齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測(cè)測(cè)定的精度和提高檢測(cè)結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個(gè)試劑盒采用stripwell微孔板。這允許用戶在不同的時(shí)間來(lái)分析不同數(shù)量的樣品。

3。什么類(lèi)型的可重復(fù)性的結(jié)果，得到與分析？

每一個(gè)工具都有一個(gè)包含一個(gè)典型數(shù)據(jù)的圖表的手冊(cè)。在運(yùn)營(yíng)商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時(shí)間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個(gè)用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲(chǔ)存的試劑比表示？

在不推薦使用條件下的部件組件的存儲(chǔ)，以保證測(cè)試的正確性能。

5。我應(yīng)該如何儲(chǔ)存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長(zhǎng)期儲(chǔ)存，建議凍結(jié)他們?cè)?/span>- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會(huì)給出錯(cuò)誤的結(jié)果。

7。我可以使用一個(gè)不建議在試劑盒插入的樣本類(lèi)型嗎？

該試劑盒已被驗(yàn)證在試劑盒插入的樣品類(lèi)型。未經(jīng)檢驗(yàn)的樣品類(lèi)型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動(dòng)態(tài)范圍內(nèi)的測(cè)定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測(cè)定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測(cè)的。

9。我每次都要運(yùn)行一個(gè)空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計(jì)算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測(cè)到檢測(cè)。當(dāng)排除特定的檢測(cè)問(wèn)題的來(lái)源時(shí)，它們也非常有幫助。

10。我能改變我在檢測(cè)中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計(jì)為*佳的性能在給定的體積

11。可以使用不同的組件組件嗎？

每一個(gè)組件包含有特定批號(hào)的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對(duì)這些特定的地段進(jìn)行質(zhì)量檢驗(yàn)。這是從來(lái)沒(méi)有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來(lái)很好，但我沒(méi)有得到一個(gè)信號(hào)，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個(gè)矩陣的效果可能是掩蓋檢測(cè)。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋?zhuān)?qǐng)檢查是否正確進(jìn)行稀釋。過(guò)稀釋會(huì)導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤(pán)子？

如果您使用的是一個(gè)自動(dòng)平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動(dòng)洗衣機(jī)也是一樣的。一個(gè)中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無(wú)塵紙。【人磷酸肌醇3激酶(PI3K)ELISA試劑盒廠家】

14。我需要用一個(gè)盤(pán)子嗎？

可靠的結(jié)果可以得到無(wú)板振動(dòng)，但外徑的一般會(huì)低于那些使用平板振動(dòng)器。

15。為什么我要用波長(zhǎng)校正450-570nm之間？

對(duì)于酶聯(lián)免疫吸附試驗(yàn)，在雙波長(zhǎng)讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動(dòng)。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過(guò)程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個(gè)給定的分析物的量可能會(huì)有所不同，不僅從物種的物種，但也組織和細(xì)胞來(lái)源。這個(gè)信息的*佳來(lái)源是目前的文獻(xiàn)，很容易通過(guò)互聯(lián)網(wǎng)在多個(gè)科學(xué)數(shù)據(jù)庫(kù)訪問(wèn)。

18。我的光密度有點(diǎn)高（或較低），比那些在手冊(cè)中，與我的試劑盒來(lái)了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【人磷酸肌醇3激酶(PI3K)ELISA試劑盒廠家】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【人磷酸肌醇3激酶(PI3K)ELISA試劑盒廠家】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【人磷酸肌醇3激酶(PI3K)ELISA試劑盒廠家】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9243110  CD-RW, Black, AP196/98           CNY                             

9242712  Validation Support, 2d QIAcube       The QIAGEN QIAcube Guided Validation includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on QIAcube operation.  The Guided Validation service includes experimental design consulation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.)      CNY                             

