價(jià)格詳細(xì)資料：試劑盒價(jià)格電議，齊一生物銷售：021－6034 8496；181214 53965；173021 04490,齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大研究所.中科院科研單位一致肯定和認(rèn)同。齊一生物大品牌保證，傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)產(chǎn)品。

【小鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒廠家】注意事項(xiàng)

1. 當(dāng)混合蛋白溶液時(shí)應(yīng)盡量輕緩，避免起泡。

2. 洗滌過程非常重要，不充分的洗滌易造成假陽性。

3. 一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4. 請每次測定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。

5. 如標(biāo)本中待測物質(zhì)含量過高，請先稀釋后再測定，計(jì)算時(shí)請*后乘以稀釋倍數(shù)。

6. 在配制標(biāo)準(zhǔn)品、檢測溶液工作液時(shí)，請以相應(yīng)的稀釋液配制，不能混淆。

7. 底物請避光保存。

8. 不要用其它生產(chǎn)廠家的試劑替換試劑盒中的試劑。

FOR RESEARCH USE ONLY

Drug Names

Generic Name：

This kit can be used for determination of serum, plasma ａnd liquid samples Organization Content.

The experimental principle:

The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.

Materials provided with the kit

Materials provided with the kit 96 determinations Storage

User manual   1  

Closure plate membrane   2  

Sealed bags   1  

Microelisa stripplate    1   2-8℃

Standard：360ng/L 0.5ml×1 bottle   2-8℃

Standard diluent  1.5ml×1 bottle   2-8℃

HRP-Conjugate reagent    6ml×1 bottle 2-8℃

Sample diluent    6ml×1 bottle 2-8℃

Chromogen Solution A 6ml×1 bottle 2-8℃

Chromogen Solution B 6ml×1 bottle 2-8℃

Stop Solution 6ml×1 bottle 2-8℃

wash  solution    （20ml×30 fold）

×1bottle  2-8℃

Specimen requirements

1.    serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. 【小鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒廠家】

2.    plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

3.    Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax ａnd cerebrospinal fluid Reference to it.

4.    cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells ａnd release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

5.    Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

6.    extract as soon as possible after Specimen collection,and according to the relevant literature, ａnd should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.

7.    Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.

Assay procedure

1.Dilute ａnd add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first ａnd the second well, then add Standard dilution 50μl to the first ａnd the second well, mix; take out 100μl form the first ａnd the second well then add it to the third ａnd the forth well separately. then add Standard dilution 50μl to the third ａnd the forth well ,mix ; then take out 50μl from the third ａnd the forth well discard, add 50μl to the fifth ａnd the sixth well ,then add Standard dilution 50μl to the fifth ａnd the sixth well, mix ; take out 50μl from the fifth ａnd the sixth well ａnd add to the seventh ａnd the eighth well, then add Standard dilution 50μl to the seventh ａnd the eighth well ,mix ; take out 50μl from the seventh ａnd the eighth well ａnd add to the ninth ａnd the tenth well, add Standard dilution 50μl to the ninth ａnd the tenth well, mix , take out 50μl from the ninth ａnd the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)

2.add sample：Set blank wells separately (blank comparison wells .don’t add sample ａnd HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, ａnd Gently mix. 【小鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒廠家】

3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.

4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water ａnd reserve.

5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well.

7.incubate：Operation with 3.

8.washing：Operation with 5.

9.color：Add Chromogen Solution A 50ul ａnd Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃

10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution ａnd within 15min.

Important notes

1.    The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.    washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.    add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley . 【小鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒廠家】

4.    if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.    Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.    The substrate evade the light preservation.

7.    Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.    All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.    Do not mix reagents with those from other lots.

Calculate：

Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density ａnd the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

Storage ａnd validity

1．Storage：  2-8℃.

2．validity： six months.

