齊一生物試劑盒組分經(jīng)過重重優(yōu)化，每個組分的穩(wěn)定性都完美的經(jīng)過高溫破壞測驗，程度的避免了長途運輸和保存過程中抗原抗體的失活.齊一生物試劑盒憑借其完美的標曲.嚴格的質(zhì)控.穩(wěn)定高、易保存，操作簡便，已廣泛應(yīng)用在全國免疫學(xué)檢驗研究所的各領(lǐng)域中.深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【大鼠前列腺素F2a（PGF2a）Elias試劑盒廠家】齊一生物試劑盒憑借其完美的標曲、嚴格的質(zhì)控、較高的穩(wěn)定性，在世界范圍內(nèi)的科研試劑領(lǐng)域中脫穎而出深受研究者們的青睞. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

試驗所需自備物品:

1. 酶標儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【大鼠前列腺素F2a（PGF2a）Elias試劑盒廠家】試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

操作注意事項：

1.試劑盒應(yīng)按標簽說明書儲存，使用前恢復(fù)到室溫30分鐘后方可使用。稀稀過后的標準品應(yīng)丟棄，不可保存。

2.實驗中不用的板條應(yīng)立即放回包裝袋中，密封保存，以免變質(zhì)。

3.不用的其它試劑應(yīng)包裝好或蓋好。不同批號的試劑不要混用.請在試劑盒保質(zhì)前使用。

4.使用一次性的吸頭以免交叉污染，吸取終止液和底物A、B液時，避免使用帶金屬部分的加樣器。

5.使用干凈的塑料容器配置洗滌液。使用前充分混勻試劑盒里的各種成份及樣品。

6.洗滌酶標板時應(yīng)充分拍干，不要將吸水紙直接放入酶標反應(yīng)孔中吸水。

7.底物A應(yīng)揮發(fā)，避免長時間打開蓋子。底物B對光敏感，避免長時間暴露于光下。避免用手接觸，有毒。實驗完成后應(yīng)立即讀取OD值。

8.加入試劑的順序應(yīng)一致，以保證所有反應(yīng)板孔溫育的時間一樣。

9.按照說明書中標明的時間、加液的量及順序進行溫育操作。

【大鼠前列腺素F2a（PGF2a）Elias試劑盒廠家】齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標本前都必須有一個完整的計劃。

操作步驟
實驗開始前，請?zhí)崆芭渲煤盟性噭�，試劑或樣品稀釋時，均需混勻，混勻時盡量避免起泡。每次檢測都應(yīng)該做標準曲線。如樣品濃度過高時，用樣品稀釋液進行稀釋，以使樣品符合試劑盒的檢測范圍。
1.         加樣：分別設(shè)空白孔、標準孔、待測樣品孔�？瞻卓准訕悠废♂屢�100μl，余孔分別加標準品或待測樣品100μl，注意不要有氣泡，加樣將樣品加于酶標板孔底部，盡量不觸及孔壁，輕輕晃動混勻，酶標板加上蓋或覆膜，37℃反應(yīng)120分鐘。
為保證實驗結(jié)果有效性，每次實驗請使用新的標準品溶液。
2.         棄去液體，甩干，不用洗滌。每孔加生物素標記抗體工作液 100μl（取1μl生物素標記抗體加99μl生物素標記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時內(nèi)配制），37℃,60分鐘。
3.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4.         每孔加辣根過氧化物酶標記親和素工作液（同生物素標記抗體工作液） 100μl，37℃，60分鐘。
5.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內(nèi)，此時肉眼可見標準品的前3-4孔有明顯的梯度藍色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應(yīng)（此時藍色立轉(zhuǎn)黃色）。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實驗結(jié)果的準確性，底物反應(yīng)時間到后應(yīng)盡快加入終止液。
8.         用酶聯(lián)儀在450nm波長依序測量各孔的光密度（OD值）。 在加終止液后15分鐘以內(nèi)進行檢測。
【大鼠前列腺素F2a（PGF2a）Elias試劑盒廠家】注：
1. 用戶在初次使用試劑盒時，應(yīng)將各種試劑管離心數(shù)分鐘，以便試劑集中到管底。
2. 每次實驗留一孔作為空白調(diào)零孔，該孔不加任何試劑，只是*后加底物溶液及2N H2SO4。測量時先用此孔調(diào)OD值至零。
3. 為防止樣品蒸發(fā)，試驗時將反應(yīng)板放于鋪有濕布的密閉盒內(nèi)，酶標板加上蓋或覆膜。
4. 未使用完的酶標板或者試劑，請于2-8℃保存。標準品、生物素標記抗體工作液、辣根過氧化物酶標記親和素工作液請依據(jù)所需的量配置使用。請勿重復(fù)使用已稀釋過的標準品、生物素標記抗體工作液或、辣根過氧化物酶標記親和素工作液。
5. 建議檢測樣品時均設(shè)雙孔測定，以保證檢測結(jié)果的準確性。
以上資料僅供您參考，如有問題歡迎來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

764114  PAXgene Bone Marrow RNA Tubes (50)      CNY             【大鼠前列腺素F2a（PGF2a）Elias試劑盒廠家】

679913  ipsogen RT Kit (33)     CNY            

203643  HotStarTaq Plus Master Mix Kit (250)    "For 250 x 20 µl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix, containing 250 units of HotStarTaq Plus DNA Polymerase total, 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-free Water

