1. 酶標(biāo)儀(450nm 波長濾光片)

2. 高精度移液器，EP 管及一次性吸頭

3. 37℃恒溫箱，雙蒸水或去離子水

4. 吸水紙

【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！【植物總激素(totalhormone)ELISA試劑盒現(xiàn)貨】

ELISA應(yīng)用：

ELISA檢測(cè)試劑盒、ELISAKit、酶聯(lián)免疫試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒、酶聯(lián)免疫分析試劑盒、酶免試劑盒等,比較常見的叫法是ELISA檢測(cè)試劑盒、酶聯(lián)免疫吸附測(cè)定試劑盒等ELISA試劑盒自從60-70年代問世以來,得到全世界科研工作者的認(rèn)可及推崇,在歐美及中國獲得很大的推廣,尤其是國內(nèi)生化領(lǐng)域的長足發(fā)展.Elisa生物試驗(yàn)是一種敏感性高,特異性強(qiáng),重復(fù)性好的實(shí)驗(yàn)診斷方法.由于其試劑穩(wěn)定、易保存,操作簡(jiǎn)便,結(jié)果判斷較客觀等因素,已廣泛應(yīng)用在免疫學(xué)檢驗(yàn)的各領(lǐng)域中.ELISA檢測(cè)試劑盒適用于體外定性檢測(cè)人血清或血漿中的抗人類戊型肝炎（HEV）病毒IgM抗體ELISA檢測(cè)

【植物總激素(totalhormone)ELISA試劑盒現(xiàn)貨】齊一生物銷售：021－6034 8496；181214 53965；173021 04490  web：www.qiyibio.com

試劑盒安全性提示：

1.工作人員實(shí)驗(yàn)中避免直接接觸終止液和底物A、B。一旦接觸到這些液體，請(qǐng)盡快用水沖洗。

2.實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。

3.不要用嘴吸取試劑盒里的任何成份。

樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【植物總激素(totalhormone)ELISA試劑盒現(xiàn)貨】ELISA實(shí)驗(yàn)原理

試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè).先將生物素標(biāo)記的抗體同時(shí)溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.

免疫測(cè)定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測(cè)定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動(dòng)物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測(cè)定試劑幾成為現(xiàn)實(shí)的新一代試劑,各種新型使用的測(cè)定方法也不斷出現(xiàn).

HBsAg試劑特點(diǎn)（檢測(cè)模式：臨床抗體夾心法）：包被抗體為山羊多抗.多抗具有高親和性和對(duì)抗原各種表位的反應(yīng)性.酶表抗體為與HBsAg有不同結(jié)合位點(diǎn)的鼠復(fù)合單位,可測(cè)得HBsAg變異樣品,提高檢測(cè)的特異性（99.98%）提高檢測(cè)的靈敏度,應(yīng)用ParlEhrich學(xué)說（PEI）HBsAg標(biāo)準(zhǔn)品,對(duì)ad標(biāo)準(zhǔn)品的靈敏度為0.05ng/ml對(duì)ay標(biāo)準(zhǔn)品靈敏度為0.025ng/ml

ELISA檢測(cè)試劑盒應(yīng)用定性夾心免疫檢測(cè)技術(shù),用合成的HEV多肽抗原包被微孔板板條,這些多肽是中國型HEV毒株核心氨基酸序列中抗原性很強(qiáng)的肽段,分別來自于該毒株的開放閱讀框2和開放閱讀框3.將樣品或標(biāo)準(zhǔn)品加入孔中并孵育,如果其中存在HEVIgM抗體,這些抗體就會(huì)與HEV的多肽抗原結(jié)合,并固定在上面,洗板除去其它非特異性抗體和樣品中的其它成份.然后加入羊抗人IgM-HRP（辣根過氧化物酶）酶結(jié)合物,經(jīng)第二次孵育后,酶結(jié)合物就會(huì)與次孵育結(jié)合上的HEVIgM抗體相結(jié)合,洗板除去未結(jié)合的酶結(jié)合物,加入TMB底物溶液,在第三次孵育時(shí)會(huì)發(fā)生酶-底物反應(yīng),只有那些含有HEVIgM抗體和酶結(jié)合物所形成的復(fù)合物的孔才會(huì)發(fā)生顏色變化,加入硫酸溶液終止酶和底物間的反應(yīng),并在波長:450nm處測(cè)量O.D.值,按照本HEVIgM抗體elisa試劑盒的測(cè)試標(biāo)準(zhǔn),O.D.值大于或等于Cut-Off值的樣品被認(rèn)為是初試陽性.

