齊一生物試劑盒組分經(jīng)過重重優(yōu)化，每個(gè)組分的穩(wěn)定性都完美的經(jīng)過高溫破壞測(cè)驗(yàn)，程度的避免了長途運(yùn)輸和保存過程中抗原抗體的失活.齊一生物試劑盒憑借其完美的標(biāo)曲.嚴(yán)格的質(zhì)控.穩(wěn)定高、易保存，操作簡(jiǎn)便，已廣泛應(yīng)用在全國免疫學(xué)檢驗(yàn)研究所的各領(lǐng)域中.深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【人紅細(xì)胞生成素(EPO)抗體ELISA試劑盒現(xiàn)貨】齊一生物試劑盒憑借其完美的標(biāo)曲、嚴(yán)格的質(zhì)控、較高的穩(wěn)定性，在世界范圍內(nèi)的科研試劑領(lǐng)域中脫穎而出深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！

試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【人紅細(xì)胞生成素(EPO)抗體ELISA試劑盒現(xiàn)貨】試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

操作注意事項(xiàng)：

1.試劑盒應(yīng)按標(biāo)簽說明書儲(chǔ)存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。

2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用.請(qǐng)?jiān)谠噭┖斜Ｙ|(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長時(shí)間打開蓋子。底物B對(duì)光敏感，避免長時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。

9.按照說明書中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

【人紅細(xì)胞生成素(EPO)抗體ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

操作步驟
實(shí)驗(yàn)開始前，請(qǐng)?zhí)崆芭渲煤盟性噭�，試劑或樣品稀釋時(shí)，均需混勻，混勻時(shí)盡量避免起泡。每次檢測(cè)都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時(shí)，用樣品稀釋液進(jìn)行稀釋，以使樣品符合試劑盒的檢測(cè)范圍。
1.         加樣：分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測(cè)樣品孔�？瞻卓准訕悠废♂屢�100μl，余孔分別加標(biāo)準(zhǔn)品或待測(cè)樣品100μl，注意不要有氣泡，加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻，酶標(biāo)板加上蓋或覆膜，37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性，每次實(shí)驗(yàn)請(qǐng)使用新的標(biāo)準(zhǔn)品溶液。
2.         棄去液體，甩干，不用洗滌。每孔加生物素標(biāo)記抗體工作液 100μl（取1μl生物素標(biāo)記抗體加99μl生物素標(biāo)記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時(shí)內(nèi)配制），37℃,60分鐘。
3.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4.         每孔加辣根過氧化物酶標(biāo)記親和素工作液（同生物素標(biāo)記抗體工作液） 100μl，37℃，60分鐘。
5.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內(nèi)，此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性，底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8.         用酶聯(lián)儀在450nm波長依序測(cè)量各孔的光密度（OD值）。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測(cè)。
【人紅細(xì)胞生成素(EPO)抗體ELISA試劑盒現(xiàn)貨】注：
1. 用戶在初次使用試劑盒時(shí)，應(yīng)將各種試劑管離心數(shù)分鐘，以便試劑集中到管底。
2. 每次實(shí)驗(yàn)留一孔作為空白調(diào)零孔，該孔不加任何試劑，只是*后加底物溶液及2N H2SO4。測(cè)量時(shí)先用此孔調(diào)OD值至零。
3. 為防止樣品蒸發(fā)，試驗(yàn)時(shí)將反應(yīng)板放于鋪有濕布的密閉盒內(nèi)，酶標(biāo)板加上蓋或覆膜。
4. 未使用完的酶標(biāo)板或者試劑，請(qǐng)于2-8℃保存。標(biāo)準(zhǔn)品、生物素標(biāo)記抗體工作液、辣根過氧化物酶標(biāo)記親和素工作液請(qǐng)依據(jù)所需的量配置使用。請(qǐng)勿重復(fù)使用已稀釋過的標(biāo)準(zhǔn)品、生物素標(biāo)記抗體工作液或、辣根過氧化物酶標(biāo)記親和素工作液。
5. 建議檢測(cè)樣品時(shí)均設(shè)雙孔測(cè)定，以保證檢測(cè)結(jié)果的準(zhǔn)確性。
以上資料僅供您參考，如有問題歡迎來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9022322 Power Supply, Controller, careHPV       CNY            

9022121 Tool. Rotor Hub Height Jig, RGQ     CNY            

9021404 RGQ MDx User manuals for IVD use(Europe)        CNY            

9238610 Collet chuck        CNY            

9238479 Motor, syringe, RapidPlate      CNY            

9238152 Elution Microplate Adapter 8000, SBS        CNY            

9237932 Syringe, 100µl      CNY             【人紅細(xì)胞生成素(EPO)抗體ELISA試劑盒現(xiàn)貨】

9240663 Nut, Flangeless,1/4-28, Black, AP       CNY            

9240634 Power Supply, Main PCB, Aztec, AP196/98     CNY            

9240630 Power Supply, 5VDC 1.5 AMP, AP96        CNY            

9240593 Unload Valve, Pump, Pressure, AP196/98      CNY            

9240562 Fuse, PCB, 315, Eppendorf, AP196/98     CNY            

9240536 Motor Assy, SiNug, 17, All, AP96/196        CNY            

9240514 CD R/W, AP196/98        CNY            

9240504 PC-104, AP98        CNY            

9240346 Autopure, Full Agreement    "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9240098 Re-Installation, BR EZ1 The Re-Installation, BR EZ1 facilitates quick implementation of system back into lab routine by minimizing  instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.    CNY            

