1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【大鼠CXC趨化因子配體16(CXCL16)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【大鼠CXC趨化因子配體16(CXCL16)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【大鼠CXC趨化因子配體16(CXCL16)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性. 【大鼠CXC趨化因子配體16(CXCL16)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費(fèi)代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9235282  Grease, long term             CNY                              【大鼠CXC趨化因子配體16(CXCL16)ELISA試劑盒現(xiàn)貨】

9022809  Detect Barrel, QXA           CNY                             

9239554  ZLV EX Digital PCBA (1.5.7 Firmware)               CNY                             

9239553  Digital PCBA (1.5.7 Firmware)                   CNY                             

9238657  Main cable, centrifuge              CNY                             

9238630  Jumper, 2-channel             CNY                             

9238620  Computer board Zent3, no Eprom            CNY                             

9242063  Evaporation trap, IQ/OQ, QS           CNY                             

9241890  QIArent&go - Rent out fee EZ1 XL            CNY                             

9241825  Pyro Q96 MDa, Full Agreement       The Full Agreement, PyM provides full service coverage for the PyroMark instrument. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one on-site inspection service, with costs for labor ａnd parts covered. With a Full Agreement, there is a response time of 2 working days.         CNY                             

9241167  Board, LED scan, QX                  CNY                             

9241148  Transport lock, Y axis, M48               CNY                             

9241143  Application AUTOPURE (customized)                CNY                             

9241005  QIArent&go - Rent out fee BR Universal                   CNY                             

9240987  Kit, electronic, FFC-plugs, Qsym                CNY                             

9240980  Sensor, tray, sample tracking, Qsym                  CNY                             

9240945  Sensor, xy, portal, Qsym            CNY                              【大鼠CXC趨化因子配體16(CXCL16)ELISA試劑盒現(xiàn)貨】

9234856  Tubing coupling piece for washer liquid            CNY                             

9234852  Card, ISDN, Fritz PCI                  CNY                             

9240783  Update, Weigh Cell, AP96                  CNY                             

9240774  Assy, Transfer Rack, AP96                  CNY                             

9241632  LED Light System, AP                 CNY                             

9241369  Temperature Controller, EZ1a                   CNY                             

9243645  Guided Valid Support, QIA Extraction Sys         The QIAGEN Other Extraction System Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to QIAGEN extraction chemistry utilized in the process, review the appropriate Handbook ａnd User Manual, as well as hands-on chemistry ａnd instrument operation as required.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Biosprint 96, DNA mericon)         CNY                             

9243542  BR MDx, PM Service        "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9243305  Power Cord, India             CNY                             

9243110  CD-RW, Black, AP196/98           CNY                             

9242712  Validation Support, 2d QIAcube       The QIAGEN QIAcube Guided Validation includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on QIAcube operation.  The Guided Validation service includes experimental design consulation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.)      CNY                             

9019226  Fan - rear 92x92, PyroQ24                 CNY                             

9019220  PCB - Distribution robot, PyroQ96MD               CNY                             

1066843  PG Influenza A RT-PCR Assay (48) LE Z              CNY                             

1066848  NDV Univ. RT-PCR REAGENT LE                 CNY                             

         骨細(xì)胞     5 × 105         1ml                             

         滑膜細(xì)胞         5 × 105         1ml                              【大鼠CXC趨化因子配體16(CXCL16)ELISA試劑盒現(xiàn)貨】

         骨骼肌細(xì)胞     5 × 105         1ml                             

         表皮角化細(xì)胞         5 × 105         1ml                             

         真皮成纖維細(xì)胞     5 × 105         1ml                             

         軟骨細(xì)胞         5 × 105         1ml                             

         破骨細(xì)胞         5 × 105         1ml                             

         皮膚肥大細(xì)胞         5 × 105         1ml                             

         前脂肪細(xì)胞     5 × 105         1ml                             

         成骨細(xì)胞         5 × 105         1ml                             

         腦動脈血管內(nèi)皮細(xì)胞     5 × 105         1ml                             

         腦動脈血管平滑肌細(xì)胞         5 × 105         1ml                             

         腦靜脈血管內(nèi)皮細(xì)胞     5 × 105         1ml                             

         腦靜脈血管平滑肌細(xì)胞         5 × 105         1ml                             

         腦膜細(xì)胞         5 × 105         1ml                             

         神經(jīng)元細(xì)胞     5 ×105  1ml                             

         神經(jīng)膠質(zhì)細(xì)胞         5 × 105         1ml                             

         海馬神經(jīng)應(yīng)細(xì)胞     5 × 105         1ml                              【大鼠CXC趨化因子配體16(CXCL16)ELISA試劑盒現(xiàn)貨】

         腦微血管內(nèi)皮細(xì)胞         5 × 105         1ml                             

         腦成纖維細(xì)胞         5 × 105         1ml                             

         神經(jīng)小膠質(zhì)細(xì)胞     5 × 105         1ml                             

         雪旺氏細(xì)胞     5 × 105         1ml                             

         肺微血管內(nèi)皮細(xì)胞         5 × 105         1ml                             

         肺血管平滑肌細(xì)胞         5 × 105         1ml                             

         Ⅱ型肺泡上皮細(xì)胞         5 × 105         1ml                             

         氣管上皮細(xì)胞         5 × 105         1ml                             

         氣道平滑肌細(xì)胞     5 × 105         1ml                             

         肺成纖維細(xì)胞         5 × 105         1ml                             

         支氣管上皮細(xì)胞     5 × 105         1ml                             

         支氣管成纖維細(xì)胞         5 × 105         1ml                             

         心肌細(xì)胞         5 ×105  1ml