規(guī)格:小規(guī)格48T.大規(guī)格96T

樣本標(biāo)準(zhǔn)：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：酶聯(lián)免疫分析（ELISA）

運(yùn)輸保存：2~8°貯存.保質(zhì)期：6個(gè)月

試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人磷酸果糖激酶(PFK)ELISA試劑盒現(xiàn)貨】齊一生物主要經(jīng)營進(jìn)口原裝Elisa試劑盒,檢測試劑種類齊全,價(jià)格實(shí)惠！購買齊一生物產(chǎn)品的新老客戶都可享免費(fèi)代測實(shí)驗(yàn)服務(wù),全程提供技術(shù)支持.如需其它Elisa試劑盒說明書及產(chǎn)品價(jià)格,可來電咨詢齊一生物銷售：021-6034 8496；181214 53965；173021 04490

試劑盒特點(diǎn)

一、高效、靈敏、特異的抗體；

二、穩(wěn)定的重復(fù)性和可靠性；

三、吸附性能好,空白值低,孔底透明度高的固相載體；

四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型；

試劑盒可概括四個(gè)方面：

1、免疫酶染色各種細(xì)胞內(nèi)成份的定位.

2、研究抗酶抗體的合成.

3、顯現(xiàn)微量的免疫沉淀反應(yīng).

4、定量檢測體液中抗原或抗體成份.

【人磷酸果糖激酶(PFK)ELISA試劑盒現(xiàn)貨】ELISA試劑盒為您節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).

elisa試劑盒回收率是反應(yīng)待測物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說明方法的準(zhǔn)確度.比如水中總無機(jī)氯含量測定,樣品水中含有無機(jī)氯20mg/L,取100mL被測水樣品,加入0.1mL濃度為10mg/mL的含無機(jī)氯標(biāo)準(zhǔn)樣品,測定時(shí)忽略體積變化,如果測定出樣品中無機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.

【人磷酸果糖激酶(PFK)ELISA試劑盒現(xiàn)貨】試劑盒制備方法包括以下步驟：

1)抗原表位的計(jì)算機(jī)篩選；

2)抗原的制備；

3)血清的采集；

4)間接ELISA方法的建立；

5)間接ELISA方法的敏感性和特異性驗(yàn)證；

6)試劑盒的穩(wěn)定性驗(yàn)證；

7)對(duì)大量樣本進(jìn)行檢測.該方法簡單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出樣本含量.適用于大批量樣本的檢測

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

【人磷酸果糖激酶(PFK)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！

9020586  Power Supply, 24V 60W, DML 3000           CNY                           

9013314  Shaker adapter, 192-tube, 5 ml             CNY                           

9013211  Cabling kit, data, BR8000             CNY                            【人磷酸果糖激酶(PFK)ELISA試劑盒現(xiàn)貨】

9011747  Worktable left, BR8000 v1.01              CNY                           

132568    NeXtal Stock Methoxy-PEG 5000 (50) 30% w/v Methoxypolyethylene glycol 5000 - 50mL (4 x 12,5 ml tube)      CNY                           

800311    Custom Primer              CNY                           

766134    PAXgene Tissue miRNA Kit (50)          CNY                           

203443    HotStarTaq Master Mix Kit (250)  "3 x 0.85 ml HotStarTaq Master Mix* containing 250 units HotStarTaq DNA Polymerase total, 2 x 1.7 ml distilled water

- premixed solution for high PCR specificity

- HotStarTaq Master Mix, HotStarTaq DNA Polymerase, several kit sizes from 250 u up to 2500 u available" CNY                           

201901    dNTP Mix, PCR Grade, 10mM, 4x200µl      "dNTP Mix contains dATP, dCTP, dGTP, ａnd dTTP in water (pH 7.5), 10mM each.

for 800 reactions based on 50ul PCR (0.2uM each dNTP)" CNY                           

201209    Taq DNA Polymerase (25000)      "100 x 250 units Taq DNA Polymerase, 100 x 1.2ml PCR Buffer, 100 x 2.0ml Q-Solution, 100 x 1.2ml MgCl2

NEW! Now with 100 x 1,2 ml CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."  CNY                           

200203    TopTaq DNA Polymerase (250)   "1 x 250U TopTaq DNA Polymerase, 10x TopTaq PCR Buffer, 1x CoralLoad Concentrate, 5x Q-solutions, 25mM MgCl2

