齊一生物試劑盒組分經(jīng)過(guò)重重優(yōu)化，每個(gè)組分的穩(wěn)定性都完美的經(jīng)過(guò)高溫破壞測(cè)驗(yàn)，程度的避免了長(zhǎng)途運(yùn)輸和保存過(guò)程中抗原抗體的失活.齊一生物試劑盒憑借其完美的標(biāo)曲.嚴(yán)格的質(zhì)控.穩(wěn)定高、易保存，操作簡(jiǎn)便，已廣泛應(yīng)用在全國(guó)免疫學(xué)檢驗(yàn)研究所的各領(lǐng)域中.深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠黑色素細(xì)胞抗體(MCAb)ELISA試劑盒廠家】齊一生物試劑盒憑借其完美的標(biāo)曲、嚴(yán)格的質(zhì)控、較高的穩(wěn)定性，在世界范圍內(nèi)的科研試劑領(lǐng)域中脫穎而出深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！

試驗(yàn)所需自備物品:

1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【大鼠黑色素細(xì)胞抗體(MCAb)ELISA試劑盒廠家】試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

操作注意事項(xiàng)：

1.試劑盒應(yīng)按標(biāo)簽說(shuō)明書儲(chǔ)存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過(guò)后的標(biāo)準(zhǔn)品應(yīng)丟棄，不可保存。

2.實(shí)驗(yàn)中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號(hào)的試劑不要混用.請(qǐng)?jiān)谠噭┖斜Ｙ|(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時(shí)，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標(biāo)板時(shí)應(yīng)充分拍干，不要將吸水紙直接放入酶標(biāo)反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長(zhǎng)時(shí)間打開蓋子。底物B對(duì)光敏感，避免長(zhǎng)時(shí)間暴露于光下。避免用手接觸，有毒。實(shí)驗(yàn)完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時(shí)間一樣。

9.按照說(shuō)明書中標(biāo)明的時(shí)間、加液的量及順序進(jìn)行溫育操作。

【大鼠黑色素細(xì)胞抗體(MCAb)ELISA試劑盒廠家】齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

操作步驟
實(shí)驗(yàn)開始前，請(qǐng)?zhí)崆芭渲煤盟性噭�，試劑或樣品稀釋時(shí)，均需混勻，混勻時(shí)盡量避免起泡。每次檢測(cè)都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過(guò)高時(shí)，用樣品稀釋液進(jìn)行稀釋，以使樣品符合試劑盒的檢測(cè)范圍。
1.         加樣：分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測(cè)樣品孔�？瞻卓准訕悠废♂屢�100μl，余孔分別加標(biāo)準(zhǔn)品或待測(cè)樣品100μl，注意不要有氣泡，加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻，酶標(biāo)板加上蓋或覆膜，37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性，每次實(shí)驗(yàn)請(qǐng)使用新的標(biāo)準(zhǔn)品溶液。
2.         棄去液體，甩干，不用洗滌。每孔加生物素標(biāo)記抗體工作液 100μl（取1μl生物素標(biāo)記抗體加99μl生物素標(biāo)記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時(shí)內(nèi)配制），37℃,60分鐘。
3.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4.         每孔加辣根過(guò)氧化物酶標(biāo)記親和素工作液（同生物素標(biāo)記抗體工作液） 100μl，37℃，60分鐘。
5.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內(nèi)，此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性，底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8.         用酶聯(lián)儀在450nm波長(zhǎng)依序測(cè)量各孔的光密度（OD值）。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測(cè)。
【大鼠黑色素細(xì)胞抗體(MCAb)ELISA試劑盒廠家】注：
1. 用戶在初次使用試劑盒時(shí)，應(yīng)將各種試劑管離心數(shù)分鐘，以便試劑集中到管底。
2. 每次實(shí)驗(yàn)留一孔作為空白調(diào)零孔，該孔不加任何試劑，只是*后加底物溶液及2N H2SO4。測(cè)量時(shí)先用此孔調(diào)OD值至零。
3. 為防止樣品蒸發(fā)，試驗(yàn)時(shí)將反應(yīng)板放于鋪有濕布的密閉盒內(nèi)，酶標(biāo)板加上蓋或覆膜。
4. 未使用完的酶標(biāo)板或者試劑，請(qǐng)于2-8℃保存。標(biāo)準(zhǔn)品、生物素標(biāo)記抗體工作液、辣根過(guò)氧化物酶標(biāo)記親和素工作液請(qǐng)依據(jù)所需的量配置使用。請(qǐng)勿重復(fù)使用已稀釋過(guò)的標(biāo)準(zhǔn)品、生物素標(biāo)記抗體工作液或、辣根過(guò)氧化物酶標(biāo)記親和素工作液。
5. 建議檢測(cè)樣品時(shí)均設(shè)雙孔測(cè)定，以保證檢測(cè)結(jié)果的準(zhǔn)確性。
以上資料僅供您參考，如有問(wèn)題歡迎來(lái)電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9239566 Packaging, LX100 Optics Platform        CNY             【大鼠黑色素細(xì)胞抗體(MCAb)ELISA試劑盒廠家】

