選擇齊一生物的原因：

1.供貨及時。我們訂貨周期短，運輸時間短，在*短時間內為您提供所需產品。

2.價格優(yōu)惠：美國ATCC的中國代理商，沒有中間環(huán)節(jié)。在國內我們保證價格。

3.服務保障：我們擁有專用的技術指導團隊，對每售出的一件產品，提供全面的售前、售中和售后服務。

4.產品種類齊全：25個系統(tǒng)共150多種細胞以及各類菌株，滿足不同客戶的要求。細胞經過嚴格的質控，純度可達98％。常用產品備有現貨。其產品類型如下：消化系統(tǒng)細胞株，呼吸系統(tǒng)細胞株，泌尿系統(tǒng)細胞株生殖系統(tǒng)細胞株 血液循環(huán)系統(tǒng) 內皮細胞細胞株 神經系統(tǒng)及其他細胞株，動物細胞株和菌株

5.對于部分難培養(yǎng)的細胞我公司可代復蘇細胞，并提供技術支持。

6.經驗豐富：我公司是國內專業(yè)化的細胞銷售公司，具有十余年細胞產品銷售經驗，有專業(yè)的銷售團隊和技術人員。齊一生物公司的每一位員工都將竭誠為您服務。

7.質量保證，保證售后

8. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490

【SW480 [SW-480]人結腸癌細胞 】 齊一生物公司專業(yè)代理ScienCell簡介ScienCell Research Laboratories (ScienCell) is a biotechnology company whose mission is the research ａnd development of cell ａnd cell-related products for experimental ａnd therapeutic use. The ScienCell technology provides a variety of high quality normal human ａnd animal cells, cell culture media ａnd reagents, medium supplements, cell-derived RNA, cDNA ａnd proteins for the research community. Scientists at ScienCell are also studying ａnd developing cell therapeutic strategies that will significantly improve the quality of life by achieving the cell＇s ability to combat human degenerative disorders. We are based in San Diego, California ａnd were established in 1999.

【SW480 [SW-480]人結腸癌細胞 】美國ATCC簡介：美國菌種保藏中心， 又稱美國模式菌種收集中心(ATCC)，是位于馬里蘭洲洛克菲勒的一家私營的，非贏利性組織。目前它可以提供各種動植物細胞、標準品、菌株達兩萬多種。ATCC成立于1925年，是世界上的生物資源中心，由美國14家生化、醫(yī)學類行業(yè)協(xié)會組成的理事會負責管理，是一家全球性、非盈利生物標準品資源中心。ATCC向全球發(fā)布其獲取、鑒定、保存及開發(fā)的生物標準品，推動科學研究的驗證、應用及進步 ATCC可以提供以下類別生物標準品：細胞株（3000多種）；菌株（15000多種）；動植物病毒株（2500多種）以及重組物質等。

現已成為可信賴的活體微生物、細胞系等獲得、保存和發(fā)放的國家資源中心。ATCC有29,000多種不同品系可靠的動物細胞和微生物培養(yǎng)體，它能滿足各科學團體對可信賴品系的需要。僅1981年就有39,000多培養(yǎng)物發(fā)放給全世界的科學工作者，并成功地用冷凍和冷凍干燥的方法保存各種細胞品系。 ATCC是一個非(學會單位)無利潤的公司，由各科學組織推選成員組成理事會進行管理。現有工作人員125人。參加的科學團體有：美國免疫協(xié)會、美國生物化學會、美國細胞生物學會、美國微生物學會、美國動物學會、美國遺傳學會、原生動物學會和組織培養(yǎng)協(xié)會等16個學術組織。在ATCC可利用各種材料以及工具和設備進行廣泛的研究。主要研究項目有：酶聯(lián)免疫吸附分析技術(ELISA)，真核細胞轉錄的調控，雜交瘤、淋巴類和髓類以及其他細胞系的建立，人突變細胞系的基因蛋白質，借助計算機進行鑒別分析，培養(yǎng)物的特征鑒別和保存技術的改進,關于化學致癌、致畸和致突變的。

【SW480 [SW-480]人結腸癌細胞 】齊一生物提醒接收到細胞注意事項

1. 接收到細胞，肉眼觀察細胞培養(yǎng)基顏色，顯微鏡觀察細胞生長情況，并對細胞進行不同倍數拍照（建議收到時的培養(yǎng)瓶拍一張照片，顯微鏡拍收到時的細胞100X,200X各一張）。

