【虹膜色素上皮細(xì)胞總DNA】下面是細(xì)胞株培養(yǎng)的具體無(wú)菌技術(shù)要點(diǎn)：

1. 實(shí)驗(yàn)開始前，無(wú)菌室和無(wú)菌操作臺(tái)需要紫外光照射30到60分鐘完全滅菌，用70%ethanol擦拭無(wú)菌操作臺(tái)面，并且打開無(wú)菌操作臺(tái)風(fēng)扇運(yùn)行10分鐘之后，才可以進(jìn)行實(shí)驗(yàn)操作。每次操作只能處理一株細(xì)胞株，即使培養(yǎng)基完全相同也不可共用培養(yǎng)基，以避免細(xì)胞間污染或失誤混淆。實(shí)驗(yàn)完成后，請(qǐng)將實(shí)驗(yàn)物品拿出工作臺(tái)，用70%ethanol再次擦拭無(wú)菌操作臺(tái)面。操作間隔應(yīng)該讓無(wú)菌操作臺(tái)運(yùn)轉(zhuǎn)10分鐘到15分鐘后，才能進(jìn)行下一個(gè)細(xì)胞株的操作。

2. 無(wú)菌操作工作的區(qū)域應(yīng)保持清潔和寬敞，必要物品（試管架、吸管、吸取器或吸管盒等）可以暫時(shí)放置，其它實(shí)驗(yàn)用品使用完畢后應(yīng)移出，有利于空氣流通。實(shí)驗(yàn)用品需70%ethanol擦拭后方可帶入無(wú)菌操作臺(tái)內(nèi)。實(shí)驗(yàn)操作過(guò)程應(yīng)在臺(tái)面的中央無(wú)菌區(qū)域內(nèi)完成，請(qǐng)勿在邊緣非無(wú)菌區(qū)域進(jìn)行操作。

3. 小心取用無(wú)菌實(shí)驗(yàn)物品，避免細(xì)菌污染。請(qǐng)勿觸碰吸管尖頭部和容器瓶口處，也不要在打開的容器正上方進(jìn)行操作。容器打開后，請(qǐng)用手夾住瓶蓋并輕握瓶身，傾斜大約45°角取用，盡量不要將瓶蓋口朝上放置于桌面。

4. 工作人員應(yīng)當(dāng)首先注意自身安全，必須穿戴齊實(shí)驗(yàn)衣和實(shí)驗(yàn)手套后才能進(jìn)行實(shí)驗(yàn)。對(duì)于病毒感染或是來(lái)自人類的細(xì)胞株應(yīng)當(dāng)特別小心操作，并選擇適當(dāng)?shù)燃?jí)的無(wú)菌操作臺(tái)進(jìn)行（至少是Class II）。操作過(guò)程中，應(yīng)避免引起aerosol的產(chǎn)生，小心有毒性藥品，例如DMSO和TPA等，并避免針頭的傷害等等。

5. 定期檢測(cè)下列項(xiàng)目：

5.1. CO2鋼瓶的CO2壓力

5.2. CO2培養(yǎng)箱的CO2濃度、溫度、和水盤是否有污染（水盤的水需用無(wú)菌水，每周定時(shí)更換）。

5.3. 無(wú)菌操作臺(tái)內(nèi)的airflow壓力，定期更換紫外線燈管和HEPA過(guò)濾膜，預(yù)濾網(wǎng)（300小時(shí)/預(yù)濾網(wǎng)，3000小時(shí)/HEPA）。