9239852 Foot bumpers,  v2.0 (4)     CNY            

9019944 Cable, P-axis, wires, 8 ch. , C18       CNY            

9238880 Bearings up. & low, BS96        CNY            

9238836 BioSprint96, Basic Agreement, no PM "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: not applicable"   CNY            

9241211 Full Agreement Depot Repair, DML2000, 1y    "The Full Agreement Depot Repair, DML2000 provides full service coverage for the DML 2000 luminometer. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."   CNY            

9241191 Magnet, tool, QX        CNY             【人紅細(xì)胞生成素(EPO)抗體ELISA試劑盒現(xiàn)貨】

9238825 Spare bucket for plate rotor        CNY            

9238815 Rect. carrier for 12 tubes 10 ml w/ cap     CNY            

9019472 TAPE SPACER CLEAR GS        CNY            

9019464 UV tube, Cxx        CNY            

9243650 Comprehen Valid., EZ1/QIAcube/QIAsymph  """The Comprehensive Validation Support plan specific to a laboratory＇s needs will incorporate all of the requirements for the internal validation guidelines from the FBI＇s Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel ａnd labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, ａnd amplify the validation study samples ａnd may require multiple individuals. Usually, two weeks is required to analyze the data ａnd one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform ａnd complete all extractions, quantifications, amplfications, ａnd capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation.

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry ａnd accessories are not included ａnd should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) ａnd required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) ａnd STR (amp) analysis ａnd linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data ａnd draft a validation summary document, ensuring compliance with the FBI＇s QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review ａnd signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

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9237333 Install, BR plus 3 integr. w. scheduling    Installation ａnd training, BioRobot System including three approved integration devices with scheduling  CNY            

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9237155 V-Housing with Sample Needle        CNY            

QY-x983 人胰腺細(xì)胞AR42J                     【人紅細(xì)胞生成素(EPO)抗體ELISA試劑盒現(xiàn)貨】

QY-x984 人胰腺癌細(xì)胞MIApaca-2                      

QY-x985 人胰腺癌細(xì)胞JF-305                     

QY-x986 人胰腺癌細(xì)胞PC-2                       

QY-x987 人胰腺癌細(xì)胞SW1990                     

QY-x988 人胰腺癌細(xì)胞CaPan-Ⅱ/Capan-2                       

QY-x989 人胰腺癌細(xì)胞CRL                    

QY-x990 人胰腺癌細(xì)胞SFPAC-1                    

QY-x991 人胚胎胰腺組織來源細(xì)胞CCC-HPE-2                    

QY-x992 人胰腺腺泡上皮癌HPAC                       

QY-x993 人胰腺腺癌細(xì)胞 Panc 10.05                      

QY-x994 人肝癌細(xì)胞HepG-2                       

QY-x995 人肝癌細(xì)胞HepG2.2.1.5                      

QY-x996 人肝癌細(xì)胞SMMC-7721                    

QY-x997 人肝癌細(xì)胞QGY-7701                     

QY-x998 人肝癌細(xì)胞QGY-7703                     

QY-x999 人肝癌細(xì)胞Bel-7402                      【人紅細(xì)胞生成素(EPO)抗體ELISA試劑盒現(xiàn)貨】

QY-x1000    人肝癌細(xì)胞Bel-7405                     

QY-x1001    人肝癌細(xì)胞Hep3B2.1-7                       

QY-x1002    人肝癌細(xì)胞SK-HEP-1                     

QY-x1003    人肝癌亞力山大細(xì)胞HCCC-9810                    

QY-x1004    人膽囊癌細(xì)胞GBC-SD                     

QY-x1005    正常人肝細(xì)胞L-02                       

QY-x1006    人胚肝細(xì)胞正常QSG-7701                     

QY-x1007    人肝細(xì)胞正常HL-7702                    

QY-x1008    CCL13張氏肝細(xì)胞正常Chang liver                     

QY-x1009    人肝星形細(xì)胞正常LX-2                       

QY-x1010    人肝內(nèi)膽管上皮細(xì)胞HIBEpiC                      

QY-x1011    人高轉(zhuǎn)移肝癌細(xì)胞LM3                    

QY-x1012    人肝癌細(xì)胞系LM-6                       

3QY-x1013   人低轉(zhuǎn)移肝癌細(xì)胞MHCC97-L