齊一生物銷售：021－6034 8496；181214 53965；173021 04490

1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測測定的精度和提高檢測結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個(gè)試劑盒采用stripwell微孔板。這允許用戶在不同的時(shí)間來分析不同數(shù)量的樣品。

3。什么類型的可重復(fù)性的結(jié)果，得到與分析？

每一個(gè)工具都有一個(gè)包含一個(gè)典型數(shù)據(jù)的圖表的手冊。在運(yùn)營商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時(shí)間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個(gè)用戶都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線。

4。是否有可能儲存的試劑比表示？

在不推薦使用條件下的部件組件的存儲，以保證測試的正確性能。

5。我應(yīng)該如何儲存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長期儲存，建議凍結(jié)他們在- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會給出錯(cuò)誤的結(jié)果。

7。我可以使用一個(gè)不建議在試劑盒插入的樣本類型嗎？

該試劑盒已被驗(yàn)證在試劑盒插入的樣品類型。未經(jīng)檢驗(yàn)的樣品類型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動(dòng)態(tài)范圍內(nèi)的測定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線的范圍內(nèi)的值一般是非線性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測的。

9。我每次都要運(yùn)行一個(gè)空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計(jì)算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測到檢測。當(dāng)排除特定的檢測問題的來源時(shí)，它們也非常有幫助。

10。我能改變我在檢測中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計(jì)為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個(gè)組件包含有特定批號的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對這些特定的地段進(jìn)行質(zhì)量檢驗(yàn)。這是從來沒有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線看起來很好，但我沒有得到一個(gè)信號，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個(gè)矩陣的效果可能是掩蓋檢測。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋，請檢查是否正確進(jìn)行稀釋。過稀釋會導(dǎo)致樣品在標(biāo)準(zhǔn)曲線的范圍內(nèi)下降。

13。你怎么推薦我洗盤子？

如果您使用的是一個(gè)自動(dòng)平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動(dòng)洗衣機(jī)也是一樣的。一個(gè)中繼器或洗瓶也可以使用。用戶應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無塵紙。【小鼠β連環(huán)蛋白/聯(lián)蛋白(β-Cat)ELISA試劑盒價(jià)格】

14。我需要用一個(gè)盤子嗎？

可靠的結(jié)果可以得到無板振動(dòng)，但外徑的一般會低于那些使用平板振動(dòng)器。

15。為什么我要用波長校正450-570nm之間？

對于酶聯(lián)免疫吸附試驗(yàn)，在雙波長讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動(dòng)。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個(gè)給定的分析物的量可能會有所不同，不僅從物種的物種，但也組織和細(xì)胞來源。這個(gè)信息的*佳來源是目前的文獻(xiàn)，很容易通過互聯(lián)網(wǎng)在多個(gè)科學(xué)數(shù)據(jù)庫訪問。

18。我的光密度有點(diǎn)高（或較低），比那些在手冊中，與我的試劑盒來了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【小鼠β連環(huán)蛋白/聯(lián)蛋白(β-Cat)ELISA試劑盒價(jià)格】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【小鼠β連環(huán)蛋白/聯(lián)蛋白(β-Cat)ELISA試劑盒價(jià)格】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【小鼠β連環(huán)蛋白/聯(lián)蛋白(β-Cat)ELISA試劑盒價(jià)格】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

133148     NeXtalStock BICINE pH 7.5 (200)              CNY                              【小鼠β連環(huán)蛋白/聯(lián)蛋白(β-Cat)ELISA試劑盒價(jià)格】

