【SAC-IIC3腹水瘤細(xì)胞 】齊一生物科技（上海）有限公司產(chǎn)品已被廣泛應(yīng)用于化學(xué)、化工、生命科學(xué)的基礎(chǔ)研究和開發(fā)應(yīng)用、制藥、疾病診斷與控制、人口與健康、生物技術(shù)等諸多領(lǐng)域.客戶遍布國內(nèi)各大學(xué)、研究所、醫(yī)院、衛(wèi)生防疫、商品檢驗(yàn)檢疫、制藥公司、生物技術(shù)公司和食品工業(yè)等單位. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490；Web:www.qiyibio.com

【SAC-IIC3腹水瘤細(xì)胞 】下面是細(xì)胞株培養(yǎng)的具體無菌技術(shù)要點(diǎn)：

1. 實(shí)驗(yàn)開始前，無菌室和無菌操作臺需要紫外光照射30到60分鐘完全滅菌，用70%ethanol擦拭無菌操作臺面，并且打開無菌操作臺風(fēng)扇運(yùn)行10分鐘之后，才可以進(jìn)行實(shí)驗(yàn)操作。每次操作只能處理一株細(xì)胞株，即使培養(yǎng)基完全相同也不可共用培養(yǎng)基，以避免細(xì)胞間污染或失誤混淆。實(shí)驗(yàn)完成后，請將實(shí)驗(yàn)物品拿出工作臺，用70%ethanol再次擦拭無菌操作臺面。操作間隔應(yīng)該讓無菌操作臺運(yùn)轉(zhuǎn)10分鐘到15分鐘后，才能進(jìn)行下一個細(xì)胞株的操作。

2. 無菌操作工作的區(qū)域應(yīng)保持清潔和寬敞，必要物品（試管架、吸管、吸取器或吸管盒等）可以暫時放置，其它實(shí)驗(yàn)用品使用完畢后應(yīng)移出，有利于空氣流通。實(shí)驗(yàn)用品需70%ethanol擦拭后方可帶入無菌操作臺內(nèi)。實(shí)驗(yàn)操作過程應(yīng)在臺面的中央無菌區(qū)域內(nèi)完成，請勿在邊緣非無菌區(qū)域進(jìn)行操作。

3. 小心取用無菌實(shí)驗(yàn)物品，避免細(xì)菌污染。請勿觸碰吸管尖頭部和容器瓶口處，也不要在打開的容器正上方進(jìn)行操作。容器打開后，請用手夾住瓶蓋并輕握瓶身，傾斜大約45°角取用，盡量不要將瓶蓋口朝上放置于桌面。

4. 工作人員應(yīng)當(dāng)首先注意自身安全，必須穿戴齊實(shí)驗(yàn)衣和實(shí)驗(yàn)手套后才能進(jìn)行實(shí)驗(yàn)。對于病毒感染或是來自人類的細(xì)胞株應(yīng)當(dāng)特別小心操作，并選擇適當(dāng)?shù)燃壍臒o菌操作臺進(jìn)行（至少是Class II）。操作過程中，應(yīng)避免引起aerosol的產(chǎn)生，小心有毒性藥品，例如DMSO和TPA等，并避免針頭的傷害等等。

5. 定期檢測下列項(xiàng)目：

5.1. CO2鋼瓶的CO2壓力

5.2. CO2培養(yǎng)箱的CO2濃度、溫度、和水盤是否有污染（水盤的水需用無菌水，每周定時更換）。

5.3. 無菌操作臺內(nèi)的airflow壓力，定期更換紫外線燈管和HEPA過濾膜，預(yù)濾網(wǎng)（300小時/預(yù)濾網(wǎng)，3000小時/HEPA）。

6. 水槽內(nèi)可添加消毒劑（Zephrin 1:750），需定期更換水槽中的水。

【SAC-IIC3腹水瘤細(xì)胞 】齊一生物友情提醒，本產(chǎn)品僅供科研使用，不得用于臨床及診斷使用！

一細(xì)胞說明，具體詳細(xì)請見細(xì)胞附帶說明書

 二、客戶自備試劑

1、PBS  

2、 2、RPMI-1640 培養(yǎng)基（不含 Hepes）+ 10%胎牛血清+ 1%雙抗

3、0.25%  （W/V）胰蛋白酶（含 0.02% EDTA）

三、細(xì)胞背景

1、生長方式：貼壁

2、種屬：Homo sapiens  人

四、培養(yǎng)條件

1、培養(yǎng)基：RPMI-1640 培養(yǎng)基（不含 Hepes）+ 10%胎牛血清+ 1%雙抗

2、溫度：37.0°C

3、氣體:空氣  95%，CO2 5%

【SAC-IIC3腹水瘤細(xì)胞 】五、培養(yǎng)方法

 收到細(xì)胞后，在倒置顯微鏡下觀察整個細(xì)胞生長情況：

 如果細(xì)胞未長滿，用 75%酒精噴灑整個瓶消毒后放到超菌臺內(nèi)，嚴(yán)格無菌操作，打開細(xì)胞培養(yǎng)瓶，留10ml 培養(yǎng)液繼續(xù)培養(yǎng)。如果細(xì)胞已長滿（達(dá) 80-90%）。即可進(jìn)行傳代，具六、體步驟如下：

1，棄去培養(yǎng)液，用PBS洗 1-2次。

2，向瓶內(nèi)加入 1.0-2.0ml 胰蛋白酶液，在倒置顯微鏡下觀察細(xì)胞消化情況，若細(xì)胞大部分變圓，迅速拿回操作臺，吸取胰蛋白酶，加含有 6ml  含 10%血清的培養(yǎng)液，輕輕吹打細(xì)胞。