9001293  QIAcube (230V)        Robotic workstation for automated purification of DNA, RNA, or proteins using QIAGEN spin-column kits, 1-year warranty on parts ａnd labor CNY                              【小鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒廠家】

4866004  PG FMDV RT-PCR Kit (48) LE Z                   CNY                             

4867004  PG FMDV RT-PCR Kit (48) Z               CNY                             

4870004  PG Influenza A RT-PCR Kit (48) Z               CNY                             

130907     NeXtal DWBlock Anions Suite           CNY                             

4559263  artus C.trachomatisPlus RG PCR Kit(24)CE                CNY                             

4601203  artus Malaria RG PCR Kit (24) RUO          CNY                             

4843024  pg HPV PCR ASSAY            CNY                             

130826     NeXtal CubicPhase 3w Mo/C 8% µplate (10)            CNY                             

130727     NeXtal Tubes JCSG Core IV Suite                CNY                             

130703     NeXtal Tubes Cryos Suite          CNY                             

129916     QIAGEN RNase Inhibitor (500 reactions)          "QIAGEN RNase Inhibitor is a protein of nonhuman origin that binds noncovalently to an inactivates a variety of ribonucleases (e.g., RNase A, B, ａnd C). It does not interfere with RNase T1,T2, H, U1, U2, or CL3. QIAGEN RNase Inhibitor is a polyclonal antibody which is highly active ａnd inhibits more than 90% of RNase activity, whereas HPRI (human placental RNase inhibitor) inhibits only 50%. For this reason, it can be used at lower concentrations compared to other RNase

inhibitors. In addition, QIAGEN RNase Inhibitor is stable over a wide pH ａnd temperature range ａnd at varying dithiothreitol (DTT) concentrations. QIAGEN RNase Inhibitor is supplied at a concentration of 4 units per microliter in 2 mM KH2PO4, 8.0 mM Na2HPO4, 3.0 mM KCl, 150 mM NaCl, pH 7.4, ａnd 50% glycerol."  CNY                             

129115     Nuclease-Free Water (1000 ml)                CNY                             

4504263  artus Parvo B19 RG PCR Kit (24) CE                   CNY                             

4506203  artus HBV RG PCR Kit (24) RUO                CNY                             

4509005  artus WNV LC RT-PCR Kit (96) RUO          CNY                             

80311       AllPrep DNA/RNA 96 Kit (4)              CNY                              【小鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒廠家】

76554       RNAprotect Animal Blood Tubes 500µl (50)              CNY                             

76163       RNAlater TissueProtect Tubes (20x5ml)  For stabilization of RNA in 20 x 500 mg tissue samples: 20 screw-top tubes containing 5 ml RNAlater RNA Stabilization Reagent each     CNY                             

74182       RNeasy 96 Kit (12)   For 12 x 96 total RNA preps: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, RNase-free Reagents ａnd Buffers       CNY                             

74136       RNeasy Plus Mini Kit (250)                 CNY                             

28004       MinElute PCR Purification Kit (50)   50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)    CNY                             

74124       RNeasy Protect Mini Kit (50)   RNAlater RNA Stabilization Reagent (50 ml), 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers       CNY                             

19588       Elution Microtubes CL (24 x 96)                 CNY                             

19133       QIAGEN Proteinase K (10)        10 ml (>600 mAU/ml, solution)  CNY                             

19076       Buffer ATL         200 ml Tissue Lysis Buffer for 1000 preps         CNY                             

19048       EndoFree Plasmid Buffer Set   Buffers P1, P2, P3, QBT, QC, QN, ER, TE, Endotoxin-free water, RNase A; for 10  plasmid mega- or 5 gigapreps (endotoxin-free)    CNY                             

12961       QIAGEN Plasmid Plus Maxi Sample Kit (5)        QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.      CNY                            

