價格詳細資料：試劑盒價格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實驗室提供*優(yōu)質(zhì)產(chǎn)品。

【小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISA試劑盒廠家】注意事項

1. 當混合蛋白溶液時應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時間控制在5分鐘內(nèi)，如標本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時做標準曲線，做復(fù)孔。

5. 如標本中待測物質(zhì)含量過高，請先稀釋后再測定，計算時請*后乘以稀釋倍數(shù)。

6. 在配制標準品、檢測溶液工作液時，請以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

5000-1320        Label Front Overlay, SKR1                  CNY                              【小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISA試劑盒廠家】

5000-1312        Platform, SKR1                   CNY                             

5000-1274        Plate Mask, DML2            CNY                             

5000-1253        Iris Control PCB, DML2              CNY                             

5050-1071 V.1 CD RomManual-DML2000 Instrument I, 240V                 CNY                             

5050-00031      CONVERTER USB               CNY                             

5000-1509        Control Heater, HTR1                CNY                             

6000-3121        Plate Washer 1 - Replacement Tubing Kit                 CNY                             

6000-3115        Plate Washer I - Manifold Accessory Kit           CNY                             

5000-1502        Fuse 250V 1A, HTR1                  CNY                             

5000-1419        Breaker Circuit 1Pole, VTX2              CNY                             

5198-1220        hc2 HPV DNA Test(High Risk ａnd Low Risk)               CNY                             

EA10224  EASYartus VZV RG_II                 CNY                             

EA10023  EASYartus HSV-1/2 RG_I            CNY                             

PK-01        Mutation Test Kit (25)               CNY                             

9240744  Belt, Reagent Pump , Long, AP96             CNY                             

9240737  Disinfectant Spray, Conflikt, AP                 CNY                              【小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISA試劑盒廠家】

5000-1413        Knob RB673STKWL Black, VTX2                CNY                             

9240698  Cap, Waste Bottle, Modified, AP              CNY                             

9243655  Addl Unit or Protocol, Comp. Valid Supp Each additional instrument (EZ1, QIAgility, QIAsymphony, QIAcube, Rotor-Gene Q) to be included in the Comprehensive validation service catalog no(s). Scope of work defined from the primary unit utilized in the Comprehensive Validation Support.  Refer to the primary part number for additional information. Quantity of additional units is defined by the number of instruments undergoing a Performance Check study to the primary unit utilized in validation.    CNY                             

1024481  #LCX Vacuum System       Holds 24 QIAamp Maxi columns includes connectors, tubing, waste bottles, vacuum gauge, filters, tray, user manual.   CNY                             

9241221  RCS, Installation       RCS Installation facilitates the quick start up of the RCS into the lab routine. It covers hardware ａnd software installation of the system ａnd provides the user with an introductory training on operation ａnd user maintenance. Installation includes labor ａnd travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.   CNY                             

330025     Microbial DNA qPCR Assays              CNY                             

9022438  USB Ethernet Adaptor, PyroQ96               CNY                             

274202     flocktype IBDV Ab (2)                CNY                             

270805     cattletype MAP Ab (20)            CNY                             

287015     cador M. hyopneumoniae PCR Reagent (96)            CNY                             

285815     cador Leptospira PCR Reagent (96)                   CNY                             

285205     cador B. hyodysenteriae PCR Kit (96)                CNY                             

273001     pigtype Salmonella Ab (1)                  CNY                             

9020579  Jumper, Main Board J5, DML 3000           CNY                             

9020590  PCB Assy, Controller, DML 3000                CNY                             

QTR-1090         雞胚致死孤兒病毒（CELO）抗原         0.5ml        凝膠免疫擴散試驗方法,AGID                                  

QTR-1091         禽腺病毒活體抗原         0.5ml        凝膠免疫擴散試驗方法,AGID                                  

QTR-1092         雞胚致死孤兒病毒（CELO）陽性血清         0.5ml        凝膠免疫擴散試驗方法,AGID                                  

QTR-1093         禽腺病毒陽性血清         0.5ml        凝膠免疫擴散試驗方法,AGID                                  

QTR-1094         禽副粘病毒3型陽性血清     0.5ml        凝膠免疫擴散試驗方法,AGID                                  

QTR-1095         全系列大腸桿菌免疫血清OK        F1     1ml  Slide Agglutination   Tests（禽）    

QTR-1096         全系列大腸桿菌免疫血清OK        F103          1ml  Slide Agglutination   Tests（禽）    

QTR-1097         全系列大腸桿菌免疫血清OK        RCV330    1ml  Slide Agglutination   Tests（禽）    

QTR-1098         BRUCELISA       -        400布氏桿菌病檢測試劑盒 400  間接ELISA，牛                 【小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISA試劑盒廠家】

QTR-1099         BRUCELISA       -160布氏桿菌病診斷試劑盒         160  間接ELISA，牛                         

QTR-1100         BRUCELISA       -160M布氏桿菌病診斷試劑盒     160  間接ELISA，奶樣                    

QTR-1101         COMPELISA      -160布氏桿菌病診斷試劑盒         160  競爭ELISA                         

QTR-1102         COMPELISA      -        400布氏桿菌病診斷試劑盒 400  競爭ELISA                

QTR-1103         布氏桿菌病虎紅平板試劑（標準）     100ml       虎紅平板         Rose          Bengal      Test 

QTR-1104         布氏桿菌病虎紅平板試劑（靈敏）     100ml       虎紅平板         Rose          Bengal      Test 

QTR-1105         布氏桿菌病抗原     100ml       乳環(huán)試驗         Milk Ring Test 

QTR-1106         布氏桿菌病抗原     100ml       血清凝集Hemagglutination  test                    

QTR-1107         布氏桿菌病抗原     100ml       補體結(jié)合試驗         CFT                    

QTR-1108         Brucella   ovis陰性血清 1ml  　                      

QTR-1109         牛皰疹病毒1型FTIC標記抗體    2ml  免疫組織學(xué)     Immunohistology                       

QTR-1110         牛呼吸道合胞病毒FTIC標記抗體         2ml  免疫組織學(xué)     Immunohistology                       

QTR-1111         牛病毒性腹瀉病毒FTIC標記抗體         2ml  免疫組織學(xué)     Immunohistology                       

QTR-1112         牛副流感病毒3型FTIC標記抗體         2ml  免疫組織學(xué)     Immunohistology                       

QTR-1113         牛病毒性腹瀉病毒陽性血清         2ml  血清學(xué)檢測     Serology   test          

QTR-1114         牛病毒性腹瀉病毒1型（BVDV1）單抗      1mg 血清學(xué)檢測     Serology   test           【小鼠β淀粉樣蛋白1-42(Aβ1-42)ELISA試劑盒廠家】

QTR-1115         牛病毒性腹瀉病毒1型（BVDV1）單抗      1mg 血清學(xué)檢測     Serology   test          

QTR-1116         牛病毒性腹瀉病毒1型（BVDV1）單抗      1mg 血清學(xué)檢測     Serology   test          

QTR-1117         牛病毒性腹瀉病毒(BDV)單抗        1mg 血清學(xué)檢測     Serology   test          

QTR-1118         牛病毒性腹瀉病毒(BVDV-2   &      BDV)單抗         1mg 血清學(xué)檢測     Serology  

QTR-1119         牛病毒性腹瀉病毒(所有)單抗       1mg 血清學(xué)檢測     Serology   test