檢測標本：血清,血漿,尿液,胸腹水,腦脊液,細胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價格及詳細資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認同.大品牌保證,價格公道,傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務！本試劑盒用于測定血清,血漿及相關液體樣本中含量.

【大鼠甲狀腺球蛋白(TG)ELISA試劑盒現(xiàn)貨】實驗原理：

   本試劑盒應用雙抗體夾心法測定標本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標記的本產(chǎn)品抗體結合,形成抗體-抗原-酶標抗體復合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關.用酶標儀在450nm波長下測定吸光度（OD值）,通過標準曲線計算樣品中該產(chǎn)品濃度.

試劑盒組成：

【大鼠甲狀腺球蛋白(TG)ELISA試劑盒現(xiàn)貨】標本要求：

1．標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗.若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標準品的稀釋與加樣：在酶標包被板上設標準品孔10孔,在、第二孔中分別加標準品100μl,然后在、第二孔中加標準品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標準品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標準品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標準品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標準品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑,其余各步操作相同）、待測樣品孔.在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復5次,拍干.

6.     加酶：每孔加入酶標試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應（此時藍色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應在加終止液后15分鐘以內(nèi)進行.

【大鼠甲狀腺球蛋白(TG)ELISA試劑盒現(xiàn)貨】注意事項：

1． 試劑盒從冷藏環(huán)境中取出應在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應裝入密封袋中保存.

2． 濃洗滌液可能會有結晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結果.

3． 各步加樣均應使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差.一次加樣時間控制在5分鐘內(nèi),如標本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時做標準曲線,做復孔.如標本中待測物質(zhì)含量過高（樣本OD值大于標準品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計算時請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴格按照說明書的操作進行,試驗結果判定必須以酶標儀讀數(shù)為準.

8． 所有樣品,洗滌液和各種廢棄物都應按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準.

【大鼠甲狀腺球蛋白(TG)ELISA試劑盒現(xiàn)貨】計算：

以標準物的濃度為橫坐標,OD值為縱坐標,   

在坐標紙上繪出標準曲線,根據(jù)樣品的OD     

值由標準曲線查出相應的濃度；再乘以稀釋     

倍數(shù)；或用標準物的濃度與OD值計算出標     

準曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實際濃度.

試劑盒性能：

1.樣品線性回歸與預期濃度相關系數(shù)R值為0.95以上.

2.批內(nèi)與批見應分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

QY-x1659 小鼠腎癌細胞株RuCa                                                 

QY-x1660 大鼠肉瘤細胞WK256                                                  

QY-x1661 小鼠乳腺癌細胞MA891                                                      

2QY-x1662        大鼠胰腺癌細胞株DSL-6A/C1                                                     

QY-x1663 小鼠骨髓瘤細胞NS1                                                   

QY-x1664 大鼠卵巢癌細胞株NUTU-19                                                       

QY-x1665 小鼠腎癌細胞Ranca                                                    

QY-x1666 小鼠胚胎成纖維細胞3T3-swiss albino                                                【大鼠甲狀腺球蛋白(TG)ELISA試劑盒現(xiàn)貨】

QY-x1667 小鼠胚胎成纖維細胞3T3 clone A31                                                   

QY-x1668 小鼠胰腺腺泡細胞癌細胞MPC-83                                                     

QY-x1669 小鼠白血病細胞C3                                                      

QY-x1670 小鼠β胰島素瘤細胞RIN-m5F                                                    

QY-x1671 小鼠自發(fā)高乳腺癌細胞TA2                                               

QY-x1672 小鼠淋巴瘤細胞Nb2－11                                                   

QY-x1673 小鼠骨髓瘤細胞NS-1                                                  

QY-x1674 小鼠腎癌細胞Kert-3                                                    

QY-x1675 HPVl6 E6、E7和ras基因共轉(zhuǎn)化的C57BL／C(H-2b)小鼠肺上皮細胞TC-1                                                      

QY-x1676 長臂猿淋巴瘤MLA-144                                              

QY-x1677 細胞名稱         細胞代號         ATCC號碼        組織來源         培養(yǎng)基     ATCC描述/中科院細胞庫描述      

