齊一生物銷(xiāo)售：021－6034 8496；181214 53965；173021 04490

1。我必須運(yùn)行所有的標(biāo)準(zhǔn)和樣品的重復(fù)？

是的，重復(fù)的運(yùn)行，以監(jiān)測(cè)測(cè)定的精度和提高檢測(cè)結(jié)果的信心。

2。我必須在一次運(yùn)行所有的樣品嗎？

不，每個(gè)試劑盒采用stripwell微孔板。這允許用戶(hù)在不同的時(shí)間來(lái)分析不同數(shù)量的樣品。

3。什么類(lèi)型的可重復(fù)性的結(jié)果，得到與分析？

每一個(gè)工具都有一個(gè)包含一個(gè)典型數(shù)據(jù)的圖表的手冊(cè)。在運(yùn)營(yíng)商的任何變化，移液和洗滌技術(shù)，培養(yǎng)時(shí)間、溫度、試劑盒的年齡可以引起變化的結(jié)果。每個(gè)用戶(hù)都應(yīng)該得到自己的標(biāo)準(zhǔn)曲線(xiàn)。

4。是否有可能儲(chǔ)存的試劑比表示？

在不推薦使用條件下的部件組件的存儲(chǔ)，以保證測(cè)試的正確性能。

5。我應(yīng)該如何儲(chǔ)存我的樣品？

樣品應(yīng)保存在-20°C或更低的溫度。長(zhǎng)期儲(chǔ)存，建議凍結(jié)他們?cè)?/span>- 70余。

6。我可以修改協(xié)議嗎？

BG的ELISA試劑盒進(jìn)行了優(yōu)化，提供*佳的結(jié)果。修改的格式或協(xié)議可能會(huì)給出錯(cuò)誤的結(jié)果。

7。我可以使用一個(gè)不建議在試劑盒插入的樣本類(lèi)型嗎？

該試劑盒已被驗(yàn)證在試劑盒插入的樣品類(lèi)型。未經(jīng)檢驗(yàn)的樣品類(lèi)型。為進(jìn)一步信息的聯(lián)系技術(shù)服務(wù)。

8。我的樣本產(chǎn)生的值，在動(dòng)態(tài)范圍內(nèi)的測(cè)定。我可以使用這些值嗎？

建議只有在標(biāo)準(zhǔn)曲線(xiàn)的范圍內(nèi)下降的樣本值。標(biāo)準(zhǔn)曲線(xiàn)的范圍內(nèi)的值一般是非線(xiàn)性的，這可能導(dǎo)致不正確的外推值。產(chǎn)生值高于標(biāo)準(zhǔn)的樣品應(yīng)為（進(jìn)一步）稀釋和重復(fù)。如果樣品低于該測(cè)定的范圍內(nèi)，該樣品被認(rèn)為是不可檢測(cè)的。

9。我每次都要運(yùn)行一個(gè)空白或零標(biāo)準(zhǔn)嗎？

是的，這些都是必需的計(jì)算，并反映任何微妙但顯著的性能變化，從一天到一天，檢測(cè)到檢測(cè)。當(dāng)排除特定的檢測(cè)問(wèn)題的來(lái)源時(shí)，它們也非常有幫助。

10。我能改變我在檢測(cè)中使用的樣本量嗎？

不建議你改變卷由于BG工具被設(shè)計(jì)為*佳的性能在給定的體積

11�？梢允褂貌煌慕M件組件嗎？

每一個(gè)組件包含有特定批號(hào)的組件，以確保所有的組件都進(jìn)行優(yōu)化，以及與組件中的所有其他組件。對(duì)這些特定的地段進(jìn)行質(zhì)量檢驗(yàn)。這是從來(lái)沒(méi)有建議使用你自己的組件或組件從其他包或供應(yīng)商。

12。我的標(biāo)準(zhǔn)曲線(xiàn)看起來(lái)很好，但我沒(méi)有得到一個(gè)信號(hào)，我的樣本，當(dāng)我預(yù)期，為什么？

樣品不包含被分析物。一個(gè)矩陣的效果可能是掩蓋檢測(cè)。確保推薦的稀釋液在試劑盒插入后按規(guī)定進(jìn)行。如果建議稀釋?zhuān)?qǐng)檢查是否正確進(jìn)行稀釋。過(guò)稀釋會(huì)導(dǎo)致樣品在標(biāo)準(zhǔn)曲線(xiàn)的范圍內(nèi)下降。

