檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測(cè)方法：ELISA

檢測(cè)類(lèi)型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價(jià)格及詳細(xì)資料：齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠(chéng)為您服務(wù)！本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量.

【大鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值）,通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度.

試劑盒組成：

【大鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對(duì)照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測(cè)樣品孔.在酶標(biāo)包被板上待測(cè)樣品孔中先加樣品稀釋液40μl,然后再加待測(cè)樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動(dòng)混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）.

11. 測(cè)定：以空白空調(diào)零,450nm波長(zhǎng)依序測(cè)量各孔的吸光度（OD值）. 測(cè)定應(yīng)在加終止液后15分鐘以?xún)?nèi)進(jìn)行.

【大鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定,計(jì)算時(shí)請(qǐng)*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請(qǐng)避光保存.

7． 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號(hào)組分不得混用.

10. 如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn).

【大鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒現(xiàn)貨】計(jì)算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計(jì)算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見(jiàn)應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個(gè)月

9234850  LapLink 2000, full version, English             CNY                              【大鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒現(xiàn)貨】

9240786  Update, Capper Up/Down Motor, AP98           CNY                             

9240771  Assy, Centrifuge Rotor Locator, AP96                CNY                             

6000-5011        RCS Syringe                CNY                             

6000-5006        Disposable Tips                  CNY                             

9241755  Introductory training HC2 (on-site)          CNY                             

9241361  Hinge, for cable reels, EZ1a              CNY                             

9240754  Downtube, Reagent #1, AP96/196           CNY                             

9243310  RGQ, Basic Agreement, no PM        "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: not included

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242800  Fan,230V,AP              CNY                             

9242769  Belt, y-drive, portal, Qsym                CNY                             

9242700  QIAcube, Full Agreement         "Repair service delivery: onsite

Service response time (repair): 48 hours/2 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9242690  QIAensemble Decapper, Premium Agreement        The Premium Cover Agreement DCU provides full service coverage for the QIAensemble Decapper for one year.  Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Premium Agreement, there is a priority response time of 24 hours (1 working day).         CNY                             

9242664  Cooling device, BC, Qsym                   CNY                             

9242096  RGQ, Inspection Agreement            CNY                             

5000-1405        Overlay Digene V2, VTX2                   CNY                             

4518303  artus HCV QS-RGQ Kit (24) RUO                CNY                              【大鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒現(xiàn)貨】

130729     Nextal Tubes CubicPhase II Suite               CNY                             

130725     NeXtal Tubes JCSG Core II Suite                 CNY                             

4510005  artus Enterovirus LC RT-PCR Kit (96) RUO                 CNY                             

130716     NeXtal Tubes PEGs II Suite                 CNY                             

129065     MaXtract High Density (100 x 15 ml)                CNY                            

129056     MaXtract High Density (200 x 2 ml)                   CNY                             

120018     QIAquick Buffer Module 8000          CNY                             

4506265  artus HBV RG PCR Kit (96) CE           CNY                             

80284       AllPrep DNA/RNA Micro Kit (50)               CNY                             

79254       RNase-Free DNase Set (50)      1500 units RNase-free DNase I, RNase-free Buffer RDD, ａnd RNase-free water for 50 RNA minipreps          CNY                             

74904       RNeasy Plant Mini Kit (50)       50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers  CNY                             

19583       24-Well Blocks RB (24)     24-well blocks with 10 ml round-bottom wells, 24 per case    CNY                             

74106       RNeasy Mini Kit (250)      250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers     CNY                             

70022       Oligotex mRNA Mini Kit  For 12 mRNA minipreps: 200 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents ａnd Buffers   CNY                             

