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膀胱成纖維細胞總DNA

點擊次數(shù):16發(fā)布時間:2016/6/29 20:48:14

膀胱成纖維細胞總DNA

更新日期:2016/6/29 20:48:14

所 在 地:中國大陸

產(chǎn)品型號:

簡單介紹:膀胱成纖維細胞總DNA 1μg/1μl(10μg/20ug/50ug) 齊一生物科技(上海)有限公司銷售:021-6034 8496;181214 53965;173021 04490

相關(guān)標簽:膀胱成纖維細胞總DNA 

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 膀胱成纖維細胞總DNA齊一生物提供多國品牌細胞產(chǎn)品,公司常年備用細胞現(xiàn)貨,能滿足全國客戶對細胞研究的需求。齊一生物銷售:0216034 8496;181214 53965;173021 04490

選擇齊一生物的原因:

1.供貨及時。我們訂貨周期短,運輸時間短,在*短時間內(nèi)為您提供所需產(chǎn)品。

2.價格優(yōu)惠:美國ATCC的中國代理商,沒有中間環(huán)節(jié)。在國內(nèi)我們保證價格。

3.服務(wù)保障:我們擁有專用的技術(shù)指導(dǎo)團隊,對每售出的一件產(chǎn)品,提供全面的售前、售中和售后服務(wù)。

4.產(chǎn)品種類齊全:25個系統(tǒng)共150多種細胞以及各類菌株,滿足不同客戶的要求。細胞經(jīng)過嚴格的質(zhì)控,純度可達98%。常用產(chǎn)品備有現(xiàn)貨。其產(chǎn)品類型如下:消化系統(tǒng)細胞株,呼吸系統(tǒng)細胞株,泌尿系統(tǒng)細胞株生殖系統(tǒng)細胞株 血液循環(huán)系統(tǒng) 內(nèi)皮細胞細胞株 神經(jīng)系統(tǒng)及其他細胞株,動物細胞株和菌株

5.對于部分難培養(yǎng)的細胞我公司可代復(fù)蘇細胞,并提供技術(shù)支持。

6.經(jīng)驗豐富:我公司是國內(nèi)專業(yè)化的細胞銷售公司,具有十余年細胞產(chǎn)品銷售經(jīng)驗,有專業(yè)的銷售團隊和技術(shù)人員。齊一生物公司的每一位員工都將竭誠為您服務(wù)。

7.質(zhì)量保證,保證售后

8. 齊一生物銷售:0216034 8496;181214 53965;173021 04490

膀胱成纖維細胞總DNA 齊一生物公司專業(yè)代理ScienCell簡介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research and development of cell and cell-related products for experimental and therapeutic use. The ScienCell technology provides a variety of high quality normal human and animal cells, cell culture media and reagents, medium supplements, cell-derived RNA, cDNA and proteins for the research community. Scientists at ScienCell are also studying and developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell's ability to combat human degenerative disorders. We are based in San Diego, California and were established in 1999.

膀胱成纖維細胞總DNA美國ATCC簡介:美國菌種保藏中心, 又稱美國模式菌種收集中心(ATCC),是位于馬里蘭洲洛克菲勒的一家私營的,非贏利性組織。目前它可以提供各種動植物細胞、標準品、菌株達兩萬多種。ATCC成立于1925年,是世界上的生物資源中心,由美國14家生化、醫(yī)學(xué)類行業(yè)協(xié)會組成的理事會負責(zé)管理,是一家全球性、非盈利生物標準品資源中心。ATCC向全球發(fā)布其獲取、鑒定、保存及開發(fā)的生物標準品,推動科學(xué)研究的驗證、應(yīng)用及進步 ATCC可以提供以下類別生物標準品:細胞株(3000多種);菌株(15000多種);動植物病毒株(2500多種)以及重組物質(zhì)等。

 

現(xiàn)已成為可信賴的活體微生物、細胞系等獲得、保存和發(fā)放的國家資源中心。ATCC29,000多種不同品系可靠的動物細胞和微生物培養(yǎng)體,它能滿足各科學(xué)團體對可信賴品系的需要。僅1981年就有39,000多培養(yǎng)物發(fā)放給全世界的科學(xué)工作者,并成功地用冷凍和冷凍干燥的方法保存各種細胞品系。 ATCC是一個非(學(xué)會單位)無利潤的公司,由各科學(xué)組織推選成員組成理事會進行管理,F(xiàn)有工作人員125人。參加的科學(xué)團體有:美國免疫協(xié)會、美國生物化學(xué)會、美國細胞生物學(xué)會、美國微生物學(xué)會、美國動物學(xué)會、美國遺傳學(xué)會、原生動物學(xué)會和組織培養(yǎng)協(xié)會等16個學(xué)術(shù)組織。在ATCC可利用各種材料以及工具和設(shè)備進行廣泛的研究。主要研究項目有:酶聯(lián)免疫吸附分析技術(shù)(ELISA),真核細胞轉(zhuǎn)錄的調(diào)控,雜交瘤、淋巴類和髓類以及其他細胞系的建立,人突變細胞系的基因蛋白質(zhì),借助計算機進行鑒別分析,培養(yǎng)物的特征鑒別和保存技術(shù)的改進,關(guān)于化學(xué)致癌、致畸和致突變的。

膀胱成纖維細胞總DNA齊一生物提醒接收到細胞注意事項

1. 接收到細胞,肉眼觀察細胞培養(yǎng)基顏色,顯微鏡觀察細胞生長情況,并對細胞進行不同倍數(shù)拍照(建議收到時的培養(yǎng)瓶拍一張照片,顯微鏡拍收到時的細胞100X,200X各一張)。

