【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計算
齊一生物供應(yīng)ELISA試劑盒檢測的目的是為實驗提供準(zhǔn)確可靠的定量分析實驗依據(jù)。為了保證實驗數(shù)據(jù)的可靠性，在實驗過程中必須堅持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個完整的計劃。

【小鼠c-junELISA試劑盒價格】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【小鼠c-junELISA試劑盒價格】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍色）

3.動物組織勻漿（細胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測細胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【小鼠c-junELISA試劑盒價格】樣本離心轉(zhuǎn)速及時間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【小鼠c-junELISA試劑盒價格】以上樣本采集及處理方式供您參考，如有問題請來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9012736  Peristaltic pump outlet tubing (1.0 m)          CNY                            【小鼠c-junELISA試劑盒價格】

9012725  Syringe pump outlet for Plato              CNY                           

979001    PyroMark Q96 Plate (100)    "For sample analysis of DNA template prepared with magnetic beads for ID.

Magnetic bead technology instead of vacuum prep tool is used here"  CNY                           

9012706  Base, auto vac, BR8000 This is the robovac base for the BR8000 I. There are 5 contacts.      CNY                           

133240    NeXtalStock Sodium hydroxide (200)          CNY                           

133233    NeXtalStock Sodium cacodyl. pH 7.5 (100)        CNY                           

9002195  RGQ 2plex, PC, PrioPLUS, basic, no PM    "RGQ 2plex, PC Instrument

Installation of RGQ 2plex & Introductory training

Warranty extension to 3 yrs

Repair Center service, response 7-10 days

No Loaner available

No preventive Maintenance

Priority service provision over non-contract customers

Documented installation & repair report

Unlimited number of repair service

All cost covered (Shipping, labor, parts)"     CNY                           

9002039  Rotor-Gene Q MDx 5plex HRM, PrioPLUS  "Rotor-Gene Instrument

Installation of Rotor-Gene & Introductory training

Warranty extension to 3 yrs

Regional Repair Center service, 7-10 day turn around time

Loaner available in 2 days*

Annual Inspections (1 PM per yearr)

Priority service provision over non-contract customers

Documented installation & PM report

Unlimited number of repair service

All cost covered (shipping, labor, parts)"      CNY                           

59436     EpiTect MethyLight PCR Kit (200)              CNY                           

971540    therascreen® UGT1A1 Pyro Kit (24)           CNY                           

133217    NeXtalStock MOPS pH 7.5 (200)        CNY                           

51504     QIAamp DNA Stool Mini Kit (50)  For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml)      CNY                           

9002013  Rotor-Gene Q MDx 2plex HRM System             CNY                           

970032    PyroMark Q24 CpG MGMT (4 x 24)   For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.       CNY                           

922920    LiquiChip Filter Microplates (10)          CNY                           

292023    mericon Cattle (24)        CNY                           

292013    mericon Pig Kit (24)             CNY                            【小鼠c-junELISA試劑盒價格】

9001903  QIAgility HEPA/UV (PC) Priority  QIAgility HEPA/UV (PC) Priority includes the QIAgility instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One on-site pipetting head service is included for each year of the warranty; 2 pipetting head services in total. Service package includes all parts, labor ａnd travel costs with an on site service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                           

133122    NeXtalStock Potassium citrate (200)           CNY                           

963141    QIAquick 96 PCR BioRobot Kit (4)      For purification of 4 x 96 PCR products: 4 QIAquick 96 Plates, Reagents, Buffers, Collection Microtubes (1.2 ml) ａnd Caps, 96-Well Microplates RB ａnd Lids, Tape Pads    CNY                           

133193    NeXtalStock di-Potassium phosphate (200)         CNY                           

9018555  Cover, front, top, EZ1a         CNY                           

9018528  User Manual EZ1 Advanced          CNY                           

37601     Qproteome Plasma Membrane Protein Kit           CNY                           

9015329  O-rings, Norpren tube fixing, M48 (2)         CNY                           

9015328  Seal, cap, M48 (6)         CNY                           

239095    GelPilot 1 kb Plus Ladder (100)    Ready-to-use DNA marker, 600µl (100lanes), 12 fragments: 100 – 10000 bp    CNY                           

133127    NeXtalStock Sodium citrate (200)        CNY                           

133126    NeXtalStock TRIS-HCl pH 8.5 (200)           CNY                           

133008    NeXtalStock 2-Propanol (200)             CNY                           

133007    NeXtalStock MES sod. salt untit. (200)              CNY                           

9011949  Board, X-main              CNY                           

EK0445   mMCSF-ELISA     小鼠巨噬細胞集落刺激因子ELISA試劑盒       Mouse M-CSF ELISA Kit      96 tests                