6. 水槽內(nèi)可添加消毒劑（Zephrin 1:750），需定期更換水槽中的水。

【虹膜色素上皮細(xì)胞總DNA】齊一生物友情提醒，本產(chǎn)品僅供科研使用，不得用于臨床及診斷使用！

一細(xì)胞說(shuō)明，具體詳細(xì)請(qǐng)見(jiàn)細(xì)胞附帶說(shuō)明書

 二、客戶自備試劑

1、PBS  

2、 2、RPMI-1640 培養(yǎng)基（不含 Hepes）+ 10%胎牛血清+ 1%雙抗

3、0.25%  （W/V）胰蛋白酶（含 0.02% EDTA）

三、細(xì)胞背景

1、生長(zhǎng)方式：貼壁

2、種屬：Homo sapiens  人

四、培養(yǎng)條件

1、培養(yǎng)基：RPMI-1640 培養(yǎng)基（不含 Hepes）+ 10%胎牛血清+ 1%雙抗

2、溫度：37.0°C

3、氣體:空氣  95%，CO2 5%

【虹膜色素上皮細(xì)胞總DNA】五、培養(yǎng)方法

 收到細(xì)胞后，在倒置顯微鏡下觀察整個(gè)細(xì)胞生長(zhǎng)情況：

 如果細(xì)胞未長(zhǎng)滿，用 75%酒精噴灑整個(gè)瓶消毒后放到超菌臺(tái)內(nèi)，嚴(yán)格無(wú)菌操作，打開細(xì)胞培養(yǎng)瓶，留10ml 培養(yǎng)液繼續(xù)培養(yǎng)。如果細(xì)胞已長(zhǎng)滿（達(dá) 80-90%）。即可進(jìn)行傳代，具六、體步驟如下：

1，棄去培養(yǎng)液，用PBS洗 1-2次。

2，向瓶?jī)?nèi)加入 1.0-2.0ml 胰蛋白酶液，在倒置顯微鏡下觀察細(xì)胞消化情況，若細(xì)胞大部分變圓，迅速拿回操作臺(tái)，吸取胰蛋白酶，加含有 6ml  含 10%血清的培養(yǎng)液，輕輕吹打細(xì)胞。

3，加入等量的的培養(yǎng)液，輕輕吹打混勻后吸出一半，分到新的培養(yǎng)

4，傳代比例：1:2-1:3  

七、常見(jiàn)問(wèn)題及解決方案

1、培養(yǎng)瓶有破裂，培養(yǎng)液有漏液：細(xì)胞極大可能會(huì)污染，所以我們會(huì)及時(shí)安排幫老師解決。

2、細(xì)胞漂�。号囵B(yǎng)瓶不開封，瓶口酒精擦拭后平躺放置在培養(yǎng)箱。次日觀察，如細(xì)胞大部分又貼回瓶底，表明細(xì)胞活力正常，剩余漂浮的細(xì)胞可以去掉，留 10ml 培養(yǎng)液培養(yǎng)觀察，細(xì)胞生長(zhǎng)至匯合度 80%，進(jìn)行消化傳代；如細(xì)胞還是不貼壁，將細(xì)胞離心收集轉(zhuǎn)到新培養(yǎng)瓶，原培養(yǎng)瓶加部分培養(yǎng)液繼續(xù)培養(yǎng)，中間注意觀察，我們的技術(shù)人員會(huì)一直跟蹤指導(dǎo)，直到問(wèn)題解決。

細(xì)胞培養(yǎng)代理品牌

細(xì)胞培養(yǎng)代理品牌

【虹膜色素上皮細(xì)胞總DNA】齊一生物科技（上海）有限公司是一家專業(yè)生產(chǎn)和銷售各種生化試劑,細(xì)胞株、原代細(xì)胞、菌種、醫(yī)藥中間體,標(biāo)準(zhǔn)品和對(duì)照品的大型化學(xué)科技公司. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490；Web:www.qiyibio.com

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9236264  Elution Microtubes Adaptor, inject mold              CNY                                  

9236165  O-ring, lubrication kit, RapidPlate         CNY                                  

9235931  Splashing guard, BR8 RD             CNY                                  

9230822  Rack for reagents,Trough (for 6 troughs)           CNY                                  

9235707  Washer Cable, RCS1             CNY                                  

9235695  Ground Wire, RCS1              CNY                                  

9235332  Z-Rack Packing Cover          CNY                                  

9235118  Fuse F1A, v1.00           CNY                                  

9235114  Fuse FST4A, v1.00              CNY                                  

9230054  Clamp, Tubing, Z-bar           CNY                                  

9234861  Lid coupling piece for system liquid             CNY                                  

9239551  Cbl Assy, XY, LED Driver            CNY                                  

9238663  Hook, motorized lid lock              CNY                                  

9238642  Capacitor board             CNY                                  

9238626  Opto-Encoder        CNY                            【虹膜色素上皮細(xì)胞總DNA】  

9238625  Eprom ａnd controller 4K15, Zent3C            CNY                                  

9241828  Pyro Q96 MDa, Premium Agreement    "Repair service delivery: onsite

Service response time (repair): 24 hours/1 business day

Periodic inspection/maintenance: yes, 1/agreement year

Inspection/maintenance delivery: onsite

Cost coverage for Repair Parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: not included

