1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒現(xiàn)貨】齊一生物【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測。避免給您帶來不必要的損失，請仔細(xì)閱讀購買說明！

ELISA應(yīng)用：

ELISA檢測試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測試劑盒、酶聯(lián)免疫吸附測定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗是一種敏感性高,特異性強(qiáng),重復(fù)性好的實驗診斷方法.由于其試劑穩(wěn)定、易保存,操作簡便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗的各領(lǐng)域中.ELISA檢測試劑盒適用于體外定性檢測人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測

【雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒現(xiàn)貨】齊一生物科技?xì)g迎您訂購咨詢，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

齊一生物供應(yīng)的ELISA試劑盒安全性提示：

1.工作人員實驗中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請盡快用水沖洗。

2.實驗中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個月

3.-50℃以下的冰箱，可以放一年

【雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒現(xiàn)貨】齊一生物供應(yīng)的ELISA試劑盒ELISA實驗原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將生物素標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測得HBsAg變異樣品,提高檢測的特異性（99.98%）提高檢測的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測試劑盒應(yīng)用定性夾心免疫檢測技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時會發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測量O.D.值,按照本HEVIgM抗體elisa試劑盒的測試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性. 【雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒現(xiàn)貨】

以上資料僅供參考了解，不供于論文寫作

齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費(fèi)代測）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測試劑盒、比色法檢測試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490

9241148  Transport lock, Y axis, M48               CNY                             

9241143  Application AUTOPURE (customized)                CNY                             

9241005  QIArent&go - Rent out fee BR Universal                   CNY                             

9240987  Kit, electronic, FFC-plugs, Qsym                CNY                             

9240980  Sensor, tray, sample tracking, Qsym                  CNY                             

9240945  Sensor, xy, portal, Qsym            CNY                             

9234856  Tubing coupling piece for washer liquid            CNY                             

9234852  Card, ISDN, Fritz PCI                  CNY                             

9240783  Update, Weigh Cell, AP96                  CNY                             

9240774  Assy, Transfer Rack, AP96                  CNY                             

9241632  LED Light System, AP                 CNY                              【雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒現(xiàn)貨】

9241369  Temperature Controller, EZ1a                   CNY                             

9243645  Guided Valid Support, QIA Extraction Sys         The QIAGEN Other Extraction System Guided Validation Support service includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to QIAGEN extraction chemistry utilized in the process, review the appropriate Handbook ａnd User Manual, as well as hands-on chemistry ａnd instrument operation as required.  The Guided Validation service includes experimental design consultation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Biosprint 96, DNA mericon)         CNY                             

9243542  BR MDx, PM Service        "Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"  CNY                             

9243305  Power Cord, India             CNY                             

9243110  CD-RW, Black, AP196/98           CNY                             

9242712  Validation Support, 2d QIAcube       The QIAGEN QIAcube Guided Validation includes all travel ａnd labor charges for a site visit up to 2 days by a QIAGEN Specialist. A QIAGEN specialist will provide an introduction to the QIAcube system ａnd the DNA Investigator silica chemistry utilized in the process, review the Handbook ａnd User Manual, as well as hands-on QIAcube operation.  The Guided Validation service includes experimental design consulation, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support.  Please indicate which protocols to include in the validation (QIAamp, QIAamp DNA Investigator, MinElute PCR Cleanup, etc.)      CNY                             

9019226  Fan - rear 92x92, PyroQ24                 CNY                             

9019220  PCB - Distribution robot, PyroQ96MD               CNY                             

1066843  PG Influenza A RT-PCR Assay (48) LE Z              CNY                             

1066848  NDV Univ. RT-PCR REAGENT LE                 CNY                             

1066854  AIV-H9 RT-PCR REAGENT LE             CNY                             

1060992  Buffer MW1 (77ml)           CNY                             

1061059  Sample Preparation Reagents, 8ml                   CNY                             

1061080  NG Quantification Standards            CNY                             

1061113  PG AIV-H7 RT-PCR Assay (48) Z                  CNY                             

9016568  Plate Grippers, RCS1                  CNY                              【雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒現(xiàn)貨】

9016536  Transportation box , BioSprint 15             CNY                             

9016535  Transportation box , Twister II                   CNY                             

9016533  Transportation box , RapidPlate onlf.                CNY                             

9016530  Transportation box 1, LiquiChip                 CNY                             

QY-x2344 Ca Ski 人宮頸癌腸轉(zhuǎn)移細(xì)胞RPMI 1640培養(yǎng)基，添加2 mM L-谷氨酰胺，含1.5 g/L 碳酸氫鈉, 4.5 g/L 葡萄糖, 10 m...                                                        

QY-x2345 C6大鼠膠質(zhì)瘤細(xì)胞 含有2mM L-谷氨酰胺的F12K，加1.5克/升碳酸氫鈉，添加15%馬血清，2.5%胎牛血清。 ： 膠質(zhì)細(xì)胞株C6是...                                                 

QY-x2346 C2C12小鼠成肌細(xì)胞mouse組織                                               

QY-x2347 BxPC-3 人原位胰腺腺癌細(xì)胞 含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 10mM HEPES，1.0mM 丙酮酸鈉和2mM L-谷氨酰胺的RPM...                                                 

