檢測標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等

檢測方法：ELISA

檢測類型：酶聯(lián)免疫夾心法

產(chǎn)品的用途：僅供科研究課題使用

價格及詳細(xì)資料：齊一生物銷售：021－6034 8496；181214 53965；173021 04490；.齊一生物科技（上海）有限公司提供的ELISA試劑盒受到了廣大科研單位的一致肯定和認(rèn)同.大品牌保證,價格公道,傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供*優(yōu)質(zhì)的產(chǎn)品.若有需要,我司將竭誠為您服務(wù)！本試劑盒用于測定血清,血漿及相關(guān)液體樣本中含量.

【雞銅藍(lán)蛋白(CP/CER)ELISA試劑盒現(xiàn)貨】實(shí)驗(yàn)原理：

   本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中該產(chǎn)品水平.用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色.TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成*終的黃色.顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān).用酶標(biāo)儀在450nm波長下測定吸光度（OD值）,通過標(biāo)準(zhǔn)曲線計算樣品中該產(chǎn)品濃度.

試劑盒組成：

【雞銅藍(lán)蛋白(CP/CER)ELISA試劑盒現(xiàn)貨】標(biāo)本要求：

1．標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn).若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融

2．不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的（HRP）活性.

操作步驟：

1.     標(biāo)準(zhǔn)品的稀釋與加樣：在酶標(biāo)包被板上設(shè)標(biāo)準(zhǔn)品孔10孔,在、第二孔中分別加標(biāo)準(zhǔn)品100μl,然后在、第二孔中加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后從孔、第二孔中各取100μl分別加到第三孔和第四孔,再在第三、第四孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻；然后在第三孔和第四孔中先各取50μl棄掉,再各取50μl分別加到第五、第六孔中,再在第五、第六孔中分別加標(biāo)準(zhǔn)品稀釋液50ul,混勻；混勻后從第五、第六孔中各取50μl分別加到第七、第八孔中,再在第七、第八孔中分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第七、第八孔中分別取50μl加到第九、第十孔中,再在第九第十孔分別加標(biāo)準(zhǔn)品稀釋液50μl,混勻后從第九第十孔中各取50μl棄掉.（稀釋后各孔加樣量都為50μl,濃度分別為1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.

2.     加樣：分別設(shè)空白孔（空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同）、待測樣品孔.在酶標(biāo)包被板上待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl（樣品*終稀釋度為5倍）.加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻.

3.     溫育：用封板膜封板后置37℃溫育30分鐘.

4.     配液：將30（48T的20倍）倍濃縮洗滌液用蒸餾水30（48T的20倍）倍稀釋后備用.

5.     洗滌：小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干.

6.     加酶：每孔加入酶標(biāo)試劑50μl,空白孔除外.

7.     溫育：操作同3.

8.     洗滌：操作同5.

9.     顯色：每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.

10. 終止：每孔加終止液50μl,終止反應(yīng)（此時藍(lán)色立轉(zhuǎn)黃色）.

11. 測定：以空白空調(diào)零,450nm波長依序測量各孔的吸光度（OD值）. 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行.

【雞銅藍(lán)蛋白(CP/CER)ELISA試劑盒現(xiàn)貨】注意事項(xiàng)：

1． 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存.

2． 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果.

3． 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗(yàn)誤差.一次加樣時間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排搶加樣.

4． 請每次測定的同時做標(biāo)準(zhǔn)曲線,做復(fù)孔.如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值）,請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定,計算時請*后乘以總稀釋倍數(shù)（×n×5）.

5． 封板膜只限一次性使用,以避免交叉污染.

6． 底物請避光保存.

7． 嚴(yán)格按照說明書的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理.

9． 本試劑不同批號組分不得混用.

10. 如與英文說明書有異,以英文說明書為準(zhǔn).

【雞銅藍(lán)蛋白(CP/CER)ELISA試劑盒現(xiàn)貨】計算：

以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),   

在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD     

值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度；再乘以稀釋     

倍數(shù)；或用標(biāo)準(zhǔn)物的濃度與OD值計算出標(biāo)     

準(zhǔn)曲線的直線回歸方程式,將樣品的OD值     

代入方程式,計算出樣品濃度,再乘以稀釋

倍數(shù),即為樣品的實(shí)際濃度.

試劑盒性能：

1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上.

2.批內(nèi)與批見應(yīng)分別小于9%和11%    

保存條件及有效期：

1.試劑盒保存；2-8℃.