Transportation cost coverage: not applicable"      CNY                                  

9241122  Superlube              CNY                                  

9241007  QIArent&go - Rent out fee QIAcube           CNY                                  

9240985  PCB, drive, cooling device, Qsym        CNY                                  

9240982  Sensor, x, sample tracking, Qsym        CNY                                  

9240951  Support, cable, portal, Qsym        CNY                                  

9234820  Manual, user, QS 4.0, Int             CNY                                  

9240768  Signal Conditioner, Centrifuge, AP96           CNY                                  

9240764  DC Output Module 8NPT, 3AMP, AP96              CNY                                  

6000-5003      Duraseal Sealing Film            CNY                                  

9241781  RGQ, Full Agreement, no PM       "Repair service delivery: send to Regional Repair Center

Service response time (repair): 7-10 days turnaround time

Periodic inspection/maintenance: not included

Inspection/maintenance delivery: not applicable

Cost coverage for repair parts: yes, included

Cost coverage for labor: yes, included

Cost coverage for travel: yes, included

Replacement system (Loaner) provision: yes, included (provision time 2 days)

Transportation cost coverage: yes, included"       CNY                                  

9241776  QIAgility, Inspection Service "The Annual Inspection Service, QIAgility is an on-site equipment service, performed by a QIAGEN service specialist.

All required testing material ａnd certified equipment is provided by the service specialist.

During the half day-long procedure, all application-critical modules of the QIAgility are inspected ａnd tested ａnd a pipetting head calibration is conducted. Test results are documented in a GMP/GLP-compliant Visit Report." CNY                                  

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9236426  Board, arm interconnect, TWI             CNY                                  

QY-x1172       人乳腺導(dǎo)管癌細(xì)胞MDA-MB-453                                         

QY-x1173       人乳腺細(xì)胞HBL-100                                      

QY-x1174       人乳腺癌細(xì)胞BCaP-37                                   

QY-x1175       人乳腺癌細(xì)胞LCC1                                       

QY-x1176       人乳腺癌細(xì)胞MX-1                                       

QY-x1177       人乳腺管癌細(xì)胞T47D                                    

QY-x1178       人乳腺癌細(xì)胞SK-BR-3                                    【虹膜色素上皮細(xì)胞總DNA】

QY-x1179       人乳腺癌細(xì)胞4T1                                   

QY-x1180       人乳腺癌細(xì)胞HCC1937                                         

QY-x1181       人乳腺導(dǎo)管癌細(xì)胞HCC38                                     

QY-x1182       人乳腺癌細(xì)胞Hs-578T                                   

QY-x1183       人乳腺癌Tamoxifen耐藥株LCC2                                        

QY-x1184       人乳腺癌抗雌激素耐藥株LCC9                                    

QY-x1185       人乳腺癌阿霉素耐藥細(xì)胞株MCF/Adr                                         

QY-x1186       人乳腺癌細(xì)胞BT-549                                     

QY-x1187       人乳腺導(dǎo)管瘤細(xì)胞BT-474                                     

QY-x1188       人乳腺癌細(xì)胞MB-271                                    

QY-x1189       人乳腺癌細(xì)胞ER-30                                      

QY-x1190       人乳腺癌細(xì)胞M453                                       

QY-x1191       人乳腺浸潤(rùn)性導(dǎo)管癌旁皮膚細(xì)胞CCD-1095Sk                                   

QY-x1192       人乳腺細(xì)胞HBL-100                                      

QY-x1193       人乳腺導(dǎo)管瘤細(xì)胞UACC812                                        

QY-x1194       人乳腺癌細(xì)胞系1590                                      【虹膜色素上皮細(xì)胞總DNA】

QY-x1195       人卵巢癌細(xì)胞A2780                                      

QY-x1196       人卵巢癌細(xì)胞Sw626                                     

QY-x1197       人卵巢癌順鉑耐藥株COC1/CDDP                                       

QY-x1198       人卵巢癌紫杉醇耐藥株A2780/Taxol                                    

QY-x1199       人卵巢癌細(xì)胞系Anglne                                         

QY-x1200       人卵巢癌細(xì)胞OVCaR-3                                         

QY-x1201       人卵巢癌細(xì)胞Caov-3                                     

QY-x1202       人卵巢癌細(xì)胞HO-8910