QY-x2348 BT-549 人乳腺管癌細(xì)胞 含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 10mM HEPES, 2mM L-谷氨酰胺和0.023單位/ml胰島素...                                                  

QY-x2349 BHK-21 倉鼠幼倉鼠腎細(xì)胞RPMI 1640或DMEM + 20% 胎牛血清或新生牛血清. ： -                                                     

QY-x2350 BGC-823人胃腺癌細(xì)胞（低分化）/62RPMI 1640 + 10% 胎牛血清或新生牛血清. ： 建自一位62歲的胃癌患者。                                                    

QY-x2351 BEL-7402 人肝癌細(xì)胞 RPMI 1640 + 10% 胎牛血清或新生牛血清。 ： BEL-7402細(xì)胞株是1974年從臨床肝癌手術(shù)標(biāo)本中建立的。 細(xì)...                                                    

QY-x2352 B16 小鼠黑色素瘤細(xì)胞RPMI 1640 + 10% 胎牛血清或新生牛血清. ： 小鼠黑素瘤細(xì)胞。                                                     

QY-x2353 AsPC-1 人轉(zhuǎn)移胰腺腺癌細(xì)胞 含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 10mM HEPES，1.0mM 丙酮酸鈉和2mM L-谷氨酰胺的...                                                   

QY-x2354 Ana-1小鼠巨噬細(xì)胞RPMI 1640 + 10% 胎牛血清或新生牛血清。 ： -                                                        【雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒現(xiàn)貨】

QY-x2355 AAV-293人胚腎細(xì)胞含4.5g/L葡萄糖，110mg/L丙酮酸鈉，4mM L-谷氨酰胺的DMEM，90%；熱滅活胎牛血清 1...                                                        

QY-x2356 A549 [A-549]人肺癌細(xì)胞 含2mM L-谷氨酰胺的F12K，加1.5克/升碳酸氫鈉，添加10%胎牛血清。 ： 該細(xì)胞系由D.J.Griad通過肺癌...                                                  

QY-x2357 A-431人表皮癌細(xì)胞 含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖，4mM L-谷氨酰胺的DMEM，90%；胎牛血清或新生牛血清，10%。...                                                

QY-x2358 A-375[A375] 人惡性黑色素瘤細(xì)胞含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖，4mM L-谷氨酰胺的DMEM，90%；胎牛血清或新...                                                      

QY-x2359 A3人T淋巴細(xì)胞白血病細(xì)胞，T淋巴細(xì)胞形態(tài)： 淋巴母細(xì)胞含2mM L-谷氨酰胺，10mM HEPES，1mM丙酮酸鈉，4.5g/L葡萄糖和1.5g/L 碳酸氫鈉的R...                                                  

QY-x2360 95-D人高轉(zhuǎn)移肺癌細(xì)胞RPMI 1640，90%；胎牛血清或新生牛血清 10%。 ： 這是一株高轉(zhuǎn)移肺癌。                                                       

QY-x2361 786-O人腎透明細(xì)胞腺癌細(xì)胞 含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 10mM HEPES，1.0mM丙酮酸鈉，和2mM L-谷氨酰胺...                                                   

QY-x2362 6T-CEM 人T細(xì)胞白血病細(xì)胞急性淋巴細(xì)胞白血病形態(tài)： 淋巴母細(xì)胞RPMI 1640 + 10% 胎牛血清或新生牛血清。 ： 這是一個CCRF-CEM的6-硫... 小鼠脂肪細(xì)胞 3T3-L1 詢價 ATCC                                                

QY-x2363 3T3-L1 小鼠脂肪細(xì)胞含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖，4mM L-谷氨酰胺的DMEM，90%；BCS，10%。 ： L1是通過... 人胚腎細(xì)胞 293T 詢價 ATCC                                                  

QY-x2364 293T人胚腎細(xì)胞293T含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 4mM L-谷氨酰胺的DMEM，90%；胎牛血清，10%。 ： 293細(xì)胞株插入...                                                

QY-x2365 22RV1人前列腺癌細(xì)胞 含10mM HEPES, 1mM 丙酮酸鈉，4.5g/l 葡萄糖和1.5g/l碳酸氫鈉和2mM L-谷氨酰胺的RPMI 1640;                                                    

QY-x2366 5637人膀胱癌細(xì)胞 膀胱；癌形態(tài)： /年齡： 含1.5g/L 碳酸氫鈉， 4.5g/L 葡萄糖， 10mM HEPES，1.0mM丙酮酸鈉，和2mM L-谷氨酰胺的RPMI 16...                                                  

QY-x2367 293 人胚腎細(xì)胞腎；以腺病毒5 DNA 進(jìn)行轉(zhuǎn)化。 含 1.5g/L 碳酸氫鈉，0.1mM 非必需氨基酸，1.0mM 丙酮酸鈉，2mM L-谷氨酰胺和Earle&...                                                       

QY-x2368 大鼠肝細(xì)胞BRL                                                   

QY-x2369 人肝母細(xì)胞瘤細(xì)胞HUH-6                                                   

QY-x2370 小鼠淋巴瘤細(xì)胞YAC-1                                                 【雞可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA試劑盒現(xiàn)貨】

QY-x2371 人羊膜細(xì)胞WISH                                                

QY-x2372 人胚肺細(xì)胞WI-38                                               

QY-x2373 非洲綠猴腎細(xì)胞Vero