9239558 Spring, XYP Torsion Door (Set of 5)     CNY            

9019925 PCB, Mainboard (Microbrd not incl.), Cxx        CNY            

9238867 Premium Cover Agreement MDx, 2PM, 1yr   The Premium Cover Agreement MDx, 2PM provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).   CNY            

9241213 Full Agreement Depot Repair, PtWash, 1yr    "The Full Agreement Depot Repair, PtWash provides full service coverage for the plate washer used in the digene HPV application. The service is conducted as an instrument exchange service.

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."   CNY            

9241199 QIAxcel, Full Agreement, no PM  The Full Depot Repair Agreement, QIAxcel covers all shipping, labor ａnd repair part costs related to an instrument repair for 1 year. Service turn around time is 7-10 working days.   CNY            

9238821 Rect.  carrier for 2 Falcon tubes 50 ml     CNY            

9019469 COMB CASTING 0.2MM X 52 WELLS       CNY            

9243649 Acceler. Valid Support, Other Quant. Sys    The QIAGEN Accelerated Validation Support Other Quantification System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Applications Specialist.  A QIAGEN specialist will provide hands-on user reaction setup, ａnd best practices.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product. Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Plexor HY, Quantifiler Human, Quantifiler Y, Quantifiler Duo)  CNY            

9233373 Spring, gas-driven, cover, BR8000       CNY            

9233339 Valve, throttle     CNY            

9237335 BR MDx, Full Agreement  The Full Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).   CNY            

9237148 XYP Fan     CNY            

9237136 #Twister II, Full Agreement, no PM  The Full Agreement BR Twister II provides full service coverage for the BioRobot Twister II. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Full Agreement, there is a priority response time of 48 hours (2 working days).  CNY            

9237128 Upgrade App., RNeasy 96, BR8000     CNY            

9237119 Upgrade App., MagAttract Miniprep       CNY            

9237105 Protocol Dev./Opt., on-site (1-day) The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 8 hours by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed. CNY             【大鼠黑色素細(xì)胞抗體(MCAb)ELISA試劑盒廠家】

9236419 Buzzer, TWI     CNY            

9236411 Fingers, deepwell, kit, TWI     CNY            

9232967 Board, X-main adapter, BR8000 v1.00     CNY            

9232951 Bar code reader adapter Board, E10084       CNY            

9236360 Syringe Cylinder with Seal  "syringe cylinder ａnd seal for Luminex. We apparently do not sell the seal separate from the cylinder. These items are replaced at annual PM＇s, but customer＇s may want to replace them more often ａnd would be responsible for paying for them on their own then.

Source: Glen"   CNY            

9232920 Card, x-main, PCON, v1.04       CNY            

9232914 PCB, RS232, X-main      CNY            

9236349 375mA Fuse sensor distribution Board        CNY            

9236332 Compressor      CNY            

9236322 Air Solenoid        CNY            

9236162 O-Ring, replacement kit, RapidPlate     CNY            

9236152 Locator Plate, Tips, BR Rapid Plate     CNY            

9236147 Disposal Chute, side, BR Rapid Plate        CNY            

9230778 Spritzschutz Waschstation Plato 7       CNY            

9230776 Scannerdeckel Rose 80       CNY            

QY-07580    MSH2 (mutS homolog 2)MSH2抗原        0.5mg/6501mg/1100             

QY-04530    NKR(Neuromedin B Receptor)神經(jīng)激肽B受體（抗原）         0.5mg/6501mg/1100             

QY-07070    NF-L（Neurofilament triplet L)低分子量神經(jīng)絲蛋白（抗原）         0.5mg/6501mg/1100             

QY-07100    NF-M (Neurofilament triplet M）中分子量神經(jīng)絲蛋白（抗原）        0.5mg/6501mg/1100             

QY-07080    NF-H（Neurofilament triplet H)高分子量神經(jīng)絲蛋白(抗原)       0.5mg/6501mg/1100             

QY-04480    NIS(Na+/I-symporter;NIS)鈉碘轉(zhuǎn)運(yùn)體蛋白（多肽抗原）       0.5mg/6501mg/1100             