13。你怎么推薦我洗盤(pán)子？

如果您使用的是一個(gè)自動(dòng)平板洗衣機(jī)，我們建議定期檢查校準(zhǔn)，并將該系統(tǒng)在洗滌前沖洗緩沖液沖洗。手動(dòng)洗衣機(jī)也是一樣的。一個(gè)中繼器或洗瓶也可以使用。用戶(hù)應(yīng)小心，以確保所有的內(nèi)容都是送氣和板拍干的無(wú)塵紙。【雞白介素2(IL-2)ELISA試劑盒現(xiàn)貨】

14。我需要用一個(gè)盤(pán)子嗎？

可靠的結(jié)果可以得到無(wú)板振動(dòng)，但外徑的一般會(huì)低于那些使用平板振動(dòng)器。

15。為什么我要用波長(zhǎng)校正450-570nm之間？

對(duì)于酶聯(lián)免疫吸附試驗(yàn)，在雙波長(zhǎng)讀的是做正確的光密度貢獻(xiàn)的塑料以及，燈和光學(xué)波動(dòng)。

16。如果我提我的樣本，我還需要遵循推薦的稀釋液試劑盒中插入了嗎？

提取過(guò)程中所需的樣本稀釋量將受到影響的純化和濃度的影響的協(xié)議使用。稀釋或濃縮的金額將由*終用戶(hù)決定。

17。什么是預(yù)期的分析物濃度，我應(yīng)該找到？

一個(gè)給定的分析物的量可能會(huì)有所不同，不僅從物種的物種，但也組織和細(xì)胞來(lái)源。這個(gè)信息的*佳來(lái)源是目前的文獻(xiàn)，很容易通過(guò)互聯(lián)網(wǎng)在多個(gè)科學(xué)數(shù)據(jù)庫(kù)訪(fǎng)問(wèn)。

18。我的光密度有點(diǎn)高（或較低），比那些在手冊(cè)中，與我的試劑盒來(lái)了。為什么？

光學(xué)密度受影響

1.    Do I have to run all of my standards ａnd samples in duplicate?

Yes, the duplicates are run in order to monitor assay precision ａnd increase confidence in the assay results obtained.

2.    Do I have to run all of my samples at one time?

No, each kit uses stripwell microplate. This allows the user to analyse different numbers of samples at different times.

3.    What types of reproducible results are obtained with the assays?

Each kit comes with a manual containing a graph of typical data obtained. Any variation in operator, pipetting ａnd washing technique, incubation time or temperature, ａnd kit age can cause variation in result. Each user should obtain their own standard curve.

4.    Is it possible to store the reagents other than indicated?

Storage of the kit components under conditions other than indicated is not recommended in order to assure proper performance of the test.

5.    How should I store my samples?

Samples should be stored at -20oC or lower temperature. For long-term storage, it is recommended to freeze them at -70oC -80oC.

6.    Can I modify the protocol?

BG ELISA kits have been optimized to provide the best possible results. Modifying the format or protocol may give inaccurate ａnd wrong results.

7.    Can I use a sample type that is not recommended in the kit insert?

The kit has been validated for the sample types listed in the kit insert. Sample types other than those validated have not been tested. Contact Technical Service for further information.

8.    My samples generated values that were outside the dynamic range of the assay. Can I use these values?

It is recommended that only sample values that fall within the range of the standard curve be used. Values outside the range of the standard curve are generally non-linear, which can lead to incorrectly extrapolated values. Samples that generate values higher than the highest standard should be (further) diluted ａnd the assay repeated. If samples fall below the range of the assay, the sample is considered to be non-detectable. 【雞白介素2(IL-2)ELISA試劑盒現(xiàn)貨】

9.    Do I have to run a Blank or Zero Standards every time?

Yes, these are required for the calculations, ａnd reflect any subtle but significant performance changes from day to day ａnd assay to assay. They are also extremely helpful when troubleshooting the source of a particular assay problem.

10.  Can I alter the volume of sample I use in the assay?

It is not recommended that you alter the volumes since all BG kits are designed for optimal performance at the given volumes

11.  Can components from different kits be used?

Each kit contains components which have specific lot numbers to ensure that all of the components are performing optimally alone, as well as with all of the other components in the kit. QC testing is performed on these specific lots. It is never recommended to use your own components or components from other kits or vendors.

