1.購買我公司任意ELISA試劑盒指標享受免費代測，并提供技術(shù)指導服務！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標儀（使用前預熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠Ⅳ型膠原(ColⅣ)ELISA試劑盒廠家】三. 仔細閱讀說明書

1.檢查試劑盒標簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標本制備要規(guī)范，每份標本體積要按2-3個復孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實驗者，注意低溫保存

5.準備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【大鼠Ⅳ型膠原(ColⅣ)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標準品稀釋液或者檢測稀釋液，可能在設計時就含有沉淀物。所有試劑在滴入酶標板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產(chǎn)生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應與我公司或代理商的技術(shù)支持聯(lián)系

【大鼠Ⅳ型膠原(ColⅣ)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標本盡早檢測，對收集后當天就進行檢測的標本，及時儲存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶吮�，請造模取材后，將標本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復凍融。部分標本也可加入適當防腐劑，激素類標本需添加抑肽酶。 

2.試劑盒代測標本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術(shù)人員溝通。

3.液體類標本：標本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標及其它特殊要求。

【大鼠Ⅳ型膠原(ColⅣ)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細內(nèi)容

1、ELISA標準曲線；

2、實驗具體詳細步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結(jié)果及結(jié)果說明）；

4、實驗進行階段，我公司客服人員會及時向您匯報實驗進程。

337832     qBiomarker SYBR Fluor Mastermix (12)           CNY                              【大鼠Ⅳ型膠原(ColⅣ)ELISA試劑盒廠家】

337820     qBiomarker SYBR ROX Mastermix (2)               CNY                             

9018917  Adapter, 72x0.1ml Strip Tubes                   CNY                             

9018897  Rotor-Disc Pipetting Aid           CNY                             

9018708  EZ1 Adv. XL Virus Card v2.0               CNY                             

9018570  Guides, door, L/R, EZ1a            CNY                             

670213     ipsogen BCR-ABL1 mbcr (BCR) Kit (24)             CNY                             

389004     Investigatot IDproof Client Key                 CNY                             

383615     Investigator Argus Y-12QS Kit (100)                   CNY                             

383617     Investigator Argus Y-12QS Kit (400)                   CNY                             

381315     Investigator Nonaplex ESS Kit (100)                  CNY                             

130915     NeXtal DWBlock ProComplex Suite          CNY                             

130911     NeXtal DWBlock MBClass Suite                 CNY                             

4801064  careHBV PCR ASSAY V2             CNY                             

6000-5025        MST Conversion System            CNY                             

L2133       Expand 4 Pipettor Manual (CD-ROM)               CNY                             

9240708  Filter, Vacuum, 40 Micron, AP196/98                CNY                              【大鼠Ⅳ型膠原(ColⅣ)ELISA試劑盒廠家】

9243656  Post-validation Competency Training       """Post-validation Competency Lab Training includes training of the laboratory DNA staff on QIAGEN instrumentation ａnd chemistry, ａnd a review of information of the validation design plan ａnd results of the validation study. Training will be presented both via seminar ａnd hands-on-learning. Attendees will receive a Certificate of Attendance from QIAGEN to provide documentation of training. As required by FBI regulations, the laboratory is then responsible for the competency testing of all DNA analysts to perform lab work validated. The training presentation ａnd curriculum vitae of the trainer may be provided upon request.

Note:

The Post-validation Competency Training is required for all Comprehensive Validation Study plans but can be included at zero cost.

The Post-validation Competency Training is optional for both Guided ａnd Accelerated Validation Study plans ａnd so charges should apply accordingly.

"""   CNY                             

9022641  therascreen KRAS Assay Package CE                 CNY                             

5050-12200A   PC Country Kit, EU            CNY                             

274603     flocktype Mycoplasma Ms Ab (5)              CNY                             

285215     cador B. hyodysenteriae PCR Reagent (96)              CNY                             

273405     pigtype Toxoplasma Ab (20)              CNY                             

273801     pigtype Yersinia Ab (1)               CNY                             

280375     virotype BVDV RT-PCR Kit (96)                  CNY                             

937156     QIAsymphony DSP AXpH DNA Kit (192)            CNY                             

9018702  EZ1 Adv. XL DSP DNA Blood Card              CNY                             

9001907  #QIAgility (w/o PC) PrioPLUS   "QIAgility (w/o PC)  Instrument

Installation of QIAgility instrument & Introductory training

Warranty extension to 3 yrs

On site service, 48 hr response

Preventive Maintenance (1 onsite pipetting head service ａnd inspection per year)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (travel, labor, parts)"     CNY                             

800337     Transformation                  CNY                             

9019616  ZLV Rail, worktable, plate, Cx2                  CNY                             

9019882  #Lid with LED, blue, RG6          CNY                             

9020585  Spring - Gas, Door Support, DML 3000             CNY                             

         腎系膜細胞     5 ×105  1ml                             

         膀胱上皮細胞         5 × 105         1ml                             

         膀胱平滑肌細胞     5 × 105         1ml                             

         腎動脈內(nèi)皮細胞     5 × 105         1ml                             

         腎動脈平滑肌細胞         5 × 105         1ml                             

         腎小管上皮細胞     5 × 105         1ml                             

         骨細胞     5 × 105         1ml                             

         滑膜細胞         5 × 105         1ml                             

         骨骼肌細胞     5 × 105         1ml                             

         表皮角化細胞         5 × 105         1ml                             

         真皮成纖維細胞     5 × 105         1ml                             

         軟骨細胞         5 × 105         1ml                             

         成骨細胞         5 × 105         1ml                             

         破骨細胞         5 × 105         1ml                             

         腦動脈血管內(nèi)皮細胞     5 × 105         1ml                              【大鼠Ⅳ型膠原(ColⅣ)ELISA試劑盒廠家】

         腦靜脈血管平滑肌細胞         5 × 105         1ml                             

         腦靜脈血管內(nèi)皮細胞     5 × 105         1ml                             

         腦動脈血管平滑肌細胞         5 × 105         1ml                             

         腦膜細胞         5 × 105         1ml                             

         神經(jīng)元細胞     5 × 105         1ml                             

         神經(jīng)膠質(zhì)細胞         5 × 105         1ml                             

         腦微血管內(nèi)皮細胞         5 × 105         1ml                             

         眼晶狀體上皮細胞         5 × 105         1ml                             

         視網(wǎng)膜微血管內(nèi)皮細胞         5 × 105         1ml                             

         小梁網(wǎng)細胞     5 × 105         1ml                             

         視網(wǎng)膜色素上皮細胞     5 × 105         1ml                             

         視網(wǎng)膜muller細胞         5 × 105         1ml                             

         虹膜色素上皮細胞         5 × 105         1ml                             

         角膜上皮細胞         5 × 105         1ml                             

         角膜內(nèi)皮細胞         5 × 105         1ml                             

         胰島β細胞     5 × 105         1ml                              【大鼠Ⅳ型膠原(ColⅣ)ELISA試劑盒廠家】