1037870  Buffer TLC, 2x (50/60),G            CNY                             

4692103  RespiFinder RG Panel RUO                CNY                             

1031668  Large 96-Rod Cover (16)  Rod cover for use with BioSprint 96 Deep well Rod         CNY                             

9011958  SamTrak Rack for sixteen 12mm tubes             CNY                             

9243827  RCS, Basic Agreement     "- Service response time RCS: 5 working days

- Inspection/maintenance delivery: yes, included; 1 /agreement year

- Cost coverage for repair parts: yes, included

- Cost coverage for labor: yes, included

- Cost coverage for travel: yes, included

- Replacement system provision for DML: yes, included

- Transportation cost coverage for DML: yes, included

For defect units, after customer calling QIAGEN technical service hotline before 12 am:

RCS:

- QIAGEN shall dispatch a specialist to repair the defect instrument at customer site within 5 working days

DML:

- A special package containing a replacement unit shall be sent to the customer within 5 working days.

- Customer shall send the defect unit to QIAGEN Regional Repair Center following the instructions attached with the packaging.  

Note: for DML owned by customer, QIAGEN Regional Repair Center will send back the repaired original unit to customers within 7-10 working days.

PC, Monitor, Heater, Shaker, Washer, Printer ａnd Votexer:

- A new unit shall be sent to customer within  7-10 working days at no extra cost

- Customer shall send the defect unit to QIAGEN for disposal

- On-site installation of PC ａnd application software is not covered by the agreement

The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP"    CNY                             

37003       #Albumin/IgG Depletion Cartridges(3x1ml)              CNY                             

36311       Strep-Tactin Magnetic Beads (2x1ml)      For micro-scale purification of Strep-tagged proteins: 2 x 1 ml Strep-Tactin-charged magnetic agarose beads (10% suspension)      CNY                             

950183     Tissue Digest Reagent (DXT) (250 ml)               CNY                             

950047     QIAxtractor Virus Plasticware          The Reagent Pack, VX,  (#950407) which is used together with the Virus Plasticware Pack, QIAxtractor provides fully automated viral nucleic acid purification on the QIAxtractor. The combination of reagents ａnd plasticware enables purification of viral nucleic acids from liquid samples, with a throughput of 8-96 samples per run.       CNY                             

218413     Rat miScript Primer Assay Set VX.X          CNY                             

9018297  EZ1 Advanced RNA Card           CNY                             

9018296  EZ1 Advanced DNA Buccal Swab Card              CNY                             

5000-1271        Y Drive Track, DML2                  CNY                             

5000-1265        Mirror, DML2            CNY                             

5000-1261        PMT Socket Only, DML2           CNY                             

5050-1072 V.1 CD Rom Manual - V2 Software Tutorial I                   CNY                              【小鼠β連環(huán)蛋白/聯(lián)蛋白(β-Cat)ELISA試劑盒價(jià)格】

5000-1508        Microplate Heater I - FuseInlet1.6A 250V                CNY                             

6000-5002        MST STM Rack (Black - 1 pos. cal.)           CNY                             

6000-4111DEV2.2   RCS User Application Manual (German)           CNY                             

6000-3111V2.20      RCS User Application Manual           CNY                             

5000-1423        Support Side 099A, VTX2          CNY                             

5050-2007        hc2 Software Protocol Package, CE                   CNY                             

PK-02        Mutation Test Kit (100)             CNY                             

9240738  Decon tube, Water, AP98                  CNY                             

5000-1409        Eccentric Assembly V2, VTX2            CNY                             

9242093  QIAxtractor, Inspection Agreement                  CNY                             

69571       DNeasy mericon 96 QIAcube HT Kit (5)            CNY                             

338132     Microbial DNA-Free Water               CNY                             

330530     Supplemental Microbial qPCR MM (Rox)                   CNY                             

275702     flocktype Salmonella Ab (2)               CNY                             

274602     flocktype Mycoplasma Ms Ab (2)              CNY                             

QY-G270  轉(zhuǎn)化的非洲綠猴腎細(xì)胞         COS-7 SV40     成纖維細(xì)胞     貼壁         RPMI-1640       小牛血清        

QY-G271  狗腎細(xì)胞         MDCK      上皮細(xì)胞         貼壁         RPMI-1640       小牛血清        

