【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準備試劑，樣品和標準品

2.加入準備好的樣品和標準品，37℃反應30分鐘

3.洗板5次，加入酶標試劑，37℃反應30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質量控制和全過程質量控制。在收集標本前都必須有一個完整的計劃。

【大鼠磷酸肌醇3激酶(PI3K)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【大鼠磷酸肌醇3激酶(PI3K)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【大鼠磷酸肌醇3激酶(PI3K)ELISA試劑盒現(xiàn)貨】樣本離心轉速及時間

1.試管采集的樣本：3000轉，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應將其分成小部分-70 ℃保存，避免反復冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應在室溫下解凍并確保樣品均勻地充分解凍。

【大鼠磷酸肌醇3激酶(PI3K)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

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QY-x186  NIH/3T3胚胎成纖維細胞    CRL-1658                                 

QY-x187  MC3T3-E1 Subclone 14小鼠原成骨細胞   CRL-2594                                 

QY-x188  L6大鼠成肌細胞         CRL-1458                                 

QY-x189  H9c22-1大鼠心肌細胞        CRL-1446                                 

QY-x190  RSC96大鼠雪旺細胞   CRL-2765                                  【大鼠磷酸肌醇3激酶(PI3K)ELISA試劑盒現(xiàn)貨】

QY-x191  CHO/dhFr-中國倉鼠二氫葉酸還原酶缺陷細胞  CRL-9096                                 

QY-x192  Lec1卵巢細胞      CRL-1735                                 

QY-x193  293T/17 [HEK 293T/17] 人胚腎細胞  CRL-11268                                

QY-x194  AAV-293 胚腎細胞                                        

QY-x195  hFOB 1.19SV40 轉染成骨細胞   CRL-11372                                

QY-x196  CCD-1095Sk皮膚細胞        CRL-2122                                 

QY-x197  Hs 578Bst乳腺細胞      HTB-125                                   

QY-x198  NK-92人惡性非霍奇金淋巴瘤患者的自然殺傷細胞       CRL-2407                                 

QY-x199  NK-92MI人惡性非霍奇金淋巴瘤患者的自然殺傷細胞   CRL-2408                                 

QY-x200  HMy2.CIR人B 淋巴母細胞       CRL-1993                                 

QY-x201  OK負鼠opossum腎細胞     CRL-1840                                 

QY-x202  FO骨髓瘤細胞     CRL-1646                                 

QY-x203  RAW 264.7單核巨噬細胞白血病       TIB-71                               

QY-x204  P19畸胎癌細胞    CRL-1825                                 

QY-x205  C6膠質瘤細胞      CCL-107                                   

QY-x206  RBL-2H3白血病細胞    CRL-2256                                  【大鼠磷酸肌醇3激酶(PI3K)ELISA試劑盒現(xiàn)貨】

QY-x207  SiHa子宮頸癌細胞       HTB-35                              

QY-x208  MG-63骨肉瘤細胞       CRL-1427                                 

QY-x209  HEC-1-B子宮內(nèi)膜腺癌細胞        HTB-113                                   

QY-x210  SW1353骨肉瘤細胞     HTB-94                              

QY-x211  ES-2卵巢透明細胞癌細胞   CRL-1978                                 

QY-x212  Saos-2成骨肉瘤細胞    HTB-85                              

QY-x213  MDA-MB-468乳腺癌細胞   HTB-132                                   

QY-x214  SK-MES-1肺鱗癌細胞        HTB-58                              

QY-x215  MDA-MB-435S乳腺癌細胞        HTB-129                                   

9020997  ZS Pipetting Module, DCU           CNY                           

9020909  Cable, UV curly cord, SMC, QGX              CNY                            【大鼠磷酸肌醇3激酶(PI3K)ELISA試劑盒現(xiàn)貨】

9020908  Hinge, lid, SMC, QGX          CNY                           

9020643  Waste chute, straight, AS, Cabinet Qsym            CNY                           

9020632  Kit, hinges (4), Cabinet Qsym              CNY                           

9020629  Door, waste, SP, Cabinet Qsym           CNY                           

9020625  Kit, repair, Cabinet Qsym             CNY                           

9020332  EPROM - Motherboard, RGQ              CNY                           

9020233  Foot assembly, rear, CAS             CNY                           

9020158  Insulation disc - chamber bottom, RG3        CNY                           

9020096  Locking Pin, TLyser 2          CNY                           

9020085  Thrust Washer, TLyser         CNY                           

9239498  V-Housing, LX100         CNY                           

9239490  EX Laser Driver, Uniphase           CNY                           

9239390  PCR Plate Adapter, M96        CNY                           

9236510  BR 3000, PM Service    The Overhaul covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed. CNY                           

9232764  Disposable Troughs, 20 / 30 ml (10)    Troughs holding up to 20 ml of liquid; pack of 10       CNY                           

9236472  BR 9600, Full Agreement       The Full Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).       CNY                            【大鼠磷酸肌醇3激酶(PI3K)ELISA試劑盒現(xiàn)貨】

9236443  BR, Installation       The Installation, BR Universal facilitates the quick implementation of the BioRobot into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the BioRobot Universal instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9239155  Assembly, vertical axis, TWI        CNY                           

9239108  Assy, Sample Needle, Actuator linkage         CNY                           

9232693  MTP-Plate Holder  2.01              CNY                           

9232629  Bottle, 500-ml, yellow fitting         CNY                           

9232602  Vacuum manifold, plate holder bottomless           CNY                           

9232579  Cooling block, 1,5ml             CNY                           

9238951  Locking lever, BS15              CNY                           

9238798  Rectangular bucket, sealable with cap          CNY                           

9238794  Angle rotor 6 x 250 ml          CNY                           

9016913  Tubing, press relief valve inlet DISU v2              CNY                           

9238942  Keyboard-Display assembly, BS15        CNY                           

9238753  Screwdriver, hex2mm, z-drive BR8             CNY                           

9238916  Power supply 40W, BS96             CNY                           

9238748  Coupling, CPC, screw, male, V2          CNY