12243       QIAfilter Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi Cartridges      CNY                             

12123       QIAGEN Plasmid Mini Kit (25) 25 QIAGEN-tip 20, Reagents, Buffers      CNY                             

133276     NeXtalStock Spermine (100)             CNY                             

4503065  artus CMV LC PCR Kit (96) CE            CNY                             

QY-x2314 HGC-27人胃癌細(xì)胞（未分化）MEM 10%FBS ： 未分化胃癌，能分泌粘液素。                                                      

QY-x2315 Hepa 1-6小鼠肝癌細(xì)胞 高糖DMEM 10%FBS ： 此細(xì)胞株源自C57/L小鼠中引發(fā)的BW7756肝癌。此細(xì)胞可以在無血清的培養(yǎng)基中繁殖，培養(yǎng)基... 人肝癌細(xì)胞系 Hep-3B 詢價(jià) ATCC                                                  

QY-x2316 Hep-3B人肝癌細(xì)胞系 /8EMEM 10%FBS ： 這株細(xì)胞整合了完整的乙型肝炎病毒基因組。                                                   

QY-x2317 HEp-2 人喉表皮癌細(xì)胞HeLa細(xì)胞污染DMEM 10%FBS ： *初認(rèn)為這個(gè)細(xì)胞源自喉上皮癌，但隨后通過同功酶分析、HeLa標(biāo)記染色體和DNA指紋分析...                                                

QY-x2318 Hep G2人肝癌細(xì)胞EMEM 10%FBS ： 該細(xì)胞系來自15歲 性白人的組織。 形態(tài)為上皮形，模式染色體數(shù)為55，在免疫抑制小鼠中不致瘤。                                                      

QY-x2319 Hep 3B2.1-7 人肝癌細(xì)胞EMEM 10%FBS ： 該細(xì)胞系來自8歲 性黑人的組織。 其染色體模式數(shù)目為60，在裸鼠中能致瘤。 已知該細(xì)胞系...                                               

QY-x2320 Hela人宮頸癌細(xì)胞 /31 DMEM 10%FBS ： 是個(gè)來自 和連續(xù)培養(yǎng)維持非整倍體上皮細(xì)胞系。它由G.D.Gey等在1951年從31歲 ... 人子宮內(nèi)膜腺癌細(xì)胞 HEC-1-B 詢價(jià) ATCC                                                    

QY-x2321 HEC-1-B人子宮內(nèi)膜腺癌細(xì)胞 EMEM 10%FBS ： 該細(xì)胞是H. Kuramoto 1968年分離的HEC-1-A細(xì)胞亞株。不同於HEC-A...                                                 

QY-x2322 HEC-1-A 人子宮內(nèi)膜腺癌細(xì)胞McCoy's 5a 10%FBS ： 這株細(xì)胞及其亞株HEC-1-B是H. Kuramot... 人盲腸腺癌細(xì)胞 HCT-8 詢價(jià) ATCC                                                       【小鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒廠家】

QY-x2323 HCT-8人盲腸腺癌細(xì)胞結(jié)腸；回盲腸結(jié)直腸腺癌形態(tài)：RPMI-1640 10%FBS ： HCT-8與HRT-18是一的。 角蛋白免疫過氧化物酶染色陽性。 人結(jié)腸癌細(xì)胞 HCT 116 詢價(jià) ATCC                                                    

QY-x2324 HCT 116 人結(jié)腸癌細(xì)胞 - ： - 人膽管細(xì)胞型肝癌細(xì)胞 HCCC-9810 詢價(jià) ATCC                                                      

QY-x2325 HCCC-9810人膽管細(xì)胞型肝癌細(xì)胞RPMI-1640 10%FBS ： 細(xì)胞株源自一位 性肝膽管細(xì)胞癌患者。                                                    

QY-x2326 HBZY－1 大鼠腎小球系膜細(xì)胞                                                       

QY-x2327 HBL-100 人乳腺細(xì)胞 整合SV40基因的乳腺形態(tài)： DMEM 10%FBS ： HBL-100是一株，E.V. Gaffney及其同事從一位沒有乳...                                                  