12.  My standard curve looked fine, but I didn’t get a signal in my sample when I expected to, why?

The sample may not contain the analyte. A matrix effect may be masking the detection. Ensure that the recommended dilution was followed as stated in the kit insert. If dilution was recommended, check to be sure that the dilution was performed properly. Over-dilution may cause the sample to fall below the range of the standard curve.

13.  How do you recommend I wash my plate?

If you are using an automated plate washer we recommend that the calibration be checked on a regular basis, ａnd that the system is flushed with the Plate Washing Buffer prior to washing. The same is true for a manual washer. A repeater or a wash bottle can also be used. The user should be careful to ensure that all of the contents are aspirated ａnd the plate tapped dry on lint-free paper.

14.  Do I need to use a plate shaker?

Reliable results can be obtained without a plate shaker, but the O.D.＇s will generally be lower than those obtained using a plate shaker.

15.  Why do I have to use wavelength correction between 450-570nm?

For the ELISA assay, reading at dual wavelengths is done to correct for the optical density contributed by the plastic well, the lamp ａnd optical fluctuations. 【雞白介素2(IL-2)ELISA試劑盒現(xiàn)貨】

16.  If I extract my sample, do I still need to follow the recommended dilutions given in the kit insert?

The amount of sample dilution needed after an extraction procedure will be affected by the effects of purification ａnd concentration in the protocol used. The amount of dilution or concentration will have to be determined by the end-user.

17.  What is the expected concentration of analyte that I should expect to find?

The amount of a given analyte may vary not only from species-to-species, but also between tissue ａnd cellular sources. The best source of this information is the current literature that is easily accessed through the Internet at multiple scientific databases.

18.  My optical densities were a little higher (or lower) than those in the manual that came with my kit. Why?

The optical density is affected by a number of physical conditions such as time ａnd temperature. We suggest that you shorten or lengthen the final incubation with substrate solution to compensate.

19.  What are the reasons for High Background?

1)     Improper Washing: Check volume of washing buffer reservoir ａnd make sure all recommended washing steps are performed.

2)     Contaminated Substrate: Make sure there is no contamination of the substrate with metal ions or oxidizing reagents, before use. Keep the extra substrate solution separately during the ELISA substrate development time. 【雞白介素2(IL-2)ELISA試劑盒現(xiàn)貨】

3)     Substrate exposed to light: Exposure to light may result in a blue color of the substrate. Keep solutions in the dark (vial) until ready to dispense into the plate.

4)     Wrong Incubation Times/Temperatures: Generally follow the test protocol regarding incubation times ａnd temperatures. However, if all wells are intensely ａnd equally colored with no intensity gradient observed in the standard dilution series, then it may be necessary to observe the substrate reaction as the color is developing, in order to stop the reaction sooner.

9011779  Handle, HS shaker adapter plate, BR8000                CNY                             

9019086  PyroMark Q96 MD Software (5)      "For use with PyroMark Q96 MD instrument.Software for setting up a run ａnd analyzing mutations.

5 licenses"        CNY                             

9019068  PyroMark CpG SW 1.0 (5)         "Software for PyroMark Q96 ID ａnd PyroMark Q96 MD for analysis ａnd quantification of CpG methylation in epigenetic studies.