QY-G272  豬動(dòng)脈內(nèi)皮細(xì)胞     PIEC         上皮細(xì)胞         貼壁         RPMI-1640       小牛血清        

QY-G273  豬干細(xì)胞         MSCs         上皮細(xì)胞         貼壁         DMEM（低糖）     胎牛血清        

QY-G274  人腎上皮細(xì)胞         HK-2          上皮細(xì)胞         貼壁         DMEM/F12      胎牛血清        

QYC01      鹽酸克倫特羅(瘦肉精）快速檢測卡   20T/盒      各種動(dòng)物肌肉組織、肝臟、尿液、血清、飼料等                                

QYC02      萊克多巴胺快速檢測卡         20T/盒      各種動(dòng)物肌肉組織、肝臟、尿液、血清、飼料等                                

QYC03      沙丁胺醇快速檢測卡     20T/盒      各種動(dòng)物肌肉組織、肝臟、尿液、血清、飼料等                                

QYC04      β-興奮劑快速檢測卡   20T/盒      各種動(dòng)物肌肉組織、肝臟、尿液、血清、飼料等                                

QYC05      三聚氰胺快速檢測卡     20T/盒      水產(chǎn)、牛奶、奶粉                                    

QYC06      孔雀石綠快速檢測卡     20T/盒      水產(chǎn)、飼料等                                     【小鼠β連環(huán)蛋白/聯(lián)蛋白(β-Cat)ELISA試劑盒價(jià)格】

QYC07      呋喃唑酮代謝物快速檢測卡         20T/盒      水產(chǎn)、飼料等                                    

QYC08      呋喃它酮代謝物快速檢測卡         20T/盒      水產(chǎn)、飼料等                                    

QYC09      呋喃妥因代謝物快速檢測卡         20T/盒      水產(chǎn)、飼料等                                    

QYC10      呋喃西林代謝物快速檢測卡         20T/盒      水產(chǎn)、飼料等                                    

QYC11      氯霉素快速檢測卡         20T/盒      水產(chǎn)、蜂蜜、牛奶、奶粉                                

QYC12      磺胺類多殘留快速檢測卡     20T/盒      蜂蜜、牛奶、奶粉                                    

QYC13      阿維菌素、伊維菌素快速檢測卡         20T/盒      蜂蜜、牛奶、奶粉                                    

QYC14      氟喹諾酮類快速檢測卡         20T/盒      蜂蜜、牛奶、奶粉                                    

QYC15      己烯雌酚快速檢測卡     20T/盒      水產(chǎn)、蜂蜜、飼料等                                

QYC16      氟喹諾酮總量檢測卡     20T/盒      水產(chǎn)、蜂蜜、飼料等                                

QYC17      磺胺類多殘留檢測卡     20T/盒      水產(chǎn)、蜂蜜、飼料等                                

QYC18      黃曲霉毒素B1快速檢測卡          20T/盒      谷物、牛奶、奶粉                                    

QYC19      黃曲霉毒素M1快速檢測卡         20T/盒      谷物、牛奶、奶粉                                    

QYC20      青霉素快速檢測試紙卡        20T/盒      谷物、牛奶、奶粉                                    

QYC21      β內(nèi)酰胺類快速檢測試紙卡        20T/盒      谷物、牛奶、奶粉                                    

QYC22      紅霉素快速檢測試紙卡        20T/盒      谷物、牛奶、奶粉                                     【小鼠β連環(huán)蛋白/聯(lián)蛋白(β-Cat)ELISA試劑盒價(jià)格】

QYC23      新霉素快速檢測試紙卡        20T/盒      谷物、牛奶、奶粉                                    

QYC24      頭孢噻呋快速檢測試紙卡   20T/盒      谷物、牛奶、奶粉                                    

QYC201    豬瘟抗體檢測卡     20T/盒      抗血清檢測診斷