HotStarTaq Plus Master Mix Kits offer all the benefits of great specificity, sensitivity, ａnd performance as HotStarTaq Plus DNA Polymerase NOW in a ｍaster mix format, which offers even faster results ａnd added convenience."   CNY            

201225  Taq PCR Core Kit (1000) "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."    CNY            

201203  Taq DNA Polymerase (250)    "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2

NEW! Now with 10x CoralLoad PCR Buffer

Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity ａnd minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye ａnd a red dye, that facilitate estimation of DNA migration distance ａnd optimization of agarose gel run time."    CNY            

180922  GeneRead Adapter L Set 1plex (12)       CNY            

180912  GeneRead Adapter I Set 1plex (12)       CNY            

159214  QIAcard FTA Indicator Four Spots (25)       CNY            

159201  QIAcard FTA One Spot (100)      CNY            

9235523 Needle cleaning assembly P/N 4567-01        CNY            

9235300 Plunger tip, syringe 2,5ml      CNY            

9235117 Fuse F4A, v1.00     CNY            

9022818 EZ1 Advanced XL Flip Cap Tube Rack      CNY            

9234889 Vacuum manifold, 96 seal        CNY            

9234862 System liquid container (2l Nalgene)        CNY            

9239555 PCBA, Laser Driver, Unlimited       CNY            

9238664 Hinge       CNY             【大鼠前列腺素F2a（PGF2a）Elias試劑盒廠家】

9238662 Lid with window     CNY            

9238659 Cooling compressor T2168GK, 60Hz        CNY            

9238645 Brake resistor 112 Ohm / 100 W      CNY            

9242061 Pipetting container, IQ/OQ, QS      CNY            

9241827 Pyro Q96 ID, Premium Agreement  "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9241166 Board, A/D, QX      CNY            

9240955 Sensor, z, handler, Qsym        CNY            

9240835 QIAcube, Basic Agreement, no PM The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.   CNY            

9240766 32 OUT Module, D2-32TD1, AP96       CNY            

6000-5018   MST STM Rack & Lid (Blue - 2 pos. cal.)     CNY            

9241777 QIAxtractor, Inspection Service "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY            

9241375 Magnet, door lock, top, EZ1a        CNY            

9241334 Keypad foil, EZ1        CNY            

9240750 Belt, Elevator, AP      CNY            

9243648 Acceler. Valid Supp, Other Extract Sys  The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).    CNY            

QY-x1938    ZR-75-1 乳腺導(dǎo)管癌 SF767 腦瘤                       【大鼠前列腺素F2a（PGF2a）Elias試劑盒廠家】

QY-x1939    A10大鼠主動脈平滑肌                        

QY-x1940    人主動脈平滑肌　CNE-1 高分化鼻咽鱗癌 1640+10%胎牛血清                      

QY-x1941    CNE-2 低分化鼻咽鱗癌 1640+10%胎牛血清                      

QY-x1942    KB 口腔表皮樣癌 1640+10%胎牛血清                       

QY-x1943    Hep-2 喉癌 1640+10%胎牛血清                    

QY-x1944    Ec109 食管癌 1640+10%胎牛血清                      

QY-x1945    KYSE 450 食管癌 1640+10%胎牛血清                       

QY-x1946    KYSE 150 食管鱗癌 1640+10%胎牛血清                     

QY-x1947    EC97076 食管癌 1640+10%胎牛血清                    

QY-x1948    BGC-823 胃腺癌 1640+10%胎牛血清                    

QY-x1949    MGC-803 胃腺癌 1640+10%胎牛血清                    

QY-x1950    SGC7901 胃低分化腺癌 1640+10%胎牛血清(P53基因第六外顯子204位密碼發(fā)生缺失突變)                      

QY-x1951    MKN-45 低分化胃癌 1640+10%胎牛血清                     

QY-x1952    HuTu-80 十二指腸腺癌 MEM+非必需氨基酸+10%胎牛血清                      

QY-x1953    Caco-2 結(jié)腸癌 MEM+非必需氨基酸+10%胎牛血清                     

QY-x1954    COLO 205 結(jié)腸癌 1640+10%胎牛血清                        【大鼠前列腺素F2a（PGF2a）Elias試劑盒廠家】

QY-x1955    COLO320DM 結(jié)腸癌 1640+10%胎牛血清                      

QY-x1956    HCT-8 回盲腸腺癌 1640+10%胎牛血清                      

QY-x1957    HCT-116 低分化結(jié)腸腺癌 1640+10%胎牛血清                    

QY-x1958    HT-29 結(jié)腸腺癌 1640+10%胎牛血清                    

QY-x1959    LS-174T 結(jié)腸腺癌 MEM+非必需氨基酸+10%胎牛血清                      

QY-x1960    SW480 結(jié)腸癌 L15                       

QY-x1961    BEL-7402 肝細胞癌 1640+10%胎牛血清                     

QY-x1962    BEL-7405 肝細胞癌 1640+10%胎牛血清                     

QY-x1963    H7402 肝癌 1640+10%胎牛血清                    

QY-x1964    HCC-9204 肝癌 1640+10%胎牛血清                     

QY-x1965    Hep3B 肝癌 DMEM+非必需氨基酸+10%胎牛血清                       

QY-x1966    Hep G2 肝癌 DMEM+10%胎牛血清                       

QY-x1967    SMMC-7721 肝癌 1640+10%胎牛血清                    

QY-x1968    PANC-1 胰腺導(dǎo)管上皮癌 1640+10%胎牛血清