9019226  Fan - rear 92x92, PyroQ24                 CNY                             

9019220  PCB - Distribution robot, PyroQ96MD               CNY                             

1066843  PG Influenza A RT-PCR Assay (48) LE Z              CNY                             

1066848  NDV Univ. RT-PCR REAGENT LE                 CNY                             

1066854  AIV-H9 RT-PCR REAGENT LE             CNY                             

1060992  Buffer MW1 (77ml)           CNY                             

1061059  Sample Preparation Reagents, 8ml                   CNY                             

1061080  NG Quantification Standards            CNY                             

1061113  PG AIV-H7 RT-PCR Assay (48) Z                  CNY                             

9016568  Plate Grippers, RCS1                  CNY                              【人磷酸肌醇3激酶(PI3K)ELISA試劑盒廠家】

9016536  Transportation box , BioSprint 15             CNY                             

9016535  Transportation box , Twister II                   CNY                             

9016533  Transportation box , RapidPlate onlf.                CNY                             

9016530  Transportation box 1, LiquiChip                 CNY                             

9016493  BR MDx DSP Service, PRO                 CNY                             

9016325  Washer head with holder , P 13xx            CNY                             

9019185  PCB - Front, PyroQ96MD          CNY                             

9011902  Upgrade, tip change system             CNY                             

9011824  Z-Modul; Tip-Adapter 1100µl; BR8000            CNY                             

9237033  Cable, Liquid Sensor, BR8 RD v1.0            CNY                             

9236960  Probe Holder, Body, BR8 RD             CNY                             

9019144  Fan - rear 92x92, PyroQ96MD                   CNY                             

9019113  PCB - Camera, PyroQ96ID                 CNY                             

9019075  PyroMark NDT Holder, PyroQ96MD        Re-usable holder for tips, Nucleotide Dispensing Tips ａnd Reagent Dispensing Tips, for use in PyroMark Q96 MD.  CNY                             

9019010  Motor M, EZ1xl                 CNY                             

9018953  Reagent Block, 16x0.2ml/2ml PCR/Flat-Bas              CNY                              【人磷酸肌醇3激酶(PI3K)ELISA試劑盒廠家】

9018938  Adapter, Light Cycler 480 384-Well Plate                  CNY                             

9018935  Adapter, reagent tube SBS Plate              CNY                             

9015837  Upg. Kit, Y6L Connection, BR8 (ISI21)               CNY                             

9015689  Fittings (10), M6/3.3 mm, white                CNY                             

9015605  Upgrade, LiquiChip IS 2.x, PLUS                 CNY                             

QY-x2279 H-4-II-E 大鼠肝細(xì)胞瘤細(xì)胞                                                

QY-x2280 H9 人急性淋巴母細(xì)胞白血病細(xì)胞                                                    

QY-x2281 HAC-84 人肺泡癌細(xì)胞                                                

QY-x2282 Hacat 人永生化角質(zhì)細(xì)胞                                                   

QY-x2283 HAL-01 人急性混合型白血病細(xì)胞                                                     

QY-x2284 HAN 人羊膜細(xì)胞                                                 

QY-x2285 H-bc 人膀胱癌細(xì)胞                                                     

QY-x2286 HBF 人膀胱組織來(lái)源細(xì)胞                                                  

QY-x2287 NRK-52E 大鼠腎細(xì)胞DMEM培養(yǎng)基，添加4 mM L-谷氨酰胺，含1.5 g/L 碳酸氫鈉4.5 g/L 葡萄糖, 95%; 小牛血清, 5%                                                        

QY-x2288 NRK大鼠腎細(xì)胞含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 4mM L-谷氨酰胺的DMEM，90%；胎牛血清或新生牛血清，10%。 ： -                                                  

QY-x2289 NIH/3T3 鼠成纖維細(xì)胞系 含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖，4mM L-谷氨酰胺的DMEM，90%；BCS，10%。 ： 與原始的...                                                  

QY-x2290 NCI-N87 [N87]人胃癌細(xì)胞 RPMI 1640 medium with 2 mM L-glutamine adjusted to contain ...                                                       【人磷酸肌醇3激酶(PI3K)ELISA試劑盒廠家】

QY-x2291 NCI-H292人肺癌細(xì)胞(淋巴結(jié)轉(zhuǎn)移)含2mM L-谷氨酰胺，10mM HEPES，1mM 丙酮酸鈉，4.5g/L 葡萄糖，1.5g/L ...                                               

QY-x2292 NAMALWA 人Burkitt's淋巴瘤細(xì)胞B 淋巴細(xì)胞；Burkitt淋巴瘤形態(tài)： 淋巴母細(xì)胞含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 10mM HEPE...                                                    

QY-x2293 MRC-5 人胚肺細(xì)胞 含1.0mM丙酮酸鈉，0.1mM非必需氨基酸和1.5克/升碳酸氫鈉的EMEM，90%；胎牛血清，10%。 ： MRC-5細(xì)胞系來(lái)自...                                                        

QY-x2294 MGC80-3 人胃癌細(xì)胞RPMI 1640+10%胎牛血清或新生牛血清 ： 從一位53歲 性原發(fā)性胃低分化粘液腺癌患者建立。                                                       

QY-x2295 MFC小鼠胃癌細(xì)胞 RPMI 1640+10%胎牛血清或新生牛血清 ： 源自615小鼠。狗腎細(xì)胞 MDCK (NBL-2) 詢價(jià) ATCC                                                        

