【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【大鼠血管緊張素Ⅰ(Ang-Ⅰ)ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【大鼠血管緊張素Ⅰ(Ang-Ⅰ)ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【大鼠血管緊張素Ⅰ(Ang-Ⅰ)ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【大鼠血管緊張素Ⅰ(Ang-Ⅰ)ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9238685  Mains switch ETA, 10 A              CNY                            【大鼠血管緊張素Ⅰ(Ang-Ⅰ)ELISA試劑盒現(xiàn)貨】

9231656  Holder, CCD Camera BL-185        CNY                           

9231538  Needle, W-Drive, DISU, BR8        CNY                           

9014876  Microplate Station Adapter            CNY                           

9014556  Shaker Adapter 8000  24-tubes  13ml              CNY                           

9014499  Disposable Tip Station, BR8, RD v1.0         CNY                           

9022574  Strut assembly, Hood, SP, right, Qsym        CNY                           

9238348  Upgrade, Precision Pippeting Precision pipetting upgrade includes onsite installation ａnd calibration visit including all travel ａnd labor to install the precision pipetting system, as well as the following components: 4x100ul syringes, 4x 3 Port Valves, 24x96 10ul Disposable Tips ａnd a precision Pipetting Calibration Kit.    CNY                           

9022379  Notebook, RGQ            CNY                           

9021984  Transport lock, QXA            CNY                           

9021892  Bracket, optical sensor, angled, QIAC          CNY                           

9021613  Kit, upgrade QSSP Cabinet, Qsym        CNY                           

9238605  Centrifuge Motor, complete          CNY                           

9238552  RS232 box, online fill ready, RapidPlate              CNY                           

9238253  Syringe Seal           CNY                           

9238222  De-Bubbler Top             CNY                           

9240674  Clamp Kit, Sensor, Reagent Jar, AP96/196           CNY                            【大鼠血管緊張素Ⅰ(Ang-Ⅰ)ELISA試劑盒現(xiàn)貨】

9240665  Nut, Flangeless,1/4-28, White, AP        CNY                           

9240656  Rebuild Kit, Gast Pump, AP96             CNY                           

9240646  External BCR, 2D, HHP, AP          CNY                           

9240619  Fuse, TE5, 2.0A, PCB, AP96/196         CNY                           

9240610  Hall Effects Sensor, Latch Lock, AP98        CNY                           

9240609  Fiber Optic Sensor, Sub-unit, AP98             CNY                           

9240587  Vacuum Switch, Pump, AP96              CNY                           

9240579  Vacuum Switch Assy, Shaker, AP96            CNY                           

9240577  Cable Assy, Capper, X52 CW, AP96/196             CNY                           

9240525  Cable, Motor Axis #2, Elevator, AP98          CNY                           

9240518  Communication Module, RS232-485, AP196              CNY                           

9240502  PM Kit, AP96         CNY                           

9240348  Autopure, Premium Agreement     The Premium Cover Agreement Autopure provides full service coverage for the Autopure LS System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).  CNY                           

9240241  Housing, disposable tip adapter, TR             CNY                           

9239957  EZ1, PM Service    Service covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.   CNY                           

QY-08120       IL-1β(Interleukin-1β)白介素1β（白細(xì)胞介素-1β）(多肽抗原)               0.5mg/6501mg/1100                         

QY-04450       IL1RAPL1 （Interleukin 1 receptor accessory protein-like 1） 白介素受體相關(guān)蛋白樣1前體蛋白（抗原）            0.5mg/6501mg/1100                         

QY-03790       IL-6(Interleukin-6)白介素6（白細(xì)胞介素-6）(多肽抗原)               0.5mg/6501mg/1100                         

QY-06980       IL-10(Interleukin-10)白介素10（白細(xì)胞介素-10）(抗原)               0.5mg/6851mg/1100                         

QY-0698  IL-10(Interleukin-10)白介素10（白細(xì)胞介素-10）(抗原)               0.5mg/6501mg/1100                         