以上資料僅供參考了解，不供于論文寫作

【植物總激素(totalhormone)ELISA試劑盒現(xiàn)貨】齊一生物專業(yè)為您提供高品質(zhì)，高靈敏度.高質(zhì)量的酶聯(lián)免疫試劑盒，齊一生物長期經(jīng)營ELISA試劑盒（免費(fèi)代測(cè)）及抗體、分子學(xué)生物試劑盒、金標(biāo)法檢測(cè)試劑盒、比色法檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

QYD604  豬丹毒 - 抗體檢測(cè)ELISA  96孔/盒                

QYD605  豬丹毒 - 抗原檢測(cè)ELISA  96孔/盒                

QYD606  豬傳染性胃腸炎 - 抗體檢測(cè)ELISA  96孔/盒                

QYD607  豬沙門氏菌 - 抗體檢測(cè)ELISA  96孔/盒                

QYD608  豬流感- 抗體檢測(cè)ELISA   96孔/盒                 【植物總激素(totalhormone)ELISA試劑盒現(xiàn)貨】

QYD609  豬流感- 抗原檢測(cè)ELISA   96孔/盒                

QYD610  豬副嗜血桿菌- 抗體檢測(cè)ELISA 96孔/盒                

QYD611  豬輪狀病毒- 抗體檢測(cè)ELISA   96孔/盒                

QYD612  豬輪狀病毒- 抗原檢測(cè)ELISA   96孔/盒                

QYD613  反芻動(dòng)物弓形蟲抗體檢測(cè)ELISA 96孔/盒                

QYD614  非洲豬瘟抗體檢測(cè)試劑盒  96孔/盒                

QYD615  鯉魚卵黃蛋白原試劑盒    96孔/盒                

QYD616  虹鱒魚卵黃蛋白原試劑盒  96孔/盒                

QYD617  鮭科魚卵黃蛋白原試劑盒  96孔/盒                

QYD618  斑馬魚卵黃蛋白原試劑盒  96孔/盒                

QYD619  青鳉魚卵黃蛋白原試劑盒  96孔/盒                

QYD620  黑魚卵黃蛋白原試劑盒    96孔/盒                

QYD621  麻痹性貝類毒素（PSP）試劑盒 96孔/盒                

QYD622  腹瀉性貝類毒素（DSP）試劑盒 96孔/盒                

QYD623  健忘性貝類毒素（ASP）試劑盒 96孔/盒                

QYD624  組胺試劑盒  96孔/盒                 【植物總激素(totalhormone)ELISA試劑盒現(xiàn)貨】

QYD625  葡萄糖試劑盒    96孔/盒                

QYD626  果糖葡萄糖試劑盒    96孔/盒                

QYD627  氨氮試劑盒  96孔/盒                

QYD628  硝酸鹽亞硝酸鹽試劑盒    96孔/盒                

QYD629  維生素C試劑盒  96孔/盒                

QYD630  尿素試劑盒  96孔/盒                

QYD631  果糖試劑盒  96孔/盒                

QYD632  皮革水解蛋白試劑盒  96孔/盒                

QYD633  物種鑒定（孔測(cè))試劑盒   96孔/盒                

9011965 Tip Adapter 300 µl BR8000       CNY            

204554  QuantiFast Probe RT-PCR+ROXvial Kit(400)        CNY            

133064  NeXtalStock Magnesium sulfate 6H2O (200)        CNY            

870101  EGFR RGQ PCR Kit (24)       CNY            

290023  mericon L. monocytogenes Kit (24)       CNY            

204443  QuantiTect Probe RT-PCR Kit (200)   For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect Probe RT-PCR Master Mix,* 100 ul QuantiTect RT Mix, 2 x 2.0 ml RNase-free water CNY            

133052  NeXtalStock CTAB (200)      CNY            

9018397 Transport Box, QIAxcel      CNY             【植物總激素(totalhormone)ELISA試劑盒現(xiàn)貨】

9001650 Rotor-Gene Q 5plex HRM System       CNY            

37612   Qproteome Mitochondria Isolation Kit        CNY            

9015333 O-rings, pressure sensor fixing, M48 (2)        CNY            

133032  NeXtalStock Citric acid  (200)      CNY            

133023  NeXtalStock Cadmium chloride  (200)     CNY            

9001638 Rotor-Gene Q 2plex HRM (w/o PC) Priority    Rotor-Gene Q 2plex HRM (w/o PC) Priority includes the Rotor-Gene Q system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service has response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents.   CNY            

9001611 QIAgility HEPA / UV (incl.Laptop)       CNY            

9001601 Rotor-Disc 72 Starter Kit (230V)        CNY            

239901  GelPilot DNA Loading Dye,5x (6 x 500µl)     CNY            

9015318 Sensor, photo, EE-SX674A        CNY            

133142  NeXtalStock Lithium sulfate (200)       CNY            

1036685 HiPerFect Transfection Reagent (50 ml)      CNY            

9242791 RCS, PM Agreement   The Preventive Maintenance (PM) service ensures the correct operation of the equipment. During the PM, all the instrument modules are inspected by a qualified QIAGEN service technician. The Preventive Maintenance is performed under the manufacturer guidelines ａnd documented according to GLP / GMP CNY            