3，加入等量的的培養(yǎng)液，輕輕吹打混勻后吸出一半，分到新的培養(yǎng)

4，傳代比例：1:2-1:3  

七、常見問題及解決方案

1、培養(yǎng)瓶有破裂，培養(yǎng)液有漏液：細(xì)胞極大可能會污染，所以我們會及時安排幫老師解決。

2、細(xì)胞漂�。号囵B(yǎng)瓶不開封，瓶口酒精擦拭后平躺放置在培養(yǎng)箱。次日觀察，如細(xì)胞大部分又貼回瓶底，表明細(xì)胞活力正常，剩余漂浮的細(xì)胞可以去掉，留 10ml 培養(yǎng)液培養(yǎng)觀察，細(xì)胞生長至匯合度 80%，進(jìn)行消化傳代；如細(xì)胞還是不貼壁，將細(xì)胞離心收集轉(zhuǎn)到新培養(yǎng)瓶，原培養(yǎng)瓶加部分培養(yǎng)液繼續(xù)培養(yǎng)，中間注意觀察，我們的技術(shù)人員會一直跟蹤指導(dǎo)，直到問題解決。

細(xì)胞培養(yǎng)代理品牌

細(xì)胞培養(yǎng)代理品牌

【SAC-IIC3腹水瘤細(xì)胞 】齊一生物科技（上海）有限公司是一家專業(yè)生產(chǎn)和銷售各種生化試劑,細(xì)胞株、原代細(xì)胞、菌種、醫(yī)藥中間體,標(biāo)準(zhǔn)品和對照品的大型化學(xué)科技公司. 齊一生物銷售：021－6034 8496；181214 53965；173021 04490；Web:www.qiyibio.com

9240241Housing, disposable tip adapter, TRCNY

9239957EZ1, PM ServiceService covers all travel ａnd labor costs plus all necessary parts to perform a Preventative Maintenance. The instrument will also be thoroughly checked ａnd inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.CNY

9239566Packaging, LX100 Optics PlatformCNY

9239558Spring, XYP Torsion Door (Set of 5)CNY

9019925PCB, Mainboard (Microbrd not incl.), CxxCNY

9238867Premium Cover Agreement MDx, 2PM, 1yrThe Premium Cover Agreement MDx, 2PM provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes two annual preventive maintenance visits, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 24 hours (1 working day).CNY

9241213Full Agreement Depot Repair, PtWash, 1yr"The Full Agreement Depot Repair, PtWash provides full service coverage for the plate washer used in the digene HPV application. The service is conducted as an instrument exchange service. 

All shipping, labor ａnd repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."CNY

9241199QIAxcel, Full Agreement, no PMThe Full Depot Repair Agreement, QIAxcel covers all shipping, labor ａnd repair part costs related to an instrument repair for 1 year. Service turn around time is 7-10 working days.CNY

9238821Rect.  carrier for 2 Falcon tubes 50 mlCNY

9019469COMB CASTING 0.2MM X 52 WELLSCNY

9243649Acceler. Valid Support, Other Quant. SysThe QIAGEN Accelerated Validation Support Other Quantification System service includes all travel ａnd labor charges for a site visit up to 5 days by a QIAGEN Applications Specialist.  A QIAGEN specialist will provide hands-on user reaction setup, ａnd best practices.  The Accelerated Validation service includes guidance on the laboratory’s validation experimental design, internal validation documentation support, ａnd QIAGEN sponsored templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  The customer is responsible for final documentation ａnd report using QIAGEN prepared templates provided with the final product. Enough consumable chemistry ａnd accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design ａnd are not included in the cost of validation support. Please indicate which protocols to validate (Plexor HY, Quantifiler Human, Quantifiler Y, Quantifiler Duo)CNY

9233373Spring, gas-driven, cover, BR8000CNY

9233339Valve, throttleCNY

9237335BR MDx, Full AgreementThe Full Agreement MDx provides full service coverage for the BioRobot MDx. Costs for service visits are fully covered including travel, labor, ａnd parts. The agreement also includes one annual preventive maintenance visit, with costs for labor ａnd parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).CNY

9237148XYP FanCNY

9237136#Twister II, Full Agreement, no PMThe Full Agreement BR Twister II provides full service coverage for the BioRobot Twister II. Costs for service visits are fully covered including travel, labor, ａnd parts. With a Full Agreement, there is a priority response time of 48 hours (2 working days).CNY

9237128Upgrade App., RNeasy 96, BR8000CNY

9237119Upgrade App., MagAttract MiniprepCNY

9237105Protocol Dev./Opt., on-site (1-day)The QIAGEN BioRobot Systems Protocol Development/Optimization On-Site Visit includes all travel ａnd labor charges for a site visit up to 8 hours by a QIAGEN Instrumentation Service Specialist to write and/or opimize protocols for the BioRobot System. Parts, consumable chemistries, ａnd accessories not included. Custom protocols are the sole responsibility of the customer ａnd neither warrantied nor guaranteed.CNY

9236419Buzzer, TWICNY

9236411Fingers, deepwell, kit, TWICNY

9232967Board, X-main adapter, BR8000 v1.00CNY

9232951Bar code reader adapter Board, E10084CNY

9236360Syringe Cylinder with Seal"syringe cylinder ａnd seal for Luminex. We apparently do not sell the seal separate from the cylinder. These items are replaced at annual PM＇s, but customer＇s may want to replace them more often ａnd would be responsible for paying for them on their own then.

Source: Glen"CNY

9232920Card, x-main, PCON, v1.04CNY

9232914PCB, RS232, X-mainCNY

9236349375mA Fuse sensor distribution BoardCNY

9236332CompressorCNY

9236322Air SolenoidCNY

9236162O-Ring, replacement kit, RapidPlateCNY