【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準(zhǔn)確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個完整的計劃。

【大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

QY-x1203       人高轉(zhuǎn)移卵巢癌細(xì)胞HO-8910PM                                        

QY-x1204       人卵巢癌細(xì)胞8910PM                                   

QY-x1205       人卵巢癌細(xì)胞HOC1                                       

QY-x1206       人卵巢癌細(xì)胞COC1                                       

QY-x1207       人卵巢癌細(xì)胞OVAC                                       

QY-x1208       人卵巢癌細(xì)胞Ovary                                       

QY-x1209       人卵巢畸胎瘤細(xì)胞PA-1                                        

QY-x1210       人卵巢癌細(xì)胞SK-OV-3                                          【大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)ELISA試劑盒現(xiàn)貨】

QY-x1211       人卵巢透明細(xì)胞癌細(xì)胞ES-2                                        

QY-x1212       人卵巢癌細(xì)胞系3AO                                     

QY-x1213       人子宮內(nèi)膜癌細(xì)胞ISK （Ishikawa）                                         

QY-x1214       人子宮內(nèi)膜腺癌細(xì)胞HEC-1-A                                      

QY-x1215       人子宮內(nèi)膜腺癌細(xì)胞HEC-1-B                                      

QY-x1216       人子宮內(nèi)膜腺癌細(xì)胞AN3 CA                                       

QY-x1217       人子宮內(nèi)膜腺癌細(xì)胞KLE                                      

QY-x1218       人宮頸癌細(xì)胞HeLa                                        

QY-x1219       人Hela細(xì)胞耐藥亞株(Hela/DDP)Hela                                    

QY-x1220       人宮頸癌細(xì)胞(HPV-18 永生化細(xì)胞)HeLa229                                      

QY-x1221       人宮頸癌細(xì)胞Hela P10s-11F                                         

QY-x1222       人宮頸癌細(xì)胞HeLa-S3                                   

QY-x1223       人宮頸癌細(xì)胞SiHa                                         

QY-x1224       宮頸癌細(xì)胞HCE1                                    

QY-x1225       人宮頸癌細(xì)胞CaSKi                                       

QY-x1226       人子宮內(nèi)膜癌細(xì)胞RL95-2                                      【大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)ELISA試劑盒現(xiàn)貨】

QY-x1227       人子宮鱗癌細(xì)胞（高分化）HCC 94                                     

QY-x1228       人子宮頸癌細(xì)胞C-33A                                   

QY-x1229       人前列腺癌細(xì)胞PC-3                                     

QY-x1230       人前列腺癌細(xì)胞PC-3M                                         

QY-x1231       人前列腺癌細(xì)胞高轉(zhuǎn)移亞系PC-3M 1E8                                      

QY-x1232       人前列腺癌細(xì)胞高轉(zhuǎn)移亞系PC-3M 2B4                                      

QY-x1233       人前列腺癌細(xì)胞DU-145                                        

9236417  Software, Twister I, TWI            CNY                           

9236264  Elution Microtubes Adaptor, inject mold              CNY                           

9236165  O-ring, lubrication kit, RapidPlate         CNY                           

9235931  Splashing guard, BR8 RD             CNY                           

9230822  Rack for reagents,Trough (for 6 troughs)           CNY                           

9235707  Washer Cable, RCS1             CNY                           

9235695  Ground Wire, RCS1              CNY                           

9235332  Z-Rack Packing Cover          CNY                           

9235118  Fuse F1A, v1.00           CNY                           

9235114  Fuse FST4A, v1.00              CNY                           

9230054  Clamp, Tubing, Z-bar           CNY                           

9234861  Lid coupling piece for system liquid             CNY                           

9239551  Cbl Assy, XY, LED Driver            CNY                           

9238663  Hook, motorized lid lock              CNY                           

9238642  Capacitor board             CNY                           

9238626  Opto-Encoder        CNY                            【大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)ELISA試劑盒現(xiàn)貨】

9238625  Eprom ａnd controller 4K15, Zent3C            CNY                           

9241828  Pyro Q96 MDa, Premium Agreement    "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"      CNY                           

9241122  Superlube              CNY                           

9241007  QIArent&go - Rent out fee QIAcube           CNY                           

9240985  PCB, drive, cooling device, Qsym        CNY                           

9240982  Sensor, x, sample tracking, Qsym        CNY                           

9240951  Support, cable, portal, Qsym        CNY                           

9234820  Manual, user, QS 4.0, Int             CNY                           

9240768  Signal Conditioner, Centrifuge, AP96           CNY                           

9240764  DC Output Module 8NPT, 3AMP, AP96              CNY                           

6000-5003      Duraseal Sealing Film            CNY                           

9241781  RGQ, Full Agreement, no PM       "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included"       CNY                           

9241776  QIAgility, Inspection Service "The Annual Inspection Service, QIAgility is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAgility are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report." CNY                           

9241756  IQ/OQ Services, QIAsymphony    Instrument qualification support service for the QIAsymphony. The product contains the Installation Qualification (IQ)/Operation Qualification (OQ) documentation ａnd the performance of the qualification protocols (travel ａnd labor costs included).      CNY                           

9237126  Upgrade App., PCR Setup            CNY                           

9237093  Training, Extension day  Additional days of training may be purchased during the week of the site visit upon request.       CNY                            【大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)ELISA試劑盒現(xiàn)貨】

9236426  Board, arm interconnect, TWI             CNY