1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【大鼠水通道蛋白0(AQP-0)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強(qiáng),重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【大鼠水通道蛋白0(AQP-0)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【大鼠水通道蛋白0(AQP-0)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性. 【大鼠水通道蛋白0(AQP-0)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費(fèi)代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-x1597 兔主動脈平滑肌細(xì)胞CCC-SMC-1                                                

QY-x1598 兔主動脈平滑肌細(xì)胞CCC-SMC-2                                                

QY-x1599 大鼠垂體瘤細(xì)胞MMQ                                                

QY-x1600 小鼠原B細(xì)胞株BaF3                                                 

QY-x1601 小鼠腦瘤細(xì)胞BC3H1                                                   

QY-x1602 大鼠小腸隱窩上皮細(xì)胞IEC-6                                                       【大鼠水通道蛋白0(AQP-0)ELISA試劑盒現(xiàn)貨】

QY-x1603 豬腎細(xì)胞LLC-PK1                                                 

QY-x1604 小鼠胚胎成纖維細(xì)胞3T6-Swiss albino                                               

QY-x1605 小鼠成纖維細(xì)胞C3H 10T1/2 2A6                                                

QY-x1606 小鼠樹突狀細(xì)胞肉瘤細(xì)胞DCS                                                   

QY-x1607 大鼠肝細(xì)胞IAR20                                               

QY-x1608 新生牛眼晶體上皮細(xì)胞NBLE                                                     

QY-x1609 新生牛眼Tenon＇s囊成纖維細(xì)胞NBTF                                                    

QY-x1610 兔角膜后基質(zhì)層成纖維細(xì)胞RCBBF                                                   

QY-x1611 兔角膜前基質(zhì)層成纖維細(xì)胞RCFBF                                                    

QY-x1612 大鼠氣管上皮細(xì)胞RTE                                               

QY-x1613 兔眼Tenon＇s囊成纖維細(xì)胞RYTF                                                     

QY-x1614 小鼠乳腺癌細(xì)胞CCC-Ca761-03                                                   

QY-x1615 果子貍腎上皮細(xì)胞Hed68                                                   

QY-x1616 大鼠骨骼肌成肌細(xì)胞L6                                                      

QY-x1617 小鼠肺腺癌細(xì)胞系LA795                                                    

QY-x1618 鼬肺上皮細(xì)胞MV-1-Lu                                                 【大鼠水通道蛋白0(AQP-0)ELISA試劑盒現(xiàn)貨】

QY-x1619 兔晶體上皮細(xì)胞永生系N/N1003A（RLE）                                                       

QY-x1620 大鼠嗜堿性粒細(xì)胞性白血病細(xì)胞RBL-1                                                     

QY-x1621 小鼠B淋巴細(xì)胞雜交瘤細(xì)胞SH2                                               

QY-x1622 小鼠B淋巴細(xì)胞雜交瘤細(xì)胞SH3                                               

QY-x1623 倉鼠/小鼠雜交瘤細(xì)胞145-2C11                                                 

QY-x1624 小鼠T淋巴細(xì)胞Cl.Ly1+2-/9                                                

QY-x1625 大鼠骨髓瘤細(xì)胞IR983F                                                       

QY-x1626 小鼠單核巨噬細(xì)胞J774A.1                                                 

QY-x1627 小鼠腎集合管細(xì)胞(SV40轉(zhuǎn)化)M-1                                                     

9022748  Actuator, Tip Eject, DCU          CNY                             

9022733  RQ document, RGQ          CNY                              【大鼠水通道蛋白0(AQP-0)ELISA試劑盒現(xiàn)貨】

9022708  Baffle, PMT, DML3000              CNY                             

9016065  Maintenance Kit, Basic, BR8000 I            CNY                             

9016063  Tubing Set, Waste DISU, BR8000              CNY                             

9015724  Probe, 0.9 mm, BR8000 v1.00                   CNY                             

9015534  Cover, front, type B          CNY                             

9015529  Filter, inlet, with switch & fuse                  CNY                             

9014202  Tubing Sys., Bottle, BR8 RD              CNY                             

9021194  Grid, fan, SP, Qsym            CNY                             

9021128  Cooling adapter, PCR 384, Qsym               CNY                             

9021101  Skin Panel, Lower Left Rear, DCU             CNY                             

9238279  PAXgene 96 Incubator Block (farbl.elox)          CNY                             

9240677  Bumper, Round, Latch Lock, AP98            CNY                             

9240635  Filter, EMI,Modular, RJ45, AP196/98                 CNY                             

9240615  Fuse, 1.0A, FNM, AP96             CNY                             

9240612  Turk Sensor, Waste Bottle Level, AP                  CNY                             

9240603  Deck/Waste Overflow Sensor, AP196/98           CNY                              【大鼠水通道蛋白0(AQP-0)ELISA試劑盒現(xiàn)貨】

9240583  Regulator, Pressure, Main, AP                   CNY                             

9240545  Hatch pad, Eppendorf, AP196/98               CNY                             

9240542  Lower Header, Capper Head, AP196                 CNY                             

9240521  Motor Assy, Shaker, AP98                  CNY                             

9240520  Motor Assy, Sz. 17, Common, AP98                   CNY                             

9240102  Re-Installation, LiquiChip          The Re-Installation, LiquiChip facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware ａnd software check of instrument ａnd provides user with operator refesher overview.  Re-installation includes labor ａnd travel costs. Re-installation performance process is documented on a GMP/GLP-compliant Field Service document.          CNY                             

9019940  Power supply, vac pump, CX1           CNY                             

9019477  LASER MODUAL GS          CNY                             

9019476  Spacer, 0.2 mm short set, GSC                   CNY                             

9238888  PCB, Main-01, BS96                   CNY                             

9238886  Cable, HM, BS96               CNY                             

9238854  #BR M96, Basic Agreement    The Basic Cover Agreement M96 provides full service coverage for the BioRobot M96. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238842  RS232 box, non-online fill, RapidPlate               CNY                             

9241215  Full Agreement Depot Repair, Shaker, 1yr       "The Full Agreement Depot Repair, Shaker provides full service coverage for the plate shaker used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."          CNY                             

9238833  #BR 9604, Basic Agreement    The Basic Cover Agreement 9604, 1 PM provides full service coverage for the BioRobot 9604. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY