檢測標本：血清,血漿,尿液,胸腹水,腦脊液,細胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價格及詳細資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認同.大品牌保證,價格公道,傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【大鼠微量蛋白(mALB)Elias試劑盒現(xiàn)貨】實驗原理：

   本試劑盒應用雙抗體夾心法測定標本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標抗體復合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標儀在450nm波長下測定吸光度（OD值）,通過標準曲線計算樣品中該產(chǎn)品濃度.

試劑盒組成：

【大鼠微量蛋白(mALB)Elias試劑盒現(xiàn)貨】標本要求：

1．標本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應盡快進行實驗.若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標準品的稀釋與加樣：在酶標包被板上設標準品孔10孔,在、第二孔中分別加標準品100μl,然后在、第二孔中加標準品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標準品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標準品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標準品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標準品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設空白孔（空白對照孔不加樣品及酶標試劑,其余各步操作相同）、待測樣品孔.在酶標包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復5次,拍干.

6.     加酶：每孔加入酶標試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應（此時藍色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應在加終止液后15分鐘以內(nèi)進行.

【大鼠微量蛋白(mALB)Elias試劑盒現(xiàn)貨】注意事項：

1． 試劑盒從冷藏環(huán)境中取出應在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應裝入密封袋中保存.

2． 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果.

3． 各步加樣均應使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差.一次加樣時間控制在5分鐘內(nèi),如標本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時做標準曲線,做復孔.如標本中待測物質(zhì)含量過高（樣本OD值大于標準品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計算時請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標儀讀數(shù)為準.

8． 所有樣品,洗滌液和各種廢棄物都應按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準.

【大鼠微量蛋白(mALB)Elias試劑盒現(xiàn)貨】計算：

以標準物的濃度為橫坐標,OD值為縱坐標,   

在坐標紙上繪出標準曲線,根據(jù)樣品的OD     

值由標準曲線查出相應的濃度；再乘以稀釋     

倍數(shù)；或用標準物的濃度與OD值計算出標     

準曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實際濃度.

試劑盒性能：

1.樣品線性回歸與預期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

9001293  QIAcube (230V)        Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits, 1-year warranty on parts ａnd labor CNY                              【大鼠微量蛋白(mALB)Elias試劑盒現(xiàn)貨】

4866004  PG FMDV RT-PCR Kit (48) LE Z                   CNY                             

4867004  PG FMDV RT-PCR Kit (48) Z               CNY                             

4870004  PG Influenza A RT-PCR Kit (48) Z               CNY                             

130907     NeXtal DWBlock Anions Suite           CNY                             

4559263  artus C.trachomatisPlus RG PCR Kit(24)CE                CNY                             

4601203  artus Malaria RG PCR Kit (24) RUO          CNY                             

4843024  pg HPV PCR ASSAY            CNY                             

130826     NeXtal CubicPhase 3w Mo/C 8% µplate (10)            CNY                             

130727     NeXtal Tubes JCSG Core IV Suite                CNY                             

130703     NeXtal Tubes Cryos Suite          CNY                             

129916     QIAGEN RNase Inhibitor (500 reactions)          "QIAGEN RNase Inhibitor is a protein of nonhuman origin that binds noncovalently to an inactivates a variety of ribonucleases (e.g., RNase A, B, ａnd C). It does not interfere with RNase T1,T2, H, U1, U2, or CL3. QIAGEN RNase Inhibitor is a polyclonal antibody which is highly active ａnd inhibits more than 90% of RNase activity, whereas HPRI (human placental RNase inhibitor) inhibits only 50%. For this reason, it can be used at lower concentrations compared to other RNase

inhibitors. In addition, QIAGEN RNase Inhibitor is stable over a wide pH ａnd temperature range ａnd at varying dithiothreitol (DTT) concentrations. QIAGEN RNase Inhibitor is supplied at a concentration of 4 units per microliter in 2 mM KH2PO4, 8.0 mM Na2HPO4, 3.0 mM KCl, 150 mM NaCl, pH 7.4, ａnd 50% glycerol."  CNY                             

129115     Nuclease-Free Water (1000 ml)                CNY                             

4504263  artus Parvo B19 RG PCR Kit (24) CE                   CNY                             

4506203  artus HBV RG PCR Kit (24) RUO                CNY                             

4509005  artus WNV LC RT-PCR Kit (96) RUO          CNY                             

80311       AllPrep DNA/RNA 96 Kit (4)              CNY                              【大鼠微量蛋白(mALB)Elias試劑盒現(xiàn)貨】

76554       RNAprotect Animal Blood Tubes 500µl (50)              CNY                             

76163       RNAlater TissueProtect Tubes (20x5ml)  For stabilization of RNA in 20 x 500 mg tissue samples: 20 screw-top tubes containing 5 ml RNAlater RNA Stabilization Reagent each     CNY                             

74182       RNeasy 96 Kit (12)   For 12 x 96 total RNA preps: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, RNase-free Reagents ａnd Buffers       CNY                             

74136       RNeasy Plus Mini Kit (250)                 CNY                             

28004       MinElute PCR Purification Kit (50)   50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)    CNY                             

74124       RNeasy Protect Mini Kit (50)   RNAlater RNA Stabilization Reagent (50 ml), 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers       CNY                             

19588       Elution Microtubes CL (24 x 96)                 CNY                             

19133       QIAGEN Proteinase K (10)        10 ml (>600 mAU/ml, solution)  CNY                             

19076       Buffer ATL         200 ml Tissue Lysis Buffer for 1000 preps         CNY                             

19048       EndoFree Plasmid Buffer Set   Buffers P1, P2, P3, QBT, QC, QN, ER, TE, Endotoxin-free water, RNase A; for 10  plasmid mega- or 5 gigapreps (endotoxin-free)    CNY                             

12961       QIAGEN Plasmid Plus Maxi Sample Kit (5)        QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.      CNY                             

12243       QIAfilter Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi Cartridges      CNY                             

12123       QIAGEN Plasmid Mini Kit (25) 25 QIAGEN-tip 20, Reagents, Buffers      CNY                             

133276     NeXtalStock Spermine (100)             CNY                             

4503065  artus CMV LC PCR Kit (96) CE            CNY                             

QY-x2403 人肝細胞QSG-7701                                                      

QY-x2404 豬腎細胞系PK-15                                                

QY-x2405 豬髖動脈內(nèi)皮細胞PIEC                                                       

QY-x2406 前列腺癌細胞系PC-3                                                  

QY-x2407 大鼠腎上腺嗜鉻細胞瘤細胞(高分化)PC-12                                              

QY-x2408 大鼠腎上腺嗜鉻細胞瘤細胞(未分化)PC-12                                              

QY-x2409 人胰腺癌細胞PANC-1                                                  

QY-x2410 逆轉(zhuǎn)錄病毒包被的NIH3T3細胞PA317                                                      

QY-x2411 小鼠骨髓瘤細胞P3/NSI/1-Ag-4【NS-1】                                                    

QY-x2412 鼠成纖維細胞系 NIH/3T3 詢價 ATCC                                                    

QY-x2413 大鼠腎細胞 NRK 詢價 ATCC                                                     

QY-x2414 人胃癌細胞 NCI-N87 [N87] 詢價 ATCC                                                    

QY-x2415 人Burkitt＇s淋巴瘤細胞 NAMALWA 詢價 ATCC                                                       

QY-x2416 人胚肺細胞 MRC-5 詢價 ATCC                                                

QY-x2417 人胃癌細胞 MGC80-3 詢價 ATCC                                                    

QY-x2418 犬腎細胞系 MDCK 詢價 ATCC                                                  【大鼠微量蛋白(mALB)Elias試劑盒現(xiàn)貨】

QY-x2419 牛腎細胞 MDBK (NBL-1) 詢價 ATCC                                                

QY-x2420 人乳腺癌細胞 MDA-MB-231 詢價 ATCC                                                 

QY-x2421 人乳腺癌細胞 MCF7 詢價 ATCC                                                       

QY-x2422 大鼠成肌細胞 L6 詢價 ATCC                                                    

QY-x2423 小鼠成纖維細胞 L929 詢價 ATCC                                                    

QY-x2424 人T淋巴細胞白血病細胞 Jurkat 詢價 ATCC                                                  

QY-x2425 人結(jié)腸癌細胞 HT-29 詢價 ATCC                                                      

QY-x2426 人纖維肉瘤細胞系 HT-1080 詢價 ATCC                                                  

QY-x2427 人卵巢癌細胞 HO-8910                                                     

QY-x2428 人肝正常細胞 HL-7702 詢價 ATCC                                                  

QY-x2429 人原髓細胞白血病細胞 HL-60 詢價 ATCC                                                      

QY-x2430 腎小管株 HK-2 詢價 ATCC                                                        

QY-x2431 小鼠肝癌細胞 Hepa 1-6 詢價 ATCC                                                

QY-x2432 人肝癌細胞系 Hep-3B 詢價 ATCC                                                   

QY-x2433 人喉表皮癌細胞 HEp-2 詢價 ATCC