1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說(shuō)明！【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現(xiàn)貨】

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見(jiàn)的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問(wèn)世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現(xiàn)貨】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現(xiàn)貨】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過(guò)的HRP.再經(jīng)過(guò)溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開(kāi)優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過(guò)各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說(shuō)（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開(kāi)放閱讀框2和開(kāi)放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過(guò)氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性.

以上資料僅供參考了解，不供于論文寫(xiě)作

【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現(xiàn)貨】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

9238645 Brake resistor 112 Ohm / 100 W      CNY            

9242061 Pipetting container, IQ/OQ, QS      CNY            

9241827 Pyro Q96 ID, Premium Agreement  "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9241166 Board, A/D, QX      CNY            

9240955 Sensor, z, handler, Qsym        CNY            

9240835 QIAcube, Basic Agreement, no PM The Basic Agreement QIAcube provides full service coverage for the QIAcube. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Basic Agreement, there is a priority response time of 5  working days.   CNY            

9240766 32 OUT Module, D2-32TD1, AP96       CNY            

6000-5018   MST STM Rack & Lid (Blue - 2 pos. cal.)     CNY            

9241777 QIAxtractor, Inspection Service "The Annual Inspection Service, QIAxtractor is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAextractor are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report."    CNY            

9241375 Magnet, door lock, top, EZ1a        CNY            

9241334 Keypad foil, EZ1        CNY            

9240750 Belt, Elevator, AP      CNY            

9243648 Acceler. Valid Supp, Other Extract Sys  The QIAGEN Accelerated Validation Support Other Extraction System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Specialist.  A QIAGEN specialist will provide provide an introduction to the instrument ａnd hands-on user support.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored data templates.  The QIAGEN Specialist will support the customer by running samples through the QIAGEN extraction method/system, as well as general wet lab support such as pipetting for quantification, STR ａnd CE setup, normalization, etc. to produce the data needed for the validation study.  The customer is responsible for submitting the data to QIAGEN for assisted data analysis. The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product.  Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included by QIAGEN.  Attendees will receive a certificate of participation in the hands-on exercises.  Please indicate which protocols to validate (Biosprint 96, DNA mericon).    CNY            

9243553 #BR M96, PM Service "

Service response time (repair): not applicable

Periodic inspection/maintenance: yes

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts : not included

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY             【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現(xiàn)貨】

9242864 QIArent&go -Rent out fee QSSP/AS Cabinet        CNY            

5000-1321   Feet Set/4, SKR1        CNY            

5000-1288   DML 2000 - Fuse 160 mA PKG 10       CNY            

5000-1287   DML 2000 - Fuse 315 mA PKG 10       CNY            

5000-1505   Switch ON-OFF 120V, HTR1        CNY            

6000-00181  Plate Washer - Replacement Tubing Kit       CNY            

6000-00178  Plate Washer - Maintenance Kit      CNY            

5100-00047  Wash Buffer Concentrate (2 Bottles) "2 bottles of 100mL each.

The price is proportional to 2x 5100-0795 (discontinued)"   CNY            

9240740 Tubing, Tygon, 1/4" ID      CNY            

9240735 Tubing, Black, Capper, AP196/98     CNY            

9240724 Tube Assy, Draw, AP96/196       CNY            

5000-1412   Poteneiometer 500K, VTX2        CNY            

9240506 Monitor, 17", Flat Panel, AP        CNY            

9239851 Cover, LC200        CNY            

9239565 xMAP Alignment Microspheres     CNY            

9019942 Barrel removal tool, CX1        CNY             【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現(xiàn)貨】

9019474 Lid, UV, polycarb, C1x      CNY            

QY-x1954    COLO 205 結(jié)腸癌 1640+10%胎牛血清                        【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現(xiàn)貨】

QY-x1955    COLO320DM 結(jié)腸癌 1640+10%胎牛血清                      

QY-x1956    HCT-8 回盲腸腺癌 1640+10%胎牛血清                      

QY-x1957    HCT-116 低分化結(jié)腸腺癌 1640+10%胎牛血清                    

QY-x1958    HT-29 結(jié)腸腺癌 1640+10%胎牛血清                    

QY-x1959    LS-174T 結(jié)腸腺癌 MEM+非必需氨基酸+10%胎牛血清                      

QY-x1960    SW480 結(jié)腸癌 L15                       

QY-x1961    BEL-7402 肝細(xì)胞癌 1640+10%胎牛血清                     

QY-x1962    BEL-7405 肝細(xì)胞癌 1640+10%胎牛血清                     

QY-x1963    H7402 肝癌 1640+10%胎牛血清                    

QY-x1964    HCC-9204 肝癌 1640+10%胎牛血清                     

QY-x1965    Hep3B 肝癌 DMEM+非必需氨基酸+10%胎牛血清                       

QY-x1966    Hep G2 肝癌 DMEM+10%胎牛血清                       

QY-x1967    SMMC-7721 肝癌 1640+10%胎牛血清                    

QY-x1968    PANC-1 胰腺導(dǎo)管上皮癌 1640+10%胎牛血清                     

QY-x1969    Panc 10.05 胰腺腺癌 高糖1640+15%胎牛血清+胰島素等                      

QY-x1970    SW1990 胰腺腺癌 1640+10%胎牛血清                        【雞血小板因子4(PF-4/CXCL4)ELISA試劑盒現(xiàn)貨】

QY-x1971    JF305 胰腺癌 1640+10%胎牛血清                      

QY-x1972    PC-3 胰腺癌 1640+10%胎牛血清                       

QY-x1973    Capan-2 胰腺癌 1640+10%胎牛血清                    

QY-x1974    SK-RC-42 腎癌 1640+10%胎牛血清                     

QY-x1975    95-C 低轉(zhuǎn)移肺癌 1640+10%胎牛血清                       

QY-x1976    95-D 高轉(zhuǎn)移肺癌 1640+10%胎牛血清                       

QY-x1977    973 肺腺癌 1640+10%胎牛血清                    

QY-x1978    A2 肺腺癌 1640+10%胎牛血清                     

QY-x1979    A549 肺腺癌 1640+10%胎牛血清                       

QY-x1980    A549/DDP 肺腺癌/順鉑耐藥株 1640+10%胎牛血清                    

QY-x1981    Calu-3 肺腺癌 MEM+非必需氨基酸+10%胎牛血清                     

QY-x1982    GLC-82 肺腺癌 1640+10%胎牛血清 已有                    

QY-x1983    H1299 非小細(xì)胞肺腺癌 1640+10%胎牛血清