產(chǎn)品展示
人神經(jīng)肽B（NKB）ELISA試劑盒廠家
點(diǎn)擊次數(shù)：11發(fā)布時(shí)間：2016/8/11 14:03:36
更新日期：2016/8/11 14:03:36
所 在 地：中國大陸
產(chǎn)品型號(hào)：
優(yōu)質(zhì)供應(yīng)
詳細(xì)內(nèi)容
【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！
本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！
操作步驟
1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品
2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘
3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘
4.洗板5次，加入顯色液A、B，37℃顯色15分鐘
5.加入終止液
6.15分鐘之內(nèi)讀OD值
7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。
【人神經(jīng)肽B（NKB）ELISA試劑盒廠家】樣本保存條件：
1.2-8℃冰箱*多放2周
2.-20到-50℃的冰箱可以放3-6個(gè)月
3.-50℃以下的冰箱，可以放一年
【人神經(jīng)肽B（NKB）ELISA試劑盒廠家】樣本收集
1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集
2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）
3.動(dòng)物組織勻漿（細(xì)胞）：
1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)
1g組織加入1ml組織裂解液加入8ml PBS 測細(xì)胞內(nèi)
糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）
沒有PBS的話也可以用一下生理鹽水，還是用PBS
【人神經(jīng)肽B（NKB）ELISA試劑盒廠家】樣本離心轉(zhuǎn)速及時(shí)間
1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清
2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清
3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。
【人神經(jīng)肽B（NKB）ELISA試劑盒廠家】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490
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QY-x1969 Panc 10.05 胰腺腺癌 高糖1640+15%胎牛血清+胰島素等
QY-x1970 SW1990 胰腺腺癌 1640+10%胎牛血清 【人神經(jīng)肽B（NKB）ELISA試劑盒廠家】
QY-x1971 JF305 胰腺癌 1640+10%胎牛血清
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QY-x1978 A2 肺腺癌 1640+10%胎牛血清
QY-x1979 A549 肺腺癌 1640+10%胎牛血清
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QY-x1981 Calu-3 肺腺癌 MEM+非必需氨基酸+10%胎牛血清
QY-x1982 GLC-82 肺腺癌 1640+10%胎牛血清 已有
QY-x1983 H1299 非小細(xì)胞肺腺癌 1640+10%胎牛血清
QY-x1984 NCI-H157 非小細(xì)胞肺腺癌 1640+10%胎牛血清
QY-x1985 NCI-H460 大細(xì)胞肺癌 1640+10%胎牛血清
QY-x1986 LTEP-P 小細(xì)胞肺癌 1640+10%胎牛血清 【人神經(jīng)肽B（NKB）ELISA試劑盒廠家】
QY-x1987 NCI-H209 小細(xì)胞肺癌 1640+10%胎牛血清
QY-x1988 NCI-H345 小細(xì)胞肺癌 1640+10%胎牛血清
QY-x1989 NCI-H446 小細(xì)胞肺癌 1640+10%胎牛血清
QY-x1990 SPC-A-1 肺腺癌 1640+10%胎牛血清
QY-x1991 Bcap-37 乳腺髓樣癌 1640+10%胎牛血清
QY-x1992 BT474 乳腺導(dǎo)管癌 DMEM+10%胎牛血清 無
QY-x1993 MDA-MB-157 乳腺癌 1640+10%胎牛血清
QY-x1994 MDA-MB-231 乳腺導(dǎo)管癌 L15+10%胎牛血清
QY-x1995 MDA-MB-453 乳腺癌 1640+10%胎牛血清
QY-x1996 RajiBurkitt 淋巴瘤 1640+10%胎牛血清
QY-x1997 RAMOSB 淋巴細(xì)胞瘤 1640+10%胎牛血清
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QY-x1999 RPMI-8226 多發(fā)骨髓瘤 1640+10%胎牛血清
