價格詳細(xì)資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)產(chǎn)品。

【大鼠β2糖蛋白1抗體IgA/G/M(β2-GP1IgA/G/M)ELISA試劑盒廠家】注意事項

1. 當(dāng)混合蛋白溶液時應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測物質(zhì)含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測溶液工作液時，請以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【大鼠β2糖蛋白1抗體IgA/G/M(β2-GP1IgA/G/M)ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【大鼠β2糖蛋白1抗體IgA/G/M(β2-GP1IgA/G/M)ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【大鼠β2糖蛋白1抗體IgA/G/M(β2-GP1IgA/G/M)ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

336111     Custom ELISArray              CNY                              【大鼠β2糖蛋白1抗體IgA/G/M(β2-GP1IgA/G/M)ELISA試劑盒廠家】

940057     BioSprint 96 DNA Blood Kit (384)              CNY                             

334471     EpiTect ChIP OneDay Kit (12)            CNY                             

331260     miScript PreAMP Custom Primer Mix                CNY                             

331001     RT² miRNA qPCR Assay (200)            CNY                             

9010969  Plate stacker, detachable         Unit for stacking up to ten 96-well plates; for use with the robotic handling system      CNY                             

9010928  Motor, X, SamTrack, P3xxx                CNY                             

979302     PyroMark Q24 Cartridge (3) CE        For delivery of nucleotides ａnd reagents for use in PyroMark Q24. CE-Marked      CNY                             

981303     Rotor-Disc 72 (240)           CNY                             

981005     PCR Tubes, 0.2 ml  (1000)                 CNY                             

9012976  Gripper, left, landoplasted, MTP 120                CNY                             

931955     QIAsymphony Certal Vaccine NA Kit (96)                  CNY                             

929707     QX Cartridge Stand Cover (1)            CNY                             

133252     NeXtalStock Sodium phosphate pH 7 (200)               CNY                             

929606     QX FA Separation Buffer (40 ml)                CNY                             

9010509  Motor, turntable 1/2 MTP, P3/7                  CNY                             

69525       mericon DNA Bacteria Kit (100)                CNY                              【大鼠β2糖蛋白1抗體IgA/G/M(β2-GP1IgA/G/M)ELISA試劑盒廠家】

69106       DNeasy Plant Mini Kit (250)     250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)     CNY                             

211015     QuantiTect Virus Kit (1000)               CNY                             

206674     Type-it CNV SYBR Green PCR +qC Kit (400)               CNY                             

9012763  Liquid Handling Kit Plato 1300                   CNY                             

59568       EpiTect Control DNA (1000)              CNY                             

979014     MOTT 16S Primers For PCR               CNY                             

979011     PyroMark Q96 Vacuum Prep Trough (5)  Re-usable plastic troughs for use in all PyroMark Q96 Vacuum Workstations.         CNY                             

59932       EpiTect MethyLight Assay Hs-ONECUT2(100) "10-fold primer TaqMan probe mix, for real-time MethyLight PCR

For TaqMan probe-based real-time quantification of methylated or unmethylated CpG sites"      CNY                             

59695       EpiTect PCR Control DNA Set (100)           CNY                             

206343     Type-it Mutation Detect PCR Kit (200)    "The Type-it Mutation Detect PCR Kit is especially developed for to fast ａnd reliable detection of mutations such as deletions, insertions,and translocations. It is also highly suited for multiplex PCR-based preamplification of SNPs as preparation for genotyping systems like the

SNaPshot Multiplex Kit from ABI

Features

 Successful & reproducible analysis of multiple mutations

 Optimization-free multiplex PCR assay development

 Specific ａnd sensitive coamplification of all fragments

 Optimized protocol for fast ａnd reliable results"        CNY                             

206341     Type-it Mutation Detect PCR Kit (70)      "The Type-it Mutation Detect PCR Kit is especially developed for to fast ａnd reliable detection of mutations such as deletions, insertions,and translocations. It is also highly suited for multiplex PCR-based preamplification of SNPs as preparation for genotyping systems like the

SNaPshot Multiplex Kit from ABI

Features

 Successful & reproducible analysis of multiple mutations

 Optimization-free multiplex PCR assay development

 Specific ａnd sensitive coamplification of all fragments

 Optimized protocol for fast ａnd reliable results"        CNY                             

9012719  Set, Pins, (6 small, 4 large)                CNY                             

929561     QX Size Marker 50 - 800 bp v2.0 (50 µl)            CNY                             

972804     PyroMark Gold Q96 Reagents (5 x 96)     "For performing pyrosequencing reaction on the ID (5x 96) ａnd Q96 MD (15x96)