QY-07800       IL-8白介素8（白細(xì)胞介素-8）(抗原)              0.5mg/650                         

QY-06810       Insulin Receptor（ISR）胰島素受體（抗原）           0.5mg/6501mg/1100                         

QY-06410       ITG-α(Integrin α chain)整合素-α（抗原）          0.5ml/6501ml/1100                           

QY-07050       LDL-R(Low-density lipoprotein receptor precursor)低密度脂蛋白受體(抗原)         0.5mg/6501mg/1100                         

QY-07220       Ki-67 AntigenKi-67 Antigen(抗原)         0.5mg/6501mg/1100                         

QY-07490       Kiss-1(Metastasis-suppressor KiSS-1 precursor)腫瘤轉(zhuǎn)移抑制基因（抗原)            0.5mg/6501mg/1100                          【大鼠血管緊張素Ⅰ(Ang-Ⅰ)ELISA試劑盒現(xiàn)貨】

QY-08640       重組K3蛋白          0.5mg/650                         

QY-04090       Leptin瘦素（抗原)               0.5mg/6501mg/1100                         

QY-07590       Lpin1 proteinLpin1 protein(抗原)          0.5mg/6501mg/1100                         

QY-04840       Lumbrokinase蚯蚓纖溶酶(蚓激酶抗原)             0.5mg/6501mg/1100                         

QY-07380       Livin （Inhibitors of apoptosis proterins Livin）一種新的凋亡抑制蛋白(抗原)              0.5mg/6501mg/1100                         

QY-06370       p38 MAPK絲裂原活化蛋白激酶p38(抗原)               0.5mg/6501mg/1100                         

QY-04010       Metal ion transporter擬南介金屬離子轉(zhuǎn)運(yùn)蛋白(抗原)              0.5mg/6501mg/1100                         

QY-06420       MADCAM-1黏膜選址素（抗原）              0.5mg/6501mg/1100                         

QY-07920       Maspin (mammary serine protease inhibitor)抑癌基因(抗原)             0.5ml/6501ml/1100                           

QY-02570       MAG-a/b (Myelin associated glycoprotein; L / S -MAG;)髓鞘相關(guān)糖蛋白a/b(抗原)               0.5mg/6501mg/1100                           

QY-02580       MAG (Myelin associated glycoprotein; L-MAG;MAG-a )髓鞘相關(guān)糖蛋白(抗原)             0.5mg/6501mg/1100                         

QY-02580       MAG-a (Myelin associated glycoprotein; L-MAG; )髓鞘相關(guān)糖蛋白-a(抗原)           0.5mg/650                         

QY-04630       MMP-1(Mous ematrix metalloproteinases-1)基質(zhì)金屬蛋白酶-1（小鼠抗原）         0.5mg/6501mg/1100                         

QY-07610       midnolin isoform Protein 1中腦核仁蛋白1（抗原）         0.5mg/6501mg/1100                         

QY-07620       midnolin isoform Protein 2中腦核仁蛋白2（抗原）         0.5mg/6501mg/1100                         

QY-08320       MICA(MHC class I polypeptide-related sequence A)一種細(xì)胞應(yīng)激分子抗原            0.5mg/6501mg/1100                          【大鼠血管緊張素Ⅰ(Ang-Ⅰ)ELISA試劑盒現(xiàn)貨】

QY-06560       MRP3(Multidrug Resistanec-Associated Protein 3)多藥耐藥相關(guān)蛋白3(抗原)         0.5mg/6501mg/1100                         

QY-06570       MRP1(Multidrug Resistanec-Associated Protein 1)多藥耐藥相關(guān)蛋白1(抗原)         0.5mg/6501mg/1100                         

QY-06610       LRP/MVP (Lung resistance related protein)肺耐藥相關(guān)蛋白(抗原)           0.5mg/6501mg/1100                         

QY-03000       MSLN(mesothelin)間皮素(多肽抗原)          0.5mg/6501mg/1100