【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買(mǎi)說(shuō)明！

本試劑盒僅供研究使用，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過(guò)程中必須堅(jiān)持全面質(zhì)量控制和全過(guò)程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【大鼠白細(xì)胞介素5（IL-5）Elias試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【大鼠白細(xì)胞介素5（IL-5）Elias試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒(méi)有PBS的話也可以用一下生理鹽水，還是用PBS

【大鼠白細(xì)胞介素5（IL-5）Elias試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過(guò)濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【大鼠白細(xì)胞介素5（IL-5）Elias試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問(wèn)題請(qǐng)來(lái)電，齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

9240523  Cable, Motor, Axis #0, Dispense, AP98        CNY                            【大鼠白細(xì)胞介素5（IL-5）Elias試劑盒現(xiàn)貨】

9240507  Software & Std. Protocols, AP196              CNY                           

9240377  QIAcube, Installation     The Installation, QIAcube facilitates the quick implementation of the QIACube into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the QIAcube instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.    CNY                           

9239571  BR Universal, Basic Agreement     The Basic Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9239570  BR Universal, Premium Agreement       The Premium Cover Agreement BR Universal provides full service coverage for the BioRobot Universal System. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9020032  FOOTSET, RGQ           CNY                           

9019986  PCB, small breakout, 12 core, C12              CNY                           

9019479  UPPER BUFFER LID GS             CNY                           

9238864  BR M48, Premium Agreement       The Premium Cover Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day). CNY                           

9238852  LiquiChip, Basic Agreement   The Basic Cover Agreement LiquiChip provides full service coverage for the LiquiChip workstation. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.       CNY                           

9241224  QIAxcel, Inspection Service  "Repair service delivery not applicable

Service response time (repair) not applicable

Periodic inspection/maintenance: yes, 1/agreement year

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: not included

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: Yes, included"       CNY                           

9241197  Silicone sealant, RTV 108, QX             CNY                           

9241183  Sensor, Y, with cable, QX             CNY                           

9233329  Belt, tooth, X-drive, BR8000         CNY                           

9233299  Toothbelt, X-drive, P3xx1            CNY                           

9233276  Motor, bar-code camera, Y-motor         CNY                           

9233272  LabHand motor (Plato 1***)         CNY                            【大鼠白細(xì)胞介素5（IL-5）Elias試劑盒現(xiàn)貨】

9237711  #BR M48, Installation           CNY                           

9237164  Bezel XYP             CNY                           

9237161  Adapter Vertical Height          CNY                           

9237121  Upgrade App., R.E.A.L. Prep 96          CNY                           

9237112  Upgrade App., QIAamp 96 Swab  Take advantage of our instrumentation ａnd molecular biology expertise to install QIAamp 96 DNA Swab on your BioRobot System.  The onsite protocol development visit includes all travel ａnd labor for the visit to install the standard QIAGEN QIAamp 96 DNA Swab protocol.  Also included is a QIAamp 96 DNA Swab BioRobot 9604 Kit (12-plates) SAP #965842  CNY                           

9237103  Upgrade, BR plus 3 ext. devices    Upgrade BioRobot with three approved integration devices  CNY                           

9236355  Sample Needle, short            CNY                           

9232936  Microplate Reader (UV-340nm)Module        CNY                           

9232915  Washer Board        CNY                           

9236325  Sample Valve          CNY                           

9230821  Rack for reagents,Troughs(for 6 troughs)           CNY                           

4823024  careHIV-1 RT-PCR ASSAY           CNY                           

4861014  careEBV PCR ASSAY I         CNY                           

130823    NeXtal CubicPhase 2w Mo/C 8% µplate (2)        CNY                           

130822    NeXtal CubicPhase 1w Mo/C 8% µplate (10)             CNY                           

NG0169  DNA產(chǎn)物純化試劑盒   50T BR                       

NG0170  PCR純化試劑盒   100T       BR                       

NG0171  膠回收試劑盒       50T BR                       

NG0172  玻璃珠DNA膠回收試劑盒  50T BR                       

NG0173  EZSpin柱式DNA膠回收試劑盒/UNIQ-10柱式DNA膠回收試劑盒/EZSpinColumnDNAGelExtractionKit  50T BR                        【大鼠白細(xì)胞介素5（IL-5）Elias試劑盒現(xiàn)貨】