QY-x1678 人胚腎細胞     293T         CRL-11268?     腎     DMEM＋10％FBS            

QY-x1679 人腎上皮細胞系     293            腎     DMEM＋10％FBS            

QY-x1680 人腎上皮細胞系     293H                  腎     DMEM＋10％FBS            

QY-x1681 人臍靜脈內(nèi)皮細胞         HUVEC               臍帶靜脈         ECM或             

QY-x1682 EGM-2                                                    【大鼠甲狀腺球蛋白(TG)ELISA試劑盒現(xiàn)貨】

QY-x1683 人胚肺成纖維細胞         HLF  胚胎；肺         MEM α(+)+10%FBS                         

QY-x1684 人胚肺二倍體細胞         CCC-HPF-1         胚胎；肺         DMEM+ 10% FBS                       

QY-x1685 人微血管內(nèi)皮細胞         MVEC                 微血管     DMEM+ 10%NBCS           

QY-x1686 人支氣管平滑肌細胞     HBSMC                             RPMI 1640 + 10% NBCS           

QY-x1687 人肝癌細胞     Hep G2     HB-8065?          肝細胞癌         DMEM＋10％NBCS                  

QY-x1688 人肝癌細胞     BEL-7402          肝癌         RPMI 1640 + 10% NBCS           

QY-x1689 鼠肝癌細胞     H22  （懸�。�                   肝癌         RPMI 1640 + 10% NBCS  

9013262  Cable, 24 V, power to power, 1200 mm           CNY                             

9013249  BGR Wash System             CNY                             

9013207  Service kit, accessories, BR8000               CNY                             

9013194  Tubing, L0 outlet, auto vac,BR8000 v1.00                CNY                             

9013187  Holder, retractable tubing, dispenser, B          CNY                             

800328     Miniprep, PCR purification (Easy-Read)            CNY                             

132109     EasyXtal 15-Well DG-Tool X-Seal (100)     "100 empty 15-well crystallization plates with dropguard, X-Seal crystallization supports

For manual setup of hanging ｄrop crystallization experiments."    CNY                             

132101     NeXtal DWBlock Adhesive Foils                 CNY                             

132045     NeXtal Evolution µplate (10)             CNY                             

338172     RT2 Calibration Plate A             CNY                             

338125     384EZload Covers (4)                 CNY                             

762431     PAXgene Blood RNA MDx Kit (4)               CNY                             

203745     QIAGEN Fast Cycling PCR Kit (1000)        For 1000 x 20 µl reactions: 10 x 1 ml Fast Cycling Master Mix (2x), RNase-free water, 10 x CoralLoad Fast Cycling Dye, 5 x Q-Solution       CNY                             

203609     HotStarTaq Plus DNA Polymerase (25000)       100 x 250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 100 x 2.0ml Q-Solution, 25 mM MgCl2         CNY                             

201205     Taq DNA Polymerase (1000)    "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."         CNY                             

180850     GeneRead qPCR FAST SYBR Green/ROX MM (2)               CNY                              【大鼠甲狀腺球蛋白(TG)ELISA試劑盒現(xiàn)貨】

159300     QIAcard FTA Purification Reagent (500ml)                CNY                             

158928     Lytic Enzyme Solution (650 µl)          CNY                             

9240549  Imbalance PCB, Eppendorf, AP196/98               CNY                             

9240547  Motor, 230V, Eppendorf, AP196/98           CNY                             

9240528  Cable, Motor, Axis #5, TR, AP98                CNY                             

9240047  Thermostate unit, 120V, 1.04          CNY                             

9020029  Cable RC-3034, RGQ                  CNY                             

9020017  Oil for PM, EZ1                   CNY                             

9020016  Cable harness, VacuumStation PCB, CX1          CNY                             

9019938  Opto switch, P-axis, 1 ch. , Cx2                  CNY                             

9019486  HEPA filter, replacement cartridge, Cxx           CNY                             

9238861  BR 8000, Premium Agreement        The Premium Cover Agreement 8000 provides full service coverage for the BioRobot 8000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY                            

9238855  BR MDx, Basic Agreement      The Basic Cover Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238850  EZ1, Basic Agreement     The Basic Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.      CNY                             

9241226  Barcode Reader, EZ1a              CNY                             

9238812  Rectangular carrier, undrilled          CNY                              【大鼠甲狀腺球蛋白(TG)ELISA試劑盒現(xiàn)貨】

9019467  PLATE GLASS SHORT SET GS              CNY