37101   PhosphoProtein Purification Kit (6) 6 PhosphoProtein Purification Columns, 6 Nanosep Ultrafiltration Columns, Reagents, Buffers  CNY            

950211  Liquid Digest Reagent (VXL) (60 ml)     CNY            

950111  Liquid Digest Reagent (DXL) (60 ml)     CNY             【植物總激素(totalhormone)ELISA試劑盒現(xiàn)貨】

9015331 Tube, silicon, M48 (6)      CNY            

133131  NeXtalStock Zinc sulfate  (200)     CNY            

133128  NeXtalStock Sodium citrate pH 5.6 (200)     CNY            

133009  NeXtalStock ADA pH 6.5 (200)        CNY            

9011942 Thermostate system, 220V , V1.03        CNY            

9011941 Labware tracking system, hand-held      CNY            

37009   Qproteome Albumin/IgG Depletion Plate       CNY            

QYD570  大腸桿菌腸毒素(VT1、VT2)免疫膠體金試劑盒      96孔/盒                             

QYD571  蠟樣芽胞桿菌毒素免疫膠體金試劑盒       96孔/盒                             

QYD572  軍團(tuán)菌免疫膠體金試劑盒    96孔/盒                             

QYD573  新城疫ELISA試劑盒（NDV-Ab)       96孔/盒                             

54362     QIAamp cador Pathogen QXT Plus Kit (10)        CNY                           

9012680  Peristaltic Pump, 1 channel (A04897)          CNY                           

9012666  Pipetting system upgrade 2, BR3000            CNY                           

970480    EGFR Pyro Kit (24)              CNY                           

133204    NeXtalStock Jeffamine ED 2001 pH 7 (200)              CNY                           

929204    QIAxcel Protein Kit (1200)           CNY                            【植物生長素（Auxin）ELISA試劑盒現(xiàn)貨】

929004    QIAxcel DNA Screening Kit (2400)             CNY                           

9012624  Pippeting Station, 2 microtitre plates            CNY                           

206145    QIAGEN Multiplex PCR Kit (1000)      "contains

1 x Multiplex PCR MasterMix 2x, (1 x 25 ml)

(Mastermix contains HotStarTaq® DNA Polymerase, Multiplex PCR Buffer*, dNTP Mix)

1 x Q-Solution (10ml)

1 x RNase-free water (20 ml)

Handbook"      CNY                           

205311    QuantiTect Rev.Transcription Kit (50)  "For 50 x 20 µl reactions: gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, RNase-Free Water.

Suitable only for cDNA synthesis followed by real-time analyses."     CNY                           

205211    Sensiscript RT Kit (50)  For 50 reverse-transcription reactions: Sensiscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water CNY                           

291514    mericon Quant RoundUp Ready Soy (48)            CNY                           

9001913  PyroMark Q24, Priority PyroMark Q24, Priority includes the PyroMark Q24 system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. (In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs).       CNY                           

133114    NeXtalStock Sodium nitrate (200)        CNY                           

965752    QIAamp Media MDx Kit (12)       "Reagent Rental" -> price not published on the web or in the catalog  CNY                           

133189    NeXtalStock Copper sulfate (200)        CNY                           

133182    NeXtalStock Citric acid pH 3.4 (200)          CNY                           

9018473  Transport box, QIAC            CNY                           

133097    NeXtalStock Potassium fluoride (200)         CNY                           

133094    NeXtalStock Potassium bromide (200)        CNY                           

9001878  QIAxcel, Priority           CNY                           

9001872  EZ1 Advanced, Priority  EZ1 Advanced, Priority includes the EZ1 Advanced instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                            【植物生長素（Auxin）ELISA試劑盒現(xiàn)貨】

958034    EZ1 RNA Cell Mini Kit (48)   "general purpose device,

48 Reagent Cartridges (RNA Cell) 100 Disposable Tip Holders, 100 Disposable Filter-Tips, 50 Sample Tubes (2 ml), 50 Elution Tubes (1.5 ml), Buffer RLT, RNase-Free DNase I" CNY                           

955336    MagAttract Virus Mini M48 Kit (192)          CNY                           

952034    EZ1 DNA Investigator Kit (48)             CNY                           

9018450  Piston, detect (1), QX           CNY                           

9015410  O-rings, nozzle, M96 (8)              CNY                           

9012423  Unit, gripper T (including grippers) P3         CNY                           

204972    Rotor-Gene Multiplex RT-PCR Kit (80)        CNY                           

204956    QuantiFast Multiplex RT-PCR+R Kit (2000)        CNY                           

133170    NeXtalStock CAPSO pH 9.6 (200)              CNY