1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【小鼠己糖激酶(HK)ELISA試劑盒價格】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強(qiáng),重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【小鼠己糖激酶(HK)ELISA試劑盒價格】齊一生物科技?xì)g迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【小鼠己糖激酶(HK)ELISA試劑盒價格】齊一生物供應(yīng)的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性. 【小鼠己糖激酶(HK)ELISA試劑盒價格】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9021255  Tool, thermistor calibration, RGx              CNY                              【小鼠己糖激酶(HK)ELISA試劑盒價格】

9021233  Vacuum Pump (UK plug), PyroQ24VW              CNY                             

130927     NeXtal DWBlock JCSG Core IV Suite                   CNY                             

338176     RT² Optical Cap Strips (2 x 24)          CNY                             

9001514  PyroMark Q24 PyroMark Q24 is a fully integrated system that provides real-time sequence information ａnd is highly suitable for epigenetics research ａnd genetic analysis.The product includes PyroMark Q24 system, software ａnd installation.         CNY                             

9001293  QIAcube (230V)        Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits, 1-year warranty on parts ａnd labor CNY                             

4866004  PG FMDV RT-PCR Kit (48) LE Z                   CNY                             

4867004  PG FMDV RT-PCR Kit (48) Z               CNY                             

4870004  PG Influenza A RT-PCR Kit (48) Z               CNY                             

130907     NeXtal DWBlock Anions Suite           CNY                             

4559263  artus C.trachomatisPlus RG PCR Kit(24)CE                CNY                             

4601203  artus Malaria RG PCR Kit (24) RUO          CNY                             

4843024  pg HPV PCR ASSAY            CNY                             

130826     NeXtal CubicPhase 3w Mo/C 8% µplate (10)            CNY                             

130727     NeXtal Tubes JCSG Core IV Suite                CNY                             

130703     NeXtal Tubes Cryos Suite          CNY                             

129916     QIAGEN RNase Inhibitor (500 reactions)          "QIAGEN RNase Inhibitor is a protein of nonhuman origin that binds noncovalently to an inactivates a variety of ribonucleases (e.g., RNase A, B, ａnd C). It does not interfere with RNase T1,T2, H, U1, U2, or CL3. QIAGEN RNase Inhibitor is a polyclonal antibody which is highly active ａnd inhibits more than 90% of RNase activity, whereas HPRI (human placental RNase inhibitor) inhibits only 50%. For this reason, it can be used at lower concentrations compared to other RNase

inhibitors. In addition, QIAGEN RNase Inhibitor is stable over a wide pH ａnd temperature range ａnd at varying dithiothreitol (DTT) concentrations. QIAGEN RNase Inhibitor is supplied at a concentration of 4 units per microliter in 2 mM KH2PO4, 8.0 mM Na2HPO4, 3.0 mM KCl, 150 mM NaCl, pH 7.4, ａnd 50% glycerol."  CNY                              【小鼠己糖激酶(HK)ELISA試劑盒價格】

129115     Nuclease-Free Water (1000 ml)                CNY                             

4504263  artus Parvo B19 RG PCR Kit (24) CE                   CNY                             

4506203  artus HBV RG PCR Kit (24) RUO                CNY                             

4509005  artus WNV LC RT-PCR Kit (96) RUO          CNY                             

80311       AllPrep DNA/RNA 96 Kit (4)              CNY                             

76554       RNAprotect Animal Blood Tubes 500µl (50)              CNY                             

76163       RNAlater TissueProtect Tubes (20x5ml)  For stabilization of RNA in 20 x 500 mg tissue samples: 20 screw-top tubes containing 5 ml RNAlater RNA Stabilization Reagent each     CNY                             

74182       RNeasy 96 Kit (12)   For 12 x 96 total RNA preps: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, RNase-free Reagents ａnd Buffers       CNY                             

74136       RNeasy Plus Mini Kit (250)                 CNY                             

28004       MinElute PCR Purification Kit (50)   50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)    CNY                             

74124       RNeasy Protect Mini Kit (50)   RNAlater RNA Stabilization Reagent (50 ml), 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers       CNY                             

19588       Elution Microtubes CL (24 x 96)                 CNY                             

19133       QIAGEN Proteinase K (10)        10 ml (>600 mAU/ml, solution)  CNY                             

19076       Buffer ATL         200 ml Tissue Lysis Buffer for 1000 preps         CNY                             

19048       EndoFree Plasmid Buffer Set   Buffers P1, P2, P3, QBT, QC, QN, ER, TE, Endotoxin-free water, RNase A; for 10  plasmid mega- or 5 gigapreps (endotoxin-free)    CNY                             

         └ 支氣管上皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 支氣管成纖維細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 心肌細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 心肌成纖維細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 主動脈內(nèi)皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                          【小鼠己糖激酶(HK)ELISA試劑盒價格】

         └ 主動脈平滑肌細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 大隱靜脈平滑肌細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 冠狀動脈平滑肌細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 食管上皮細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 食管平滑肌細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腸平滑肌細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 腸粘膜上皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腸動脈內(nèi)皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腸靜脈內(nèi)皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 肝實質(zhì)細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 肝動脈內(nèi)皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 肝動脈平滑肌細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 小腸血管內(nèi)皮細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 小腸隱窩上皮細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 肝內(nèi)膽管上皮細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 胃粘膜上皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                          【小鼠己糖激酶(HK)ELISA試劑盒價格】

         └ 肝竇內(nèi)皮細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 腦動脈血管內(nèi)皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腦動脈血管平滑肌細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 腦靜脈血管內(nèi)皮細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腦靜脈血管平滑肌細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 腦膜細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 神經(jīng)元細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 神經(jīng)膠質(zhì)細(xì)胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 海馬神經(jīng)元細(xì)胞總DNA  1μg/1μl（10μg/20ug/50ug）