檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【大鼠αL巖藻糖苷酶(AFU)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【大鼠αL巖藻糖苷酶(AFU)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【大鼠αL巖藻糖苷酶(AFU)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【大鼠αL巖藻糖苷酶(AFU)ELISA試劑盒現(xiàn)貨】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

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QY-x643   Hep3B肝癌細(xì)胞                                                   

QY-x644   MG-63成骨肉瘤細(xì)胞                                                  

QY-x645   Hep G2肝癌細(xì)胞                                                 

QY-x646   Saos-2骨肉瘤細(xì)胞                                               

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QY-x648   U-2 OS骨肉瘤細(xì)胞                                                       

QY-x649   PAN-1胰腺導(dǎo)管上皮癌細(xì)胞                                               

QY-x650   OS-732骨肉瘤細(xì)胞（瘤株）                                                       

QY-x651   Panc 10.05胰腺腺癌細(xì)胞                                                    

QY-x652   A-204橫紋肌肉瘤細(xì)胞                                                

QY-x653   SW1990胰腺腺癌細(xì)胞                                                

QY-x654   HT1080纖維肉瘤細(xì)胞                                                 

QY-x655   SK-RC-42腎癌細(xì)胞                                               

QY-x656   A375皮膚黑色素瘤細(xì)胞                                                      

QY-x657   RCC-krause腎癌細(xì)胞                                                    

QY-x658   A875黑色素瘤細(xì)胞                                                      

QY-x659   Ketr-3腎癌（瘤株）                                                    

QY-x660   SK-HEL-1皮膚黑色素瘤細(xì)胞                                               

QY-x661   SW 13腎上腺皮質(zhì)瘤細(xì)胞                                                    

QY-x662   A431皮膚基底細(xì)胞癌細(xì)胞                                                 

QY-x663   96-C低轉(zhuǎn)移肺癌細(xì)胞                                                   【大鼠αL巖藻糖苷酶(AFU)ELISA試劑盒現(xiàn)貨】

QY-x664   CEM白血病細(xì)胞                                                  

QY-x665   95-D高轉(zhuǎn)移肺癌細(xì)胞                                                  

QY-x666   K562慢性髓原白血病細(xì)胞                                                 

QY-x667   973肺腺癌細(xì)胞                                                    

QY-x668   HPB-ALLT細(xì)胞白血病細(xì)胞                                                  

QY-x669   A2肺腺癌細(xì)胞                                                      

QY-x670   HL-60白血病細(xì)胞                                                

QY-x671   A549肺腺癌細(xì)胞                                                 

QY-x672   DaudiB淋巴細(xì)胞瘤細(xì)胞                                                      

9019335  Transistor, power PCB, heater supply, RG                 CNY                             

9234678  Reader Power Supply Board             CNY                              【大鼠αL巖藻糖苷酶(AFU)ELISA試劑盒現(xiàn)貨】

1027280  AllStars Negative Control siRNA (5 nmol)                  CNY                             

1027271  miScript Negative Control Inhibitor(5)              CNY                             

1022076  Control (non-sil.) siRNA (5 nm)                   CNY                             

9019225  Valve - Release, PyroQ24                   CNY                             

9019223  Power Supply - Cooling, PyroQ24              CNY                             

9019221  Cooling module, PyroQ24                  CNY                             

1061063  CT PCR REAGENT II           CNY                             

1061064  CT Quantification Standards             CNY                             

1061103  HCMV PCR REAGENT I              CNY                             

1061105  MP PCR REAGENT II                  CNY                             

1061110  NG PCR REAGENT I           CNY                             

1061123  HBV PCR REAGENT V                CNY                             

9016386  EZ1 VRS Card            CNY                             

9016234  BGR Zentrieradapter                CNY                             

9019888  Lid, label 2CH, RG3           CNY                             

9019877  Cover, main, blue, RG6             CNY                              【大鼠αL巖藻糖苷酶(AFU)ELISA試劑盒現(xiàn)貨】

9020805  Single LED, blue, Qsym              CNY                             

9013538  Underlay, cooling block             CNY                             

9013407  Tubing set, without PVC tubing, BR9604                   CNY                             

9238802  Sealing cap, clear, incl. clips              CNY                             

9019625  PCB, level sensor rev8, Cx2                CNY                             

9019579  LED, power assembly, C1x                 CNY                             

9019558  Pulley,  X idler, Al15T2.5/40-2, Cxx          CNY                             

9019545  Cable, RS232, 9 pin, 2m, Cxx             CNY                             

9019540  Kit, screws & washers, inst. covers, Cxx           CNY                             

9019512  Tube, pinch valve, CX1               CNY                             

9019503  TANK LOWER BUFFER ASSY GS                  CNY                             

217061     miRNeasy 96 Kit (4)          CNY                             

204257     QuantiFast Probe PCR Kit (4000)               CNY                             

9019391  Fan, cooler blower, RG3           CNY                             

9019384  Board, main, dual & OEM, RG3                CNY                             

204141     QuantiTect SYBR Green PCR Kit (40)        "Trial kit size for 40 x 50 ul reactions: 1.7 ml QuantiTect SYBR Green PCR Master Mix, 2.0 ml RNase-free water

For quantitative, real-time PCR ａnd two-step RT-PCR using SYBR Green detection."    CNY                             

9019428  Sensor, source home, RG3                 CNY                            

9019397  Belt, toothed, rotor drive, RG3                 CNY                             

9019350  Heater assembly, element, sensors, RG6                  CNY                             

1027415  FlexiTube siRNA 1 nmol             CNY                             

9019336  Connector, JP5, Hi, Med, Lo, RG6             CNY