1.購買我公司任意ELISA試劑盒指標(biāo)享受免費(fèi)代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實(shí)驗(yàn)材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時(shí)備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠水通道蛋白4(AQP-4)ELISA試劑盒廠家】三. 仔細(xì)閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請(qǐng)勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個(gè)復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實(shí)驗(yàn)者，注意低溫保存

5.準(zhǔn)備好所有實(shí)驗(yàn)額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲(chǔ)。 【大鼠水通道蛋白4(AQP-4)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計(jì)時(shí)就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時(shí)間和溫度。

10.切勿使用不同生產(chǎn)批號(hào)的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時(shí)，避免泡沫產(chǎn)生。

12.在實(shí)驗(yàn)開始之前，安排好實(shí)驗(yàn)流程。在實(shí)驗(yàn)開始之前，清潔工作臺(tái)。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【大鼠水通道蛋白4(AQP-4)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對(duì)收集后當(dāng)天就進(jìn)行檢測的標(biāo)本，及時(shí)儲(chǔ)存在4℃?zhèn)溆�，如有特殊原因需要周期收集�?biāo)本，請(qǐng)?jiān)炷Ｈ〔暮�，將�?biāo)本及時(shí)分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實(shí)驗(yàn)質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項(xiàng)請(qǐng)與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等。

4.購買前請(qǐng)?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【大鼠水通道蛋白4(AQP-4)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細(xì)內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實(shí)驗(yàn)具體詳細(xì)步驟；

3、完整的實(shí)驗(yàn)報(bào)告（試劑耗材，實(shí)驗(yàn)方法，實(shí)驗(yàn)結(jié)果及結(jié)果說明）；

4、實(shí)驗(yàn)進(jìn)行階段，我公司客服人員會(huì)及時(shí)向您匯報(bào)實(shí)驗(yàn)進(jìn)程。

QY-x1504 中國倉鼠倉鼠肺細(xì)胞R1610                                               

QY-x1505 綠猴猴脈絡(luò)膜-視網(wǎng)膜(內(nèi)皮)細(xì)胞RF/6A                                                     

QY-x1506 大鼠雪旺細(xì)胞RSC96                                                     【大鼠水通道蛋白4(AQP-4)ELISA試劑盒廠家】

QY-x1507 豬睪丸細(xì)胞系(ST)ST                                                     

QY-x1508 蝙蝠肺細(xì)胞Tb 1 Lu                                                       

QY-x1509 小鼠B細(xì)胞雜交瘤細(xì)胞W6/32                                                    

QY-x1510 鼠纖維肉瘤細(xì)胞系WEHI 164                                                      

QY-x1511 小鼠B淋巴細(xì)胞WEHI 231                                                 

QY-x1512 大鼠垂體細(xì)胞RPC                                               

QY-x1513 小鼠垂體細(xì)胞MPC                                                       

QY-x1514 兔子垂體細(xì)胞                                                       

QY-x1515 狗垂體細(xì)胞                                                  

QY-x1516 鼠肝癌細(xì)胞系MM45T.Li                                                      

QY-x1517 小鼠成纖維細(xì)胞系McCoy                                                   

QY-x1518 大鼠肝癌細(xì)胞（乳腺癌細(xì)胞接種到肝臟成瘤）Walker-256                                                    

QY-x1519 大鼠肝癌細(xì)胞（wistar 大鼠）CBRH7919                                                 

QY-x1520 小鼠結(jié)腸癌C26                                                    

QY-x1521 穩(wěn)定轉(zhuǎn)染了neor和LIF基因的STO細(xì)胞株SNL                                                

QY-x1522 小鼠艾氏腹水瘤細(xì)胞ECA                                                    【大鼠水通道蛋白4(AQP-4)ELISA試劑盒廠家】

