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1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測測定的精度和提高檢測結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個試劑盒采用stripwell微孔板。這允許用戶在不同的時間來分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個工具都有一個包含一個典型數(shù)據(jù)的圖表的手冊。在運(yùn)營商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應(yīng)該如何儲存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長期儲存，建議凍結(jié)他們在- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會給出錯誤的結(jié)果。

7。我可以使用一個不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗證在試劑盒插入的樣品類型。未經(jīng)檢驗的樣品類型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動態(tài)范圍內(nèi)的測定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測的。

9。我每次都要運(yùn)行一個空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當(dāng)排除特定的檢測問題的來源時，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個組件包含有特定批號的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對這些特定的地段進(jìn)行質(zhì)量檢驗。這是從來沒有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來很好，但我沒有得到一個信號，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋，請檢查是否正確進(jìn)行稀釋。過稀釋會導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個自動平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動洗衣機(jī)也是一樣的。一個中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無塵紙。【大鼠水通道蛋白5(AQP-5)ELISA試劑盒現(xiàn)貨】

14。我需要用一個盤子嗎？

可靠的結(jié)果可以得到無板振動，但外徑的一般會低于那些使用平板振動器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯(lián)免疫吸附試驗，在雙波長讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細(xì)胞來源。這個信息的*佳來源是目前的文獻(xiàn)，很容易通過互聯(lián)網(wǎng)在多個科學(xué)數(shù)據(jù)庫訪問。

18。我的光密度有點高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【大鼠水通道蛋白5(AQP-5)ELISA試劑盒現(xiàn)貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【大鼠水通道蛋白5(AQP-5)ELISA試劑盒現(xiàn)貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【大鼠水通道蛋白5(AQP-5)ELISA試劑盒現(xiàn)貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

QY-x1473 化學(xué)轉(zhuǎn)化小鼠鼻咽上皮細(xì)胞TMNE                                                    

QY-x1474 大鼠鼻咽癌細(xì)胞FAT                                                     【大鼠水通道蛋白5(AQP-5)ELISA試劑盒現(xiàn)貨】

QY-x1475 小鼠大腸癌細(xì)胞CT-26.WT                                                  

QY-x1476 小鼠結(jié)腸癌細(xì)胞CMT93                                                       

QY-x1477 大鼠埀體瘤細(xì)胞GH3                                                  

QY-x1478 產(chǎn)EBV的絨猴白細(xì)胞B95-8                                               

QY-x1479 小鼠艾氏腹水瘤細(xì)胞TCM15S180                                                       

QY-x1480 小鼠胚胎成纖維細(xì)胞T6-Swiss albino                                                 

QY-x1481 小鼠皮下結(jié)締組織細(xì)胞A9                                                 

QY-x1482 蚊子細(xì)胞C6/36                                                      

QY-x1483 鼠小腦細(xì)胞C8-D1A                                                      

QY-x1484 貓腎細(xì)胞CRFK                                                      

QY-x1485 貓腎細(xì)胞F81                                               

QY-x1486 中國倉鼠卵巢細(xì)胞CTLA4 Ig-24                                                  

QY-x1487 非洲綠猴非洲綠猴腎細(xì)胞CV-1                                                  

QY-x1488 牛胚氣管細(xì)胞EBTr (NBL-4)                                                 

QY-x1489 恒河猴胚腎細(xì)胞FRhK-4                                                       

QY-x1490 大鼠肝癌細(xì)胞H4-ⅡE                                                   【大鼠水通道蛋白5(AQP-5)ELISA試劑盒現(xiàn)貨】

QY-x1491 大鼠肝細(xì)胞瘤H4-II-E-C3                                                      

QY-x1492 大鼠心肌細(xì)胞H9c2(2-1)                                                      

QY-x1493 大鼠腎小球系膜細(xì)胞EC (HBZY－1)HBZY－1                                                     

QY-x1494 綠猴恒河猴腎細(xì)胞LLC-MK2                                               

QY-x1495 牛腎細(xì)胞MDBK (NBL-1)                                                       

QY-x1496 貂肺上皮細(xì)胞Mv.1.Lu(NBL-7)                                                     

QY-x1497 小鼠腦神經(jīng)瘤細(xì)胞Neuro-2a [N2a; Neuro-2a]                                                    

QY-x1498 大鼠肺泡巨噬細(xì)胞NR8383                                                

QY-x1499 負(fù)鼠腎細(xì)胞OK                                                     

QY-x1500 小鼠骨髓瘤細(xì)胞P3/NSI/1-Ag4-1 [NS-1]                                              

QY-x1501 小鼠骨髓瘤細(xì)胞P3X63Ag8.653                                                  

QY-x1502 豬髖動脈內(nèi)皮細(xì)胞PIEC                                                       

QY-x1503 鼠逆轉(zhuǎn)錄酶病毒包裝細(xì)胞PT67                                                 

6000-3116        Plate Washer I - 105 Micron Filters          CNY                             

6000-00183      Plate Washer - Manifold           CNY                             

6000-00177      Plate Washer - Syringe Assembly              CNY                             

5000-1501        Holder Fuse, HTR1            CNY                             

5135-1220        hc2 CT-ID DNA Test           CNY                             

BR-01       DxS B-RAF Mutation Test Kit            CNY                              【大鼠水通道蛋白5(AQP-5)ELISA試劑盒現(xiàn)貨】

9240716  Lead Screw Assy, Capper U\D, AP196/98          CNY                             

9240712  Muffler, Elevator Brake, AP196/98            CNY                             

5000-1416        Switch LRGSK61RCBE250, VTX2                CNY                             

6000-5023        LumiCheck Plate User Package (CD Manual)            CNY                             

6000-3102        Rapid Capture System & Manuals            CNY                             

12941       QIAGEN Plasmid Plus Midi Sample Kit (5)         QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.      CNY                             

9240695  Needle Valve, Flow Control, AP196/98               CNY                             

9002141  careHPV test Shaker                  CNY                             

273501     pigtype Trichinella Ab (1)          CNY                             

273803     pigtype Yersinia Ab (5)               CNY                             

280433     virotype BTV RT-PCR Kit (24)            CNY                             

281607     virotype SBV RT-PCR Kit (480)          CNY                             

800371     Shipping,Postage,Packaging (stand. mail)                 CNY                             

9019497  ZLV MANUAL CAS1200              CNY                             

9020112  Accessory power lead (vac. station), QXT                  CNY                             

9020578  Adapter, USB to Serial, DML 3000            CNY                              【大鼠水通道蛋白5(AQP-5)ELISA試劑盒現(xiàn)貨】

9020587  Pulley, motor, DML 3000          CNY                             

9020595  Rubber Foot, 2" Dia x 1" Ht, DML 3000           CNY                             

9232722  Cooling block, 96-tube 0.2 ml  Holder for accommodating 96-well amplification plate (0.2 ml per well) on the worktable         CNY                             

9013265  Cable, 24V, power to cooling, 1900 mm           CNY                             

9013257  Tubing set , HSPS, 90/120,0               CNY                             

9013250  BGR Waste System           CNY                             

9013216  Screws, plastic, M4 (20)            CNY                             

9013202  Valve set, throttle, auto vac, BR8000               CNY                             

9011758  Reagent holder, 8-tube 1.5 ml         Holder for accommodating 8 microcentrifuge tubes (1.5 ml) on the worktable; must be used with the reagent holder tray  CNY                             

132625     NeXtal Stock TRIS. HCl (50)      1M Tris(hydroxymethyl)aminomethane HCl - 50mL (4 x 12,5 ml tube)   CNY                             

9241374  Magnet, door lock, bottom, EZ1a            CNY