【友情提示】：本產(chǎn)品僅供科研研究使用，不得用于人體臨床直接檢測(cè)。避免給您帶來不必要的損失，請(qǐng)仔細(xì)閱讀購買說明！

本試劑盒僅供研究使用，齊一生物銷售：021－6034 8496；181214 53965；173021 04490歡迎選購！

操作步驟

1.準(zhǔn)備試劑，樣品和標(biāo)準(zhǔn)品

2.加入準(zhǔn)備好的樣品和標(biāo)準(zhǔn)品，37℃反應(yīng)30分鐘

3.洗板5次，加入酶標(biāo)試劑，37℃反應(yīng)30分鐘

4.洗板5次，加入顯色液A、B，37℃顯色15分鐘

5.加入終止液

6.15分鐘之內(nèi)讀OD值

7.計(jì)算
齊一生物供應(yīng)ELISA試劑盒檢測(cè)的目的是為實(shí)驗(yàn)提供準(zhǔn)確可靠的定量分析實(shí)驗(yàn)依據(jù)。為了保證實(shí)驗(yàn)數(shù)據(jù)的可靠性，在實(shí)驗(yàn)過程中必須堅(jiān)持全面質(zhì)量控制和全過程質(zhì)量控制。在收集標(biāo)本前都必須有一個(gè)完整的計(jì)劃。

【植物生長(zhǎng)素（Auxin）ELISA試劑盒現(xiàn)貨】樣本保存條件：

1.2-8℃冰箱*多放2周

2.-20到-50℃的冰箱可以放3-6個(gè)月

3.-50℃以下的冰箱，可以放一年

【植物生長(zhǎng)素（Auxin）ELISA試劑盒現(xiàn)貨】樣本收集

1.血清樣本用干燥管（紅色）或者促凝管（黃色）收集

2.血漿用抗凝管，一般有EDTA抗凝（紫色）、枸櫞酸鈉抗凝（黑色或者藍(lán)色）

3.動(dòng)物組織勻漿（細(xì)胞）：

              1g組織加入9ml PBS（濃度為0.1M，pH為7.4的）測(cè)分泌型指標(biāo)

              1g組織加入1ml組織裂解液加入8ml PBS 測(cè)細(xì)胞內(nèi)

糞便樣本：1g樣本+9ml PBS（濃度為0.01M，pH為7.4的）

沒有PBS的話也可以用一下生理鹽水，還是用PBS

【植物生長(zhǎng)素（Auxin）ELISA試劑盒現(xiàn)貨】樣本離心轉(zhuǎn)速及時(shí)間

1.試管采集的樣本：3000轉(zhuǎn)，離心五分鐘取上清

2.離心管或者EP管采集的樣本：5000－－8000轉(zhuǎn)3分鐘取上清

3..樣本保存－－如果樣品不立即使用，應(yīng)將其分成小部分-70 ℃保存，避免反復(fù)冷凍。盡可能的不要使用溶血或高血脂血。如果血清中大量顆粒，檢測(cè)前先離心或過濾。不要在37℃或更高的溫度加熱解凍。應(yīng)在室溫下解凍并確保樣品均勻地充分解凍。

【植物生長(zhǎng)素（Auxin）ELISA試劑盒現(xiàn)貨】以上樣本采集及處理方式供您參考，如有問題請(qǐng)來電，齊一生物銷售：021－6034 8496；181214 53965；173021 04490

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QYD552  沙門氏菌檢測(cè)試劑盒    96孔/盒                             

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QYD558  大腸桿菌/大腸菌群定量計(jì)數(shù)板   96孔/盒                             

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QYD565  沙門氏菌免疫膠體金試劑盒       96孔/盒                             

QYD566  大腸桿菌O157免疫膠體金試劑盒     96孔/盒                             

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QYD573  新城疫ELISA試劑盒（NDV-Ab)       96孔/盒                             

54362     QIAamp cador Pathogen QXT Plus Kit (10)        CNY                           

9012680  Peristaltic Pump, 1 channel (A04897)          CNY                           

9012666  Pipetting system upgrade 2, BR3000            CNY                           

970480    EGFR Pyro Kit (24)              CNY                           

133204    NeXtalStock Jeffamine ED 2001 pH 7 (200)              CNY                           

929204    QIAxcel Protein Kit (1200)           CNY                            【植物生長(zhǎng)素（Auxin）ELISA試劑盒現(xiàn)貨】

929004    QIAxcel DNA Screening Kit (2400)             CNY                           

9012624  Pippeting Station, 2 microtitre plates            CNY                           

206145    QIAGEN Multiplex PCR Kit (1000)      "contains

1 x Multiplex PCR MasterMix 2x, (1 x 25 ml)

(Mastermix contains HotStarTaq® DNA Polymerase, Multiplex PCR Buffer*, dNTP Mix)

1 x Q-Solution (10ml)

1 x RNase-free water (20 ml)

Handbook"      CNY                           

205311    QuantiTect Rev.Transcription Kit (50)  "For 50 x 20 µl reactions: gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, RNase-Free Water.

Suitable only for cDNA synthesis followed by real-time analyses."     CNY                           

205211    Sensiscript RT Kit (50)  For 50 reverse-transcription reactions: Sensiscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water CNY                           

291514    mericon Quant RoundUp Ready Soy (48)            CNY                           

9001913  PyroMark Q24, Priority PyroMark Q24, Priority includes the PyroMark Q24 system, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service ａnd response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor ａnd travel costs with installation performance ａnd service visits documented on a GMP/GLP-compliant Field Service documents. (In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs).       CNY                           

133114    NeXtalStock Sodium nitrate (200)        CNY                           

965752    QIAamp Media MDx Kit (12)       "Reagent Rental" -> price not published on the web or in the catalog  CNY                           

133189    NeXtalStock Copper sulfate (200)        CNY                           

133182    NeXtalStock Citric acid pH 3.4 (200)          CNY                           

9018473  Transport box, QIAC            CNY                           

133097    NeXtalStock Potassium fluoride (200)         CNY                           

133094    NeXtalStock Potassium bromide (200)        CNY                           

9001878  QIAxcel, Priority           CNY                           

9001872  EZ1 Advanced, Priority  EZ1 Advanced, Priority includes the EZ1 Advanced instrument, installation ａnd extended warranty. Installation covers hardware ａnd software installation of system ａnd provides user with introductory training. Priority package extends warranty period of the system to 2 years. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Service package includes all parts, labor ａnd travel costs with a service response time of 48 hours (2 working days). Performance of the installation ａnd service visits are documented on a GMP/GLP-compliant Field Service documents.       CNY                            【植物生長(zhǎng)素（Auxin）ELISA試劑盒現(xiàn)貨】

958034    EZ1 RNA Cell Mini Kit (48)   "general purpose device,

48 Reagent Cartridges (RNA Cell) 100 Disposable Tip Holders, 100 Disposable Filter-Tips, 50 Sample Tubes (2 ml), 50 Elution Tubes (1.5 ml), Buffer RLT, RNase-Free DNase I" CNY                           

955336    MagAttract Virus Mini M48 Kit (192)          CNY                           

952034    EZ1 DNA Investigator Kit (48)             CNY                           

9018450  Piston, detect (1), QX           CNY                           

9015410  O-rings, nozzle, M96 (8)              CNY                           

9012423  Unit, gripper T (including grippers) P3         CNY                           

204972    Rotor-Gene Multiplex RT-PCR Kit (80)        CNY                           

204956    QuantiFast Multiplex RT-PCR+R Kit (2000)        CNY                           

133170    NeXtalStock CAPSO pH 9.6 (200)              CNY