ID needs more reagents"         CNY                             

301705     HiPerFect Transfection Reagent (1 ml)             CNY                             

 QY-04076          蛇毒巴曲酶抗體     Anti-Batroxobin                                           

 QY-04077          Bax（小鼠來源抗體）  Anti-Bax （Bcl-2 Assaciated X protein）                                           

 QY-04078          Bax抗體           Anti-Bax（Bcl-2 Assaciated X protein）                                              

 QY-04079          Bcl-2抗體        Anti-Bcl-2(B cell lymphoma/leukemia-2)                                             

 QY-04080          Bcl-xL蛋白抗體       Anti-Bcl-xL                                           

 QY-04081         p53正向細(xì)胞凋亡調(diào)控因子抗體         Anti-BBC3/PUMA(bcl-2 binding component 3)                                             

 QY-04082          抗磷酸化Bcl-xL(pSer62)蛋白抗體          Anti-phospho-Bcl-xL(pSer62)                                       

 QY-04083          丁酰膽堿脂酶抗體(N端)       Anti-BCHE(butyrylcholinesterase)NT                                           

 QY-04084          抗丁酰膽堿脂酶單克隆抗體         Anti-BCHE(mouse Anti-butyrylcholinesterase Monoclonal)                                        

 QY-04085          丁酰膽堿脂酶抗體(C端)        Anti-BCHE(butyrylcholinesterase)CT                                            【大鼠β2糖蛋白1抗體IgA/G/M(β2-GP1IgA/G/M)ELISA試劑盒廠家】

 QY-04086          腦源神經(jīng)營養(yǎng)因子/腦衍化神經(jīng)營養(yǎng)因子   Anti-BDNF (Brain-derived Neurotrophin factor)                                         

 QY-04087          一種抑癌基因抗體         Anti-BECN1(Beclin 1)                                           

 QY-04088          β-酪蛋白(抗體)兔抗山羊、綿羊          Anti-Beta-casein(β-casein)                                        

 QY-04089         TNF家族B細(xì)胞激活因子抗體    Anti-BAFF/CD257(B cell activating factor)                                          

 QY-04090          堿性成纖維細(xì)胞生長因子抗體     Anti-BFGF/FGF-2                                          

 QY-04091          骨保護蛋白/骨鈣素抗體        Anti-BGP(Osteocalcin/Bone Gla-protein)                                            

 QY-04092          BH3結(jié)構(gòu)域凋亡誘導(dǎo)蛋白抗體     Anti-Bid (BH3 interacting domain death agonist)                                       

 QY-04093         橋連整合因子蛋白抗體        Anti-BIN1(Bridging integrator protein-1)                                            

 QY-04094          細(xì)胞死亡調(diào)解子抗體     Anti-Bim(Bcl-2 interacting mediator of cell death)                                              

 QY-04095          細(xì)胞凋亡抑制因子5抗體     Anti-BIRC5/Survivin variant                                         

 QY-04096          BK通道蛋白抗體   Anti-BK channel (stretch-activated Kca channel)                                        

 QY-04097          鈣激活鉀通道蛋白抗體         Anti-BKCa channels（calcium-activated potassium channel）                                           

 QY-04098         骨形態(tài)發(fā)生蛋白-2抗體        Anti-BMP2 (bone morphogenetic protein 2)                                      

 QY-04099          骨形態(tài)發(fā)生蛋白4抗體          Anti-BMP4（bone morphogenetic protein 4）                                          

 QY-04100         骨形態(tài)發(fā)生蛋白-7抗體        Anti-BMP-7(bone morphogenetic protein-7)                                      

 QY-04101          骨成型蛋白受體1A抗體       Anti-BMPR-1A(Bone Morphogenetic protein Receptor-1A)                                        【大鼠β2糖蛋白1抗體IgA/G/M(β2-GP1IgA/G/M)ELISA試劑盒廠家】

 QY-04102          腦鈉素/利鈉肽抗體      Anti-BNP （Brain Natriuretic Peptide ）                                         

 QY-04103         B細(xì)胞特異性轉(zhuǎn)錄因子抗體         Anti-BOb-1(B-cell oct-binding protein 1)                                              

 QY-04104          B Raf抗體        Anti-B Raf                                            

 QY-04105          乳腺癌易感基因1抗體(人)  Anti-BRCA1（Breast cancer susceptbility gene 1）(human)                                     

 QY-04106          乳腺癌易感基因1抗體（小鼠 大鼠）       Anti-BRCA1（Breast cancer susceptbility gene 1）（mouse rat）