For highly reliable end-point PCR with unrivalled ease-of-use

The first fridge storage enzyme"   CNY                           

180462    GeneRead DNA Library L Core Kit (12)              CNY                           

159916    Generation Capture Column Kit (300)          CNY                           

159104    QIAsafe DNA Tubes (50)             CNY                           

158902    RBC Lysis Solution(450 ml)          CNY                           

678791    ipsogen MLL-AF9 type B e8e9 Standards            CNY                           

678391    ipsogen MLL-AF4 e9e5 Standards        CNY                           

34860     Anti-GST Antibody (100µg)         CNY                           

9021057  Door Assy, Lower Right, DCU             CNY                            【人磷酸果糖激酶(PFK)ELISA試劑盒現(xiàn)貨】

9021056  Door Assy, Lower Left, DCU              CNY                           

9021002  Belt, Y Drive, DCU        CNY                           

9020994  De-Capper Assembly, DCU           CNY                           

9020931  Fixation angles, vac.pump v2.0, BR8000             CNY                           

9020906  HEPA filter system, SMC, QGX           CNY                           

9020704  Lid frame with hinge C1x             CNY                           

9020638  Baseboard, side, Cabinet Qsym            CNY                           

9020634  Panel, left, Cabinet Qsym             CNY                           

9020626  Holder, BC scanner, Cabinet Qsym              CNY                           

9020333  Fuse - Thermal (5), RGQII          CNY                           

9017687  AUTOPURE Pro K Pump             CNY                           

9017602  Pump, MultiFlow Disp, BR8 RD          CNY                           

9017503  Bearing, teflon, white (5), TR              CNY                           

9017257  BR 8000, Protein Purification Package         CNY                           

QY-x2062       MPC-83 胰腺腺泡細(xì)胞癌 1640+10%胎牛血清 體外培養(yǎng)株                                    

QY-x2063       MPC-83 胰腺腺泡細(xì)胞癌 瘤株                                    

QY-x2064       RIN-m5F β胰島素瘤 1640+10%胎牛血清 體外培養(yǎng)株                                   

QY-x2065       MA891 乳腺癌 瘤株                                      

QY-x2066       TA2 自發(fā)高乳腺癌 瘤株                                        【人磷酸果糖激酶(PFK)ELISA試劑盒現(xiàn)貨】

QY-x2067       U14 宮頸癌 瘤株                                   

QY-x2068       YAC-1 淋巴瘤 1640+10%胎牛血清 體外培養(yǎng)株                                       

QY-x2069       Nb2－11 淋巴瘤 體外培養(yǎng)株                                       

QY-x2070       C26 結(jié)腸癌 瘤株                                   

QY-x2071       G422 神經(jīng)膠質(zhì)瘤 瘤株                                        

QY-x2072       B16 黑色素瘤 1640+10%胎牛血清 體外培養(yǎng)株                                       

QY-x2073       B16 黑色素瘤 瘤株                                       

QY-x2074       C3 白血病 體外培養(yǎng)株                                         

QY-x2075       L1210 淋巴白血病 1640+10%胎牛血清 體外培養(yǎng)株                                       

QY-x2076       L1210 淋巴白血病 瘤株                                       

QY-x2077       SP2/0 骨髓瘤 1640+10%胎牛血清 體外培養(yǎng)株                                        

QY-x2078       NS-1 骨髓瘤 1640+10%胎牛血清 體外培養(yǎng)株                                         

QY-x2079       P388D 淋巴母細(xì)胞瘤 1640+10%胎牛血清 體外培養(yǎng)株                                   

QY-x2080       Kert-3 腎癌 瘤株                                    

QY-x2081       TC-1 HPVl6 E6、E7和ras基因共轉(zhuǎn)化的C57BL／C(H-2b)小鼠肺上皮細(xì)胞 1640+10%胎牛血清體外培養(yǎng)株                                    

QY-x2082       W256 肉瘤(Wistar) 瘤株                                        【人磷酸果糖激酶(PFK)ELISA試劑盒現(xiàn)貨】

QY-x2083       PC-12 嗜鉻細(xì)胞瘤 1640+10%胎牛血清+5%馬血清 體外培養(yǎng)株                                    

QY-x2084       [編輯本段]細(xì)胞庫部分細(xì)胞系目錄（遺傳和正常細(xì)胞）                                   

QY-x2085       細(xì)胞名稱 組織類型 培養(yǎng)條件                                     

QY-x2086       HIH 3T3 小鼠胚胎成纖維細(xì)胞 1640+10%胎牛血清                                         

QY-x2087       L929 小鼠成纖維細(xì)胞 1640+10%胎牛血清                                       

QY-x2088       HSC-T6 大鼠肝星狀細(xì)胞 1640+10%胎牛血清                                   

QY-x2089       L-02 人肝細(xì)胞 1640+10%胎牛血清                                    

QY-x2090       SRA01/04 人晶體上皮細(xì)胞 MEM+非必需氨基酸＋10%胎牛血清                                   

QY-x2091       CHO 中國倉鼠卵巢細(xì)胞（野生型） 1640+10%胎牛血清                                        

QY-x2092       IEC-6 大鼠小腸隱窩上皮細(xì)胞 DMEM+10%胎牛血清