1.購買我公司任意ELISA試劑盒指標(biāo)享受免費代測，并提供技術(shù)指導(dǎo)服務(wù)！

二、實驗材料與試劑配制：

1. 儀器與材料：酶標(biāo)儀（使用前預(yù)熱30分鐘），微量加液器、吸頭、蒸餾水或去離子水，濾紙。

2. 緩沖液使用：加5ul  的緩沖液于50ul  的樣品中，如果樣品量不夠或者不確定，緩沖液和樣品的混合比例不要小于1:10即可混勻，靜置1小時備用（如果標(biāo)本是血清或者血漿，此步驟忽略）。

3. 洗液的配制：按1:100的比例配制洗液備用。

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【小鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)ELISA試劑盒廠家】三. 仔細閱讀說明書

1.檢查試劑盒標(biāo)簽上的有效期。如果超過有效期，請勿使用。

2. 按說明書確定所有試劑齊全（數(shù)量、體積）

3. 標(biāo)本制備要規(guī)范，每份標(biāo)本體積要按2-3個復(fù)孔以上的量制備（貯存），盡量分裝做備份。

4.短期內(nèi)無法實驗者，注意低溫保存

5.準(zhǔn)備好所有實驗額外所需物品，（比如移液器，試管，清洗器，酶標(biāo)儀）

6. 按說明書將所用試劑平衡至室溫，根據(jù)檢測標(biāo)本數(shù)量確定所需試劑的量

7.檢查試劑盒內(nèi)每種試劑的貯存條件，保證所有試劑均按照試劑盒內(nèi)說明書推薦的條件存儲。 【小鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)ELISA試劑盒廠家】

8.檢查不穩(wěn)定或者變質(zhì)的試劑溶液（比如沉淀或變色）。有些試劑，比如標(biāo)準(zhǔn)品稀釋液或者檢測稀釋液，可能在設(shè)計時就含有沉淀物。所有試劑在滴入酶標(biāo)板之前，必需充分混勻。而由于沉淀物的特性，在加入酶標(biāo)板之前，必需不停攪拌。

9.保證充分的孵育時間和溫度。

10.切勿使用不同生產(chǎn)批號的試劑取代現(xiàn)有試劑或混合現(xiàn)有試劑。

11.在混合或溶解蛋白溶液時，避免泡沫產(chǎn)生。

12.在實驗開始之前，安排好實驗流程。在實驗開始之前，清潔工作臺。

13.若有問題，應(yīng)與我公司或代理商的技術(shù)支持聯(lián)系

【小鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)ELISA試劑盒廠家】四、客戶須知

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1.在收集標(biāo)本前都必須有一個完整的計劃，必須清楚要檢測的成份是否足夠穩(wěn)定。我們提倡新鮮標(biāo)本盡早檢測，對收集后當(dāng)天就進行檢測的標(biāo)本，及時儲存在4℃?zhèn)溆茫缬刑厥庠蛐枰芷谑占瘶?biāo)本，請造模取材后，將標(biāo)本及時分裝后放在-20℃或-70℃條件下保存。因冰室與室溫存在一定溫差，蛋白極易降解，直接影響實驗質(zhì)量，所以避免反復(fù)凍融。部分標(biāo)本也可加入適當(dāng)防腐劑，激素類標(biāo)本需添加抑肽酶。 

2.試劑盒代測標(biāo)本的客戶取材前須向我司銷售人員索要說明書，具體操作注意事項請與我司技術(shù)人員溝通。

3.液體類標(biāo)本：標(biāo)本必須為液體，不含沉淀。包括血清、血漿、尿液、胸腹水、腦脊液、細胞培養(yǎng)上清、組織勻漿等。

4.購買前請?zhí)崆案嬖V我們：您樣本的種屬、樣本數(shù)量、待測指標(biāo)及其它特殊要求。

【小鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)ELISA試劑盒廠家】五、代測數(shù)據(jù)詳細內(nèi)容

1、ELISA標(biāo)準(zhǔn)曲線；

2、實驗具體詳細步驟；

3、完整的實驗報告（試劑耗材，實驗方法，實驗結(jié)果及結(jié)果說明）；

4、實驗進行階段，我公司客服人員會及時向您匯報實驗進程。

9239959  EZ1, Premium Agreement       The Premium Cover Agreement EZ1 provides full service coverage for the BioRobot EZ1. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).         CNY                              【小鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)ELISA試劑盒廠家】