2.用75%的酒精消毒（建議配置75%的酒精的水是滅菌過的）。

3.嚴格無菌操作，打開細胞培養(yǎng)瓶。

4.將細胞培養(yǎng)瓶內的培養(yǎng)基用吸管完全吸出（嚴禁直接傾倒），放入另一無菌容器中（建議換新的培養(yǎng)基培養(yǎng)細胞）。

5.用PBS 2-3ml/次輕輕洗細胞表面，洗3-4次遍，加入0.05%胰酶-EDTA1-1.5ml,顯微鏡下觀察細胞變圓，輕輕拍打培養(yǎng)瓶直到細胞脫落，加入終止液終止。

6.1000rpm，5min離心，重懸細胞。

7.換新的細胞培養(yǎng)瓶，37℃，5%CO2培養(yǎng)。【SW480 [SW-480]人結腸癌細胞 】

齊一生物銷售：021－6034 8496；181214 53965；173021 04490

69106DNeasy Plant Mini Kit (250)250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)CNY

211015QuantiTect Virus Kit (1000)CNY

206674Type-it CNV SYBR Green PCR +qC Kit (400)CNY

9012763Liquid Handling Kit Plato 1300CNY

59568EpiTect Control DNA (1000)CNY

979014MOTT 16S Primers For PCRCNY

979011PyroMark Q96 Vacuum Prep Trough (5)Re-usable plastic troughs for use in all PyroMark Q96 Vacuum Workstations.CNY

59932EpiTect MethyLight Assay Hs-ONECUT2(100)"10-fold primer TaqMan probe mix, for real-time MethyLight PCR

For TaqMan probe-based real-time quantification of methylated or unmethylated CpG sites"CNY

59695EpiTect PCR Control DNA Set (100)CNY

206343Type-it Mutation Detect PCR Kit (200)"The Type-it Mutation Detect PCR Kit is especially developed for to fast ａnd reliable detection of mutations such as deletions, insertions,and translocations. It is also highly suited for multiplex PCR-based preamplification of SNPs as preparation for genotyping systems like the

SNaPshot Multiplex Kit from ABI

Features

 Successful & reproducible analysis of multiple mutations

 Optimization-free multiplex PCR assay development 

 Specific ａnd sensitive coamplification of all fragments

 Optimized protocol for fast ａnd reliable results"CNY

206341Type-it Mutation Detect PCR Kit (70)"The Type-it Mutation Detect PCR Kit is especially developed for to fast ａnd reliable detection of mutations such as deletions, insertions,and translocations. It is also highly suited for multiplex PCR-based preamplification of SNPs as preparation for genotyping systems like the

SNaPshot Multiplex Kit from ABI

Features

 Successful & reproducible analysis of multiple mutations

 Optimization-free multiplex PCR assay development 

 Specific ａnd sensitive coamplification of all fragments

 Optimized protocol for fast ａnd reliable results"CNY

9012719Set, Pins, (6 small, 4 large)CNY

929561QX Size Marker 50 - 800 bp v2.0 (50 µl)CNY

972804PyroMark Gold Q96 Reagents (5 x 96)"For performing pyrosequencing reaction on the ID (5x 96) ａnd Q96 MD (15x96) 

ID needs more reagents"CNY

301705HiPerFect Transfection Reagent (1 ml)CNY

54362QIAamp cador Pathogen QXT Plus Kit (10)CNY

9012680Peristaltic Pump, 1 channel (A04897)CNY

9012666Pipetting system upgrade 2, BR3000CNY

970480EGFR Pyro Kit (24)CNY

133204NeXtalStock Jeffamine ED 2001 pH 7 (200)CNY

929204QIAxcel Protein Kit (1200)CNY

929004QIAxcel DNA Screening Kit (2400)CNY

9012624Pippeting Station, 2 microtitre platesCNY

206145QIAGEN Multiplex PCR Kit (1000)"contains

1 x Multiplex PCR MasterMix 2x, (1 x 25 ml)

(Mastermix contains HotStarTaq® DNA Polymerase, Multiplex PCR Buffer*, dNTP Mix)

1 x Q-Solution (10ml)

1 x RNase-free water (20 ml)

Handbook"CNY

205311QuantiTect Rev.Transcription Kit (50)"For 50 x 20 µl reactions: gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, RNase-Free Water.

Suitable only for cDNA synthesis followed by real-time analyses."CNY

205211Sensiscript RT Kit (50)For 50 reverse-transcription reactions: Sensiscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free waterCNY

291514mericon Quant RoundUp Ready Soy (48)CNY

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133114NeXtalStock Sodium nitrate (200)CNY

965752QIAamp Media MDx Kit (12)"Reagent Rental" -> price not published on the web or in the catalogCNY