2.75%的酒精消毒(建議配置75%的酒精的水是滅菌過的)。

3.嚴格無菌操作,打開細胞培養(yǎng)瓶。

4.將細胞培養(yǎng)瓶內(nèi)的培養(yǎng)基用吸管完全吸出(嚴禁直接傾倒),放入另一無菌容器中(建議換新的培養(yǎng)基培養(yǎng)細胞)。

5.PBS 2-3ml/次輕輕洗細胞表面,洗3-4次遍,加入0.05%胰酶-EDTA1-1.5ml,顯微鏡下觀察細胞變圓,輕輕拍打培養(yǎng)瓶直到細胞脫落,加入終止液終止。

6.1000rpm,5min離心,重懸細胞。

7.換新的細胞培養(yǎng)瓶,37℃,5%CO2培養(yǎng)。膀胱成纖維細胞總DNA

齊一生物銷售:0216034 8496;181214 53965;173021 04490

 

9240701O-Ring, Shaker, Lower Body, AP96/196CNY

9240696Fitting, Barbed, 1/4" ID tube, APCNY

9243651Comprehen Valid., QIAgility"""The Comprehensive Validation Support plan specific to a laboratory's needs will incorporate all of the requirements for the internal validation guidelines from the FBI's Quality Assurance Standards (QAS).


The Comprehensive Validation Support service includes all travel and labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, and amplify the validation study samples and may require multiple individuals. Usually, two weeks is required to analyze the data and one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.


Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform and complete all extractions, quantifications, amplfications, and capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation. 


Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry and accessories are not included and should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) and required substrates (swabs, materials, etc).   For planning purposes and to ensure a smooth process, the Customer is expected to have all materials on-hand and inventoried one (1) week prior to the validation start date.


Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via e-mail) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) and STR (amp) analysis and linkage to starting samples.


Validation data CD/flashdrive – QIAGEN will review the validation data and draft a validation summary document, ensuring compliance with the FBI's QAS requirements/standards. QIAGEN will send validation data in an electronic format (email/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review and signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.


Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation and generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager and the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """CNY

218076miScript SYBR Green PCR Kit (2000)CNY

271906cattletype Milk Prep Kit (50)CNY

289980intype IC-DNA (1ml)CNY

280437virotype BTV RT-PCR Kit (480)CNY

932836#QIAsymphony Ni-NTA Denat. Kit (384)CNY

800511Verification Sequencing / DNA productionCNY

9238243ZLV BioRobot M96 User ManualCNY

9019988ZLV Mainboard, QGYCNY

9020553Foam angle, transport box, QIACCNY

9020582X-Motor, DML3000CNY

9020584Y-Belt, DML3000 320 TeethCNY

9020594Enclosure, DML3000CNY

9021006Motor, Pipettor Y, DCUCNY

9236333Sample LoopCNY

9013382Shaker adapter, microplate, LHSCNY

9013275Motor, gripper, handler T/L, BR3000CNY

9013269Fitting, PA tubing M6x1, 2.25mm, (10)CNY

9013210Cabling kit, moving, BR8000CNY

9013198Tubing,pres.sen.con.auto vac,BR8000v1.00CNY

9013188Valve set, pressure relaese, dispenser,CNY

9011730Cable, level sensor, BR8000CNY

9011686Microplate station, shortStation for accommodating a 96-well plate (or other labware with the same base dimensions) on the worktableCNY

132629NeXtal Stock Sodium citrate untit. (50)1M tri-Sodium citrate dihydrate - Untitrated - 50mL (4 x 12,5 ml tube)CNY

800300Project ConsultingCNY

800231Publication-Ready Sequencing HT, w/ prepCNY


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QY-19372Anti-VTG(vitellogenin)卵黃蛋白原抗體
QY-19382Anti-VWF (Von Willebrand Factor)血管假性血友病因子/血管性血友病因子抗體
QY-19392Anti-WNK4(Ser/Thr-protein kinase WNK4; protein kinase with no lysine 4; protein kinase, lysine –dficient 4)一種新的絲氨酸/蘇氨酸激酶家族成員的基因WNK4抗體
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QY-19422anti-XIAP(X-linked Inhibitor of Apoptosis Protein)X-連鎖凋亡蛋白/性連鎖凋亡抑制蛋白抗體
QY-19432anti-XAF1(XIAP-associated factor 1)XIAP相關(guān)因子1抗體
QY-19442Anti-XRCC1(X-ray Repair Cross Complementing 1)X射線修復(fù)交叉互補蛋白/細胞堿基切除修復(fù)基因抗體
QY-19452Anti-ZCWCC1(Zinc finger CW-type coiled-coil domain protein 1)ZCWCC1抗體
QY-19462Anti-Z-DEVD-FMK(CASPASE3/CPP32 INHIBITOR W-3)CASPASE3凋亡抑制劑
QY-19472Anti-ZNF268 (zinc finger protein ZNF268)- middle (1b)鋅指脂蛋白-1b抗體
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QY-19502Anti-ZNF300 (zinc finger protein ZNF300)- middle鋅指蛋白300抗體
QY-19512anti-ZO-1(zonula occludens-1)胞質(zhì)緊密粘連蛋白1抗體
QY-19522Anti-β-Actin/HRP辣根過氧化物酶標記β-肌動蛋白(抗體)
QY-19532Anti-人T亞群/NK細胞檢測試劑盒人T淋巴細胞亞群檢測盒 (T-SuRCet)
QY-19542CD2AP (CD2-associated protein)白細胞分化抗原CD2AP
 QY-02000 新的抑癌基因Brg1抗體 Anti-Brg1(brahma-related gene 1)
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 QY-02003 兔抗牛血清白蛋白抗體 Anti-BSA
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