1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！【人抗核周因子抗體(APF)ELISA試劑盒廠家】

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強,重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【人抗核周因子抗體(APF)ELISA試劑盒廠家】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【人抗核周因子抗體(APF)ELISA試劑盒廠家】ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性.

以上資料僅供參考了解，不供于論文寫作

【人抗核周因子抗體(APF)ELISA試劑盒廠家】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細胞菌株、培養(yǎng)基、細胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

133115  NeXtalStock Sodium sulfate (200)        CNY            

965662  QIAamp Virus BioRobot 9604 Kit (12) For 12 x 96 nucleic acid preps: 12 QIAamp 96 Plates, RNase-free Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Carrier RNA, Caps   CNY            

9240670 Reservoir, 1000ml, AP96/196     CNY            

9240644 External BCR, Metrologic MS9521, AP     CNY            

9240636 Power Entry Module, AP196/98        CNY            

9240616 Fuse, 5.0A, FNM, AP96       CNY            

9240607 Fiber Optic Sensor Cable, 5M, AP98      CNY            

9240590 Purge Solenoid, Overflow Jar, AP96/196      CNY            

9240573 Centrifuge RT-7, Sorvall, 115V, AP96        CNY            

9240551 Main PCB, Eppendorf, AP196/98       CNY            

9240533 Motor Assy, SiNug, 23, CapU/D, AP96     CNY            

9240524 Cable, Motor, Axis#1, Capper U\D, AP98      CNY            

9240347 Autopure, Basic Agreement   "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9240243 Brushes, with spring (2), TR        CNY            

9240097 Re-Installation, BR The Re-Installation, BR facilitates quick implementation of the unit back into lab routine by minimizing  instrument set-up phase. It covers hardware ａnd software check of  instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.    CNY             【人抗核周因子抗體(APF)ELISA試劑盒廠家】

9239568 Needle, W-Drive, DISU, BR8, v2.0        CNY            

9239561 Assy,Kit,Retrofit,Coherent-intoLX100        CNY            

9019936 Pipetting head, 8 ch. , CAS3800/4800        CNY            

9019491 PLATE 384 WELL ADAPTOR GENERIC      CNY            

9238885 Cable, magnet lift, BS96        CNY            

9238883 Keypad, BS96        CNY            

9238865 #BR M96, Premium Agreement  "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9238859 #Full Agreement MDx, 2PM, 1yr   The Full Agreement MDx, 2 PM provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days). CNY            

9241219 Warranty Performance Package, RCS       CNY            

9241209 QIAsymphony SP, PM Agreement    An agreement for preventive maintenance provides coverage for travel, labor, ａnd parts, plus the cost of consumables required for the maintenance visit. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives. CNY            

9241212 Full Agreement Depot Repair, MSTVtx, 1yr    "The Full Agreement Depot Repair, MSTVtx provides full service coverage for the Multi-Specimen Tube Vortexer used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."   CNY            

9241185 Sensor, interlock1, with cable, QX      CNY            

9238823 Rect. carrier for 1 bottle 200 ml w/ cap        CNY            

9238816 Rect. carrier for 12 Vacutainertubes 7ml        CNY            

9233298 Toothbelt, X-drive, P3xx0       CNY            

9237712 #BR M96, Installation       CNY             【人抗核周因子抗體(APF)ELISA試劑盒廠家】

9233267 Motor, Y-drive, BR8000      CNY            

9233211 Tubing, PVC transp., D5/d3mm, (per m)       CNY            

NG0266  脂蛋白a[LPa]測試盒  96孔    ELISA法            

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NG0271  羥脯氨酸測試前處理試劑  50管/48樣 比色法             

NG0272  羥脯氨酸Hyp測試盒  50管/48樣 比色法             

NG0273  谷氨酰胺合成酶GS測試盒 50管/48樣 比色法              【人抗核周因子抗體(APF)ELISA試劑盒廠家】

NG0274  單胺氧化酶MAO測試盒    50管/48樣 紫外比色法             

NG0275  5’－核苷酸酶5’－NT測試盒 50管/24樣 比色法             

NG0276  腺苷脫氨酶ADA測試盒    50管/24樣 比色法             

NG0277  前列腺酸性磷酸酶PACP測試盒 50管/25樣 比色法             

NG0278  抗酒石酸酸性磷酸酶StrACP測試盒 50管/24樣 比色法             

NG0279  堿性磷酸酶AKP測試盒    50管/48樣 比色法             

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NG0284  總蛋白定量測試盒帶標(biāo)準(zhǔn)；考馬斯亮蘭法    100管/96樣 比色法             

NG0285  總蛋白定量測試盒帶標(biāo)準(zhǔn)；考馬斯亮蘭法    50管/48樣 比色法             

NG0286  蛋白標(biāo)準(zhǔn)品總蛋白、白蛋白                       

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NG0289  總鐵結(jié)合力TIBC測試盒   50管/48樣 比色法              【人抗核周因子抗體(APF)ELISA試劑盒廠家】

NG0290  髓過氧化物酶MPO測試盒  50管/24樣 比色法             

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