QY-04650    NFKBp65(p65 NF-kappa B;p65NFKB)細(xì)胞核因子或稱k基因結(jié)合核因子(多肽抗原)      0.5mg/6501mg/1100             

QY-03230    NR2A （Glutamate receptor2A）谷氨酸受體（N端抗原）       0.5mg/6501mg/1100             

QY-06930    NRP-1（neuropilin-1）神經(jīng)纖毛蛋白-1(抗原)        0.5mg/6501mg/1100             

QY-06940    NRP-2（neuropilin-2）神經(jīng)纖毛蛋白-2（抗原）      0.5mg/6501mg/1100             

QY-07000    NSBP1(Nucleosome-binding protein 1)核小體結(jié)合蛋白1(抗原)         0.5mg/6501mg/1100             

QY-00460    NTP，Neurual thread protein神經(jīng)絲蛋白（抗原）        0.5mg/6501mg/1100              【大鼠黑色素細(xì)胞抗體(MCAb)ELISA試劑盒廠家】

QY-06060    OAT-1 (Organic anion transporter 1)human 陰離子轉(zhuǎn)運(yùn)蛋白-1(人源）         0.5mg/6501mg/1100             

QY-06070    OAT-1 (Organic anion transporter 1)mouse、rat陰離子轉(zhuǎn)運(yùn)蛋白-1（大、小鼠同源：人源見RC-0606P）         0.5mg/6501mg/1100             

QY-06090    OAT-3(Organic anion transporter 3) 陰離子轉(zhuǎn)運(yùn)蛋白-3(抗原)        0.5mg/6501mg/1100             

QY-06040    UAT (Urate transporter/channel)尿酸鹽轉(zhuǎn)運(yùn)子（抗原）      0.5mg/6501mg/1100             

QY-08300    Oct-4胚胎干細(xì)胞關(guān)鍵蛋白(多肽)        0.5mg/6501mg/1100             

QY-00260    OPN(osteopontin)骨橋蛋白（抗原）         0.5mg/6501mg/1100             

QY-07400    P16/CDKN2A (Cyclin-dependent kinase inhibitor-2A)抑癌基因p16（抗原）         0.5mg/6501mg/1100             

QY-07410    p21/WAF1/CIP1 p21 核分裂抑制因子（抗原）         0.5mg/650             

QY-07420    p27 (cyclin-dependent kinase inhibitor 1B)p27(抗原)      0.5mg/6501mg/1100             

QY-07230    P63 proteinP63 腫瘤抑制基因（抗原）      0.5mg/6501mg/1100             

QY-04310    OPG(Osteoprotegerin)護(hù)骨素(多肽抗原)         0.5mg/6501mg/1100             

QY-07470    OPGL (Osteoprotegerin ligand)骨保護(hù)蛋白配體(抗原)        0.5mg/6501mg/1100             

QY-04270    P311 protein增生性瘢痕相關(guān)蛋白（抗原）       0.5mg/6501mg/1100             

QY-07690    PARP(poly ADP-ribose polymerase)多腺苷二磷酸多聚酶抗原       0.5mg/6501mg/1100             

QY-02700    Visfatin-1 (PBEF1)(NT) Human 內(nèi)脂素/內(nèi)臟脂肪素（人：N端多肽）、內(nèi)臟脂肪素、 又稱：前B細(xì)胞克隆增強(qiáng)因子、內(nèi)肥素、腹脂素         0.5mg/6501mg/1100             

QY-02720    Visfatin-1 (PBEF1)(NT) hu、 mo、 rat、MK內(nèi)脂素/內(nèi)臟脂肪素（人、大、小鼠、猴同源：N端多肽）、內(nèi)臟脂肪素、前B細(xì)胞克隆增強(qiáng)因子、內(nèi)肥素、腹脂素         0.5mg/6501mg/1100              【大鼠黑色素細(xì)胞抗體(MCAb)ELISA試劑盒廠家】

QY-02730    Visfatin-2(CT)hu、mo、rat、MK內(nèi)脂素/內(nèi)臟脂肪素（抗人、大、小鼠、猴：N端多肽）前B細(xì)胞克隆增強(qiáng)因子、內(nèi)肥素、腹脂素         0.5mg/6501mg/1100             

QY-07310    PEDF (pigment epithelium-derived factor)色素上皮源性因子(多肽抗原）      0.5mg/6501mg/1100             

QY-07390    PAP2c (Phosphatidic acid phosphatase 2c)磷脂酸磷酸酶2C（抗原）       0.5mg/6501mg/1100