QY-x2296 MDCK (NBL-2) 狗腎細(xì)胞含 1.5g/L 碳酸氫鈉，0.1mM 非必需氨基酸，1.0mM 丙酮酸鈉，2mM L-谷氨酰胺和Earle'...                                                        

QY-x2297 MDCK犬腎細(xì)胞系添加2mM L-谷氨酰胺，1.0mM丙酮酸鈉，0.1mM非必需氨基酸，1.5g/L碳酸氫鈉的EMEM，90%；FBS，10%。 ： 1...                                                   

QY-x2298 MDBK (NBL-1) 牛腎細(xì)胞 含 1.5g/L 碳酸氫鈉，0.1mM 非必需氨基酸，1.0mM 丙酮酸鈉，2mM L-谷氨酰胺和Earle&#39...                                                  

QY-x2299 MDA-MB-453 人乳腺癌細(xì)胞 L-15+10%胎牛血清 ： 從胸水中建立的人乳腺癌細(xì)胞株。                                                

QY-x2300 MDA-MB-231 人乳腺癌細(xì)胞轉(zhuǎn)移性胸膜滲出液形態(tài)：Leibovitz’s L15培養(yǎng)液，添加10%胎牛血清。 ： MDA-MB-231來(lái)...                                                       

QY-x2301 MCF7人乳腺癌細(xì)胞EMEM加1.0mM丙酮酸鈉，0.1mM非必需氨基酸和1.5克/升碳酸氫鈉，添加0.01毫克/毫升牛胰島素和10%胎牛血... 恒河猴腎細(xì)胞 LLC-MK2 詢價(jià) ATCC                                                

QY-x2302 LLC-MK2 恒河猴腎細(xì)胞 含1.68g/L碳酸氫鈉的199培養(yǎng)基，99%；新生牛血清，1%。 ： LLC-MK2細(xì)胞株源自六只成年綠猴的腎細(xì)胞懸液。 據(jù)報(bào)...                                                     

QY-x2303 LLC小鼠肺癌細(xì)胞 DMEM+10%胎牛血清或新生牛血清 ： 小鼠Lewis肺癌細(xì)胞                                               

QY-x2304 L929小鼠成纖維細(xì)胞RPMI 1640+10%胎牛血清或新生牛血清 ： 親本L的亞克隆，是*早建立的連續(xù)培養(yǎng)物。 L細(xì)胞源自100天的...                                                        

QY-x2305 L6 大鼠成肌細(xì)胞含4mM L-谷氨酰胺，1.0mM 丙酮酸，4.5g/L 葡萄糖和1.5g/L碳酸氫鈉的DMEM 90%；胎牛血清 10%。 ...                                                     

QY-x2306 Jurkat人T淋巴細(xì)胞白血病細(xì)胞 RPMI-1640 10%FBS ： 細(xì)胞株來(lái)源于一個(gè)14歲 孩的外周血。 經(jīng)佛波酯和外源凝集素或抗T3單克...                                                       【人磷酸肌醇3激酶(PI3K)ELISA試劑盒廠家】

QY-x2307 Huh-7 人肝癌細(xì)胞 DMEM 10%FBS ： 據(jù)說(shuō)產(chǎn)甲胎蛋白，胰酶α抗體，血漿銅藍(lán)蛋白，纖維蛋白原，纖維粘連蛋白等。                                                      

QY-x2308 HT-29 人結(jié)腸癌細(xì)胞 McCoy’s 5a 10%FBS ： 1964年，該細(xì)胞系由J.Fogh 用移植培養(yǎng)方法和含15%FBS的F12培...                                                      

QY-x2309 HT-1080 人纖維肉瘤細(xì)胞系DMEM 10%FBS ： 這株細(xì)胞含有活化的N-ras癌基因。                                              

QY-x2022    CEM 白血病 1640+10%胎牛血清                    

QY-x2023    K562 慢性髓原白血病 1640+10%胎牛血清                       

QY-x2024    HPB-ALL T細(xì)胞白血病 1640+10%胎牛血清                       

QY-x2025    HL-60 白血病 1640+10%胎牛血清                      

QY-x2026    Daudi B淋巴細(xì)胞瘤 1640+10%胎牛血清                     

QY-x2027    SK-BR-3 乳腺腺癌 1640+15%胎牛血清                      

QY-x2028    MCF-7 乳腺腺癌 1640+10%胎牛血清                    

QY-x2029    MCF-7/Adr 乳腺腺癌/阿霉素耐藥株 1640+10%胎牛血清                       

QY-x2030    A2780 卵巢癌 1640+10%胎牛血清