19560       Collection Microtubes (racked)        Nonsterile polypropylene tubes (1.2 ml), 960 in racks of 96    CNY                             

QY-12482 Anti-Dysbindin/rhogap92b精神分裂癥易感基因抗體                                               

QY-12492 Anti-E2F1轉(zhuǎn)錄因子E2F-1抗體                                                    

QY-12502 Anti-E2F2轉(zhuǎn)錄因子E2F-2抗體                                                    

QY-12512 Anti-E7 protein (Human papillomavirus type 16)人乳頭瘤病毒16型E7抗體                                                   

QY-12522 Anti-EAAT1/Glast(Excitatory amino acid transporters 1)膠質(zhì)細(xì)胞谷氨酸運(yùn)載蛋白1 抗體                                                      

QY-12532 Anti-EBNA-3 (EpsteinBarr nuclear antigens 3)EB病毒編碼核蛋白-3抗體                                                   

QY-12542 Anti-ECE1（Endothelin Converting Enzyme 1）內(nèi)皮素轉(zhuǎn)化酶1ECE1抗體                                                【大鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒現(xiàn)貨】

QY-12552 Anti-ECG2 (sophagus cancer-related gene-2)食道癌相關(guān)基因抗體                                                    

QY-12562 Anti-ECM1 (extracellular matrix protein 1)細(xì)胞外基質(zhì)蛋白1抗體                                                   

QY-12572 Anti-ED-1 (Ectodermal Dysplasia 1)外胚層發(fā)育不良抗體                                                   

QY-12582 Anti-EGF-R （Epidemal growth factor receptor）表皮生長(zhǎng)因子受體抗體                                                       

QY-12592 Anti-EGFR-ⅤⅢ表皮生長(zhǎng)因子受體-8抗體                                                 

QY-12602 Anti-Egr-1 （Early growth response 1）早期反應(yīng)基因-1/應(yīng)急反應(yīng)生長(zhǎng)基因1抗體                                                      

QY-12612 Anti-Emp1 (epithelial membrane protein 1)表皮膜蛋白1抗體                                                  

QY-12622 anti-EMAb（Endometrium）抗子宮內(nèi)膜抗體                                                   

QY-12632 Anti-Endostatin內(nèi)皮抑素/內(nèi)皮他丁抗體                                                   

QY-12642 Anti-EpCAM（Epithelial cell adhesion molecule）上皮細(xì)胞粘附分子/表皮細(xì)胞粘附分子抗體                                                      

QY-12652 Anti-EPEC-IgG O (Anti-Enteropathogenic E.coli )大腸埃希桿菌O抗體                                                       

QY-12662 Anti-EphA2 R(Ephrin A2 Receptor)酪氨酸蛋白激酶受體A2抗體                                                      

QY-12672 Anti-EphB2 R (Ephrin B2 Receptor)酪氨酸蛋白激酶受體B2抗體                                                     

QY-12682 Anti-ER雌激素受體                                                      

QY-12692 Anti-ERAB內(nèi)質(zhì)網(wǎng)Aβ相關(guān)結(jié)合蛋白抗體                                                  

QY-12702 Anti-ERK2/ERK （MAPK-1/2,MAPK-α ，ERK2，MAPK1，MAPK2, p42MAPK）絲裂原活化蛋白激酶1/2抗體                                                      【大鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)ELISA試劑盒現(xiàn)貨】

QY-12712 Anti-MAPK1/ERK3 (Mitogen-activated protein kinase 3; P44MAPK)絲裂原活化蛋白激酶3抗體                                                     

QY-12722 Anti-ER-α（Estrogen）雌激素受體抗體                                                   

QY-12732 Anti-ER-α（Estrogen）雌激素受體α抗體                                              

QY-12742 Anti-ER-α/β Estrpgen Receptor α/β雌激素受體α/β抗體                                              

QY-12752 Anti-ER-β (Estrogen receptor-β)雌激素受體β 抗體                                                     

QY-12762 Anti-ER-β (Estrogen receptor-β)雌激素受體抗體                                                   

QY-12772 Anti-ESA (Epithelial Specific Antigen)上皮特異性抗原抗體                                                

QY-12782 Anti-ET-1 （Endothelin-1）內(nèi)皮素-1抗體