1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！【大鼠高敏甲狀腺素(u-T4)ELISA試劑盒現(xiàn)貨】

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【大鼠高敏甲狀腺素(u-T4)ELISA試劑盒現(xiàn)貨】齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【大鼠高敏甲狀腺素(u-T4)ELISA試劑盒現(xiàn)貨】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性.

以上資料僅供參考了解，不供于論文寫(xiě)作

【大鼠高敏甲狀腺素(u-T4)ELISA試劑盒現(xiàn)貨】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

211011  QuantiTect Virus Kit (50)       CNY             【大鼠高敏甲狀腺素(u-T4)ELISA試劑盒現(xiàn)貨】

979017  MOTT 16S Primer 2 For Sequencing        CNY            

59824   EpiTect Fast DNA Bisulfite Kit (50)     CNY            

206246  Type-it Microsatellite PCR Kit (2000)       CNY            

9012742 Syringe pump outlet tubing (4.7 m)      CNY            

9012736 Peristaltic pump outlet tubing (1.0 m)      CNY            

9012725 Syringe pump outlet for Plato       CNY            

979001  PyroMark Q96 Plate (100)    "For sample analysis of DNA template prepared with magnetic beads for ID.

Magnetic bead technology instead of vacuum prep tool is used here"  CNY            

9012706 Base, auto vac, BR8000  This is the robovac base for the BR8000 I. There are 5 contacts.    CNY            

133240  NeXtalStock Sodium hydroxide (200)      CNY            

133233  NeXtalStock Sodium cacodyl. pH 7.5 (100)        CNY            

9002195 RGQ 2plex, PC, PrioPLUS, basic, no PM   "RGQ 2plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"  CNY            

9002039 Rotor-Gene Q MDx 5plex HRM, PrioPLUS    "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

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Regional Repair Center service, 7-10 day turn around time

Loaner available in 2 days*

Annual Inspections (1 PM per yearr)

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59436   EpiTect MethyLight PCR Kit (200)        CNY            

971540  therascreen® UGT1A1 Pyro Kit (24)       CNY            

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51504   QIAamp DNA Stool Mini Kit (50)  For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml) CNY             【大鼠高敏甲狀腺素(u-T4)ELISA試劑盒現(xiàn)貨】

9002013 Rotor-Gene Q MDx 2plex HRM System       CNY            

970032  PyroMark Q24 CpG MGMT (4 x 24)  For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.  CNY            

922920  LiquiChip Filter Microplates (10)       CNY            

292023  mericon Cattle (24)     CNY            

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133193  NeXtalStock di-Potassium phosphate (200)        CNY            

9018555 Cover, front, top, EZ1a     CNY            

9018528 User Manual EZ1 Advanced        CNY            

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QY-18722    Anti-TIMP-3(Tissue Inhibitor of Metalloproteinase-3)金屬蛋白酶組織抑制因子-3抗體                       

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QY-18742    Anti-TLR2 (Toll-like receptor 2)Toll樣受體2抗體                       

QY-18752    Anti-TLR4 (Toll-like receptor 4)Toll樣受體4抗體                       

QY-18762    Anti-TLR5/CD285 (Toll-like receptor 5)Toll樣受體5抗體                     

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QY-18782    Anti-TmR2（GDNFRβ；TRN-R；GFR-alpha-2；NTNR-2）膠質(zhì)細(xì)胞系源性神經(jīng)營(yíng)養(yǎng)因子受體β抗體                        【大鼠高敏甲狀腺素(u-T4)ELISA試劑盒現(xiàn)貨】

QY-18792    anti-Tn-C(tenascin-C)腱糖蛋白-C抗體                    

QY-18802    Anti-TNF-α (Mouse Anti-Human Tumor Necrosis Factor-α Monoclonal Antibody)鼠抗人腫瘤壞死因子-α單克隆抗體                       

QY-18812    Anti-TNF-α(Tumor Necrosis Factor-α)腫瘤壞死因子-α抗體                       

QY-18822    Anti-TNF-β(Tumor Necrosis Factor-β)腫瘤壞死因子-β抗體                       

QY-18832    Anti-TPⅠ （topoisomeraseⅠ）拓普西異構(gòu)酶Ⅰ抗體                    

QY-18842    Anti-DNA TPⅡα (DNA topoisomeraseⅡα)DNA拓普西異構(gòu)酶Ⅱ抗體                       

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QY-18912    Anti-TRAF2 （TNF receptor-associated factor 2）腫瘤壞死因子受體相關(guān)因子2抗體                      

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