2．有效期：6個月

130907     NeXtal DWBlock Anions Suite           CNY                             

4559263  artus C.trachomatisPlus RG PCR Kit(24)CE                CNY                             

4601203  artus Malaria RG PCR Kit (24) RUO          CNY                             

4843024  pg HPV PCR ASSAY            CNY                             

130826     NeXtal CubicPhase 3w Mo/C 8% µplate (10)            CNY                             

130727     NeXtal Tubes JCSG Core IV Suite                CNY                             

130703     NeXtal Tubes Cryos Suite          CNY                             

129916     QIAGEN RNase Inhibitor (500 reactions)          "QIAGEN RNase Inhibitor is a protein of nonhuman origin that binds noncovalently to an inactivates a variety of ribonucleases (e.g., RNase A, B, ａnd C). It does not interfere with RNase T1,T2, H, U1, U2, or CL3. QIAGEN RNase Inhibitor is a polyclonal antibody which is highly active ａnd inhibits more than 90% of RNase activity, whereas HPRI (human placental RNase inhibitor) inhibits only 50%. For this reason, it can be used at lower concentrations compared to other RNase

inhibitors. In addition, QIAGEN RNase Inhibitor is stable over a wide pH ａnd temperature range ａnd at varying dithiothreitol (DTT) concentrations. QIAGEN RNase Inhibitor is supplied at a concentration of 4 units per microliter in 2 mM KH2PO4, 8.0 mM Na2HPO4, 3.0 mM KCl, 150 mM NaCl, pH 7.4, ａnd 50% glycerol."  CNY                             

129115     Nuclease-Free Water (1000 ml)                CNY                             

4504263  artus Parvo B19 RG PCR Kit (24) CE                   CNY                             

4506203  artus HBV RG PCR Kit (24) RUO                CNY                             

4509005  artus WNV LC RT-PCR Kit (96) RUO          CNY                             

80311       AllPrep DNA/RNA 96 Kit (4)              CNY                              【雞銅藍(lán)蛋白(CP/CER)ELISA試劑盒現(xiàn)貨】

76554       RNAprotect Animal Blood Tubes 500µl (50)              CNY                             

76163       RNAlater TissueProtect Tubes (20x5ml)  For stabilization of RNA in 20 x 500 mg tissue samples: 20 screw-top tubes containing 5 ml RNAlater RNA Stabilization Reagent each     CNY                             

74182       RNeasy 96 Kit (12)   For 12 x 96 total RNA preps: 12 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, RNase-free Reagents ａnd Buffers       CNY                             

74136       RNeasy Plus Mini Kit (250)                 CNY                             

28004       MinElute PCR Purification Kit (50)   50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)    CNY                             

74124       RNeasy Protect Mini Kit (50)   RNAlater RNA Stabilization Reagent (50 ml), 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml ａnd 2 ml), RNase-free Reagents ａnd Buffers       CNY                             

19588       Elution Microtubes CL (24 x 96)                 CNY                             

19133       QIAGEN Proteinase K (10)        10 ml (>600 mAU/ml, solution)  CNY                             

19076       Buffer ATL         200 ml Tissue Lysis Buffer for 1000 preps         CNY                             

19048       EndoFree Plasmid Buffer Set   Buffers P1, P2, P3, QBT, QC, QN, ER, TE, Endotoxin-free water, RNase A; for 10  plasmid mega- or 5 gigapreps (endotoxin-free)    CNY                             

12961       QIAGEN Plasmid Plus Maxi Sample Kit (5)        QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega ａnd Giga) or up to 24 (Midi ａnd Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction.      CNY                             

12243       QIAfilter Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi Cartridges      CNY                             

12123       QIAGEN Plasmid Mini Kit (25) 25 QIAGEN-tip 20, Reagents, Buffers      CNY                             

133276     NeXtalStock Spermine (100)             CNY                             

4503065  artus CMV LC PCR Kit (96) CE            CNY                             

6000-5000        Specimen Collection Tubes                CNY                              【雞銅藍(lán)蛋白(CP/CER)ELISA試劑盒現(xiàn)貨】

5000-1430        MST Vortexer II - FoamFeetAssembly, 110V             CNY                             

5128-1220        Specimen Transport Medium (STM) CE             CNY                             

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QY-x2424 人T淋巴細(xì)胞白血病細(xì)胞 Jurkat 詢價 ATCC                                                  

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