1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！【人血清白蛋白(HSA)ELISA試劑盒廠家】

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類(lèi)戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【人血清白蛋白(HSA)ELISA試劑盒廠家】齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【人血清白蛋白(HSA)ELISA試劑盒廠家】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性.

以上資料僅供參考了解，不供于論文寫(xiě)作

【人血清白蛋白(HSA)ELISA試劑盒廠家】齊一生物專(zhuān)業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

9241769 QIAxtractor, Full Agreement The Full Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included . With a Full Agreement, there is a priority response time of 48 hours (2 working days).  CNY            

9241368 Interlock, IL1, EZ1a        CNY            

9241366 Cable, UV lamp voltage converter, EZ1a      CNY            

9240762 Co-Processor, AP96      CNY             【人血清白蛋白(HSA)ELISA試劑盒廠家】

9240760 Update, Bucket/Elevator Head, AP196/98      CNY            

9243549 Pyro Q96 MD, PM Service "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY            

9242786 RCS, Full Agreement, 2PM    "Repair service delivery: onsite ａnd to Regional Repair Center

Service response time (repair): 48 hours/2 business days or 7-10 working days for RRC repairs

Periodic inspection/maintenance: yes, 2/agreement year

Inspection/maintenance delivery: onsite for RCS only

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, included"    CNY            

9242714 Validation Support, 5d, QIAgility   The QIAGEN QIAgility Accelerated Validation Support service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the QIAgility system ａnd software, review the User Manual, as well as hands-on user support ａnd QIAgility instrument operation.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates. The QIAGEN Specialist will support the customer by running samples through the QIAGEN-only extraction method/system (does not include phenol-chloroform or similar methods), as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study. The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product. Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased to cover the validation experimental design ａnd are not included in the cost of validation support.  Please indicate which processes (quant setup, normalization, PCR setup, CE setup) ａnd kit manufacturer (Applied Biosystems, Promega, QIAGEN) need to be included in the validation. CNY            

9242531 QIAsymphony SPAS, PM Agreement      CNY            

9242546 Probe Holder Assembly, LX200, LC        CNY            

9242152 Upgrade, CAS1820 vacuum DX/VX   "The Upgrade, CAS 1820 vacuum DX/VX upgrades the X-tractorgene to use the improved vacuum system for the QIAxtractor. The upgrade is conducted as an on-site service, performed by a QIAGEN service specialist.

The upgrade allows for the usage of the QIAGEN DX ａnd VX capture plates ａnd the disposable silicon mats.

During the service, the new vacuum system is installed ａnd the software is updated. Please note that due to the software upgrade the instrument will only run the QIAxtractor software applications. Customized run files from the previous version will have to be adapted beforehand for additional fees."  CNY            

5000-1404   Plate Cartridge 110V, VTX2      CNY            

5000-1277   Top Housing W LID, DML2     CNY            

5050-1029   Mouse, PC System spare      CNY            

5025-1011   Expand 4 Pipettor 240V      CNY            

9240747 Belt, Reagent Pump 117 groove, AP       CNY            

9240720 Cable Carrier, Tubing, Dispense Head, AP        CNY            

9240700 Tubing, Waste, 1 1\4", AP       CNY            

9243690 DML Luminometer, Repair Fee DML Repair Fee covers Regional Repair Center service for non-service agreement covered DML 2000 ａnd DML 3000 units. Includes labor, parts ａnd shipping materials.    CNY            

9243652 Comprehen Valid., QuantiPlex/QP Hyres   """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   For planning purposes ａnd to ensure a smooth process, the Customer is expected to have all materials on-hand ａnd inventoried one (1) week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation ａnd generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager ａnd the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """ CNY             【人血清白蛋白(HSA)ELISA試劑盒廠家】

6000-3101   Rapid Capture System        CNY            

19134   QIAGEN Proteinase K     CNY            

614033  careHPV Test, SFDA (24-test)        CNY            

274203  flocktype IBDV Ab (5)       CNY            

270803  cattletype MAP Ab (5)       CNY            

280303  cador BVDV RT-PCR Kit (24)      CNY            

282303  virotype PRRSV RT-PCR Kit (24)      CNY            

282305  virotype PRRSV RT-PCR Kit (96)      CNY            

280443  virotype BTV pan/8 RT-PCR Kit (24)      CNY            

939012  Buffer N2 (2 x 10 ml)       CNY            

871105  EGFR PCR Kit (100)      CNY            

800382  Restriction Map     CNY            

871005  KRAS PCR Kit (100)      CNY            

QY-x2031    CoC1 卵巢癌 1640+10%胎牛血清                       

QY-x2032    CoC2 卵巢癌 1640+10%胎牛血清                       

QY-x2033    OVCAR-3 卵巢癌 DMEM+10%胎牛血清                    

QY-x2034    SK-OV-3 卵巢腺癌 1640+10%胎牛血清                       【人血清白蛋白(HSA)ELISA試劑盒廠家】

QY-x2035    SK-OV-3/DDP 卵巢腺癌順鉑耐藥株 1640+10%胎牛血清                    

QY-x2036    HeLa 宮頸癌 1640+10%胎牛血清                       

QY-x2037    HeLaS3 宮頸癌 1640+10%胎牛血清                     

QY-x2038    HEC-1B 子宮內(nèi)膜癌                      

QY-x2039    JEG-3 絨癌 1640+10%胎牛血清                    

QY-x2040    Du145 前列腺癌 高糖DMEM+10%胎牛血清                    

QY-x2041    LNCaP 前列腺癌                     

QY-x2042    LNCaP clone 前列腺癌                       

QY-x2043    PC-3 前列腺癌 DMEM+10%胎牛血清                     

QY-x2044    Tsu-pr1 非雄激素依賴(lài)型前列腺癌 1640+10%胎牛血清                    

QY-x2045    HOS 骨肉瘤 MEM+非必需氨基酸+10%胎牛血清                    

QY-x2046    MG-63 成骨肉瘤 MEM+非必需氨基酸+10%胎牛血清                    

QY-x2047    Saos-2 骨肉瘤 1640+10%胎牛血清                     

QY-x2048    U-2OS 骨肉瘤 1640+10%胎牛血清                      

QY-x2049    OS-732 骨肉瘤 1640+10%胎牛血清 瘤株                    

QY-x2050    A-204 橫紋肌肉瘤                        【人血清白蛋白(HSA)ELISA試劑盒廠家】

QY-x2051    HT1080 纖維肉瘤 1640+10%胎牛血清                       

QY-x2052    A375 皮膚黑色素瘤 DMEM+10%胎牛血清                     

QY-x2053    細(xì)胞中心實(shí)驗(yàn)室細(xì)胞庫(kù)部分細(xì)胞系目錄（鼠類(lèi)腫瘤細(xì)胞）                     

QY-x2054    1.小鼠類(lèi)                       

QY-x2055    細(xì)胞名稱(chēng) 組織類(lèi)型 培養(yǎng)條件                     

QY-x2056    Lewis 肺癌 瘤株                    

QY-x2057    LA795 肺腺癌 瘤株                      

QY-x2058    EAC 艾氏腹水瘤 瘤株                    

QY-x2059    S180 腹水瘤 瘤株                       

QY-x2060    H22 肝癌 瘤株                      

QY-x2061    H22 肝癌 1640+10%胎牛血清 體外培養(yǎng)株