QY-x1523 褐鼠胰島素瘤上皮細(xì)胞RIN-m                                                   

QY-x1524 小鼠雜交瘤細(xì)胞ST2/o                                                 

QY-x1525 小鼠腎癌細(xì)胞RenCa                                                   

QY-x1526 小鼠胰島素瘤β細(xì)胞（NOD/Lt轉(zhuǎn)基因小鼠，SV40巨T抗原）NIT-1                                                    

QY-x1527 早代小鼠胚胎成纖維細(xì)胞MEF                                                  

QY-x1528 中國倉鼠肺細(xì)胞V79                                                   

QY-x1529 兔的肝細(xì)胞VX2                                                   

QY-x1530 雞胚原代肝細(xì)胞CEL                                                    

QY-x1531 129小鼠ES細(xì)胞D3                                                     

QY-x1532 129小鼠ES細(xì)胞E14                                                   

QY-x1533 BALB/c小鼠ES細(xì)胞B/c-ES                                                 

QY-x1534 猴的骨髓間充質(zhì)干細(xì)胞MSCS                                                     

9241373  Cable, key panel board, EZ1a           CNY                             

9240752  Downtube, Reagent #2-6, AP98               CNY                             

9240751  Belt, TR,Capper U/D & I/O, AP                  CNY                             

9243546  QIAsymphony SP AS, PM Service      "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9243312  Differential Wash Protocol Pack, QIAcube                CNY                              【大鼠水通道蛋白4(AQP-4)ELISA試劑盒廠家】

9242763  QS RGQ, Full Agreement "Repair service delivery QIAsymphony SP/AS: onsite

Service response time (repair) QIAsymphony: 48 hours/2 business days

Repair service delivery RGQ: send to Regional Repair Center

Service response time (repair) RGQ: 7-10 days turnaround time

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision RGQ: yes, included (provision time 2-3 days)

Transportation cost coverage: yes, full coverage"  CNY                             

9242547  SDS Canbus Cable, LC                 CNY                             

5000-1289        DML 2000 - Power Cord - 220V Switzerland             CNY                             

5000-1286        Fuse 2 AMP PKD 10, DML2                CNY                             

5000-1264        Cable Modem INST 9 PIN, DML2              CNY                             

5000-1507        Microplate Heater I - Fuse Inlet 3A 250V                  CNY                             

6000-00180      Plate Washer - Rinse Cap Assembly          CNY                             

6000-00179      Plate Washer - Wash Cap Assembly                   CNY                             

5000-1424        Brush Motor 2 Required, VTX2                  CNY                             

5000-1421        Block Support Back 099, VTX2                   CNY                             

5080-1000        Screw Caps                CNY                             

PUREGST2        GST-tag Purification ａnd Detection Kit 2                   CNY                             

5000-1411        Poteneiometer 100L OHM, VTX2             CNY                             

6000-5026        hc2 Sample Conversion Tubes 15mL                  CNY                             

L2127       hc2 High-Risk HPV DNA Test PI (CD-ROM)                CNY                             

12943       QIAGEN Plasmid Plus Midi Kit (25)  "25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."    CNY                              【大鼠水通道蛋白4(AQP-4)ELISA試劑盒廠家】

9240710  Filter, Pump, Jun-Air, Inlet/Outlet, AP               CNY                             

9240709  Filter, Pressure, 5 Micron, AP196/98                  CNY                             

9242094  QIAcube, Inspection Agreement     "Repair service delivery: not applicable

Service response time (repair): not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for repair parts: yes, for Periodic Inspection parts

Cost coverage for labor: yes, included for Periodic Maintenance

Cost coverage for travel: yes, included for Periodic Maintenance

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"        CNY                             

9242095  QIAxcel, Inspection Agreement                CNY                             

9241331  #QIAxcel, Full Agreement, no PM   The Full Agreement QIAxcel covers all travel, labor ａnd repair part costs related to an instrument repair for 1 year. Service response time is 2 working days.  CNY                             

9001772  careHPV test System                 CNY                             

275703     flocktype Salmonella Ab (5)               CNY                             

270045     cattletype BHV1 gB Ab (20)              CNY                             

274013     flocktype AIV Ab (5)          CNY                             

289993     virotype Tissue Lysis Reagent (250 ml)              CNY                             

281807     virotype CSFV RT-PCR Kit (480)                  CNY                             

273403     pigtype Toxoplasma Ab (5)                CNY