Five additional software licenses for PyroMark CpG SW 1.0" CNY                             

9019031  User Manual, EZ1xl          CNY                             

9019021  Damper, door, EZ1xl                  CNY                             

9019004  Grid, UV lamp, EZ1xl                 CNY                             

9019003  Hinge, for door, EZ1xl                CNY                             

9018951  RBlock, 16x0.2ml PCR 8x2ml/1.5ml Flat/Ta              CNY                             

9018930  Adapter, 33x2ml flat base Tubes               CNY                             

9022739  Rotor-Gene AssayManager SOW(10 licenses)          CNY                             

9022707  Retainer, PMT Baffle, DML3000               CNY                             

9016027  Extension set (Z), 4 x 50mm, Pos. Sys.               CNY                             

9015911  Connecting Tool, Disp.Head, BR8 V2.0             CNY                             

9015864  EZ1 FNSC Card Pre-programmed card for BioRobot EZ1 Forensic protocols   CNY                             

9015638  AC fuse, 115 V, 250 8A, M48             CNY                              【雞白介素2(IL-2)ELISA試劑盒現(xiàn)貨】

9015537  Window, for door              CNY                             

9019170  PCB 2 - Connection, PyroQ96MD              CNY                             

9019157  Cog belt kit - X/Y, PyroQ96MD          CNY                             

9014145  Rubber spring (10), Microplate Holder LH                CNY                             

9021254  PC, DCU             CNY                             

9021135  Power Supply Module 1 (U164), DCU                CNY                             

9021121  Valve Manifold, Gripper, DCU           CNY                             

9021054  Controller, Theta, DCU              CNY                             

9021031  Frame, transfer module, Qsym                  CNY                             

9021024  Lid, waste box, AS, Qsym                   CNY                             

9020962  Notebook, small instruments            CNY                             

9020729  Cooling adapter, MTP, RB, v2, Qsym                 CNY                             

9020710  Fan, electrical, 80mm, C34               CNY                             

9020702  Table plate, complete, C12               CNY                             

9016654  Microplate Station 8000, tall            CNY                             

9016584  Plate Stacker, RCS1           CNY                              【雞白介素2(IL-2)ELISA試劑盒現(xiàn)貨】

QY-x140   V79-4中國(guó)倉(cāng)鼠肺細(xì)胞  CCL-93                                          

QY-x141   CHL/IU [ CHL-11 ]中國(guó)倉(cāng)鼠肺細(xì)胞          CRL-1935                                               

QY-x142   BHK幼倉(cāng)鼠腎細(xì)胞          CCL-10                                           【雞白介素2(IL-2)ELISA試劑盒現(xiàn)貨】

QY-x143   R 1610中國(guó)倉(cāng)鼠體細(xì)胞         CRL-1657                                               

QY-x144   BRL 3A大鼠肝細(xì)胞         CRL-1442                                               

QY-x145   NRK大鼠腎細(xì)胞     CRL-6509                                               

QY-x146   L929成纖維細(xì)胞    CCL-1                                             

QY-x147   Y1腎上腺皮質(zhì)細(xì)胞        CCL-79                                          

QY-x148   CTLL-2T細(xì)胞   TIB-214                                        

QY-x149   BALB/3T3BALB/C小鼠胚成纖維細(xì)胞    CCL-163.2                                              

QY-x150   Ana-1巨噬細(xì)胞                                                    

QY-x151   SRSV/3T3SRSV轉(zhuǎn)化的3T3細(xì)胞                                                   

QY-x152   3T6-Swiss albino胚胎成纖維細(xì)胞           CCL-96                                          

QY-x153   PA317成纖維細(xì)胞           CRL-9078                                               

QY-x154   3T3-Swiss albino胚胎成纖維細(xì)胞           CCL-92                                          

QY-x155   Mo-MuLv/3T3Mo-MuLv感染的3T3細(xì)胞                                                     

QY-x156   MRC-5胚肺細(xì)胞     CCL-171                                        

QY-x157   WI-38胚肺細(xì)胞      CCL-75                                          

QY-x158   FRhK-4恒河猴胚腎細(xì)胞         CRL-1688                                                【雞白介素2(IL-2)ELISA試劑盒現(xiàn)貨】

QY-x159   VERO C1008 E6非洲綠猴腎細(xì)胞   CRL-1586                                               

QY-x160   Pt K1 NBL-3長(zhǎng)鼻袋鼠腎細(xì)胞          CCL-35                                          

QY-x161   L1210白血病細(xì)胞  CCL-219                                        

QY-x162   Neuro-2A腦神經(jīng)瘤細(xì)胞         CCL-131                                        

QY-x163   PC-12腎上腺嗜鉻細(xì)胞瘤細(xì)胞       CRL-1721                                               

QY-x164   HeLa宮頸癌細(xì)胞                                                 

QY-x165   A549 [A-549]肺癌細(xì)胞    CCL-185                                        

QY-x166   MCF7乳腺癌細(xì)胞  HTB-22                                         

QY-x167   Hep 3B2.1-7肝癌細(xì)胞    HB-8064                                       

QY-x168   MDA-MB-231乳腺癌細(xì)胞      HTB-26                                         

QY-x169   Hep G2肝癌細(xì)胞    HB-8065