QY-x2328 H9c2(2-1)大鼠心肌細(xì)胞心肌層形態(tài)： 成肌細(xì)胞高糖DMEM 10%FBS ： B. Kimes ａnd B. Brandt從源于BD1X大鼠胚胎心臟組織的克隆細(xì)胞株亞克...                                                      

QY-x2329 H9人T淋巴細(xì)胞系T淋巴細(xì)胞形態(tài)： 淋巴細(xì)胞RPMI-1640 10%FBS。 ： The cells produce HTLV III                                                       

QY-x2330 F81貓腎細(xì)胞貓組織 腎形態(tài)： -RPMI 1640+10%胎牛血清或新生牛血清。 ： -                                                    

QY-x2331 EL-4小鼠淋巴瘤細(xì)胞小鼠組織 T 淋巴細(xì)胞；淋巴瘤形態(tài)： 淋巴母細(xì)胞DMEM+10%胎牛血清或新生牛血清。 ： EL4是從用9,10-二甲基-1,2-苯并蒽在C57BL小鼠中誘導(dǎo)...                                                 

QY-x2332 DU 145人前列腺癌細(xì)胞前列腺；轉(zhuǎn)移灶；腦癌形態(tài)： Ham's F12 + 10% 胎牛血清。 ： DU 145 是從一位有三年淋巴細(xì)胞白血病史的前列腺癌患...                                                  

QY-x2333 Daudi 人成巨核細(xì)胞白血病細(xì)胞 淋巴瘤形態(tài)： 淋巴母細(xì)胞含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 10mM HEPES...                                                       

QY-x2334 CRFK貓腎細(xì)胞含 1.5g/L 碳酸氫鈉，0.1mM 非必需氨基酸，1.0mM 丙酮酸鈉，2mM L-谷氨酰胺和Earle's BSS的...                                                      

QY-x2335 COS-7非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞SV40轉(zhuǎn)染形態(tài)： 成纖維細(xì)胞含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 4mM L-谷氨酰胺的DMEM，90%；胎牛血...                                                   

QY-x2336 COLO 320 人結(jié)腸腺癌細(xì)胞RPMI1640+10%胎牛血清�；�DMEM + 10% 新生牛血清。 ： 能合成復(fù)合胺，腎上腺素。                                                        

QY-x2337 CNE 人鼻咽癌細(xì)胞 RPMI 1640+10%胎牛血清或新生牛血清。 ： 此細(xì)胞株來源于一位58歲 性鼻咽癌患者。                                                  

QY-x2338 Chang liver 人張氏肝細(xì)胞 EMEM+10%胎牛血清或RPMI 1640 + 10% 新生牛血清。 ： 張氏肝細(xì)胞1954年從非惡性的人體組織中建立。.                                                  【小鼠轉(zhuǎn)化生長因子β1(TGF-β1)ELISA試劑盒廠家】

QY-x2339 CCRF-CEM 人急性淋巴細(xì)胞白血病T淋巴細(xì)胞T淋巴母細(xì)胞形態(tài)：淋巴母細(xì)胞添加 2 mM L-谷氨酰胺，10 mM HEPES,1 m...                                                       

QY-x2340 Calu-3 人肺腺癌 (胸水)含 1.5g/L 碳酸氫鈉，0.1mM 非必需氨基酸，1.0mM 丙酮酸鈉，2mM L-谷氨酰胺和Earle&...                                                       

QY-x2341 Caki-1 人腎透明細(xì)胞癌皮膚轉(zhuǎn)移細(xì)胞McCoy's 5a 培養(yǎng)基，添加1.5 mM L-谷氨酰胺, 90%; FBS, 10% ...                                                 

QY-x2342 Caco-2 人直腸腺癌細(xì)胞EMEM培養(yǎng)基，添加2 mM L-谷氨酰胺和Earle's BSS，含1.5 g/L 碳酸氫鈉, 0.1 mM 非必需氨...                                                      

QY-x2343 CA46Burkitts淋巴癌細(xì)胞系RPMI 1640+20%胎牛血清或新生牛血清. ： The cells are EBNA ...