1. 酶標(biāo)儀(450nm 波長(zhǎng)濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來(lái)不必要的損失，請(qǐng)仔細(xì)閱讀購(gòu)買說明！【雞白血病抑制因子(LIF)ELISA試劑盒現(xiàn)貨】

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問世以來(lái),得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國(guó)獲得很大的推廣,尤其是國(guó)內(nèi)生化領(lǐng)域的長(zhǎng)足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【雞白血病抑制因子(LIF)ELISA試劑盒現(xiàn)貨】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【雞白血病抑制因子(LIF)ELISA試劑盒現(xiàn)貨】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來(lái),隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性?shī)A心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國(guó)型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來(lái)自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長(zhǎng):450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽(yáng)性.

以上資料僅供參考了解，不供于論文寫作

【雞白血病抑制因子(LIF)ELISA試劑盒現(xiàn)貨】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購(gòu)！

9236424 Motor, rotary, TWI      CNY            

9232970 YZ 96 Connector Board 2 (Arm), E10103       CNY            

9236409 Plate Stacker TW I, microplate      CNY            

9236357 Sample Holder Large, 1.5ml      CNY            

9232911 Board, YZ driver        CNY            

9236336 Analog Board        CNY            

9236158 Pot. , syr. or vert. , kit, RapidPlate      CNY            

9236149 Locator Plate, 96 well,pos 4-5,BR RP        CNY            

9236114 O-Ring, Coupling, RoboVac, BR8 RD v4.2      CNY             【雞白血病抑制因子(LIF)ELISA試劑盒現(xiàn)貨】

9236107 Flange Tool     CNY            

9230680 Probe, 0.4 mm, BR8000 v1.01     CNY            

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9230473 Set, Cables, PCB (X-main) to shaker     CNY            

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9235255 T-piece, HSPS tubing, connector     CNY            

9235190 Motor, clamp, shaker plate,  BR8000     CNY            

9230060 Rack for reagents (12 x diameter 21mm)      CNY            

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9238653 Power Board, temp. switch - heat sink       CNY            

9238635 Hook identification compl.      CNY            

9242028 RGQ, Repair Fee "Repair service delivery send to Regional Repair Center

Service response time (repair) 7-10 days turnaround time

Periodic inspection/maintenance: 0

Cost coverage for Repair Parts : yes, full coverage

Cost coverage for labor: yes, full coverage

Cost coverage for travel: yes, full coverage

Replacement system (Loaner) provision: not applicable

Transportation cost coverage: yes, full coverage"   CNY            

9241821 Pyro Q96 MDa, Basic Agreement   "Repair service delivery: onsite

Service response time (repair): 5 business days

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"   CNY            

9241786 QIAgility Demopool Box Light Version        CNY            

9240976 PCB, display, sample tracking, Qsym     CNY            

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9240816 #Full Agreement, EZ1 DSP, 1YR       CNY            

9240680 Spill Tray, Shaker,Modified, AP     CNY            

9240790 Update,Waste Bottle Sensor, AP196/98        CNY            

6000-5014   Lumicheck Plate Service Package, DML2       CNY            

9241770 QIAxtractor, Premium Agreement  The Premium Cover Agreement, QIAxtractor provides full service coverage for the QIAxtractor ａnd the CAS 1280. Costs for service visits are fully covered including travel, labor, ａnd parts. One on-site pipetting head service ａnd inspection is included. With a Premium Cover Agreement, there is a priority response time of 24 hours (1 working day).    CNY            

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QY-x881 L-02人胎肝細(xì)胞系                       

QY-x882 Hut-102人T淋巴瘤細(xì)胞系                    

QY-x883 MCF-7人乳腺癌細(xì)胞系                    

QY-x884 ZR-75-30人乳腺癌細(xì)胞系                     

QY-x885 PK-15豬腎細(xì)胞系                    

QY-x886 IBRS-2豬腎細(xì)胞系                       

QY-x887 ST豬睪丸細(xì)胞系                      【雞白血病抑制因子(LIF)ELISA試劑盒現(xiàn)貨】

QY-x888 Sf-21昆蟲細(xì)胞系                    

QY-x889 SF-9昆蟲細(xì)胞系                     

QY-x890 L8824草魚肝臟細(xì)胞                      

QY-x891 CIK草魚腎臟細(xì)胞                    

QY-x892 WCF團(tuán)頭魴尾鰭細(xì)胞                      

QY-x893 SCF白鰱尾鰭細(xì)胞                    

QY-x894 SiHa人宮頸鱗狀細(xì)胞癌                       

QY-x895 Ski人宮頸上皮細(xì)胞癌                    

QY-x896 SW626人卵巢腺癌細(xì)胞                    

QY-x897 BI U－87膀胱癌細(xì)胞                     

QY-x898 C6/36蚊子細(xì)胞                      

QY-x899 HBZY－1大鼠腎小球系膜細(xì)胞                      

QY-x900 AN3 CA人子宮內(nèi)膜腺癌細(xì)胞                       

QY-x901 H4人腦神經(jīng)膠質(zhì)瘤細(xì)胞                       

QY-x902 HEC－1－B 人子宮膜腺癌細(xì)胞                     

QY-x903 MADB106大鼠乳腺腺癌細(xì)胞系                       【雞白血病抑制因子(LIF)ELISA試劑盒現(xiàn)貨】

QY-x904 人口腔表皮樣癌細(xì)胞KB                       

QY-x905 人口腔上皮癌長(zhǎng)春新堿耐藥株KB/V                     

QY-x906 人口腔癌細(xì)胞HB                     

QY-x907 人喉表皮樣癌細(xì)胞HEp-2                      

QY-x908 人咽鱗癌細(xì)胞FaDu                       

QY-x909 人喉癌細(xì)胞TU 686                       

QY-x910 人喉癌細(xì)胞TU212