】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費(fèi)代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

9230281 Cable, Interface, Plato-Syringe Pump        CNY            

9235253 Trough-Rack spezial     CNY            

9230197 Plug f. dilutor valve inlet     CNY             【人副流感病毒（HPIV）抗原ELISA試劑盒廠家】

9230059 Rack for reagents, Trough(for 3 troughs)        CNY            

9238674 Bracket for grey wedge      CNY            

9238652 Interference suppression capacitor      CNY            

9238636 Gear motor, lid opening     CNY            

9242027 Repair fee, Depot Repair, Palm Cycler       CNY            

9241822 Pyro Q24, Full Agreement    """Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

 or Repair Center Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage""" CNY            

9241819 Pyro Q96 ID, Basic Agreement    "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9240975 PCB, control, sample tracking, Qsym     CNY            

9240969 PCB, carriersens, sample tracking, Qsym     CNY            

9240682 Dump Tray, Overflow Control, AP98       CNY            

9240789 Update,Unload Valve Assy, AP196/98      CNY            

9240815 #Basic Cover Agreement EZ1 DSP, 1YR     CNY            

9240775 Sample Rack, AP     CNY            

6000-5017   MST Conv.Rack&Lid (Silver - 2 pos. cal.)        CNY            

9241762 QIAgility, Basic Agreement  "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) or PC repair provision: not included

Transportation cost coverage: not applicable"   CNY            

9241380 24VDC Adaptor Cable, Microlynx, AP      CNY             【人副流感病毒（HPIV）抗原ELISA試劑盒廠家】

9240758 Update, Bracket, Shaker Bimba, AP98     CNY            

9243552 Target Prep, PM Service "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY            

9243384 Cooling adapter, SBS Universal, Qsym        CNY            

9243116 Lee Sample Valve Kit, LC        CNY            

9243234 HC2, PM Agreement   The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP CNY            

9243044 Set, brackets, transparent panel, Qsym      CNY            

9242752 Kit, upg-x/y-drive, portal, Qsym        CNY            

9242711 Validation Support, 2d, QIAsymphony The QIAGEN QIAsymphony Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Applications Specialist.  A QIAGEN specialist will provide an introduction to the QIAsymphony system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on QIAsymphony instrument operation.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates. The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to include in the validation (Casework200, Casework500, Reference200, etc.).    CNY            

9242203 Cooling adapter, SBS Universal, Qsym        CNY            

9242198 Lid Sealing Ring, Eppendorf, AP196/98       CNY            

5000-1402   Latch Front Assembly, VTX2      CNY            

5000-1315   Rotary Shaker - Spring Single       CNY            

5000-1308   Sensor Speed, SKR1      CNY            

5000-1280   Bottom Feet W Screws, DML2      CNY            

QTR-919 癌胚抗原(CEA)檢測卡 1T  20T、50T/盒            

QTR-920 前列腺特異性抗原(PSA)檢測卡 1T  20T、50T/盒            

QTR-921 布病阻斷ELISA抗體檢測試劑盒                       

QTR-922 賈弟蟲抗原檢測試紙條                        【人副流感病毒（HPIV）抗原ELISA試劑盒廠家】

QTR-923 A型輪狀病毒ELISA抗原檢測試劑盒                    

QTR-924 A型輪狀病毒抗原檢測試紙條                      

QTR-925 西尼羅河熱阻斷ELISA抗體檢測試劑盒                     

QTR-926 小球隱孢子蟲檢測試劑條                     

QTR-927 A型流感阻斷ELISA抗體檢測試劑盒                    

QTR-928 豬胸膜肺炎放線桿菌iELISA抗體檢測試劑盒（10,12型）                     

QTR-929 豬胸膜肺炎放線桿菌iELISA抗體檢測試劑盒（1,2,9,11/3,6,8,15/4,5,7型）                       

QTR-930 豬偽狂犬病病毒(全)抗體檢測試劑盒                       

QTR-931 豬偽狂犬gB-ELISA抗體檢測試劑盒                    

QTR-932 豬偽狂犬gE-ELISA抗體檢測試劑盒                    

QTR-933 非洲豬瘟抗原檢測ELISA試劑盒                       

QTR-934 非洲豬瘟cELISA抗體檢測試劑盒                      

QTR-935 非洲豬瘟病毒抗體檢測試紙條                     

QTR-936 豬布病iELISA抗體檢測試劑盒                    

QTR-937 副豬嗜血桿菌iELISA抗體檢測試劑盒                      

QTR-938 流感iELISA抗體檢測試劑盒                       【人副流感病毒（HPIV）抗原ELISA試劑盒廠家】

QTR-939 雙抗夾心法豬圓環(huán)病毒抗原檢測試劑盒                     

QTR-940 PCV-2 IgG/IgM抗體檢測試劑盒                    

QTR-941 PCV-2 IgG抗體檢測試劑盒                    

QTR-942 豬丹毒iELISA抗體檢測試劑盒                    

QTR-943 豬肺炎支原體ELISA抗體檢測試劑盒                       

QTR-944 豬細(xì)小病毒iELISA抗體檢測試劑盒                    

QTR-945 豬細(xì)小病毒ELISA抗原檢測試劑盒                     

QTR-946 豬傳染性胃腸炎/豬冠狀病毒檢測試劑盒（Porcine Coronavirus(PRCV/TGEV)）                      

QTR-947 豬傳染性胃腸炎和冠狀病毒二聯(lián)抗體檢測試劑盒                     

QTR-948 豬冠狀病毒ELISA抗體檢測試劑盒                     

QTR-949 豬傳染性胃腸炎ELISA抗體檢測試劑盒