NG0174  EZSpin柱式PAGE膠DNA回收試劑盒/UNIQ-10柱式PAGE膠DNA回收試劑盒/EZSpinColumnPAGEGelDNAExtractionKit  50T BR                       

NG0175  EZSpin柱式PAGE膠OligoDNA回收試劑盒/UNIQ-10柱式PAGE膠OligoDNA回收試劑盒/EZSpinColumnPAGEOligoGelDNAExtractionKit 50T BR                       

NG0176  EZSpin柱式PCR產(chǎn)物純化試劑盒/UNlQ-10柱式PCR產(chǎn)物純化試劑盒/EZSpinColumnPCRProductPurificationKit       50T BR                       

NG0177  96孔PCR產(chǎn)物純化試劑盒/96wellplatePCRProductPurificationKit   5T   BR                       

NG0178  96孔DNA清潔試劑盒/96wellplateDNACleanupKit    5T   BR                       

NG0179  玻璃珠PCR產(chǎn)物純化試劑盒/GoldenBeadsPCRProductPurificationKit     50T BR                       

NG0180  EZSpin柱式質(zhì)粒小量抽提試劑盒/UNlQ-10柱式質(zhì)粒小量抽提試劑盒/EZSpinColumnPlasmidMini-PrepsKit      50T BR                           

NG0181  EZSpin柱式質(zhì)粒中量抽提試劑盒/UNlQ-200柱式質(zhì)粒中量抽提試劑盒/EZSpinColumnPlasmidMedi-PrepsKit   10T BR                           

NG0182  EZSpin柱式質(zhì)粒大量抽提試劑盒/UNlQ-500柱式質(zhì)粒大量抽提試劑盒/EZSpinColumnPlasmidMaxi-PrepsKit   10T BR                           

NG0183  96孔質(zhì)粒DNA小量抽提純化試劑盒/96wellplatePlasmidMini-PrepsKit   5T   BR                       

NG0184  96孔質(zhì)粒DNA小量制備試劑盒/96wellplatepreparationMini-PrepsKit     5T   BR                       

NG0185  EZSpin柱式無(wú)內(nèi)毒素質(zhì)粒抽提試劑盒/UNlQ-500柱式無(wú)內(nèi)毒素質(zhì)粒抽提試劑盒/EZSpinColumnEndotoxin-freePlasmidPrepsKit    50T BR                       

NG0186  EZSpin柱式一步式裂解質(zhì)�？焖俪樘嵩噭┖�/UNlQ-10柱式一步式裂解質(zhì)�？焖俪樘嵩噭┖�/EZSpinColum-steplysisBacterialPlasmidRapidPrepsKit 50T BR                       

NG0187  EZSpin柱式酵母質(zhì)粒抽提試劑盒/UNlQ-10柱式酵母質(zhì)粒抽提試劑盒/EZSpinColumnYeastPlasmidPrepsKit      50T BR                           

NG0188  EZSpin柱式細(xì)菌基因組DNA抽提試劑盒/UNlQ-10柱式細(xì)菌基因組DNA抽提試劑盒/EZSpinColumnBacterialGenomicDNAIsolationKit       50T BR                       

NG0189  96孔細(xì)菌基因組DNA小量抽提試劑盒/96wellplateBacterialGenomicDNAMini-PrepsKit      5T   BR                        【大鼠白細(xì)胞介素5（IL-5）Elias試劑盒現(xiàn)貨】

NG0190  谷氨酰胺測(cè)試盒    50管/48樣     比色法    建成                    

NG0192  谷氨酸測(cè)試盒       50管/48樣     比色法                        

NG0193  谷氨酰胺、谷氨酸兩種均可測(cè)    50管/24樣     比色法                        

NG0194  丙酮酸激酶PK測(cè)試盒 50管/48樣     比色法                        

NG0195  己糖激酶HK測(cè)試盒     30管/28樣     比色法                        

NG0196  醛縮酶ALD測(cè)試盒      50管/24樣     比色法                        

NG0197  乙酰膽堿轉(zhuǎn)移酶ChAT測(cè)試盒     40管/20樣     比色法                        

NG0198  胃蛋白酶測(cè)試盒    50管/24樣     比色法                        

NG0199  胰蛋白酶測(cè)試盒    50管/48樣     比色法                        

NG0200  丙酮酸測(cè)試盒       50管/48樣     比色法