9020028  ZB sensor, 320mm, EZ1xl                   CNY                             

9020018  Upgrade kit, vacuum chamber, CAS-1820                 CNY                             

9019987  PCB, small breakout, 8 core, Cxx               CNY                             

9019927  Cover, tip ejector (UV), Cx8               CNY                             

9019926  PCB, Microboard, Cxx                CNY                             

9019488  Filter, polycap incl. tube/coupling, CX1             CNY                             

9238856  #Basic Cover Agreement MDx, 2PM, 1yr         The Basic Cover Agreement MDx, 2PM provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.         CNY                             

9238839  #Twister II, Basic Agreement, no PM      The Basic Cover Agreement BR Twister II provides full service coverage for the BioRobot Twister II. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Cover Agreement, the response time is 5 working days.     CNY                             

9241178  Fuse, 4A, 250VAC, QX                CNY                             

9238830  BR 3000, Basic Agreement      The Basic Cover Agreement 3000 provides full service coverage for the BioRobot 3000. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Basic Cover Agreement, the response time is 5 working days.     CNY                            

9238813  Rectangular carrier for 20 RIA-tubes                CNY                             

9019471  Lid, Polycarb, C1x              CNY                             

9237327  Upgrade, BR & 1 ext. device. w. sched.  Upgrade BioRobot with one approved integration device using scheduling software         CNY                             

9233197  Pump, vacuum, auto vac, BR8000            CNY                             

9237153  Syringe Pump            CNY                             

9237111  Upgrade App., QIAamp 96 Virus     On-site application Upgrade for standard QIAGEN QIAamp 96 DNA Virus  including kit         CNY                              【小鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)ELISA試劑盒廠家】

9237104  Upgrade, BR plus 4 ext. devices       Upgrade BioRobot with four approved integration devices    CNY                             

9237085  LiquiChip, Installation       The Installation, LiquiChip facilitates the quick implementation of the LiquiChip into the lab routine by minimizing the instrument start-up phase. It covers the hardware ａnd software installation of the LiquiChip Advanced instrument ａnd provides the user with an introductory training on operation ａnd user maintenance. The installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.         CNY                             

9236420  Pins, rack alignment, set (5), TWI             CNY                             

9236412  Fingers, landscape, kit, TWI              CNY                             

9232975  Stirrer Adapter Board (Base Plate) PK80                   CNY                             

9232952  YZ 96 Connector Board 3 (Arm), E10085                   CNY                             

9236361  Alignment tube XYP                   CNY                             

9232938  Camera, buffer track, BR8000                   CNY                             

9236347  250mA Fuse sensor distribution Board              CNY                             

9236331  U-Block              CNY                             

9236142  Disposal Chute, straight, BR Rapid Plate                   CNY                             

9230755  Rechen Stirrer Rose 80             CNY                             

9235701  Cable 5V, RCS1                   CNY                             

9230433  Tubing Block              CNY                             

9235298  Plunger tip, syringe 500µl                  CNY                             

         └ 腎皮層上皮細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腎上皮細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 輸尿管上皮細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腎實質(zhì)細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腎系膜細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 膀胱上皮細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 膀胱平滑肌細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腎動脈內(nèi)皮細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 腎動脈平滑肌細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 腎成纖維細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 膀胱成纖維細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 尿道上皮細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 胎盤羊膜細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 胎盤滋養(yǎng)層細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 胎盤絨毛膜細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                          【小鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)ELISA試劑盒廠家】

         └ 子宮平滑肌細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 子宮成纖維細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 卵巢上皮細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 卵巢成纖維細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 卵巢微血管內(nèi)皮細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 子宮內(nèi)膜上皮細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 淋巴內(nèi)皮細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 淋巴成纖維細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 淋巴血管內(nèi)皮細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 臍帶單核細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 外周血白細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 顱蓋造骨細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 滑膜細胞總DNA       1μg/1μl（10μg/20ug/50ug）                                         

         └ 骨骼肌細胞總DNA  1μg/1μl（10μg/20ug/50ug）                                         

         └ 真皮微血管內(nèi)皮細胞總DNA  1μg/1μl（10μg/20ug/50ug）