EK0460   mMMP-2-ELISA    小鼠MMP-2ELISA試劑盒   Mouse MMP-2 ELISA Kit     96 tests                

EK0462   mMMP3-ELISA     小鼠MMP-3ELISA試劑盒   Mouse MMP-3 ELISA Kit     96 tests                

EK0466   mMMP-9-ELISA    小鼠MMP-9ELISA試劑盒   Mouse MMP-9 ELISA Kit     96 tests                

EK0470   mNGF-ELISA 小鼠NGF/NGFβELISA試劑盒  Mouse NGF/NGFβ ELISA Kit    96 tests                

EK0473   mNT3-ELISA 小鼠Neurotrophin-3 ELISA試劑盒     Mouse Neurotrophin-3 ELISA Kit  96 tests                

EK0483   mOPN-ELISA 小鼠骨橋素Elisa試劑盒ELISA試劑盒      Mouse OPN ELISA Kit  96 tests                 【小鼠c-junELISA試劑盒價格】

EK0486   mPDGF-AB-ELISA       小鼠血小板衍生生長因子ABELISA試劑盒      Mouse PDGF-AB ELISA Kit  96 tests                

EK0495   mRantes-ELISA     小鼠趨化因子ELISA試劑盒       Mouse Rantes ELISA Kit       96 tests                

EK0504   mSELL-ELISA       小鼠L選擇素Elisa試劑盒   Mouse L-Selectin ELISA Kit  96 tests                

EK0506   mSELP-ELISA       小鼠P選擇素Elisa試劑盒   Mouse P-Selectin ELISA Kit  96 tests                

EK0515   mTGF     小鼠轉(zhuǎn)化生長因子β1ELISA試劑盒  Mouse TGFβ1 ELISA Kit    96 tests                

EK0527   mTNF-ELISA 小鼠TNFαELISA試劑盒   Mouse TNFα ELISA Kit     96 tests                

EK0538   mVCAM-ELISA     小鼠血管細胞粘附分子-1ELISA試劑盒    Mouse VCAM-1 ELISA Kit    96 tests                

EK0541   mVEGF-ELISA      小鼠血管內(nèi)皮生長因子ELISA試劑盒       Mouse VEGF ELISA Kit 96 tests                

EK0562   mECad-ELISA       小鼠鈣粘著蛋白ELISA試劑盒   Mouse E-Cadherin ELISA Kit 96 tests                

EK0577   mGp130-ELISA     小鼠Gp130/IL6STELISA試劑盒 Mouse Gp130/IL6ST ELISA Kit    96 tests                

EK0582   mRes-ELISA  小鼠抵抗素ELISA試劑盒   Mouse Resistin ELISA Kit     96 tests                

EK0664   mBAFF-ELISA      小鼠BAFFELISA試劑盒     Mouse BAFF ELISA Kit 96 tests                

EK0668   mPCad-ELISA       小鼠P-CadherinELISA試劑盒     Mouse P-Cadherin ELISA Kit 96 tests                

EK0703   mCD40-ELISA      小鼠CD40/TNFRSF5ELISA試劑盒    Mouse CD40/TNFRSF5 ELISA Kit      96 tests                

EK0708   mCD80-ELISA      小鼠B7-1/CD80ELISA試劑盒     Mouse B7-1/CD80 ELISA Kit       96 tests                

EK0717   mCTLA4-ELISA    小鼠CTLA4ELISA試劑盒   Mouse CTLA4 ELISA Kit      96 tests                 【小鼠c-junELISA試劑盒價格】

EK0780   mIL7-ELISA   小鼠IL-7 ELISA試劑盒      Mouse IL-7 ELISA Kit   96 tests                

EK0808   mCSF1R-ELISA    小鼠CSF1R/M-CSFRELISA試劑盒    Mouse CSF1R/M-CSFR ELISA Kit      96 tests                

EK0830   mRANK-ELISA     小鼠Receptor Activator of NF-κB (RANK)ELISA試劑盒       Mouse Receptor Activator of NF-κB (RANK) ELISA Kit 96 tests                

EK0866   mIL23-ELISA 小鼠IL-23ELISA試劑盒      Mouse IL-23 ELISA Kit 96 tests                

EK0874   mPECAM-ELISA   小鼠 PECAM-1/CD31ELISA試劑盒   Mouse PECAM-1/CD31 ELISA Kit       96 tests                

EK0925   mDKK-1-ELISA     小鼠DKK1ELISA試劑盒     Mouse DKK1 ELISA Kit 96 tests                

EK0308   rBDNF-ELISA       大鼠腦源性神經(jīng)生長因子ELISA試劑盒   Rat BDNF ELISA Kit     96 tests